ATE466080T1 - Formulierungen und verfahren zum denaturieren von proteinen - Google Patents

Formulierungen und verfahren zum denaturieren von proteinen

Info

Publication number
ATE466080T1
ATE466080T1 AT04814250T AT04814250T ATE466080T1 AT E466080 T1 ATE466080 T1 AT E466080T1 AT 04814250 T AT04814250 T AT 04814250T AT 04814250 T AT04814250 T AT 04814250T AT E466080 T1 ATE466080 T1 AT E466080T1
Authority
AT
Austria
Prior art keywords
methods
formulations
denaturing proteins
denaturing
proteins
Prior art date
Application number
AT04814250T
Other languages
English (en)
Inventor
Kim Paulsen
Original Assignee
Qiagen North American Holdings
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen North American Holdings filed Critical Qiagen North American Holdings
Application granted granted Critical
Publication of ATE466080T1 publication Critical patent/ATE466080T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/99—Enzyme inactivation by chemical treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
AT04814250T 2003-12-16 2004-12-15 Formulierungen und verfahren zum denaturieren von proteinen ATE466080T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53039203P 2003-12-16 2003-12-16
PCT/US2004/042044 WO2005058933A1 (en) 2003-12-16 2004-12-15 Formulations and methods for denaturing proteins

Publications (1)

Publication Number Publication Date
ATE466080T1 true ATE466080T1 (de) 2010-05-15

Family

ID=34700134

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04814250T ATE466080T1 (de) 2003-12-16 2004-12-15 Formulierungen und verfahren zum denaturieren von proteinen

Country Status (7)

Country Link
US (1) US7115719B2 (de)
EP (1) EP1694692B1 (de)
JP (1) JP4435787B2 (de)
AT (1) ATE466080T1 (de)
CA (1) CA2549985C (de)
DE (1) DE602004026912D1 (de)
WO (1) WO2005058933A1 (de)

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US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
WO2005093052A1 (en) * 2004-03-26 2005-10-06 Fuji Photo Film Co., Ltd. Method for selectively separating and purifying rna and method for separating and purifying nucleic acid
WO2006052680A1 (en) 2004-11-05 2006-05-18 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
DE102005059217B4 (de) * 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
JP4825528B2 (ja) * 2006-02-03 2011-11-30 富士フイルム株式会社 核酸の分離精製方法
CN101460636A (zh) 2006-05-11 2009-06-17 伯克顿迪金森公司 从细胞中提取蛋白质的方法
US9207240B2 (en) 2007-11-14 2015-12-08 Arbor Vita Corporation Method of efficient extraction of protein from cells
EP2281826B1 (de) * 2008-04-02 2016-02-17 Rbc Bioscience Corp. Auf magnetischen cellulosematerialien basierende reagentien, chaotrope mittel, kits und methoden zur isolierung von nukleinsäuren
GB201217405D0 (en) 2012-09-28 2012-11-14 Fermentas Uab Protein removal agent
KR20150128687A (ko) 2013-03-14 2015-11-18 샤이어 휴먼 지네틱 테라피즈 인크. 메신저 rna의 정제 방법
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
EP3578663A1 (de) 2013-03-15 2019-12-11 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
KR102739894B1 (ko) 2014-04-25 2024-12-05 샤이어 휴먼 지네틱 테라피즈 인크. 메신저 rna 의 정제 방법
EP3294885B1 (de) 2015-05-08 2020-07-01 CureVac Real Estate GmbH Verfahren zur herstellung von rna
WO2016193206A1 (en) 2015-05-29 2016-12-08 Curevac Ag A method for producing and purifying rna, comprising at least one step of tangential flow filtration
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
WO2020041793A1 (en) 2018-08-24 2020-02-27 Translate Bio, Inc. Methods for purification of messenger rna
CN110229225B (zh) * 2019-06-12 2021-07-20 山东师范大学 一种南极磷虾中富硫和/或富铯蛋白的制备方法

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US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
AU2002211719B2 (en) * 2001-10-12 2007-06-14 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
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Also Published As

Publication number Publication date
CA2549985A1 (en) 2005-06-30
CA2549985C (en) 2011-05-24
EP1694692A1 (de) 2006-08-30
DE602004026912D1 (de) 2010-06-10
US7115719B2 (en) 2006-10-03
US20050171333A1 (en) 2005-08-04
JP4435787B2 (ja) 2010-03-24
WO2005058933A1 (en) 2005-06-30
EP1694692A4 (de) 2008-07-23
EP1694692B1 (de) 2010-04-28
JP2007515959A (ja) 2007-06-21

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