ATE466099T1 - In situ hybridisierungsverfahren zur entdeckung von zielnukleinsäuren - Google Patents
In situ hybridisierungsverfahren zur entdeckung von zielnukleinsäurenInfo
- Publication number
- ATE466099T1 ATE466099T1 AT99924350T AT99924350T ATE466099T1 AT E466099 T1 ATE466099 T1 AT E466099T1 AT 99924350 T AT99924350 T AT 99924350T AT 99924350 T AT99924350 T AT 99924350T AT E466099 T1 ATE466099 T1 AT E466099T1
- Authority
- AT
- Austria
- Prior art keywords
- sample
- target nucleic
- probe complex
- detecting
- nucleic acids
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 150000007523 nucleic acids Chemical class 0.000 title abstract 6
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 238000007901 in situ hybridization Methods 0.000 title abstract 2
- 239000000523 sample Substances 0.000 abstract 10
- 239000000834 fixative Substances 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- 244000052769 pathogen Species 0.000 abstract 2
- 241000238876 Acari Species 0.000 abstract 1
- 241000223836 Babesia Species 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 abstract 1
- 206010036790 Productive cough Diseases 0.000 abstract 1
- JCGCKSUCGVTMNB-UHFFFAOYSA-N acetic acid;formaldehyde Chemical compound O=C.CC(O)=O JCGCKSUCGVTMNB-UHFFFAOYSA-N 0.000 abstract 1
- ZCHPKWUIAASXPV-UHFFFAOYSA-N acetic acid;methanol Chemical compound OC.CC(O)=O ZCHPKWUIAASXPV-UHFFFAOYSA-N 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 abstract 1
- 210000004251 human milk Anatomy 0.000 abstract 1
- 235000020256 human milk Nutrition 0.000 abstract 1
- 238000000386 microscopy Methods 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 210000003802 sputum Anatomy 0.000 abstract 1
- 208000024794 sputum Diseases 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8856198P | 1998-05-18 | 1998-05-18 | |
| PCT/US1999/011046 WO1999060163A1 (en) | 1998-05-18 | 1999-05-18 | In situ hybridization method for detecting target nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE466099T1 true ATE466099T1 (de) | 2010-05-15 |
Family
ID=22212089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99924350T ATE466099T1 (de) | 1998-05-18 | 1999-05-18 | In situ hybridisierungsverfahren zur entdeckung von zielnukleinsäuren |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6165723A (de) |
| EP (1) | EP1080228B1 (de) |
| AT (1) | ATE466099T1 (de) |
| AU (1) | AU4087399A (de) |
| CA (1) | CA2332510C (de) |
| CY (1) | CY1110164T1 (de) |
| DE (1) | DE69942309D1 (de) |
| DK (1) | DK1080228T3 (de) |
| ES (1) | ES2344873T3 (de) |
| PT (1) | PT1080228E (de) |
| WO (1) | WO1999060163A1 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1005633T3 (da) | 1997-08-20 | 2008-12-15 | Univ Miami | Vævsfikserings-dehydratiserings-fedtfjernelses-imprægneringsmetode med höj kvalitet og kontinuerligt gennemlöb |
| US6329149B1 (en) * | 2000-03-07 | 2001-12-11 | Janice Cori Cobb | Reverse-root-canal method for extracting aDNA |
| US20040115692A1 (en) * | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
| US7439346B2 (en) | 2001-10-12 | 2008-10-21 | Perkinelmer Las Inc. | Nucleic acids arrays and methods of use therefor |
| US7138226B2 (en) * | 2002-05-10 | 2006-11-21 | The University Of Miami | Preservation of RNA and morphology in cells and tissues |
| JP2004135509A (ja) * | 2002-10-15 | 2004-05-13 | Hitachi Ltd | 遺伝子発現解析法及び遺伝子発現解析用プローブキット |
| US8062897B2 (en) | 2003-07-21 | 2011-11-22 | Aureon Laboratories, Inc. | Diagnostic histopathology using multiplex gene expression FISH |
| EP1676117B1 (de) | 2003-10-24 | 2009-09-09 | The University Of Miami | Vereinfachte gewebeverarbeitung |
| US8911942B2 (en) | 2004-05-20 | 2014-12-16 | Quest Diagnostics Investments Incorporated | Single label comparative hybridization |
| JP4424155B2 (ja) * | 2004-10-27 | 2010-03-03 | 富士通株式会社 | 移動通信システム、移動局 |
| EP1846760B1 (de) | 2005-01-27 | 2010-12-15 | Quest Diagnostics Investments Incorporated | Schnelle komparative genomhybridisierung |
| US8758990B2 (en) * | 2005-07-28 | 2014-06-24 | Id-Fish Technology, Inc. | Methods for detecting and differentiating Mycobacterium genus and Mycobacterium avium complex in a sample or culture |
| BRPI0615958B8 (pt) | 2005-07-28 | 2021-05-25 | Id Fish Tech Inc | composição para aumentar a permeabilidade de paredes celulares e membranas celulares de mycobacterium sp. e método para detectar um alvo em uma célula |
| US8076074B2 (en) | 2005-11-29 | 2011-12-13 | Quest Diagnostics Investments Incorporated | Balanced translocation in comparative hybridization |
| US8530196B2 (en) * | 2006-04-07 | 2013-09-10 | The Trustees Of The Univeristy Of Pennsylvania | In situ cloning from pathological tissue specimens |
| US8323901B2 (en) * | 2006-11-30 | 2012-12-04 | Id-Fish Technology, Inc. | Nucleic acid probes and methods for detecting plasmodium parasites |
| BRPI0717707B8 (pt) * | 2006-11-30 | 2021-07-27 | Id Fish Tech Inc | método para detectar a presença de plasmodium em uma amostra, fragmento de ácido nucléico e método para detectar e diferenciar espécies de protozoários |
| US7807359B2 (en) | 2006-12-01 | 2010-10-05 | Quest Diagnostics Investments Incorporated | Methods of detecting TPMT mutations |
| US7507539B2 (en) | 2007-07-30 | 2009-03-24 | Quest Diagnostics Investments Incorporated | Substractive single label comparative hybridization |
| US8093063B2 (en) | 2007-11-29 | 2012-01-10 | Quest Diagnostics Investments Incorporated | Assay for detecting genetic abnormalities in genomic nucleic acids |
| KR101020844B1 (ko) * | 2008-09-04 | 2011-03-09 | 삼성전기주식회사 | 구리 나노입자의 저온 환원 소결을 위한 환원제 및 이를이용한 저온 소결 방법 |
| US8039794B2 (en) | 2008-12-16 | 2011-10-18 | Quest Diagnostics Investments Incorporated | Mass spectrometry assay for thiopurine-S-methyl transferase activity and products generated thereby |
| US20110086772A1 (en) | 2009-09-25 | 2011-04-14 | Signature Genomics Laboratories Llc | Multiplex (+/-) stranded arrays and assays for detecting chromosomal abnormalities associated with cancer and other diseases |
| ES2465948T3 (es) * | 2010-04-30 | 2014-06-09 | Exiqon A/S | Procedimiento de hibridación in situ y tampón |
| WO2017041005A1 (en) | 2015-09-03 | 2017-03-09 | Abbott Molecular Inc. | Hybridization buffers comprising an alkyl diester |
| CN108779490A (zh) | 2016-01-08 | 2018-11-09 | 雅培分子公司 | 包含硫氰酸胍的杂交缓冲液 |
| US11667978B2 (en) * | 2017-06-07 | 2023-06-06 | Gen-Probe Incorporated | Detecting Babesia species nucleic acid in a sample |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5447841A (en) * | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US4886741A (en) * | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
| WO1990002173A1 (en) * | 1988-08-31 | 1990-03-08 | Research Development Foundation | One-step in situ hybridization assay |
| US5264343A (en) * | 1990-08-31 | 1993-11-23 | Eleanor Roosevelt Institute | Method for distinguishing normal and cancer cells |
| US5627029A (en) * | 1991-05-03 | 1997-05-06 | Genzyme Corporation | Method for obtaining informative cells |
| US5506098A (en) * | 1991-09-04 | 1996-04-09 | Daikin Industries, Ltd. | In situ hybridization method |
| EP0662151A1 (de) * | 1992-07-17 | 1995-07-12 | Aprogenex, Inc. | Verbindungen, die den bei fluorimetrischen in-situ verfahren durch proben auftretenden hintergrund reduzieren |
| US5629147A (en) * | 1992-07-17 | 1997-05-13 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
| US5521061A (en) * | 1992-07-17 | 1996-05-28 | Aprogenex, Inc. | Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies |
| US5449604A (en) * | 1992-10-21 | 1995-09-12 | University Of Washington | Chromosome 14 and familial Alzheimers disease genetic markers and assays |
| US5582985A (en) * | 1994-08-05 | 1996-12-10 | The Regents Of The University Of California | Detection of mycobacteria |
| US5750340A (en) * | 1995-04-07 | 1998-05-12 | University Of New Mexico | In situ hybridization solution and process |
| US5888733A (en) * | 1995-11-16 | 1999-03-30 | Dako A/S | In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples |
-
1999
- 1999-05-18 AT AT99924350T patent/ATE466099T1/de active
- 1999-05-18 CA CA2332510A patent/CA2332510C/en not_active Expired - Lifetime
- 1999-05-18 US US09/313,852 patent/US6165723A/en not_active Expired - Lifetime
- 1999-05-18 DE DE69942309T patent/DE69942309D1/de not_active Expired - Lifetime
- 1999-05-18 EP EP99924350A patent/EP1080228B1/de not_active Expired - Lifetime
- 1999-05-18 ES ES99924350T patent/ES2344873T3/es not_active Expired - Lifetime
- 1999-05-18 PT PT99924350T patent/PT1080228E/pt unknown
- 1999-05-18 DK DK99924350.4T patent/DK1080228T3/da active
- 1999-05-18 WO PCT/US1999/011046 patent/WO1999060163A1/en not_active Ceased
- 1999-05-18 AU AU40873/99A patent/AU4087399A/en not_active Abandoned
-
2010
- 2010-06-28 CY CY20101100590T patent/CY1110164T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT1080228E (pt) | 2010-07-22 |
| CA2332510A1 (en) | 1999-11-25 |
| DK1080228T3 (da) | 2010-08-16 |
| EP1080228A4 (de) | 2005-01-12 |
| ES2344873T3 (es) | 2010-09-08 |
| EP1080228B1 (de) | 2010-04-28 |
| AU4087399A (en) | 1999-12-06 |
| CA2332510C (en) | 2011-07-26 |
| DE69942309D1 (de) | 2010-06-10 |
| US6165723A (en) | 2000-12-26 |
| EP1080228A1 (de) | 2001-03-07 |
| CY1110164T1 (el) | 2015-01-14 |
| WO1999060163A1 (en) | 1999-11-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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