ATE474936T1 - Schnelle synthese von oligonukleotiden - Google Patents

Schnelle synthese von oligonukleotiden

Info

Publication number
ATE474936T1
ATE474936T1 AT05785221T AT05785221T ATE474936T1 AT E474936 T1 ATE474936 T1 AT E474936T1 AT 05785221 T AT05785221 T AT 05785221T AT 05785221 T AT05785221 T AT 05785221T AT E474936 T1 ATE474936 T1 AT E474936T1
Authority
AT
Austria
Prior art keywords
dna
stranded
double
target
primary
Prior art date
Application number
AT05785221T
Other languages
English (en)
Inventor
Michael Sussman
Kathryn Richmond
Matt Rodesch
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Application granted granted Critical
Publication of ATE474936T1 publication Critical patent/ATE474936T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AT05785221T 2004-06-09 2005-06-08 Schnelle synthese von oligonukleotiden ATE474936T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57819504P 2004-06-09 2004-06-09
PCT/US2005/020204 WO2005123956A2 (en) 2004-06-09 2005-06-08 Rapid production of oligonucleotides

Publications (1)

Publication Number Publication Date
ATE474936T1 true ATE474936T1 (de) 2010-08-15

Family

ID=35510339

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05785221T ATE474936T1 (de) 2004-06-09 2005-06-08 Schnelle synthese von oligonukleotiden

Country Status (8)

Country Link
US (2) US20100016178A1 (de)
EP (1) EP1759019B1 (de)
JP (1) JP2008502367A (de)
AT (1) ATE474936T1 (de)
AU (1) AU2005254972B2 (de)
CA (1) CA2567735C (de)
DE (1) DE602005022443D1 (de)
WO (1) WO2005123956A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011066186A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
EP2630264A4 (de) * 2010-10-22 2014-04-02 Harvard College Orthogonale amplifikation und anordnung von nukleinsäuresequenzen
WO2012078312A2 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US9340826B2 (en) 2011-08-01 2016-05-17 Celemics, Inc. Method of preparing nucleic acid molecules
EP2748318B1 (de) * 2011-08-26 2015-11-04 Gen9, Inc. Zusammensetzungen und verfahren für hochqualitative anordnung von nukleinsäuren
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
US11060137B2 (en) * 2016-12-14 2021-07-13 Codex Dna, Inc. Methods for assembling DNA molecules

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
AU746760B2 (en) * 1998-02-23 2002-05-02 Wisconsin Alumni Research Foundation Method and apparatus for synthesis of arrays of DNA probes
DE50015858D1 (de) * 1999-02-19 2010-03-18 Febit Holding Gmbh Verfahren zur Herstellung von Polymeren
WO2002095073A1 (en) * 2001-05-18 2002-11-28 Wisconsin Alumni Research Foundation Method for the synthesis of dna sequences
WO2003072832A1 (en) * 2002-02-28 2003-09-04 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20030180781A1 (en) * 2002-03-25 2003-09-25 The Regents Of The University Of California Constructing very long DNA sequences from synthetic DNA molecules
JP3911185B2 (ja) * 2002-04-05 2007-05-09 株式会社ニフコ 過給油防止バルブ
US7135310B2 (en) * 2002-04-24 2006-11-14 The Regents Of The University Of California Method to amplify variable sequences without imposing primer sequences
US6815167B2 (en) * 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
WO2005089110A2 (en) 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis

Also Published As

Publication number Publication date
US20100016178A1 (en) 2010-01-21
CA2567735A1 (en) 2005-12-29
AU2005254972B2 (en) 2010-11-04
WO2005123956A3 (en) 2006-06-29
AU2005254972A1 (en) 2005-12-29
CA2567735C (en) 2015-04-28
US20150284767A1 (en) 2015-10-08
EP1759019B1 (de) 2010-07-21
EP1759019A2 (de) 2007-03-07
WO2005123956A2 (en) 2005-12-29
JP2008502367A (ja) 2008-01-31
DE602005022443D1 (de) 2010-09-02
US9206473B2 (en) 2015-12-08

Similar Documents

Publication Publication Date Title
ATE474936T1 (de) Schnelle synthese von oligonukleotiden
Dominic Mills et al. Strand-specific RNA-Seq provides greater resolution of transcriptome profiling
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
RU2019106038A (ru) Способы de novo сборки баркодированных фрагментов геномной днк
JP2007537751A5 (de)
MXPA05001355A (es) Formas nuevas adicionales de moleculas de arn de interferencia.
WO2004015062A3 (en) Methods and compositions relating to gene silencing
WO2002052031A3 (en) Nucleic acid amplification
RU2017101329A (ru) Опосредованная нуклеазой сборка днк
DK2376517T3 (da) Transposon-ende-sammensætninger og fremgangsmåder til at modificere nukleinsyrer
DE602005020691D1 (de) Rna-amplifikation auf ligationsbasis
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
ATE414767T1 (de) Nukleinsäure-linker und deren verwendung in der gensynthese
EP1750771A4 (de) Pankreasinsel-mikro-rna und verfahren zu ihrer unterdrückung
WO2005089287A3 (en) Methods and compositions for the specific inhibition of gene expression by double-stranded rna
ATE494370T1 (de) Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren
DE60039772D1 (de) Verfahren zur amplifikation von signal-flankierend
CN101532014A (zh) 对目标基因组区域边扩增边连接的公用接头及连接方法
WO2003072798A3 (en) Method for generating amplified rna
PT1171634E (pt) Pares de iniciadores de amplificacao e de sequenciacao e sua utilizacao
WO2005074511A3 (en) Methods and compositions for homozygous gene inactivation using collections of pre-defined nucleotide sequences complementary to chromosomal transcripts
DE69835299D1 (de) Transkriptionsbasierende vervielfältigung von dopplesträngigen dns-zielmolekülen
EP1333089A4 (de) Verfahren zur synthese einzelsträngiger nukleinsäure
WO2005116213A3 (en) Directed evolution of proteins
WO2003004702A3 (en) Method for determining chromatin structure

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties