ATE474936T1 - Schnelle synthese von oligonukleotiden - Google Patents
Schnelle synthese von oligonukleotidenInfo
- Publication number
- ATE474936T1 ATE474936T1 AT05785221T AT05785221T ATE474936T1 AT E474936 T1 ATE474936 T1 AT E474936T1 AT 05785221 T AT05785221 T AT 05785221T AT 05785221 T AT05785221 T AT 05785221T AT E474936 T1 ATE474936 T1 AT E474936T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- stranded
- double
- target
- primary
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 6
- 102000053602 DNA Human genes 0.000 abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57819504P | 2004-06-09 | 2004-06-09 | |
| PCT/US2005/020204 WO2005123956A2 (en) | 2004-06-09 | 2005-06-08 | Rapid production of oligonucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE474936T1 true ATE474936T1 (de) | 2010-08-15 |
Family
ID=35510339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05785221T ATE474936T1 (de) | 2004-06-09 | 2005-06-08 | Schnelle synthese von oligonukleotiden |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20100016178A1 (de) |
| EP (1) | EP1759019B1 (de) |
| JP (1) | JP2008502367A (de) |
| AT (1) | ATE474936T1 (de) |
| AU (1) | AU2005254972B2 (de) |
| CA (1) | CA2567735C (de) |
| DE (1) | DE602005022443D1 (de) |
| WO (1) | WO2005123956A2 (de) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
| US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
| US10207240B2 (en) | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
| EP3597771A1 (de) | 2009-11-25 | 2020-01-22 | Gen9, Inc. | Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion |
| WO2011066185A1 (en) | 2009-11-25 | 2011-06-03 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
| WO2011085075A2 (en) | 2010-01-07 | 2011-07-14 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
| EP2630264A4 (de) * | 2010-10-22 | 2014-04-02 | Harvard College | Orthogonale amplifikation und anordnung von nukleinsäuresequenzen |
| CN103502448B (zh) | 2010-11-12 | 2017-03-29 | Gen9股份有限公司 | 核酸合成的方法和设备 |
| WO2012064975A1 (en) | 2010-11-12 | 2012-05-18 | Gen9, Inc. | Protein arrays and methods of using and making the same |
| KR101454886B1 (ko) * | 2011-08-01 | 2014-11-03 | 주식회사 셀레믹스 | 핵산분자의 제조방법 |
| LT2944693T (lt) * | 2011-08-26 | 2019-08-26 | Gen9, Inc. | Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai |
| US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
| CA2871505C (en) | 2012-04-24 | 2021-10-12 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
| IL236303B (en) | 2012-06-25 | 2022-07-01 | Gen9 Inc | Methods for high-throughput nucleic acid assembly and sequencing |
| US11060137B2 (en) * | 2016-12-14 | 2021-07-13 | Codex Dna, Inc. | Methods for assembling DNA molecules |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6670127B2 (en) * | 1997-09-16 | 2003-12-30 | Egea Biosciences, Inc. | Method for assembly of a polynucleotide encoding a target polypeptide |
| US6375903B1 (en) * | 1998-02-23 | 2002-04-23 | Wisconsin Alumni Research Foundation | Method and apparatus for synthesis of arrays of DNA probes |
| WO2000049142A1 (de) * | 1999-02-19 | 2000-08-24 | Febit Ferrarius Biotechnology Gmbh | Verfahren zur herstellung von polymeren |
| CA2447240C (en) * | 2001-05-18 | 2013-02-19 | Wisconsin Alumni Research Foundation | Method for the synthesis of dna sequences |
| IL163788A0 (en) * | 2002-02-28 | 2005-12-18 | Wisconsin Alumni Res Found | Method of error reduction in nucleic acid populations |
| US20030180781A1 (en) * | 2002-03-25 | 2003-09-25 | The Regents Of The University Of California | Constructing very long DNA sequences from synthetic DNA molecules |
| JP3911185B2 (ja) * | 2002-04-05 | 2007-05-09 | 株式会社ニフコ | 過給油防止バルブ |
| US7135310B2 (en) * | 2002-04-24 | 2006-11-14 | The Regents Of The University Of California | Method to amplify variable sequences without imposing primer sequences |
| US6815167B2 (en) * | 2002-04-25 | 2004-11-09 | Geneohm Sciences | Amplification of DNA to produce single-stranded product of defined sequence and length |
| US7563600B2 (en) * | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
| AU2005222788A1 (en) | 2004-02-27 | 2005-09-29 | President And Fellows Of Harvard College | Polynucleotide synthesis |
-
2005
- 2005-06-08 AU AU2005254972A patent/AU2005254972B2/en not_active Ceased
- 2005-06-08 AT AT05785221T patent/ATE474936T1/de not_active IP Right Cessation
- 2005-06-08 DE DE602005022443T patent/DE602005022443D1/de not_active Expired - Lifetime
- 2005-06-08 US US11/148,401 patent/US20100016178A1/en not_active Abandoned
- 2005-06-08 CA CA2567735A patent/CA2567735C/en not_active Expired - Fee Related
- 2005-06-08 EP EP05785221A patent/EP1759019B1/de not_active Expired - Lifetime
- 2005-06-08 JP JP2007527702A patent/JP2008502367A/ja active Pending
- 2005-06-08 WO PCT/US2005/020204 patent/WO2005123956A2/en not_active Ceased
-
2011
- 2011-11-01 US US13/286,932 patent/US9206473B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1759019A2 (de) | 2007-03-07 |
| CA2567735C (en) | 2015-04-28 |
| AU2005254972B2 (en) | 2010-11-04 |
| US20150284767A1 (en) | 2015-10-08 |
| WO2005123956A2 (en) | 2005-12-29 |
| US20100016178A1 (en) | 2010-01-21 |
| EP1759019B1 (de) | 2010-07-21 |
| AU2005254972A1 (en) | 2005-12-29 |
| WO2005123956A3 (en) | 2006-06-29 |
| JP2008502367A (ja) | 2008-01-31 |
| DE602005022443D1 (de) | 2010-09-02 |
| CA2567735A1 (en) | 2005-12-29 |
| US9206473B2 (en) | 2015-12-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |