ATE478687T1 - SINGLE STRANDED OLIGONUCLEOTIDE VECTORS SUITABLE FOR MUTATION - Google Patents
SINGLE STRANDED OLIGONUCLEOTIDE VECTORS SUITABLE FOR MUTATIONInfo
- Publication number
- ATE478687T1 ATE478687T1 AT00959442T AT00959442T ATE478687T1 AT E478687 T1 ATE478687 T1 AT E478687T1 AT 00959442 T AT00959442 T AT 00959442T AT 00959442 T AT00959442 T AT 00959442T AT E478687 T1 ATE478687 T1 AT E478687T1
- Authority
- AT
- Austria
- Prior art keywords
- effective
- oligodeoxynucleotides
- mutation
- single stranded
- stranded oligonucleotide
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- 230000035772 mutation Effects 0.000 title 1
- 239000013598 vector Substances 0.000 title 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 abstract 4
- 210000004027 cell Anatomy 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 108091028664 Ribonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 210000003494 hepatocyte Anatomy 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000002336 ribonucleotide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention concerns the introduction of predetermined genetic changes in target genes of a living cell by introducing an oligodeoxynucleotide encoding the predetermined change. The oligodeoxynucleotides are effective in animal, plant and bacterial cells. Specific end modifications that greatly increase the effectiveness of the oligodeoxynucleotides in bacteria are described. Surprisingly, unmodified oligodeoxynucleotides can be as effective in mammalian cells, including in vivo hepatocytes, as the modified nucleotides and can be as effective or more effective than chimeric oligonucleotides that consist of a mixture of deoxynucleotides and 2'-O-methyl ribonucleotides.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/384,960 US6271360B1 (en) | 1999-08-27 | 1999-08-27 | Single-stranded oligodeoxynucleotide mutational vectors |
| PCT/US2000/023457 WO2001015740A1 (en) | 1999-08-27 | 2000-08-25 | Single-stranded oligodeoxynucleotide mutational vectors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE478687T1 true ATE478687T1 (en) | 2010-09-15 |
Family
ID=23519459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00959442T ATE478687T1 (en) | 1999-08-27 | 2000-08-25 | SINGLE STRANDED OLIGONUCLEOTIDE VECTORS SUITABLE FOR MUTATION |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US6271360B1 (en) |
| EP (3) | EP2266627B1 (en) |
| JP (2) | JP5697187B2 (en) |
| CN (1) | CN1384759B (en) |
| AT (1) | ATE478687T1 (en) |
| AU (2) | AU773242C (en) |
| BR (1) | BR0013590A (en) |
| CA (1) | CA2382120C (en) |
| DE (1) | DE60044876D1 (en) |
| DK (3) | DK1210123T3 (en) |
| ES (3) | ES2358292T3 (en) |
| NZ (1) | NZ517942A (en) |
| PT (3) | PT2266627E (en) |
| WO (1) | WO2001015740A1 (en) |
| ZA (1) | ZA200201228B (en) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6652607B2 (en) | 1999-07-07 | 2003-11-25 | The Lubrizol Corporation | Concentrated emulsion for making an aqueous hydrocarbon fuel |
| EP1212406A1 (en) * | 1999-08-20 | 2002-06-12 | University Of Delaware | Cell-free assay for plant gene targeting and conversion |
| US6271360B1 (en) * | 1999-08-27 | 2001-08-07 | Valigen (Us), Inc. | Single-stranded oligodeoxynucleotide mutational vectors |
| AR025996A1 (en) | 1999-10-07 | 2002-12-26 | Valigen Us Inc | NON-TRANSGENIC PLANTS RESISTANT TO HERBICIDES. |
| US6936467B2 (en) * | 2000-03-27 | 2005-08-30 | University Of Delaware | Targeted chromosomal genomic alterations with modified single stranded oligonucleotides |
| EP1268768A2 (en) * | 2000-03-27 | 2003-01-02 | University Of Delaware | Targeted chromosomal genomic alterations with modified single stranded oligonucleotides |
| AU2001263271A1 (en) * | 2000-05-17 | 2001-11-26 | University Of Delaware | Plant gene targeting using oligonucleotides |
| NZ524366A (en) * | 2000-07-27 | 2005-12-23 | Univ Delaware | Methods for enhancing targeted gene alteration using oligonucleotides |
| US7053195B1 (en) * | 2001-06-12 | 2006-05-30 | Syngenta Participatious Ag | Locked nucleic acid containing heteropolymers and related methods |
| WO2003013437A2 (en) * | 2001-08-07 | 2003-02-20 | University Of Delaware | Compositions and methods for the prevention and treatment of huntington's disease |
| US20040096880A1 (en) * | 2001-08-07 | 2004-05-20 | Kmiec Eric B. | Compositions and methods for the treatment of diseases exhibiting protein misassembly and aggregation |
| EP1448766A2 (en) * | 2001-09-27 | 2004-08-25 | University Of Delaware | Coisogenic eukaryotic cell collections |
| EP1446503A2 (en) * | 2001-09-27 | 2004-08-18 | University Of Delaware | Composition and methods for enhancing oligonucleotide-directed nucleic acid sequence alteration |
| WO2003075856A2 (en) * | 2002-03-07 | 2003-09-18 | University Of Delaware | Methods, compositions, and kits for enhancing oligonucleotide-mediated nucleic acid sequence alteration using compositions comprising a histone deacetylase inhibitor, lambda phage beta protein, or hydroxyurea |
| GB0218803D0 (en) * | 2002-08-13 | 2002-09-18 | Nl Kanker I | Targeted gene modification by single-standed DNA oligonucleotides |
| AU2003282477A1 (en) * | 2002-10-07 | 2004-05-04 | Napro Bio Therapeutics, Inc. | Methods and compositions for reducing screening in oligonucleotide-directed nucleic acid sequence alteration |
| US6929917B2 (en) * | 2002-11-18 | 2005-08-16 | Pregentis | Method for cloning of a rare, specifically mutated cell |
| US20070072815A1 (en) * | 2004-05-04 | 2007-03-29 | Kmiec Eric B | Methods and kits to increase the efficiency of oligonucleotide-directed nucleic acid sequence alteration |
| CA2636771C (en) | 2006-01-12 | 2016-05-24 | Greg F.W. Gocal | Epsps mutants |
| CA2893168C (en) | 2006-06-28 | 2017-11-07 | Nucelis Inc. | Fatty acid blends and uses therefor |
| EP2182056B1 (en) | 2006-10-06 | 2015-12-23 | BASF Plant Science GmbH | Method for producing polyunsaturated fatty acids in transgenic non-human organisms |
| US9102948B2 (en) | 2006-11-17 | 2015-08-11 | 22Nd Century Limited, Llc | Regulating alkaloids |
| CN108130336A (en) * | 2007-10-05 | 2018-06-08 | 赛布斯欧洲公司 | The acetohydroxy acid synthase gene being mutated in Btassica |
| US20110023352A1 (en) * | 2007-12-27 | 2011-02-03 | Knuth Mark E | Alkylester fatty acid blends and uses therefor |
| JP2011520471A (en) * | 2008-05-23 | 2011-07-21 | サイバス オイルズ，エルエルシー | Production of squalene using yeast |
| CN102216453B (en) | 2008-09-26 | 2014-02-05 | 巴斯夫农化产品有限公司 | Herbicide-resistant AHAS-mutants and methods of use |
| DE112009003708T5 (en) | 2008-12-12 | 2012-09-13 | Basf Plant Science Gmbh | Desaturases and methods of producing polyunsaturated fatty acids in transgenic organisms |
| CN102666837A (en) | 2009-11-23 | 2012-09-12 | 纽塞利斯公司 | Methods and compositions for producing squalene using yeast |
| US20120122223A1 (en) | 2010-08-03 | 2012-05-17 | Cibus Us Llc | Mutated protoporphyrinogen ix oxidase (ppx) genes |
| UA112969C2 (en) | 2010-08-03 | 2016-11-25 | Сібас Юс Ллс | PLANT RESISTANT TO ONE OR MORE PPH-INHIBITING HERBICIDES CONTAINING PROTOPORPHYRINOGEN IX OXIDASE (PPX) MUTANT GENE |
| WO2012028673A1 (en) | 2010-09-02 | 2012-03-08 | Ludwig-Maximilians-Universitaet Muenchen | Spontaneous organogenesis in plants |
| EP2426204A1 (en) | 2010-09-02 | 2012-03-07 | Ludwig-Maximilians-Universität München | Spontaneous nodule organogenesis in plants |
| KR101137285B1 (en) | 2010-10-28 | 2012-04-20 | 위월드 주식회사 | Micro antenna feeder for wide band |
| KR20150041788A (en) | 2012-08-17 | 2015-04-17 | 다우 아그로사이언시즈 엘엘씨 | Use of a maize untranslated region for transgene expression in plants |
| EA037142B1 (en) | 2013-03-14 | 2021-02-10 | Сибас Юс Ллс | Mutated allene oxide synthase 2 (aos2) genes |
| CA2905135C (en) | 2013-03-15 | 2022-10-25 | Cibus Us Llc | Targeted gene modification using oligonucleotide-mediated gene repair |
| US12331303B2 (en) | 2013-03-15 | 2025-06-17 | Cibus Us Llc | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| EP4136963B1 (en) | 2013-03-15 | 2026-01-21 | Cibus US LLC | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| US9957515B2 (en) | 2013-03-15 | 2018-05-01 | Cibus Us Llc | Methods and compositions for targeted gene modification |
| KR102450868B1 (en) | 2014-03-14 | 2022-10-06 | 시버스 유에스 엘엘씨 | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| CN109072248A (en) | 2016-02-09 | 2018-12-21 | 希博斯美国有限公司 | Methods and compositions for increasing the efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| US10584363B2 (en) | 2016-06-03 | 2020-03-10 | Takara Bio Usa, Inc. | Methods of producing and using single-stranded deoxyribonucleic acids and compositions for use in practicing the same |
| CN118830491A (en) | 2018-01-09 | 2024-10-25 | 希博斯美国有限公司 | Shatterproof genes and mutations |
| UA129286C2 (en) | 2018-04-04 | 2025-03-12 | Сайбас Юес Ллс | FAD2 GENES AND MUTATIONS |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2731844B2 (en) * | 1987-04-22 | 1998-03-25 | ザ・ユニヴァーシティー・オブ・コネチカット | Carrier for introducing polynucleotide into mammalian cells, water-soluble polymer complex and method for introducing the same |
| DE59108644D1 (en) * | 1990-07-02 | 1997-05-07 | Hoechst Ag | Oligonucleotide analogs with terminal 3'-3 'or. 5'-5 'internucleotide linkages |
| EP0666923A1 (en) | 1991-09-05 | 1995-08-16 | The University Of Connecticut | Targeted delivery of poly- or oligonucleotides to cells |
| US5556959A (en) | 1993-01-22 | 1996-09-17 | Pharmacia P-L Biochemicals Inc. | Indocarbocyanine-linked phosphoramidites |
| US5808044A (en) * | 1993-01-22 | 1998-09-15 | Pharmacia Biotech Inc. | Indocarbocyanine and benzindocarbocyanine phosphoramidites |
| DE733059T1 (en) | 1993-12-09 | 1997-08-28 | Univ Jefferson | CONNECTIONS AND METHOD FOR LOCATION-SPECIFIC MUTATION IN EUKARYOTIC CELLS |
| US5780296A (en) | 1995-01-17 | 1998-07-14 | Thomas Jefferson University | Compositions and methods to promote homologous recombination in eukaryotic cells and organisms |
| WO1996040271A1 (en) * | 1995-06-07 | 1996-12-19 | Yale University | Treatment of hemoglobinopathies |
| US5908777A (en) * | 1995-06-23 | 1999-06-01 | University Of Pittsburgh | Lipidic vector for nucleic acid delivery |
| FR2736926B1 (en) | 1995-07-19 | 1997-08-22 | Rhone Poulenc Agrochimie | 5-ENOL PYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE MUTEE, CODING GENE FOR THIS PROTEIN AND PROCESSED PLANTS CONTAINING THIS GENE |
| US5760012A (en) | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
| US5888983A (en) | 1996-05-01 | 1999-03-30 | Thomas Jefferson University | Method and oligonucleobase compounds for curing diseases caused by mutations |
| US5731181A (en) | 1996-06-17 | 1998-03-24 | Thomas Jefferson University | Chimeric mutational vectors having non-natural nucleotides |
| CA2282729A1 (en) * | 1997-02-26 | 1998-09-03 | The General Hospital Corporation | Transgenic animals and cell lines for screening drugs effective for the treatment or prevention of alzheimer's disease |
| EP0979311A1 (en) | 1997-04-30 | 2000-02-16 | Of The University Of Minnesota Regents | $i(IN VIVO) USE OF RECOMBINAGENIC OLIGONUCLEOBASES TO CORRECT GENETIC LESIONS IN HEPATOCYTES |
| US6524613B1 (en) | 1997-04-30 | 2003-02-25 | Regents Of The University Of Minnesota | Hepatocellular chimeraplasty |
| GB9711015D0 (en) | 1997-05-28 | 1997-07-23 | Zeneca Ltd | Improvements in or relating to organic compounds |
| US5986086A (en) * | 1997-06-20 | 1999-11-16 | Amersham Pharmacia Biotech Inc. | Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides |
| US7094606B2 (en) * | 1997-08-05 | 2006-08-22 | Arntzen Charles J | Use of mixed duplex oligonucleotides to effect localized genetic changes in plants |
| CA2309719A1 (en) | 1997-11-18 | 1999-05-27 | Pioneer Hi-Bred International, Inc. | Targeted manipulation of genes in plants |
| US6004804A (en) | 1998-05-12 | 1999-12-21 | Kimeragen, Inc. | Non-chimeric mutational vectors |
| US6271360B1 (en) * | 1999-08-27 | 2001-08-07 | Valigen (Us), Inc. | Single-stranded oligodeoxynucleotide mutational vectors |
| EP3183223A1 (en) | 2014-08-21 | 2017-06-28 | Corning Incorporated | Methods for preventing blisters in laminated glass articles and laminated glass articles formed therefrom |
-
1999
- 1999-08-27 US US09/384,960 patent/US6271360B1/en not_active Expired - Lifetime
-
2000
- 2000-08-25 AU AU70767/00A patent/AU773242C/en not_active Expired
- 2000-08-25 DK DK00959442.5T patent/DK1210123T3/en active
- 2000-08-25 PT PT100086024T patent/PT2266627E/en unknown
- 2000-08-25 CA CA2382120A patent/CA2382120C/en not_active Expired - Lifetime
- 2000-08-25 PT PT00959442T patent/PT1210123E/en unknown
- 2000-08-25 BR BR0013590-9A patent/BR0013590A/en not_active Application Discontinuation
- 2000-08-25 WO PCT/US2000/023457 patent/WO2001015740A1/en not_active Ceased
- 2000-08-25 US US09/645,889 patent/US6479292B1/en not_active Expired - Lifetime
- 2000-08-25 ES ES00959442T patent/ES2358292T3/en not_active Expired - Lifetime
- 2000-08-25 AT AT00959442T patent/ATE478687T1/en active
- 2000-08-25 DK DK10008602.4T patent/DK2266627T3/en active
- 2000-08-25 EP EP20100008602 patent/EP2266627B1/en not_active Expired - Lifetime
- 2000-08-25 EP EP20100183960 patent/EP2324856B1/en not_active Expired - Lifetime
- 2000-08-25 DE DE60044876T patent/DE60044876D1/en not_active Expired - Lifetime
- 2000-08-25 NZ NZ517942A patent/NZ517942A/en not_active IP Right Cessation
- 2000-08-25 ES ES10008602.4T patent/ES2542530T3/en not_active Expired - Lifetime
- 2000-08-25 EP EP00959442A patent/EP1210123B1/en not_active Expired - Lifetime
- 2000-08-25 CN CN008149968A patent/CN1384759B/en not_active Expired - Lifetime
- 2000-08-25 ES ES10183960.3T patent/ES2544727T3/en not_active Expired - Lifetime
- 2000-08-25 PT PT101839603T patent/PT2324856E/en unknown
- 2000-08-25 DK DK10183960.3T patent/DK2324856T3/en active
- 2000-08-25 JP JP2001520151A patent/JP5697187B2/en not_active Expired - Lifetime
-
2002
- 2002-02-13 ZA ZA200201228A patent/ZA200201228B/en unknown
- 2002-11-12 US US10/292,856 patent/US7060500B2/en not_active Expired - Lifetime
-
2004
- 2004-08-19 AU AU2004205139A patent/AU2004205139B2/en not_active Expired
-
2012
- 2012-05-10 JP JP2012108528A patent/JP5813571B2/en not_active Expired - Lifetime
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