ATE478947T1 - Kontinuierliche in vitro-evolution. - Google Patents

Kontinuierliche in vitro-evolution.

Info

Publication number
ATE478947T1
ATE478947T1 AT99918964T AT99918964T ATE478947T1 AT E478947 T1 ATE478947 T1 AT E478947T1 AT 99918964 T AT99918964 T AT 99918964T AT 99918964 T AT99918964 T AT 99918964T AT E478947 T1 ATE478947 T1 AT E478947T1
Authority
AT
Austria
Prior art keywords
mutant
mrna
population
protein
rna
Prior art date
Application number
AT99918964T
Other languages
English (en)
Inventor
Gregory Coia
Peter Hudson
Peter Iliades
Robert Irving
Original Assignee
Diatech Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diatech Pty Ltd filed Critical Diatech Pty Ltd
Application granted granted Critical
Publication of ATE478947T1 publication Critical patent/ATE478947T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—DNA viruses
    • C07K16/082—Hepadnaviridae (F), e.g. hepatitis B virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Superconductors And Manufacturing Methods Therefor (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
AT99918964T 1998-05-08 1999-05-07 Kontinuierliche in vitro-evolution. ATE478947T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPP3445A AUPP344598A0 (en) 1998-05-08 1998-05-08 Continuous one step in-vitro evolution
PCT/AU1999/000341 WO1999058661A1 (en) 1998-05-08 1999-05-07 Continuous in vitro evolution

Publications (1)

Publication Number Publication Date
ATE478947T1 true ATE478947T1 (de) 2010-09-15

Family

ID=3807699

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99918964T ATE478947T1 (de) 1998-05-08 1999-05-07 Kontinuierliche in vitro-evolution.

Country Status (9)

Country Link
US (1) US6562622B1 (de)
EP (1) EP1075513B8 (de)
JP (2) JP4519318B2 (de)
AT (1) ATE478947T1 (de)
AU (1) AUPP344598A0 (de)
CA (1) CA2328515C (de)
DE (1) DE69942701D1 (de)
ES (1) ES2351275T3 (de)
WO (1) WO1999058661A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030170820A1 (en) * 1998-05-08 2003-09-11 Gregory Coia Continuous in- vitro evolution
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
AU4854999A (en) * 1998-07-02 2000-01-24 Invitro Diagnostics, Inc. Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule
IL150291A0 (en) * 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
CA2402452A1 (en) * 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display
EP1652919A1 (de) * 2000-03-31 2006-05-03 Cambridge Antibody Technology LTD Verbesserungen in Bezug auf Ribosom-Komplexe als Selektionspartikel
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
AU2002359648A1 (en) * 2001-12-07 2003-06-23 Dyax Corporation Method and apparatus for washing magnetically responsive particles
DK1504098T4 (da) 2002-05-17 2011-05-23 Alligator Bioscience Ab Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
US8557743B2 (en) * 2002-09-05 2013-10-15 Dyax Corp. Display library process
WO2004039995A1 (en) * 2002-11-01 2004-05-13 Evogenix Pty Ltd Mutagenesis methods using ribavirin and/or rna replicases
BRPI0519008A2 (pt) 2004-12-13 2008-12-23 Evogenix Ltd proteÍnas variantes de osteoprotegerina
DE102005037349A1 (de) * 2005-08-08 2007-02-15 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vivo
US7879975B2 (en) * 2006-06-29 2011-02-01 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7777002B2 (en) * 2006-06-29 2010-08-17 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7923533B2 (en) * 2006-06-29 2011-04-12 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7993873B2 (en) * 2006-06-29 2011-08-09 The Invention Science Fund I, Llc Apparatus for arbitrary peptide synthesis
US7910695B2 (en) * 2006-06-29 2011-03-22 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
WO2008114011A2 (en) * 2007-03-19 2008-09-25 Medimmune Limited Fc polypeptide variants obtained by ribosome display methodology
CN101458255B (zh) * 2007-12-14 2013-03-06 武汉大学 丙型肝炎病毒包膜抗原夹心法试剂盒及检测方法
WO2012009760A1 (en) 2010-07-20 2012-01-26 Cephalon Australia Pty Ltd Anti-il-23 heterodimer specific antibodies
KR101982899B1 (ko) 2011-09-30 2019-05-27 테바 파마슈티컬즈 오스트레일리아 피티와이 엘티디 ＴＬ1ａ에 대한 항체 및 그의 용도
WO2014117220A1 (en) 2013-02-01 2014-08-07 Transbio Ltd Anti-cd83 antibodies and use thereof
CN117843785A (zh) 2013-02-07 2024-04-09 Csl有限公司 Il-11r结合蛋白及其应用
EP3209695A4 (de) 2014-10-23 2018-05-30 DendroCyte BioTech Pty Ltd Cd83-bindende proteine und verwendungen davon
WO2016094962A1 (en) 2014-12-19 2016-06-23 Monash University Il-21 antibodies
AU2016361462B2 (en) 2015-11-27 2023-09-21 Csl Limited CD131 binding proteins and uses thereof
EP4051714A4 (de) 2019-10-28 2023-11-22 Monash University Antikörper zum binden von plasmin
WO2021119761A1 (en) 2019-12-20 2021-06-24 Hudson Institute of Medical Research Cxcl10 binding proteins and uses thereof
JP7807019B2 (ja) * 2021-06-12 2026-01-27 リン シー‐ラン 新規なレプリカーゼサイクリング反応（ｒｃｒ）

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4786600A (en) 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
AU638762B2 (en) * 1989-10-05 1993-07-08 Optein Inc Cell-free synthesis and isolation of novel genes and polypeptides
WO1994006928A1 (en) 1992-09-24 1994-03-31 The Public Health Research Institute Of The City Of New York, Inc. Coupled replication-translation methods and kits for protein synthesis

Also Published As

Publication number Publication date
AUPP344598A0 (en) 1998-06-04
JP2010119396A (ja) 2010-06-03
EP1075513A1 (de) 2001-02-14
WO1999058661A1 (en) 1999-11-18
CA2328515A1 (en) 1999-11-18
EP1075513B1 (de) 2010-08-25
JP4519318B2 (ja) 2010-08-04
DE69942701D1 (de) 2010-10-07
EP1075513A4 (de) 2003-07-16
JP2002515224A (ja) 2002-05-28
EP1075513B8 (de) 2010-09-29
ES2351275T3 (es) 2011-02-02
US6562622B1 (en) 2003-05-13
CA2328515C (en) 2011-10-18

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