ATE480549T1 - Neues verfahren für die bisulfitbehandlung - Google Patents
Neues verfahren für die bisulfitbehandlungInfo
- Publication number
- ATE480549T1 ATE480549T1 AT06829518T AT06829518T ATE480549T1 AT E480549 T1 ATE480549 T1 AT E480549T1 AT 06829518 T AT06829518 T AT 06829518T AT 06829518 T AT06829518 T AT 06829518T AT E480549 T1 ATE480549 T1 AT E480549T1
- Authority
- AT
- Austria
- Prior art keywords
- new process
- bisulfite treatment
- nucleic acid
- bisulfite
- solid phase
- Prior art date
Links
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 239000007790 solid phase Substances 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 230000006326 desulfonation Effects 0.000 abstract 1
- 238000005869 desulfonation reaction Methods 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05027329 | 2005-12-14 | ||
| PCT/EP2006/011932 WO2007068437A1 (en) | 2005-12-14 | 2006-12-12 | New method for bisulfite treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE480549T1 true ATE480549T1 (de) | 2010-09-15 |
Family
ID=35539298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06829518T ATE480549T1 (de) | 2005-12-14 | 2006-12-12 | Neues verfahren für die bisulfitbehandlung |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8137937B2 (de) |
| EP (1) | EP1963352B1 (de) |
| JP (1) | JP5374679B2 (de) |
| CN (1) | CN101331148B (de) |
| AT (1) | ATE480549T1 (de) |
| CA (1) | CA2631829C (de) |
| DE (1) | DE602006016859D1 (de) |
| WO (1) | WO2007068437A1 (de) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003236461B2 (en) * | 2002-08-29 | 2009-05-28 | Epigenomics Ag | Improved method for bisulfite treatment |
| JP2008136404A (ja) * | 2006-11-30 | 2008-06-19 | Sysmex Corp | Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法 |
| JP2011505153A (ja) * | 2007-12-05 | 2011-02-24 | ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド | Ｒｎａのバイサルファイト処理 |
| GB0814570D0 (en) * | 2008-08-08 | 2008-09-17 | Diagnostics For The Real World | Isolation of nucleic acid |
| WO2010068289A2 (en) | 2008-12-11 | 2010-06-17 | Pacific Biosciences Of California, Inc. | Classification of nucleic acid templates |
| US9175338B2 (en) | 2008-12-11 | 2015-11-03 | Pacific Biosciences Of California, Inc. | Methods for identifying nucleic acid modifications |
| US12527665B2 (en) | 2008-12-11 | 2026-01-20 | Pacific Biosciences Of California, Inc. | Methods for identifying modified bases in nucleic acid templates |
| KR101912488B1 (ko) | 2011-09-07 | 2018-10-26 | 휴먼 제네틱 시그너처스 피티와이 엘티디 | 분자 검출 분석법 |
| ES2675584T3 (es) * | 2012-01-30 | 2018-07-11 | Exact Sciences Development Company, Llc | Modificación de ADN en perlas magnéticas |
| US9175348B2 (en) | 2012-04-24 | 2015-11-03 | Pacific Biosciences Of California, Inc. | Identification of 5-methyl-C in nucleic acid templates |
| ES2865124T3 (es) * | 2013-03-15 | 2021-10-15 | Abbott Molecular Inc | Procedimiento en una etapa para la purificación de ácidos nuncleicos |
| US20170253869A1 (en) * | 2014-10-17 | 2017-09-07 | Biochain Institute Inc. | Automated isolation and chemical reaction(s) of nucleic acids |
| US20160108459A1 (en) * | 2014-10-17 | 2016-04-21 | Biochain Institute Inc. | Automated isolation and chemical reaction(s) of nucleic acids |
| CA2981940A1 (en) | 2015-06-10 | 2016-12-15 | Biocartis N.V. | Improved detection of methylated dna |
| CN107922941A (zh) * | 2015-06-15 | 2018-04-17 | 塞弗德公司 | 在自动化反应盒中DNA甲基化的整合的纯化和测量以及突变和/或mRNA表达水平的共同测量 |
| CA3046636A1 (en) * | 2016-12-12 | 2018-06-21 | Cepheid | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
| CN117504806A (zh) * | 2017-10-31 | 2024-02-06 | 豪夫迈·罗氏有限公司 | 改进的磁性粒子及其用途 |
| CN115261467B (zh) * | 2017-12-01 | 2026-03-31 | 博尔诚（北京）科技有限公司 | 用于检测食道癌的组合物及其用途 |
| CN108265050A (zh) * | 2018-03-13 | 2018-07-10 | 普迈德（北京）科技有限公司 | 一种血浆直接亚硫酸氢盐转化的方法及其应用 |
| AU2019249422B2 (en) | 2018-04-02 | 2025-09-25 | Grail, Inc. | Methylation markers and targeted methylation probe panels |
| IL281741B2 (en) | 2018-09-27 | 2026-04-01 | Grail Inc | Methylation markers and a targeted methylation detection panel |
| JP7853277B2 (ja) * | 2020-07-29 | 2026-04-28 | エグザクト サイエンシーズ コーポレーション | スルホン化ｄｎａの精製 |
| CN116064733B (zh) * | 2023-01-18 | 2024-07-19 | 昂凯生命科技(苏州)有限公司 | 用于甲基化检测的样本前处理试剂盒及前处理方法 |
| WO2025250766A1 (en) * | 2024-05-29 | 2025-12-04 | The University Of Chicago | Methods and compositions for rapid detection and analysis of rna and dna modifications |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| ATE428801T1 (de) * | 1996-06-04 | 2009-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| DE10029915B4 (de) | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| DE10050942B4 (de) | 2000-10-10 | 2005-11-17 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| US20030152950A1 (en) * | 2001-06-27 | 2003-08-14 | Garner Harold R. | Identification of chemically modified polymers |
| AU2003236461B2 (en) * | 2002-08-29 | 2009-05-28 | Epigenomics Ag | Improved method for bisulfite treatment |
| ATE373673T1 (de) | 2003-01-29 | 2007-10-15 | Hoffmann La Roche | Verbessertes verfahren zur behandlung durch bisulfit |
| US7288373B2 (en) * | 2003-05-02 | 2007-10-30 | Human Genetic Signatures Pty Ltd. | Treatment of methylated nucleic acid |
| WO2005054502A1 (en) * | 2003-12-02 | 2005-06-16 | Roche Diagnostics Gmbh | Improved method for bisulfite treatment |
-
2006
- 2006-12-12 CN CN2006800472970A patent/CN101331148B/zh not_active Expired - Fee Related
- 2006-12-12 US US12/095,299 patent/US8137937B2/en active Active
- 2006-12-12 JP JP2008544851A patent/JP5374679B2/ja not_active Expired - Fee Related
- 2006-12-12 AT AT06829518T patent/ATE480549T1/de not_active IP Right Cessation
- 2006-12-12 CA CA2631829A patent/CA2631829C/en not_active Expired - Fee Related
- 2006-12-12 EP EP06829518A patent/EP1963352B1/de active Active
- 2006-12-12 WO PCT/EP2006/011932 patent/WO2007068437A1/en not_active Ceased
- 2006-12-12 DE DE602006016859T patent/DE602006016859D1/de active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2631829C (en) | 2012-01-24 |
| WO2007068437A1 (en) | 2007-06-21 |
| CN101331148A (zh) | 2008-12-24 |
| JP5374679B2 (ja) | 2013-12-25 |
| DE602006016859D1 (de) | 2010-10-21 |
| CA2631829A1 (en) | 2007-06-21 |
| US20080281087A1 (en) | 2008-11-13 |
| JP2009519023A (ja) | 2009-05-14 |
| US8137937B2 (en) | 2012-03-20 |
| EP1963352A1 (de) | 2008-09-03 |
| EP1963352B1 (de) | 2010-09-08 |
| CN101331148B (zh) | 2012-06-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |