ATE480618T1 - METHOD FOR COLLECTING AND USING NUCLEAR MRNA - Google Patents

METHOD FOR COLLECTING AND USING NUCLEAR MRNA

Info

Publication number
ATE480618T1
ATE480618T1 AT01270141T AT01270141T ATE480618T1 AT E480618 T1 ATE480618 T1 AT E480618T1 AT 01270141 T AT01270141 T AT 01270141T AT 01270141 T AT01270141 T AT 01270141T AT E480618 T1 ATE480618 T1 AT E480618T1
Authority
AT
Austria
Prior art keywords
mrna
cell
collecting
solution
novel method
Prior art date
Application number
AT01270141T
Other languages
German (de)
Inventor
Masato Mitsuhashi
Original Assignee
Hitachi Chemical Res Ct Inc
Hitachi Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Res Ct Inc, Hitachi Chemical Co Ltd filed Critical Hitachi Chemical Res Ct Inc
Application granted granted Critical
Publication of ATE480618T1 publication Critical patent/ATE480618T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention is related to a novel method of detecting mRNA, based on the fact that the newly synthesized mRNA is confined inside the cell nucleus. According to the novel method cells are captured on a membrane and then treated by a cell membrane permeation solution, thereby increasing permeability of cell membranes. After the cytoplasm is washed away, nuclei are dissolved with a cell dissolving solution, and then mRNA can be successfully recovered from the obtained solution.
AT01270141T 2000-10-31 2001-10-25 METHOD FOR COLLECTING AND USING NUCLEAR MRNA ATE480618T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24467200P 2000-10-31 2000-10-31
US30803801P 2001-07-26 2001-07-26
PCT/US2001/049498 WO2002066637A2 (en) 2000-10-31 2001-10-25 Method for collecting and using nuclear mrna

Publications (1)

Publication Number Publication Date
ATE480618T1 true ATE480618T1 (en) 2010-09-15

Family

ID=26936712

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01270141T ATE480618T1 (en) 2000-10-31 2001-10-25 METHOD FOR COLLECTING AND USING NUCLEAR MRNA

Country Status (7)

Country Link
US (1) US7374881B2 (en)
EP (1) EP1330518B1 (en)
JP (1) JP5416326B2 (en)
CN (1) CN1294262C (en)
AT (1) ATE480618T1 (en)
DE (1) DE60143046D1 (en)
WO (1) WO2002066637A2 (en)

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ATE512234T1 (en) * 2005-04-28 2011-06-15 Hitachi Chemical Res Ct Inc EX-VIVO GENE EXPRESSION IN WHOLE BLOOD AS A MODEL FOR ASSESSING INDIVIDUAL VARIATION AGAINST FOOD ADDITIVES
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ATE518011T1 (en) * 2006-04-07 2011-08-15 Hitachi Chemical Co Ltd INCREASED EXPRESSION OF T-CELL RECEPTOR-MEDIATED TUMOR NECROSIS FACTOR SUPERFAMILY AND CHEMOKINE MRNA IN PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH CROHN'S DISEASE
JP2009536021A (en) * 2006-04-07 2009-10-08 日立化成工業株式会社 Increased FC receptor-mediated tumor necrosis factor superfamily and chemokine mRNA expression in peripheral blood leukocytes of patients with rheumatoid arthritis
JP2011502535A (en) 2007-11-14 2011-01-27 日立化成工業株式会社 Tumor necrosis factor superfamily mRNA expression through Fc receptors in peripheral blood leukocytes
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
US8304185B2 (en) 2009-07-17 2012-11-06 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
US20100062946A1 (en) * 2008-09-08 2010-03-11 Lis John T Genome-wide method for mapping of engaged rna polymerases quantitatively and at high resolution
CN101497925B (en) * 2008-12-17 2012-05-02 安徽医科大学 Leber's hereditary optic neuropathy-related mtDNA mutation site integrated detection gene chip and its preparation and application
JP5729904B2 (en) * 2009-06-02 2015-06-03 キヤノン株式会社 Methods for preparing proteins, DNA, RNA from cells
EP2454378B1 (en) * 2009-07-17 2015-12-23 Canon U.S. Life Sciences, Inc. Methods and systems for dna isolation on a microfluidic device
MX2012001236A (en) * 2009-08-04 2012-03-16 Du Pont Process and device for collecting nucleic acids of microorganisms from a particulate sample.
WO2011068088A1 (en) 2009-12-04 2011-06-09 株式会社日立製作所 GENE EXPRESSION ANALYSIS METHOD USING TWO DIMENSIONAL cDNA LIBRARY
WO2011156763A1 (en) 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Methods for characterizing kidney function
WO2011156734A2 (en) * 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
US20110312537A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Loc device for amplifying and detecting target nucleic acid sequences using electrochemiluminescent resonant energy transfer, linear probes with covalently attached primers
JP5823031B2 (en) 2011-06-10 2015-11-25 日立化成株式会社 Vesicle capture device and method for using the same
WO2014182330A1 (en) 2013-05-06 2014-11-13 Hitachi Chemical Company Ltd Devices and methods for capturing target molecules
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
EP3218521B1 (en) 2014-11-12 2019-12-25 Hitachi Chemical Co., Ltd. Method for diagnosing organ injury
DE112016003948T5 (en) 2015-08-31 2018-05-09 City Of Sapporo MOLECULAR METHODS FOR EVALUATING A UROTHIAL DISEASE
US11293049B2 (en) * 2016-01-08 2022-04-05 Pathoquest Modulation of accessibility of host nucleic acids to nucleic acid digesting enzymes in acellular biological fluids
CN115247204A (en) * 2022-02-15 2022-10-28 温州医科大学 A method for detecting subcellular localization of lncRNA based on qRT-PCR technology
CN115508269A (en) * 2022-10-20 2022-12-23 上海华简检测技术有限公司 Quantitative sampling contrast analysis method for cell treating agent

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Also Published As

Publication number Publication date
EP1330518A2 (en) 2003-07-30
US7374881B2 (en) 2008-05-20
EP1330518B1 (en) 2010-09-08
JP2004519234A (en) 2004-07-02
WO2002066637A2 (en) 2002-08-29
DE60143046D1 (en) 2010-10-21
JP5416326B2 (en) 2014-02-12
CN1606621A (en) 2005-04-13
WO2002066637A3 (en) 2003-05-22
CN1294262C (en) 2007-01-10
WO2002066637A8 (en) 2003-10-30
US20040072193A1 (en) 2004-04-15

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