ATE481499T1 - Cocktail von nukleaseinhibitoren - Google Patents

Cocktail von nukleaseinhibitoren

Info

Publication number
ATE481499T1
ATE481499T1 AT00966924T AT00966924T ATE481499T1 AT E481499 T1 ATE481499 T1 AT E481499T1 AT 00966924 T AT00966924 T AT 00966924T AT 00966924 T AT00966924 T AT 00966924T AT E481499 T1 ATE481499 T1 AT E481499T1
Authority
AT
Austria
Prior art keywords
nuclease
antibodies
inhibitors
antibody
nuclease inhibitors
Prior art date
Application number
AT00966924T
Other languages
English (en)
Inventor
Matthew Winkler
W Kudlicki
Brittan Pasloske
Original Assignee
Ambion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc filed Critical Ambion Inc
Application granted granted Critical
Publication of ATE481499T1 publication Critical patent/ATE481499T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/916Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Alcoholic Beverages (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Peptides Or Proteins (AREA)
AT00966924T 1999-09-24 2000-09-25 Cocktail von nukleaseinhibitoren ATE481499T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15587499P 1999-09-24 1999-09-24
PCT/US2000/026485 WO2001021830A1 (en) 1999-09-24 2000-09-25 Nuclease inhibitor cocktail

Publications (1)

Publication Number Publication Date
ATE481499T1 true ATE481499T1 (de) 2010-10-15

Family

ID=22557124

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00966924T ATE481499T1 (de) 1999-09-24 2000-09-25 Cocktail von nukleaseinhibitoren

Country Status (5)

Country Link
US (3) US6664379B1 (de)
EP (1) EP1220944B1 (de)
AT (1) ATE481499T1 (de)
DE (1) DE60044977D1 (de)
WO (1) WO2001021830A1 (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
ATE481499T1 (de) * 1999-09-24 2010-10-15 Ambion Inc Cocktail von nukleaseinhibitoren
US7264932B2 (en) * 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
JP4328530B2 (ja) * 2001-03-08 2009-09-09 ライフ テクノロジーズ コーポレーション 改良インビトロ合成系
EP1300465A1 (de) * 2001-10-08 2003-04-09 Agrobiogen GmbH Biotechnologie Verfahren und Vorrichtung zur Isolierung von RNS-Proben
EP2163652B2 (de) 2002-01-28 2023-05-31 Life Technologies Corporation Herstellung biologische Rohderivaten unter Einzetsung Proteasen, geeignet für cDNA Synthese
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
ATE447040T1 (de) 2002-09-03 2009-11-15 Quanta Biosciences Inc Verbesserte zusammensetzungen und verfahren für die cdna-synthese
CA2445204C (en) 2002-10-16 2014-08-12 Streck Laboratories, Inc. Method and device for collecting and preserving cells for analysis
US7291459B2 (en) * 2002-12-10 2007-11-06 University Of Alabama At Huntsville Nucleic acid detector and method of detecting targets within a sample
US20070032418A1 (en) * 2003-02-25 2007-02-08 Ambion, Inc Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same
JP4871722B2 (ja) 2003-07-25 2012-02-08 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 固定試料からrnaを調製するための方法および組成物
US20050208501A1 (en) 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US8460684B2 (en) 2004-03-19 2013-06-11 Wisconsin Alumni Research Foundation Nuclease inhibitors and methods for their use
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20080004436A1 (en) * 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
DE102005015005A1 (de) * 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
WO2008040126A1 (en) 2006-10-06 2008-04-10 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
CN102265152A (zh) * 2008-10-06 2011-11-30 西福根有限公司 鉴定和选择用于组合药品的药物候选物
EP2175020A1 (de) * 2008-10-13 2010-04-14 Roche Diagnostics GmbH Verringerung der RNase-Aktivität in komplexen flüssigen Proben
EA201100610A1 (ru) * 2008-10-30 2011-10-31 Йиссум Ресёрч Девелопмент Компани Оф Дзе Хебрю Юниверсити Оф Иерусалим Лтд. Эффективная экспрессия укороченного человеческого белка rnaset2 в e.coli
EP2955233A1 (de) 2008-12-19 2015-12-16 Life Technologies Corporation Proteinase K Inhibitore, Verfahren und Zusammensetzungen davon
US11634747B2 (en) * 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
DK2398912T3 (en) 2009-02-18 2017-10-30 Streck Inc Conservation of cell-free nucleic acids
US20110027862A1 (en) * 2009-06-29 2011-02-03 Life Technologies Corporation Sample stabilization
ES2571104T3 (es) 2009-11-09 2016-05-24 Streck Inc Estabilización del ARN y extracción del ARN presente en células intactas dentro de una muestra de sangre
CN102243154B (zh) * 2011-04-02 2016-05-11 中山大学附属第一医院 一种周围神经切片染色的方法
US9956281B2 (en) 2011-05-04 2018-05-01 Streck, Inc. Inactivated virus compositions and methods of preparing such compositions
KR20140040239A (ko) 2011-06-19 2014-04-02 아보젠, 인크. 샘플 수집을 위한 장치, 용액 및 방법
WO2013055898A1 (en) * 2011-10-14 2013-04-18 The Trustees Of The University Of Pennsylvania Methods for enhancing the efficacy of microrna mediated interference
US9604213B2 (en) 2012-02-13 2017-03-28 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9738887B2 (en) 2012-02-13 2017-08-22 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
US9637775B2 (en) 2012-02-13 2017-05-02 Neumodx Molecular, Inc. System and method for processing biological samples
US11485968B2 (en) 2012-02-13 2022-11-01 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
US11648561B2 (en) 2012-02-13 2023-05-16 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
JP6236864B2 (ja) * 2012-06-11 2017-11-29 東ソー株式会社 癌の検出方法及び膵臓特異的リボヌクレアーゼ1を認識する抗体
AU2013318156A1 (en) * 2012-09-19 2015-03-19 Beckman Coulter, Inc. Use of divalent ions, proteases, detergents, and low pH in the extraction of nucleic acids
ES2741749T3 (es) 2012-10-25 2020-02-12 Neumodx Molecular Inc Método y materiales para el aislamiento de materiales de ácido nucleico
ES2938048T3 (es) 2013-07-24 2023-04-04 Streck Llc Composiciones y procedimientos para estabilizar las células tumorales circulantes
EP3680348B1 (de) 2014-02-07 2023-04-12 University of Iowa Research Foundation Oligonukleotidbasierte sonden und verfahren zum nachweis von mikroben
AU2015277059B2 (en) 2014-06-18 2021-06-17 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
EP4035762B1 (de) 2015-09-09 2023-11-01 Drawbridge Health, Inc. Vorrichtungen zur probeentnahme, stabilisierung und konservierung
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
EP3389481A4 (de) 2015-12-18 2019-05-22 Clear Gene, Inc. Verfahren, zusammensetzungen, kits und vorrichtungen zur schnellanalyse von biologischen markern
WO2018022991A1 (en) 2016-07-29 2018-02-01 Streck, Inc. Suspension composition for hematology analysis control
US12275994B2 (en) 2017-06-22 2025-04-15 Clear Gene, Inc. Methods and compositions for the analysis of cancer biomarkers
WO2019067711A1 (en) 2017-09-29 2019-04-04 University Of Iowa Research Foundation DIGITAL NUCLEASE DETECTION COMPOSITIONS AND METHODS
US12378543B2 (en) 2017-10-19 2025-08-05 Streck Llc Compositions for hemolysis and coagulation regulation and stabilization of extracellular vesicles
US20230272465A1 (en) * 2020-07-13 2023-08-31 The Board Of Trustees Of The Leland Stanford Junior University Systems and Methods to Enhance RNA Transcription and Uses Thereof
CN114249829B (zh) * 2021-12-20 2022-09-20 南京诺唯赞生物科技股份有限公司 一种RNase抑制剂的单克隆抗体及其制备方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58116498A (ja) * 1981-12-28 1983-07-11 Takeda Chem Ind Ltd Il‐2をコードする新規伝令rnaの製造法
US5278062A (en) * 1987-04-03 1994-01-11 Amgen Proteolytic enzymes
US5151504A (en) * 1989-11-17 1992-09-29 E. R. Squibb & Sons, Inc. Method for purification of monoclonal antibodies
US5470971A (en) * 1991-03-11 1995-11-28 The University Of Medicine And Dentistry Of New Jersey Stress-induced proteins, genes coding therefor, transformed cells of organisms, methods and applications
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US6110968A (en) * 1995-12-26 2000-08-29 The Picower Institute For Medical Research Methods for treatment predicated on the presence of advanced glycosylation endproducts in tobacco and its combustion byproducts
US5973137A (en) * 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
JPH11508613A (ja) 1996-05-02 1999-07-27 ザ、プロクター、エンド、ギャンブル、カンパニー 分散した界面活性剤錯体を含む局所用組成物
ES2198570T3 (es) 1996-05-02 2004-02-01 THE PROCTER & GAMBLE COMPANY Composiciones para uso topico que comprenden un complejo tensioactivo dispersado.
US5968784A (en) * 1997-01-15 1999-10-19 Chugai Pharmaceutical Co., Ltd. Method for analyzing quantitative expression of genes
US5972613A (en) * 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
US6869604B1 (en) * 1998-03-27 2005-03-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Recombinant anti-tumor RNAse
US7264932B2 (en) * 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
ATE481499T1 (de) * 1999-09-24 2010-10-15 Ambion Inc Cocktail von nukleaseinhibitoren
US6793019B2 (en) 2002-07-10 2004-09-21 Abb Offshore Systems, Inc. Tapered ramp positive lock latch mechanism
US7021540B2 (en) * 2002-07-30 2006-04-04 Canon Kabushiki Kaisha Storage medium mounting/dismounting mechanism and information processing apparatus
US20070032418A1 (en) * 2003-02-25 2007-02-08 Ambion, Inc Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same

Also Published As

Publication number Publication date
EP1220944A4 (de) 2005-06-22
EP1220944B1 (de) 2010-09-15
US20070148174A1 (en) 2007-06-28
WO2001021830A1 (en) 2001-03-29
DE60044977D1 (de) 2010-10-28
US20050026284A1 (en) 2005-02-03
US7163793B2 (en) 2007-01-16
US7445901B2 (en) 2008-11-04
EP1220944A1 (de) 2002-07-10
US6664379B1 (en) 2003-12-16

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