ATE483802T1 - Verfahren zur in vitro-rekombination - Google Patents
Verfahren zur in vitro-rekombinationInfo
- Publication number
- ATE483802T1 ATE483802T1 AT06836102T AT06836102T ATE483802T1 AT E483802 T1 ATE483802 T1 AT E483802T1 AT 06836102 T AT06836102 T AT 06836102T AT 06836102 T AT06836102 T AT 06836102T AT E483802 T1 ATE483802 T1 AT E483802T1
- Authority
- AT
- Austria
- Prior art keywords
- molecules
- dna
- region
- stranded
- substantially purified
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000000338 in vitro Methods 0.000 title abstract 2
- 238000005215 recombination Methods 0.000 title 1
- 230000006798 recombination Effects 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 9
- 102000053602 DNA Human genes 0.000 abstract 7
- 108060002716 Exonuclease Proteins 0.000 abstract 2
- 102000003960 Ligases Human genes 0.000 abstract 2
- 108090000364 Ligases Proteins 0.000 abstract 2
- 102000013165 exonuclease Human genes 0.000 abstract 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Battery Electrode And Active Subsutance (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70717705P | 2005-08-11 | 2005-08-11 | |
| US80040006P | 2006-05-16 | 2006-05-16 | |
| PCT/US2006/031214 WO2007032837A2 (en) | 2005-08-11 | 2006-08-11 | Method for in vitro recombination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE483802T1 true ATE483802T1 (de) | 2010-10-15 |
Family
ID=37734039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06836102T ATE483802T1 (de) | 2005-08-11 | 2006-08-11 | Verfahren zur in vitro-rekombination |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US7776532B2 (de) |
| EP (2) | EP2239327B1 (de) |
| AT (1) | ATE483802T1 (de) |
| CA (1) | CA2618665C (de) |
| DE (1) | DE602006017405D1 (de) |
| DK (2) | DK2239327T3 (de) |
| MY (1) | MY144014A (de) |
| WO (1) | WO2007032837A2 (de) |
Families Citing this family (66)
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|---|---|---|---|---|
| DK1915446T3 (en) | 2005-08-11 | 2017-09-11 | Synthetic Genomics Inc | IN VITRO RECOMBINATION PROCEDURE |
| WO2007028147A2 (en) * | 2005-09-01 | 2007-03-08 | Philadelphia Health & Education Corporation D.B.A. Drexel University College Of Medicin | Identification of a prostatic intraepithelial neoplasia (pin)-specific gene and protein (pin-1) useful as a diagnostic treatment for prostate cancer |
| US7696335B2 (en) * | 2005-10-13 | 2010-04-13 | Bc Cancer Agency | Kits for multiple non-cross reacting recombination reactions utilizing loxP sequences |
| EP1968994B1 (de) * | 2005-12-06 | 2013-07-03 | Synthetic Genomics, Inc. | Synthetische genome |
| US9315536B2 (en) * | 2006-08-29 | 2016-04-19 | Creatrone, Inc. | Self-assembling oligonucleotides and methods |
| US9593329B2 (en) | 2007-10-08 | 2017-03-14 | Synthetic Genomics, Inc. | Assembly of large nucleic acids |
| US9267132B2 (en) | 2007-10-08 | 2016-02-23 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| EP3064599B1 (de) * | 2008-02-15 | 2018-12-12 | Synthetic Genomics, Inc. | Verfahren zur in-vitro-verbindung und zur kombinatorischen anordnung von nukleinsäuremolekülen |
| US9259662B2 (en) | 2008-02-22 | 2016-02-16 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US10093552B2 (en) | 2008-02-22 | 2018-10-09 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US8735651B2 (en) | 2008-02-23 | 2014-05-27 | James Weifu Lee | Designer organisms for photobiological butanol production from carbon dioxide and water |
| US8986963B2 (en) | 2008-02-23 | 2015-03-24 | James Weifu Lee | Designer calvin-cycle-channeled production of butanol and related higher alcohols |
| EP2282769A4 (de) | 2008-04-29 | 2012-04-25 | Abbott Lab | Dual-variable- domain-immunglobuline und ihre verwendungen |
| EP2297209A4 (de) | 2008-06-03 | 2012-08-01 | Abbott Lab | Dual-variable- domain-immunglobuline und ihre verwendungen |
| WO2010030776A1 (en) * | 2008-09-10 | 2010-03-18 | Genscript Corporation | Homologous recombination-based dna cloning methods and compositions |
| WO2010045326A1 (en) * | 2008-10-14 | 2010-04-22 | Barnes Wayne M | In vitro recombination methods using 5' exonuclease |
| EP2358875B1 (de) * | 2008-11-19 | 2015-08-12 | Amyris, Inc. | Zusammensetzungen und verfahren zur konstruktion von polynukleotiden |
| EP3249045A1 (de) | 2009-03-06 | 2017-11-29 | Synthetic Genomics, Inc. | Verfahren zum klonen und manipulieren von genomen |
| CN102939380A (zh) | 2010-03-05 | 2013-02-20 | 合成基因组股份有限公司 | 用于克隆和操作基因组的方法 |
| UY33492A (es) | 2010-07-09 | 2012-01-31 | Abbott Lab | Inmunoglobulinas con dominio variable dual y usos de las mismas |
| KR20130100118A (ko) | 2010-08-03 | 2013-09-09 | 아비에 인코포레이티드 | 이원 가변 도메인 면역글로불린 및 이의 용도 |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
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| WO2013133132A1 (ja) * | 2012-03-06 | 2013-09-12 | タカラバイオ株式会社 | 二本鎖ｄｎａの製造方法 |
| JP6004423B2 (ja) * | 2012-05-07 | 2016-10-05 | 国立研究開発法人産業技術総合研究所 | 遺伝子連結法およびそれを用いた単鎖抗体作製方法 |
| KR102160389B1 (ko) | 2013-08-05 | 2020-09-28 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
| KR101398764B1 (ko) | 2013-08-29 | 2014-05-27 | 강릉원주대학교산학협력단 | 입자의 이동에 의해 분석물질을 검출하는 장치 및 방법 |
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| JP6594955B2 (ja) * | 2014-08-27 | 2019-10-23 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | シントン形成 |
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| CA2975852A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| CA2975855C (en) | 2015-02-04 | 2025-09-23 | Twist Bioscience Corporation | SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS |
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| KR20180050411A (ko) | 2015-09-18 | 2018-05-14 | 트위스트 바이오사이언스 코포레이션 | 올리고핵산 변이체 라이브러리 및 그의 합성 |
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| EP3384077A4 (de) | 2015-12-01 | 2019-05-08 | Twist Bioscience Corporation | Funktionalisierte oberflächen und herstellung davon |
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-
2006
- 2006-08-11 WO PCT/US2006/031214 patent/WO2007032837A2/en not_active Ceased
- 2006-08-11 US US11/502,624 patent/US7776532B2/en not_active Expired - Lifetime
- 2006-08-11 DK DK10168787.9T patent/DK2239327T3/en active
- 2006-08-11 CA CA2618665A patent/CA2618665C/en not_active Expired - Lifetime
- 2006-08-11 EP EP10168787.9A patent/EP2239327B1/de not_active Expired - Lifetime
- 2006-08-11 MY MYPI20063911A patent/MY144014A/en unknown
- 2006-08-11 EP EP06836102A patent/EP1929012B1/de not_active Expired - Lifetime
- 2006-08-11 DE DE602006017405T patent/DE602006017405D1/de not_active Expired - Lifetime
- 2006-08-11 DK DK06836102.1T patent/DK1929012T3/da active
- 2006-08-11 AT AT06836102T patent/ATE483802T1/de not_active IP Right Cessation
-
2010
- 2010-06-22 US US12/820,861 patent/US8435736B2/en active Active
-
2013
- 2013-04-16 US US13/864,108 patent/US20130295645A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8435736B2 (en) | 2013-05-07 |
| EP1929012B1 (de) | 2010-10-06 |
| DK1929012T3 (da) | 2011-01-31 |
| EP2239327A3 (de) | 2012-04-25 |
| US7776532B2 (en) | 2010-08-17 |
| EP2239327B1 (de) | 2015-02-25 |
| EP2239327A2 (de) | 2010-10-13 |
| CA2618665C (en) | 2012-11-13 |
| US20130295645A1 (en) | 2013-11-07 |
| US20070037196A1 (en) | 2007-02-15 |
| US20100311126A1 (en) | 2010-12-09 |
| CA2618665A1 (en) | 2007-03-22 |
| MY144014A (en) | 2011-07-29 |
| WO2007032837A2 (en) | 2007-03-22 |
| DE602006017405D1 (de) | 2010-11-18 |
| EP1929012A2 (de) | 2008-06-11 |
| WO2007032837A3 (en) | 2007-05-24 |
| DK2239327T3 (en) | 2015-05-18 |
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