ATE486944T1 - Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon - Google Patents
Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davonInfo
- Publication number
- ATE486944T1 ATE486944T1 AT06701176T AT06701176T ATE486944T1 AT E486944 T1 ATE486944 T1 AT E486944T1 AT 06701176 T AT06701176 T AT 06701176T AT 06701176 T AT06701176 T AT 06701176T AT E486944 T1 ATE486944 T1 AT E486944T1
- Authority
- AT
- Austria
- Prior art keywords
- promoter
- pichia pastoris
- selongation
- translation
- recombinant protein
- Prior art date
Links
- 241000235058 Komagataella pastoris Species 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 239000013604 expression vector Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000003259 recombinant expression Methods 0.000 abstract 2
- 102000002508 Peptide Elongation Factors Human genes 0.000 abstract 1
- 108010068204 Peptide Elongation Factors Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000000411 inducer Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20050109697 | 2005-11-16 | ||
| PCT/KR2006/000215 WO2007058407A1 (en) | 2005-11-16 | 2006-01-19 | Translational elongation factor promoter from pichia pastoris and method for producing recombinant protein using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE486944T1 true ATE486944T1 (de) | 2010-11-15 |
Family
ID=38048774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06701176T ATE486944T1 (de) | 2005-11-16 | 2006-01-19 | Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7816509B2 (de) |
| EP (1) | EP1951877B1 (de) |
| KR (1) | KR100752107B1 (de) |
| AT (1) | ATE486944T1 (de) |
| DE (1) | DE602006018054D1 (de) |
| WO (1) | WO2007058407A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100717353B1 (ko) * | 2006-04-12 | 2007-05-11 | 한국생명공학연구원 | 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법 |
| US8450270B2 (en) | 2008-06-17 | 2013-05-28 | Indiana University Research And Technology Corporation | Glucagon analogs exhibiting enhanced solubility and stability in physiological pH buffers |
| EP2258855A1 (de) | 2009-05-28 | 2010-12-08 | Universität für Bodenkultur Wien | Expressionssequenzen |
| EP2764016B1 (de) | 2011-10-07 | 2019-03-13 | Lonza Ltd | Regulierbarer promoter |
| HUE042897T2 (hu) * | 2013-03-08 | 2019-07-29 | Biogrammatics Inc | Élesztõpromóterek fehérje-expresszióhoz |
| US9150870B2 (en) | 2013-03-15 | 2015-10-06 | Lonza Ltd. | Constitutive promoter |
| DK2970994T3 (da) | 2013-03-15 | 2019-10-28 | Lonza Ag | Konstitutiv promotor |
| EP3824083A4 (de) | 2018-09-24 | 2022-01-05 | Orta Dogu Teknik Universitesi | Entwurf von alkoholdehydrogenase-2(adh2)-promotorvarianten durch promotor-engineering |
| EP3824084A4 (de) | 2018-09-24 | 2021-12-22 | Orta Dogu Teknik Universitesi | Modifizierte aox1-promotorvarianten |
| CN114657197B (zh) * | 2022-04-06 | 2023-07-21 | 暨南大学 | Gsm1p作为正调控因子在提高宿主细胞中蛋白表达中的应用 |
| CN114774461B (zh) * | 2022-04-06 | 2023-05-26 | 暨南大学 | Ash1p作为负调控因子在提高宿主细胞中蛋白表达中的应用 |
| CN118308360B (zh) * | 2024-06-07 | 2024-08-16 | 中国人民解放军军事科学院军事医学研究院 | 驱动基因在酵母中表达人血清白蛋白的启动子与应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5989870A (en) | 1986-04-30 | 1999-11-23 | Rohm Enzyme Finland Oy | Method for cloning active promoters |
| US5650294A (en) * | 1990-06-25 | 1997-07-22 | Basf Aktiengesellschaft | Isolated promoter and terminator of elongation factor 1-α |
| US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| WO1997044470A1 (en) | 1996-05-21 | 1997-11-27 | Novo Nordisk A/S | Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast |
| ID23301A (id) | 1998-04-23 | 2000-04-05 | Hoffmann La Roche | Produksi yang berlebihan dari riboflavin di dalam ragi |
| US20030064437A1 (en) * | 2000-11-15 | 2003-04-03 | Nick Wan | Expression system for recombinant proteins |
| KR100646100B1 (ko) | 2004-11-27 | 2006-11-15 | 한국생명공학연구원 | 미생물 유래의 신규 프로모터의 스크리닝 방법 |
-
2006
- 2006-01-19 US US12/094,138 patent/US7816509B2/en active Active
- 2006-01-19 DE DE602006018054T patent/DE602006018054D1/de not_active Expired - Lifetime
- 2006-01-19 WO PCT/KR2006/000215 patent/WO2007058407A1/en not_active Ceased
- 2006-01-19 EP EP06701176A patent/EP1951877B1/de not_active Expired - Lifetime
- 2006-01-19 AT AT06701176T patent/ATE486944T1/de not_active IP Right Cessation
- 2006-02-07 KR KR1020060011475A patent/KR100752107B1/ko not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7816509B2 (en) | 2010-10-19 |
| US20090298122A1 (en) | 2009-12-03 |
| EP1951877A1 (de) | 2008-08-06 |
| EP1951877B1 (de) | 2010-11-03 |
| KR100752107B1 (ko) | 2007-08-24 |
| EP1951877A4 (de) | 2009-02-18 |
| KR20070052179A (ko) | 2007-05-21 |
| WO2007058407A1 (en) | 2007-05-24 |
| DE602006018054D1 (de) | 2010-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |