ATE488587T1 - Rückübertragungs-primer und verfahren zu deren entwurf - Google Patents
Rückübertragungs-primer und verfahren zu deren entwurfInfo
- Publication number
- ATE488587T1 ATE488587T1 AT06846486T AT06846486T ATE488587T1 AT E488587 T1 ATE488587 T1 AT E488587T1 AT 06846486 T AT06846486 T AT 06846486T AT 06846486 T AT06846486 T AT 06846486T AT E488587 T1 ATE488587 T1 AT E488587T1
- Authority
- AT
- Austria
- Prior art keywords
- transcripts
- primers
- retransmission
- design
- oligonucleotides
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Medical Informatics (AREA)
- Evolutionary Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Theoretical Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Adhesives Or Adhesive Processes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74282705P | 2005-12-06 | 2005-12-06 | |
| PCT/US2006/061643 WO2007067907A1 (en) | 2005-12-06 | 2006-12-06 | Reverse transcription primers and methods of design |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE488587T1 true ATE488587T1 (de) | 2010-12-15 |
Family
ID=37903511
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06846486T ATE488587T1 (de) | 2005-12-06 | 2006-12-06 | Rückübertragungs-primer und verfahren zu deren entwurf |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US8268987B2 (de) |
| EP (2) | EP2206779B1 (de) |
| AT (1) | ATE488587T1 (de) |
| DE (1) | DE602006018352D1 (de) |
| WO (1) | WO2007067907A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007044091A2 (en) | 2005-06-02 | 2007-04-19 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
| DE602006018352D1 (de) | 2005-12-06 | 2010-12-30 | Ambion Inc | Rückübertragungs-primer und verfahren zu deren entwurf |
| SG183029A1 (en) | 2009-01-13 | 2012-08-30 | Fluidigm Corp | Single-cell nucleic acid analysis |
| WO2013177206A2 (en) | 2012-05-21 | 2013-11-28 | Fluidigm Corporation | Single-particle analysis of particle populations |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| CN111334504B (zh) * | 2020-03-04 | 2022-03-25 | 苏州系统医学研究所 | 一种核内病毒模拟物的递送系统、制备方法及应用 |
| CN114317529B (zh) * | 2021-12-13 | 2023-12-01 | 北京大学 | 一种寡核苷酸链随机拼合方法 |
Family Cites Families (68)
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| US2939701A (en) | 1955-09-28 | 1960-06-07 | Schroter Friedrich | Method of and means for feeding work pieces in the form of sheets |
| US5723597A (en) | 1983-01-10 | 1998-03-03 | Gen-Probe Incorporated | Ribosomal nucleic acid probes for detecting organisms or groups of organisms |
| US6221581B1 (en) | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
| US6150517A (en) | 1986-11-24 | 2000-11-21 | Gen-Probe | Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms |
| US5541308A (en) | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| US5525464A (en) * | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
| US5639604A (en) | 1987-09-21 | 1997-06-17 | Gen-Probe Incorporated | Homogeneous protection assay |
| US5084565A (en) | 1988-08-18 | 1992-01-28 | Gene-Trak Systems | Probes for the specific detection of escherichia coli and shigella |
| US5512439A (en) | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| US5708154A (en) | 1989-02-24 | 1998-01-13 | City Of Hope | RNA-DNA hybrid molecules of nucleic acid |
| WO1990010717A1 (en) | 1989-03-10 | 1990-09-20 | Gene-Trak Systems | Preventing interference with affinity capture schemes |
| CA2031499A1 (en) | 1989-05-31 | 1990-12-01 | David J. Lane | Universal eubacteria nucleic acid probes and methods |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| CA2025181A1 (en) | 1989-10-12 | 1991-04-13 | William G. Weisburg | Nucleic acid probes and methods for detecting fungi |
| EP0675966B1 (de) | 1992-02-19 | 2004-10-06 | The Public Health Research Institute Of The City Of New York, Inc. | Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren |
| KR100249110B1 (ko) | 1992-05-06 | 2000-04-01 | 다니엘 엘. 캐시앙 | 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트 |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5633134A (en) | 1992-10-06 | 1997-05-27 | Ig Laboratories, Inc. | Method for simultaneously detecting multiple mutations in a DNA sample |
| US5500356A (en) | 1993-08-10 | 1996-03-19 | Life Technologies, Inc. | Method of nucleic acid sequence selection |
| US6309823B1 (en) | 1993-10-26 | 2001-10-30 | Affymetrix, Inc. | Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same |
| US5639428A (en) | 1994-07-19 | 1997-06-17 | Becton Dickinson And Company | Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay |
| FR2737223B1 (fr) | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| FR2737502B1 (fr) | 1995-07-31 | 1997-10-24 | Genset Sa | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
| US6013440A (en) | 1996-03-11 | 2000-01-11 | Affymetrix, Inc. | Nucleic acid affinity columns |
| JPH11509427A (ja) | 1996-05-14 | 1999-08-24 | ベーリンガー マンハイム ゲーエムベーハー | 試料中の核酸分子の発現の同定方法および／または定量方法 |
| US6203978B1 (en) | 1996-05-31 | 2001-03-20 | Du Vergier Ltd. | Capture of single stranded nucleic acids |
| WO1998012354A1 (en) | 1996-09-19 | 1998-03-26 | Affymetrix, Inc. | Identification of molecular sequence signatures and methods involving the same |
| EP0972830A4 (de) | 1997-02-21 | 2003-02-05 | Takara Shuzo Co | Krebs-assoziierte gene |
| EP0975807B1 (de) | 1997-05-02 | 2006-09-27 | Gen-Probe Incorporated | Zwei-schritt hybridisierung und einfang von einem polynukleotid |
| EP1007739A2 (de) | 1997-09-05 | 2000-06-14 | Sidney Kimmel Cancer Center | Auswahl von pcr primerpaaren zur amplifikation einer gruppe von nukleotidsequenzen |
| JP4236812B2 (ja) | 1997-09-12 | 2009-03-11 | エクシコン エ／エス | オリゴヌクレオチド類似体 |
| US5932451A (en) | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| US6270973B1 (en) | 1998-03-13 | 2001-08-07 | Promega Corporation | Multiplex method for nucleic acid detection |
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| US6197510B1 (en) | 1998-10-01 | 2001-03-06 | Bio-Id Diagnostic Inc. | Multi-loci genomic analysis |
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| WO2003050304A1 (en) | 2001-12-08 | 2003-06-19 | Seegene, Inc | Annealing control primer system for regulating primer annealing specificity and its applications |
| US20030175709A1 (en) | 2001-12-20 | 2003-09-18 | Murphy George L. | Method and system for depleting rRNA populations |
| AU2003222178A1 (en) * | 2002-03-29 | 2003-10-13 | Nugen Technologies, Inc. | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
| US20030211483A1 (en) | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
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| CA2626977A1 (en) | 2005-10-27 | 2007-05-03 | Rosetta Inpharmatics Llc | Nucleic acid amplification using non-random primers |
| DE602006018352D1 (de) | 2005-12-06 | 2010-12-30 | Ambion Inc | Rückübertragungs-primer und verfahren zu deren entwurf |
| WO2009055732A1 (en) | 2007-10-26 | 2009-04-30 | Rosetta Inpharmatics Llc | Cdna synthesis using non-random primers |
-
2006
- 2006-12-06 DE DE602006018352T patent/DE602006018352D1/de active Active
- 2006-12-06 WO PCT/US2006/061643 patent/WO2007067907A1/en not_active Ceased
- 2006-12-06 EP EP10158848A patent/EP2206779B1/de not_active Not-in-force
- 2006-12-06 EP EP06846486A patent/EP1957645B1/de not_active Ceased
- 2006-12-06 AT AT06846486T patent/ATE488587T1/de not_active IP Right Cessation
-
2010
- 2010-11-19 US US12/950,179 patent/US8268987B2/en not_active Expired - Fee Related
-
2012
- 2012-08-22 US US13/592,278 patent/US8809513B2/en active Active
-
2014
- 2014-07-10 US US14/328,687 patent/US20150031584A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1957645B1 (de) | 2010-11-17 |
| EP2206779A1 (de) | 2010-07-14 |
| US8809513B2 (en) | 2014-08-19 |
| US20130059759A1 (en) | 2013-03-07 |
| US20110257039A1 (en) | 2011-10-20 |
| DE602006018352D1 (de) | 2010-12-30 |
| US20150031584A1 (en) | 2015-01-29 |
| EP1957645A1 (de) | 2008-08-20 |
| EP2206779B1 (de) | 2013-02-13 |
| WO2007067907A1 (en) | 2007-06-14 |
| US8268987B2 (en) | 2012-09-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |