ATE488770T1 - Cellulase von t. reesei mit verbesserter thermostabilität - Google Patents

Cellulase von t. reesei mit verbesserter thermostabilität

Info

Publication number
ATE488770T1
ATE488770T1 AT00984363T AT00984363T ATE488770T1 AT E488770 T1 ATE488770 T1 AT E488770T1 AT 00984363 T AT00984363 T AT 00984363T AT 00984363 T AT00984363 T AT 00984363T AT E488770 T1 ATE488770 T1 AT E488770T1
Authority
AT
Austria
Prior art keywords
cellulase
amino acid
reesei
improved
improved thermostability
Prior art date
Application number
AT00984363T
Other languages
English (en)
Inventor
Anthony Day
Colin Mitchinson
Andrew Shaw
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE488770T1 publication Critical patent/ATE488770T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT00984363T 1999-12-23 2000-12-14 Cellulase von t. reesei mit verbesserter thermostabilität ATE488770T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47083299A 1999-12-23 1999-12-23
PCT/US2000/033878 WO2001047956A2 (en) 1999-12-23 2000-12-14 Method for obtaining proteins having improved functional characteristics

Publications (1)

Publication Number Publication Date
ATE488770T1 true ATE488770T1 (de) 2010-12-15

Family

ID=23869235

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00984363T ATE488770T1 (de) 1999-12-23 2000-12-14 Cellulase von t. reesei mit verbesserter thermostabilität

Country Status (10)

Country Link
EP (2) EP2184350B1 (de)
JP (1) JP4658433B2 (de)
AT (1) ATE488770T1 (de)
AU (1) AU2099301A (de)
CA (1) CA2394740C (de)
DE (1) DE60045255D1 (de)
DK (2) DK2184350T3 (de)
ES (1) ES2354866T3 (de)
PT (1) PT1240524E (de)
WO (1) WO2001047956A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6407046B1 (en) 1998-09-03 2002-06-18 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6579841B1 (en) * 1998-12-18 2003-06-17 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
CN100491525C (zh) 2000-07-28 2009-05-27 汉高两合股份公司 从芽孢杆菌a7-7(dsm 12368)中提取的新型淀粉分解酶以及含有该新型淀粉分解酶的洗涤剂和清洗剂
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
US6623949B1 (en) 2000-08-04 2003-09-23 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6635465B1 (en) * 2000-08-04 2003-10-21 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
JP4907834B2 (ja) * 2000-08-04 2012-04-04 ダニスコ・ユーエス・インコーポレーテッド 変異体ｅｇｉｉｉセルラーゼ、そのようなｅｇｉｉｉ組成物をコードするｄｎａ及びそれを得るための方法
JP2003250544A (ja) 2002-03-04 2003-09-09 National Institute Of Technology & Evaluation タンパク質の性質を改変する方法
EP2385104A1 (de) * 2003-04-01 2011-11-09 Danisco US Inc. Abweichender Scytalidium thermophilium CBH1
JP2007532117A (ja) * 2004-04-08 2007-11-15 ジェネンコー・インターナショナル・インク αアミラーゼ変異体
US7629158B2 (en) * 2006-06-16 2009-12-08 The Procter & Gamble Company Cleaning and/or treatment compositions
EP2155870A2 (de) * 2007-05-30 2010-02-24 Danisco US, INC., Genencor Division Verbesserte varianten der alpha-amylase aus bacillus licheniformis
FI122028B (fi) * 2008-12-30 2011-07-29 Ab Enzymes Oy Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö
CA2771071C (en) * 2009-08-07 2020-03-10 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
WO2018150260A1 (en) * 2017-02-16 2018-08-23 Institut Pasteur Method for the identification of mutated proteins having modified thermal stability
EP4525615A2 (de) 2022-05-14 2025-03-26 Novozymes A/S Zusammensetzungen und verfahren zur prävention, behandlung, unterdrückung und/oder beseitigung von phytopathogenen befällen und infektionen

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
PT867504E (pt) * 1993-02-11 2003-08-29 Genencor Int Alfa-amilase estavel a oxidacao
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
DE69536145D1 (de) * 1994-03-08 2011-04-07 Novozymes As Neuartige alkalische Zellulasen
KR100511499B1 (ko) * 1995-02-03 2005-12-21 노보자임스 에이/에스 소정 특성을 가지는 알파-아밀라제 돌연변이체를 디자인하는 방법
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
US6211134B1 (en) * 1996-05-14 2001-04-03 Genecor International, Inc. Mutant α-amylase
CA2274806C (en) * 1996-12-09 2011-02-01 Genencor International, Inc. H mutant alpha-amylase enzymes
NZ505295A (en) * 1997-12-16 2002-12-20 Genencor Int A method of identifying cellulase gene sequences using conserved amino acid sequence fragments from known cellulase enzymes to provide primer nucleotide sequences
US6187732B1 (en) 1998-09-03 2001-02-13 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6623949B1 (en) * 2000-08-04 2003-09-23 Genencor International, Inc. Variant EGIII-like cellulase compositions

Also Published As

Publication number Publication date
JP4658433B2 (ja) 2011-03-23
JP2004500815A (ja) 2004-01-15
EP2184350A3 (de) 2010-06-02
CA2394740C (en) 2011-02-01
DE60045255D1 (de) 2010-12-30
WO2001047956A2 (en) 2001-07-05
EP2184350A2 (de) 2010-05-12
CA2394740A1 (en) 2001-07-05
PT1240524E (pt) 2011-02-02
EP1240524B1 (de) 2010-11-17
AU2099301A (en) 2001-07-09
DK2184350T3 (en) 2014-11-24
ES2354866T3 (es) 2011-03-18
EP2184350B1 (de) 2014-09-03
WO2001047956A3 (en) 2002-02-14
EP1240524A2 (de) 2002-09-18
DK1240524T3 (da) 2011-02-14

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