BRPI0509671A - alfa-amilases mutante - Google Patents

alfa-amilases mutante

Info

Publication number
BRPI0509671A
BRPI0509671A BRPI0509671-5A BRPI0509671A BRPI0509671A BR PI0509671 A BRPI0509671 A BR PI0509671A BR PI0509671 A BRPI0509671 A BR PI0509671A BR PI0509671 A BRPI0509671 A BR PI0509671A
Authority
BR
Brazil
Prior art keywords
amylases
mutant alpha
variant
amylase enzymes
mutant
Prior art date
Application number
BRPI0509671-5A
Other languages
English (en)
Inventor
Eugenio Ferrari
Marc Kolkman
Craig E Pilgrim
Original Assignee
Genencor Internation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Internation Inc filed Critical Genencor Internation Inc
Publication of BRPI0509671A publication Critical patent/BRPI0509671A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

ALFA-AMILASES MUTANTE. A presente invenção refere-se a novas enzimas de <244>-amilase variante nas quais os resíduos correspondentes a R179 r G180 em Bacillus stearothermophilus (SEQ ID NO: 2) são deletados. As enzimas de <244>-amilase variante descritas exibem estabilidade e/ou perfis de atividade alterados ou aumentados.
BRPI0509671-5A 2004-04-08 2005-04-08 alfa-amilases mutante BRPI0509671A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56112404P 2004-04-08 2004-04-08
PCT/US2005/012074 WO2005111203A2 (en) 2004-04-08 2005-04-08 MUTANT α ΑMYLASES

Publications (1)

Publication Number Publication Date
BRPI0509671A true BRPI0509671A (pt) 2007-10-30

Family

ID=35394736

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0509671-5A BRPI0509671A (pt) 2004-04-08 2005-04-08 alfa-amilases mutante

Country Status (7)

Country Link
US (3) US20060014265A1 (pt)
EP (1) EP1733032A2 (pt)
JP (1) JP2007532117A (pt)
CN (1) CN1938422A (pt)
BR (1) BRPI0509671A (pt)
CA (1) CA2562171A1 (pt)
WO (1) WO2005111203A2 (pt)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002094123A2 (en) 2001-05-18 2002-11-28 Danisco A/S Method of preparing a dought with an enzime
CN102533440B (zh) 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
US8431382B2 (en) 2006-05-31 2013-04-30 Novozymes A/S Chloramphenicol resistance selection in Bacillus licheniformis
US7968318B2 (en) 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
CN101489412B (zh) * 2006-07-13 2013-03-27 诺维信公司 细菌淀粉酶在牛类动物的饲料中的用途
WO2008088493A2 (en) 2006-12-21 2008-07-24 Danisco Us, Inc., Genencor Division Compositions and uses for an alpha-amylase polypeptide of bacillus species 195
CN102559627B (zh) * 2007-01-25 2014-09-10 杜邦营养生物科学有限公司 由地衣芽孢杆菌转化细胞制备脂酰基转移酶
CA2673954C (en) 2007-01-25 2015-09-15 Danisco A/S Production of a lipid acyltransferase from transformed bacillus licheniformis cells
ES2513217T3 (es) * 2007-02-07 2014-10-24 Danisco Us, Inc., Genencor Division Fitasas de Buttiauxella sp. variantes que tienen propiedades alteradas
US20080220498A1 (en) * 2007-03-06 2008-09-11 Cervin Marguerite A Variant Buttiauxella sp. phytases having altered properties
CN101680005A (zh) 2007-03-14 2010-03-24 丹尼斯科美国公司 从大麦产生乙醇和含有降低的β-葡聚糖和肌醇六磷酸的DDGS
MX2009009967A (es) * 2007-03-23 2009-10-07 Danisco Us Inc Genencor Div Produccion mejorada de amilasa por adicion n-terminal a proteina amilasa madura.
WO2009061380A2 (en) 2007-11-05 2009-05-14 Danisco Us Inc., Genencor Division VARIANTS OF BACILLUS sp. TS-23 ALPHA-AMYLASE WITH ALTERED PROPERTIES
AU2008325249B2 (en) * 2007-11-05 2013-10-10 Danisco Us Inc. Alpha-amylase variants with altered properties
US10431001B2 (en) * 2007-11-21 2019-10-01 Edda Technology, Inc. Method and system for interactive percutaneous pre-operation surgical planning
EP2235151B1 (en) 2007-12-21 2012-09-12 DuPont Nutrition Biosciences ApS Process for edible oil refining using a lipid acyltransferase
WO2009100138A2 (en) * 2008-02-04 2009-08-13 Danisco Us Inc., Genencor Division Variants of bacillus stearothermophilus alpha-amylase and uses thereof
MX2010008359A (es) 2008-02-04 2010-08-30 Danisco Us Inc Variantes de alfa-amilasa ts23 con propiedades alteradas.
MX2010011721A (es) 2008-04-30 2010-11-30 Danisco Inc Nuevas variantes de alfa-amilasa quimericas.
JP5599113B2 (ja) 2008-06-06 2014-10-01 ダニスコ・ユーエス・インク 糖化酵素組成物及びその糖化方法
MX2010013113A (es) * 2008-06-06 2010-12-21 Danisco Inc Variantes de alfa-amilasa geobacillus stearothermophilus con propiedades mejoradas.
BRPI0913378A2 (pt) * 2008-06-06 2015-09-01 Danisco Us Inc Produção de glicose a partir do amido usando alfa-amilase do bacillus subtilis
BRPI0915681A2 (pt) 2008-06-06 2015-08-04 Danisco Us Inc Composições e métodos compreendendo proteases microbianas variantes
MX350760B (es) * 2008-06-06 2017-09-18 Danisco Us Inc Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
CA2738447C (en) 2008-09-25 2019-01-08 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
US20120172275A1 (en) 2009-03-10 2012-07-05 Danisco Us Inc. Bacillus Megaterium Strain DSM90-Related Alpha-Amylases, and Methods of Use, Thereof
RU2011144134A (ru) * 2009-04-01 2013-05-10 ДАНИСКО ЮЭс ИНК. Композиции и способы, включающие варианты альфа-амилазы с измененными свойствами
CN102388131B (zh) 2009-04-08 2014-04-30 丹尼斯科美国公司 盐单胞菌属菌株WDG195相关α-淀粉酶及其使用方法
EP2430169A2 (en) * 2009-05-12 2012-03-21 Danisco US Inc. Ethanol yields in fermentation from an improved liquefaction process
CN102647918B (zh) 2009-08-07 2015-05-27 丹尼斯科美国公司 用于淀粉加工的α-淀粉酶混合物及其使用方法
CA2778471A1 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
CA2787962C (en) 2010-02-22 2020-06-02 Syngenta Participations Ag Methods for distinguishing and identifying plant varieties
CN103492579A (zh) 2011-04-29 2014-01-01 丹尼斯科美国公司 用纤维素酶和葡糖淀粉酶提高发酵制备乙醇的产率
JP2015528713A (ja) * 2012-06-21 2015-10-01 グローバス メディカル インコーポレイティッド 手術ロボットプラットフォーム
EP3321353A1 (en) 2012-12-11 2018-05-16 Danisco US Inc. Yeast host cells epxressing a glucoamylase from aspergillus fumigatus and methods of use thereof
WO2015057517A1 (en) 2013-10-17 2015-04-23 Danisco Us Inc. Use of hemicellulases to improve ethanol production
WO2015193479A1 (en) * 2014-06-19 2015-12-23 KB Medical SA Systems and methods for performing minimally invasive surgery
US20180030456A1 (en) * 2015-02-19 2018-02-01 Danisco Us Inc. Enhanced protein expression
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
KR20180069057A (ko) 2015-10-30 2018-06-22 다니스코 유에스 인크. 향상된 단백질 발현 및 이의 방법
EP3421596A4 (en) * 2016-02-26 2019-10-23 Nanjing Bestzyme Bio-engineering Co. Ltd. ALPHA AMYLASE VERSION AND USE THEREOF
DK3571217T5 (da) 2017-01-23 2024-09-02 Danisco Us Inc Fremgangsmåder og sammensætninger til opnåelse af naturlig kompetence i bacillus-værtsceller
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
CN108823186B (zh) * 2018-07-03 2022-11-22 江西省科学院微生物研究所 一种玉米淀粉降解能力提高的嗜热酸性生淀粉α-淀粉酶突变体及其制备方法和应用
CN110607305A (zh) * 2019-08-30 2019-12-24 海南大学 斑马鱼α7乙酰胆碱受体重组载体及重组细胞
WO2021257457A1 (en) 2020-06-15 2021-12-23 Bragg Analytics, Inc. Diffraction-based global in vitro diagnostic system
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
DE3688920D1 (de) * 1985-07-03 1993-09-30 Genencor Int Hybride Polypeptide und Verfahren zu deren Herstellung.
US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
AR000862A1 (es) * 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
AU2692897A (en) * 1996-04-30 1997-11-19 Novo Nordisk A/S Alpha-amylase mutants
US6361989B1 (en) * 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
WO1999019467A1 (en) * 1997-10-13 1999-04-22 Novo Nordisk A/S α-AMYLASE MUTANTS
US6309871B1 (en) * 1999-03-31 2001-10-30 Novozymes A/S Polypeptides having alkaline α-amylase activity
WO2001047956A2 (en) * 1999-12-23 2001-07-05 Genencor International, Inc. Method for obtaining proteins having improved functional characteristics
WO2001064852A1 (en) * 2000-03-03 2001-09-07 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
AU2001279254A1 (en) * 2000-08-11 2002-02-25 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries

Also Published As

Publication number Publication date
CA2562171A1 (en) 2005-11-24
EP1733032A2 (en) 2006-12-20
CN1938422A (zh) 2007-03-28
WO2005111203A2 (en) 2005-11-24
US20060014265A1 (en) 2006-01-19
JP2007532117A (ja) 2007-11-15
US20080160573A1 (en) 2008-07-03
WO2005111203A3 (en) 2006-03-30
US20100261232A1 (en) 2010-10-14

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 24, 8O C/C 13, 10 E 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

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