ATE489453T1 - Isolierung von bindungsproteinen mit hoher affinität für liganden - Google Patents

Isolierung von bindungsproteinen mit hoher affinität für liganden

Info

Publication number
ATE489453T1
ATE489453T1 AT01988762T AT01988762T ATE489453T1 AT E489453 T1 ATE489453 T1 AT E489453T1 AT 01988762 T AT01988762 T AT 01988762T AT 01988762 T AT01988762 T AT 01988762T AT E489453 T1 ATE489453 T1 AT E489453T1
Authority
AT
Austria
Prior art keywords
ligand
binding proteins
isolated
cells
binding
Prior art date
Application number
AT01988762T
Other languages
English (en)
Inventor
Gang Chen
Andrew Hayhurst
Jeffrey Thomas
Brent Iverson
George Georgiou
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Application granted granted Critical
Publication of ATE489453T1 publication Critical patent/ATE489453T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT01988762T 2000-10-27 2001-10-26 Isolierung von bindungsproteinen mit hoher affinität für liganden ATE489453T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/699,023 US7083945B1 (en) 2000-10-27 2000-10-27 Isolation of binding proteins with high affinity to ligands
PCT/US2001/046795 WO2002034886A2 (en) 2000-10-27 2001-10-26 Isolation of binding proteins with high affinity to ligands

Publications (1)

Publication Number Publication Date
ATE489453T1 true ATE489453T1 (de) 2010-12-15

Family

ID=24807610

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01988762T ATE489453T1 (de) 2000-10-27 2001-10-26 Isolierung von bindungsproteinen mit hoher affinität für liganden

Country Status (9)

Country Link
US (2) US7083945B1 (de)
EP (1) EP1356023B1 (de)
JP (2) JP4159872B2 (de)
AT (1) ATE489453T1 (de)
AU (2) AU3063502A (de)
CA (2) CA2645194C (de)
DE (1) DE60143545D1 (de)
DK (1) DK1356023T3 (de)
WO (1) WO2002034886A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7083945B1 (en) * 2000-10-27 2006-08-01 The Board Of Regents Of The University Of Texas System Isolation of binding proteins with high affinity to ligands
US7094571B2 (en) * 2000-10-27 2006-08-22 The Board Of Regents Of The University Of Texas System Combinatorial protein library screening by periplasmic expression
JP2006512891A (ja) * 2002-04-18 2006-04-20 ジェネンコー・インターナショナル・インク 糸状菌における機能性抗体の生産
US7601351B1 (en) 2002-06-26 2009-10-13 Human Genome Sciences, Inc. Antibodies against protective antigen
US7611866B2 (en) * 2002-07-15 2009-11-03 Board Of Regents, The University Of Texas System Selection of bacterial inner-membrane anchor polypeptides
AU2003304195B8 (en) * 2002-07-15 2008-08-28 Board Of Regents, The University Of Texas System Combinatorial protein library screening by periplasmic expression
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US20040248109A1 (en) * 2003-06-09 2004-12-09 Lawrence Greenfield Methods for selecting protein binding moieties
CA2560074A1 (en) * 2004-03-18 2005-11-03 Board Of Regents, The University Of Texas System Combinatorial protein library screening by periplasmic expression
US20060040327A1 (en) * 2004-08-18 2006-02-23 Terry Amiss Methods of screening proteins
DK2102239T3 (da) * 2006-11-30 2012-05-29 Res Dev Foundation Forbedrede immunoglobulin-biblioteker
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP3124497B1 (de) 2007-09-14 2020-04-15 Adimab, LLC Synthetische antikörperbibliotheken mit rationalem design und verwendungen davon
WO2010104115A1 (ja) * 2009-03-10 2010-09-16 独立行政法人産業技術総合研究所 膜タンパク質を特異的に認識するポリペプチドの調製方法
JP5972793B2 (ja) * 2009-12-23 2016-08-17 アフィニティ バイオサイエンス ピーティーワイ リミテッド タンパク質ディスプレイ
KR102318383B1 (ko) 2010-07-16 2021-10-27 아디맵 엘엘씨 항체 라이브러리
JP6153921B2 (ja) 2011-03-24 2017-06-28 オプコ ファーマシューティカルズ、エルエルシー ビーズまたは粒子ベースのライブラリーを用いた液体生体試料中でのバイオマーカー発見、並びに診断キットおよび治療法
AU2012276282B2 (en) * 2011-06-29 2015-11-05 Affinity Biosciences Pty Ltd Method of protein display
US20140243234A1 (en) * 2011-08-03 2014-08-28 Texas Biomedical Research Institute Nucleic acid compositions, methods and kits for rapid pairing of affinity agents
IN2014KN02831A (de) 2012-05-24 2015-05-08 Mountgate Group Ltd
CN106206166B (zh) * 2016-08-29 2018-07-10 厦门宏发信号电子有限公司 一种注塑轭铁的电磁继电器
EP3706795A4 (de) 2017-11-09 2021-10-13 Pinteon Therapeutics Inc. Verfahren und zusammensetzungen zur erzeugung und verwendung von humanisierten konformationsspezifischen phosphorylierten tau-antikörpern
IL275239B2 (en) 2017-12-11 2026-03-01 Abalone Bio Inc Yeast presentation of proteins in the subplasmic space
EP3938400B1 (de) 2019-03-11 2025-07-30 Memorial Sloan Kettering Cancer Center Cd22-antikörper und verfahren zur verwendung davon
JP7547478B2 (ja) * 2019-11-27 2024-09-09 イノバシオン・イ・デサロージョ・デ・エネルヒーア・アルファ・スステンタブレ・ソシエダ・アノニマ・デ・カピタル・バリアブレ ファージ耐性微生物
MX2023008285A (es) 2021-01-13 2023-09-12 Memorial Sloan Kettering Cancer Center Conjugado de anticuerpo anti-dll3-farmaco.
CA3204628A1 (en) 2021-01-13 2022-07-21 John T. POIRIER Antibody-pyrrolobenzodiazepine derivative conjugate

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
EP0177343B1 (de) 1984-10-05 1992-07-22 Genentech, Inc. DNA, Zellkulturen und Verfahren zur Sekretion von heterologen Proteinen und periplasmische Proteinrückgewinnung
US4946773A (en) 1985-12-23 1990-08-07 President And Fellows Of Harvard College Detection of base pair mismatches using RNAase A
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5160974A (en) 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US5780279A (en) 1990-12-03 1998-07-14 Genentech, Inc. Method of selection of proteolytic cleavage sites by directed evolution and phagemid display
DE69230142T2 (de) * 1991-05-15 2000-03-09 Cambridge Antibody Technology Ltd. Verfahren zur herstellung von spezifischen bindungspaargliedern
US7018809B1 (en) 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
AU687010B2 (en) * 1992-07-17 1998-02-19 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
US5521066A (en) 1993-09-13 1996-05-28 Bristol-Myers Squibb Company Periplasmic membrane-bound system for detecting protein-protein interactions
US5922545A (en) 1993-10-29 1999-07-13 Affymax Technologies N.V. In vitro peptide and antibody display libraries
AU704114B2 (en) * 1993-11-18 1999-04-15 Siga Technologies, Inc. Compounds and pharmaceutical compositions for the treatment and prophylaxis of bacterial infections
GB9408668D0 (en) 1994-04-30 1994-06-22 Orthosonics Ltd Untrasonic therapeutic system
NZ286241A (en) 1995-03-29 1997-11-24 Mitsui Chemicals Inc Method of producing a peptide as a fusion protein intracellularly which is cleaved on passing the cell membrane
US5702892A (en) 1995-05-09 1997-12-30 The United States Of America As Represented By The Department Of Health And Human Services Phage-display of immunoglobulin heavy chain libraries
US5955604A (en) * 1996-10-15 1999-09-21 The Regents Of The University Of California Substrates for β-lactamase and uses thereof
KR100570935B1 (ko) * 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
US6326204B1 (en) * 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AU7171098A (en) * 1997-05-01 1998-11-24 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US6342611B1 (en) * 1997-10-10 2002-01-29 Cytovia, Inc. Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for capsases and other enzymes and the use thereof
US6022952A (en) * 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
ATE276754T1 (de) * 1998-07-21 2004-10-15 Cytovia Inc Neue fluoreszensfarbstoffe und ihre anwendung in fluoreszensnachweisverfahren ganzer zellen für kaspasen, peptidasen, proteasen und andere enzyme sowie deren verwendung
US6180343B1 (en) * 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US6391575B1 (en) * 1999-03-05 2002-05-21 Idun Pharmaceuticals, Inc. Methods for detecting membrane derived caspase activity and modulators thereof
US7033781B1 (en) * 1999-09-29 2006-04-25 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
AU2000280285A1 (en) 2000-09-13 2002-03-26 California Institute Of Technology Oxygenase enzymes and screening method
US7083945B1 (en) * 2000-10-27 2006-08-01 The Board Of Regents Of The University Of Texas System Isolation of binding proteins with high affinity to ligands
US7094571B2 (en) * 2000-10-27 2006-08-22 The Board Of Regents Of The University Of Texas System Combinatorial protein library screening by periplasmic expression
AU2003304195B8 (en) 2002-07-15 2008-08-28 Board Of Regents, The University Of Texas System Combinatorial protein library screening by periplasmic expression
US7611866B2 (en) * 2002-07-15 2009-11-03 Board Of Regents, The University Of Texas System Selection of bacterial inner-membrane anchor polypeptides
WO2005095988A2 (en) 2004-03-18 2005-10-13 Board Of Regents, The University Of Texas System Selection of bacterial inner-membrane anchor polypeptides
CA2560074A1 (en) * 2004-03-18 2005-11-03 Board Of Regents, The University Of Texas System Combinatorial protein library screening by periplasmic expression

Also Published As

Publication number Publication date
US20070065913A1 (en) 2007-03-22
CA2645194A1 (en) 2002-05-02
DE60143545D1 (de) 2011-01-05
CA2645194C (en) 2012-02-07
CA2427305A1 (en) 2002-05-02
US7871796B2 (en) 2011-01-18
DK1356023T3 (da) 2011-03-14
EP1356023A2 (de) 2003-10-29
JP4159872B2 (ja) 2008-10-01
JP2008099710A (ja) 2008-05-01
US7083945B1 (en) 2006-08-01
WO2002034886A2 (en) 2002-05-02
JP2004531206A (ja) 2004-10-14
CA2427305C (en) 2011-10-04
EP1356023B1 (de) 2010-11-24
AU3063502A (en) 2002-05-06
AU2002230635B2 (en) 2007-02-08
WO2002034886A9 (en) 2003-02-13
WO2002034886A3 (en) 2003-07-31

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