ATE489473T1 - Analyse von boten-rna und mikro-rna im gleichen reaktionsansatz - Google Patents
Analyse von boten-rna und mikro-rna im gleichen reaktionsansatzInfo
- Publication number
- ATE489473T1 ATE489473T1 AT06787639T AT06787639T ATE489473T1 AT E489473 T1 ATE489473 T1 AT E489473T1 AT 06787639 T AT06787639 T AT 06787639T AT 06787639 T AT06787639 T AT 06787639T AT E489473 T1 ATE489473 T1 AT E489473T1
- Authority
- AT
- Austria
- Prior art keywords
- rna
- target
- hot start
- same reaction
- extension
- Prior art date
Links
- 238000006243 chemical reaction Methods 0.000 title abstract 3
- 108020004999 messenger RNA Proteins 0.000 title 1
- 108091070501 miRNA Proteins 0.000 title 1
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 238000010839 reverse transcription Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
- C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
- C12Q2549/101—Hot start
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US69993005P | 2005-07-15 | 2005-07-15 | |
| PCT/US2006/027757 WO2007011903A2 (en) | 2005-07-15 | 2006-07-17 | Analyzing messenger rna and micro rna in the same reaction mixture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE489473T1 true ATE489473T1 (de) | 2010-12-15 |
Family
ID=37669486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06787639T ATE489473T1 (de) | 2005-07-15 | 2006-07-17 | Analyse von boten-rna und mikro-rna im gleichen reaktionsansatz |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US7745122B2 (de) |
| EP (2) | EP1910549B1 (de) |
| AT (1) | ATE489473T1 (de) |
| DE (1) | DE602006018472D1 (de) |
| WO (1) | WO2007011903A2 (de) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE489473T1 (de) | 2005-07-15 | 2010-12-15 | Life Technologies Corp | Analyse von boten-rna und mikro-rna im gleichen reaktionsansatz |
| WO2007011901A2 (en) * | 2005-07-15 | 2007-01-25 | Applera Corporation | Hot start reverse transcription by primer design |
| US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US8039234B1 (en) * | 2006-05-22 | 2011-10-18 | Applied Biosystems, Llc | Methods, panels of identification markers, and kits for identifying forensic samples |
| EP2641976A3 (de) | 2009-01-16 | 2014-01-08 | Cepheid | Verfahren zur Erkennung von Gebärmutterhalskrebs |
| CN102388149A (zh) | 2009-02-02 | 2012-03-21 | 西菲伊德公司 | 检测败血症的方法 |
| WO2010099342A2 (en) | 2009-02-25 | 2010-09-02 | Cepheid | Methods of detecting lung cancer |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US8825412B2 (en) | 2010-05-18 | 2014-09-02 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| EP2656263B1 (de) | 2010-12-22 | 2019-11-06 | Natera, Inc. | Verfahren für nichtinvasiven pränatalen vaterschaftstest |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ， インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| WO2014093714A1 (en) | 2012-12-14 | 2014-06-19 | Bio-Rad Laboratories, Inc. | Methods and compositions for using oils for analysis and detection of molecules |
| WO2014164480A1 (en) | 2013-03-12 | 2014-10-09 | Cepheid | Methods of detecting cancer |
| EP2971103A1 (de) | 2013-03-14 | 2016-01-20 | Cepheid | Verfahren für den nachweis von lungenkrebs |
| CN113774132A (zh) | 2014-04-21 | 2021-12-10 | 纳特拉公司 | 检测染色体片段中的突变和倍性 |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| DK3294906T3 (en) | 2015-05-11 | 2024-08-05 | Natera Inc | Methods for determining ploidy |
| DK3443119T3 (da) | 2016-04-15 | 2022-05-23 | Natera Inc | Fremgangsmåde til bestemmelse af lunge cancer |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| WO2019118926A1 (en) | 2017-12-14 | 2019-06-20 | Tai Diagnostics, Inc. | Assessing graft suitability for transplantation |
| EP4671380A3 (de) * | 2018-01-12 | 2026-02-25 | Natera, Inc. | Neue primer und verwendungen davon |
| DK3781714T3 (da) | 2018-04-14 | 2026-03-30 | Natera Inc | Fremgangsmåder til cancerpåvisning og -overvågning ved personlig påvisning af cirkulerende tumor-dna |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| CN113396326A (zh) | 2018-12-19 | 2021-09-14 | 核酸有限责任公司 | 用于分子诊断的设备和方法 |
| EP3980559A1 (de) | 2019-06-06 | 2022-04-13 | Natera, Inc. | Verfahren zur detektion von immunzellen-dna und zur überwachung des immunsystems |
| EP4118230A4 (de) | 2020-03-09 | 2024-04-24 | Nuclein, LLC | Vorrichtung und verfahren zur molekularen diagnose |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| WO1991012342A1 (en) | 1990-02-16 | 1991-08-22 | F. Hoffmann-La Roche Ag | Improvements in the specificity and convenience of the polymerase chain reaction |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US6043060A (en) | 1996-11-18 | 2000-03-28 | Imanishi; Takeshi | Nucleotide analogues |
| JP3756313B2 (ja) | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
| CA2303299C (en) | 1997-09-12 | 2016-02-23 | Exiqon A/S | Oligonucleotide analogues |
| TNSN99233A1 (fr) | 1998-12-19 | 2001-12-31 | Exxon Production Research Co | Aciers de haute resistance avec excellente tenacite de temperature cryogenique |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| EP3000899A1 (de) | 2002-12-04 | 2016-03-30 | Applied Biosystems, LLC | Multiplexverstärkung von polynucleotiden |
| US7604937B2 (en) * | 2004-03-24 | 2009-10-20 | Applied Biosystems, Llc | Encoding and decoding reactions for determining target polynucleotides |
| US8192937B2 (en) * | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
| US7575863B2 (en) * | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| ES2435771T3 (es) * | 2004-07-19 | 2013-12-23 | Smith & Nephew, Inc. | Sinterización por corriente pulsada para superficies de implantes médicos |
| US20060057595A1 (en) * | 2004-09-16 | 2006-03-16 | Applera Corporation | Compositions, methods, and kits for identifying and quantitating small RNA molecules |
| US20070015176A1 (en) * | 2005-02-18 | 2007-01-18 | Applera Corporation | Small nucleic acid detection probes and uses thereof |
| US20060223066A1 (en) * | 2005-03-29 | 2006-10-05 | Applera Corporation | Methods for normalizing and for identifying small nucleic acids |
| WO2007117256A1 (en) * | 2005-05-31 | 2007-10-18 | Applera Corporation | Multiplexed amplification of short nucleic acids |
| US20070048757A1 (en) * | 2005-05-31 | 2007-03-01 | Applera Corporation | Methods for characterizing cells using amplified micro rnas |
| US7556943B2 (en) * | 2005-05-31 | 2009-07-07 | Applied Biosystems, Llc | Linear amplification of short nucleic acids |
| WO2007011902A2 (en) * | 2005-07-15 | 2007-01-25 | Applera Corporation | Methods for rna profiling |
| WO2007011901A2 (en) * | 2005-07-15 | 2007-01-25 | Applera Corporation | Hot start reverse transcription by primer design |
| ATE489473T1 (de) * | 2005-07-15 | 2010-12-15 | Life Technologies Corp | Analyse von boten-rna und mikro-rna im gleichen reaktionsansatz |
| WO2007032817A2 (en) * | 2005-09-12 | 2007-03-22 | Applera Corporation | Pure mirna sample preparation method |
| FR2989691B1 (fr) | 2012-04-24 | 2014-05-23 | Commissariat Energie Atomique | Reacteur pour le depot de couche atomique (ald), application a l'encapsulage d'un dispositif oled par depot de couche transparente en al2o3. |
| JP6102810B2 (ja) | 2014-03-25 | 2017-03-29 | 富士ゼロックス株式会社 | 帯電部材、帯電装置、プロセスカートリッジ、画像形成装置、および帯電部材の製造方法 |
-
2006
- 2006-07-17 AT AT06787639T patent/ATE489473T1/de not_active IP Right Cessation
- 2006-07-17 EP EP06787639A patent/EP1910549B1/de not_active Not-in-force
- 2006-07-17 WO PCT/US2006/027757 patent/WO2007011903A2/en not_active Ceased
- 2006-07-17 DE DE602006018472T patent/DE602006018472D1/de active Active
- 2006-07-17 US US11/458,089 patent/US7745122B2/en active Active
- 2006-07-17 EP EP10191745A patent/EP2308990B1/de not_active Not-in-force
-
2010
- 2010-05-17 US US12/781,690 patent/US8487085B2/en active Active
-
2013
- 2013-07-15 US US13/942,437 patent/US8962254B2/en not_active Expired - Fee Related
-
2015
- 2015-02-20 US US14/627,741 patent/US9422603B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20140065624A1 (en) | 2014-03-06 |
| EP1910549B1 (de) | 2010-11-24 |
| EP2308990B1 (de) | 2012-09-26 |
| EP1910549A2 (de) | 2008-04-16 |
| EP2308990A1 (de) | 2011-04-13 |
| US20100221790A1 (en) | 2010-09-02 |
| US8962254B2 (en) | 2015-02-24 |
| DE602006018472D1 (de) | 2011-01-05 |
| US8487085B2 (en) | 2013-07-16 |
| EP1910549A4 (de) | 2009-03-25 |
| WO2007011903A3 (en) | 2007-07-12 |
| US7745122B2 (en) | 2010-06-29 |
| US20150232918A1 (en) | 2015-08-20 |
| US20070128621A1 (en) | 2007-06-07 |
| WO2007011903A2 (en) | 2007-01-25 |
| US9422603B2 (en) | 2016-08-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |