ATE318931T1 - ßSHOT GUNß SEQUENZIERUNG UND AMPLIFIZIERUNG OHNE KLONIERUNG - Google Patents
ßSHOT GUNß SEQUENZIERUNG UND AMPLIFIZIERUNG OHNE KLONIERUNGInfo
- Publication number
- ATE318931T1 ATE318931T1 AT00928263T AT00928263T ATE318931T1 AT E318931 T1 ATE318931 T1 AT E318931T1 AT 00928263 T AT00928263 T AT 00928263T AT 00928263 T AT00928263 T AT 00928263T AT E318931 T1 ATE318931 T1 AT E318931T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- sequence
- acid template
- primer
- template
- Prior art date
Links
- 238000012163 sequencing technique Methods 0.000 title abstract 2
- 230000003321 amplification Effects 0.000 title 1
- 238000010367 cloning Methods 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 8
- 102000039446 nucleic acids Human genes 0.000 abstract 8
- 108020004707 nucleic acids Proteins 0.000 abstract 8
- 238000000034 method Methods 0.000 abstract 3
- 230000000295 complement effect Effects 0.000 abstract 2
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 238000000137 annealing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Treating Waste Gases (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13035899P | 1999-04-21 | 1999-04-21 | |
| US09/434,761 US6521428B1 (en) | 1999-04-21 | 1999-11-04 | Shot-gun sequencing and amplification without cloning |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE318931T1 true ATE318931T1 (de) | 2006-03-15 |
Family
ID=26828421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00928263T ATE318931T1 (de) | 1999-04-21 | 2000-04-20 | ßSHOT GUNß SEQUENZIERUNG UND AMPLIFIZIERUNG OHNE KLONIERUNG |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US6521428B1 (de) |
| EP (1) | EP1183388B1 (de) |
| AT (1) | ATE318931T1 (de) |
| AU (1) | AU4652300A (de) |
| DE (1) | DE60026294T2 (de) |
| WO (1) | WO2000063439A2 (de) |
Families Citing this family (79)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6780591B2 (en) * | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6521428B1 (en) * | 1999-04-21 | 2003-02-18 | Genome Technologies, Llc | Shot-gun sequencing and amplification without cloning |
| AUPQ008799A0 (en) * | 1999-04-30 | 1999-05-27 | Tillett, Daniel | Genome sequencing |
| US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US7501245B2 (en) * | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| US6846626B1 (en) * | 1999-09-01 | 2005-01-25 | Genome Technologies, Llc | Method for amplifying sequences from unknown DNA |
| US6221600B1 (en) * | 1999-10-08 | 2001-04-24 | Board Of Regents, The University Of Texas System | Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis |
| EP1276901A2 (de) * | 2000-01-13 | 2003-01-22 | Amsterdam Support Diagnostics B.V. | Ein universelles nukleinsäure-amplifikationssystem für nukleinsäuren in einer probe |
| JP2004523243A (ja) | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
| US6949340B2 (en) * | 2001-03-28 | 2005-09-27 | Creative Mines Llc | Optical phase modulator |
| US7138506B2 (en) | 2001-05-09 | 2006-11-21 | Genetic Id, Na, Inc. | Universal microarray system |
| WO2003040395A2 (en) | 2001-11-07 | 2003-05-15 | Applera Corporation | Universal nucleotides for nucleic acid analysis |
| US7115370B2 (en) | 2002-06-05 | 2006-10-03 | Capital Genomix, Inc. | Combinatorial oligonucleotide PCR |
| US9388459B2 (en) * | 2002-06-17 | 2016-07-12 | Affymetrix, Inc. | Methods for genotyping |
| US7459273B2 (en) * | 2002-10-04 | 2008-12-02 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
| EP1418241A1 (de) * | 2002-11-08 | 2004-05-12 | PrimaGen Holding B.V. | Methode zur Quantifizierung des Verhältnisses zweier Nukleinsäuresequenzen |
| US8206913B1 (en) | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| US7718403B2 (en) * | 2003-03-07 | 2010-05-18 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| US20050170367A1 (en) * | 2003-06-10 | 2005-08-04 | Quake Stephen R. | Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids |
| US8114978B2 (en) | 2003-08-05 | 2012-02-14 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
| US20050097548A1 (en) * | 2003-10-31 | 2005-05-05 | Dillenburg Brian J. | Systems and methods for developing and distributing software components |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| US20060172408A1 (en) * | 2003-12-01 | 2006-08-03 | Quake Steven R | Device for immobilizing chemical and biochemical species and methods of using same |
| ATE463584T1 (de) | 2004-02-19 | 2010-04-15 | Helicos Biosciences Corp | Verfahren zur analyse von polynukleotidsequenzen |
| US20060046258A1 (en) * | 2004-02-27 | 2006-03-02 | Lapidus Stanley N | Applications of single molecule sequencing |
| EP2380993B1 (de) | 2004-03-08 | 2015-12-23 | Rubicon Genomics, Inc. | Verfahren zur Erstellung und Erweiterung von DNA-Bibliotheken zur sensitiven Erkennung und Analyse von DNA-Methylierung |
| US20050239085A1 (en) * | 2004-04-23 | 2005-10-27 | Buzby Philip R | Methods for nucleic acid sequence determination |
| US20050260609A1 (en) * | 2004-05-24 | 2005-11-24 | Lapidus Stanley N | Methods and devices for sequencing nucleic acids |
| US7476734B2 (en) * | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US20070117103A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| US20070117104A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| EP1766090B1 (de) * | 2004-05-25 | 2011-04-27 | Helicos Biosciences Corporation | Verfahren zur nukleinsäureimmobilisierung |
| US20060024678A1 (en) * | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
| WO2006073504A2 (en) * | 2004-08-04 | 2006-07-13 | President And Fellows Of Harvard College | Wobble sequencing |
| US20060118754A1 (en) * | 2004-12-08 | 2006-06-08 | Lapen Daniel C | Stabilizing a polyelectrolyte multilayer |
| US7220549B2 (en) * | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US20060172328A1 (en) * | 2005-01-05 | 2006-08-03 | Buzby Philip R | Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction |
| US7482120B2 (en) * | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US8084207B2 (en) * | 2005-03-03 | 2011-12-27 | Ibis Bioscience, Inc. | Compositions for use in identification of papillomavirus |
| US20060263790A1 (en) * | 2005-05-20 | 2006-11-23 | Timothy Harris | Methods for improving fidelity in a nucleic acid synthesis reaction |
| DK1907571T3 (en) | 2005-06-15 | 2017-08-21 | Complete Genomics Inc | NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS |
| EP1924705A1 (de) | 2005-08-02 | 2008-05-28 | Rubicon Genomics, Inc. | Isolierung von cpg-inseln durch thermische segregation und enzymatisches selektions-amplifikations-verfahren |
| DK1924704T3 (da) | 2005-08-02 | 2011-09-05 | Rubicon Genomics Inc | Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion |
| EP1917346A2 (de) * | 2005-08-05 | 2008-05-07 | Helicos Biosciences Corporation | Signalmusterzusammensetzungen und -verfahren |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US20070065834A1 (en) * | 2005-09-19 | 2007-03-22 | Hillis William D | Method and sequences for determinate nucleic acid hybridization |
| US20070117102A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| US20070128610A1 (en) * | 2005-12-02 | 2007-06-07 | Buzby Philip R | Sample preparation method and apparatus for nucleic acid sequencing |
| US11306351B2 (en) | 2005-12-21 | 2022-04-19 | Affymetrix, Inc. | Methods for genotyping |
| EP1987159B2 (de) | 2006-02-08 | 2020-08-12 | Illumina Cambridge Limited | Verfahren zur sequenzierung einer polynukleotidmatrize |
| US7397546B2 (en) * | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US20080309926A1 (en) * | 2006-03-08 | 2008-12-18 | Aaron Weber | Systems and methods for reducing detected intensity non uniformity in a laser beam |
| US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| US20080293589A1 (en) * | 2007-05-24 | 2008-11-27 | Affymetrix, Inc. | Multiplex locus specific amplification |
| US20100248310A1 (en) * | 2007-10-22 | 2010-09-30 | Monoquant Pty Ltd. | Method of dna amplification |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| US20090247426A1 (en) * | 2008-03-31 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Focused library generation |
| WO2010027870A2 (en) * | 2008-08-26 | 2010-03-11 | Fluidigm Corporation | Assay methods for increased throughput of samples and/or targets |
| US8795961B2 (en) * | 2008-09-05 | 2014-08-05 | Pacific Biosciences Of California, Inc. | Preparations, compositions, and methods for nucleic acid sequencing |
| CN103952482A (zh) | 2009-04-02 | 2014-07-30 | 弗卢伊蒂格姆公司 | 用于对目标核酸进行条形码化的多引物扩增方法 |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| DK2456892T3 (en) * | 2009-07-24 | 2014-12-08 | Illumina Inc | Procedure for sequencing of a polynukleotidskabelon |
| US9194838B2 (en) | 2010-03-03 | 2015-11-24 | Osaka University | Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide |
| SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| CN103890191B (zh) * | 2011-05-27 | 2018-12-04 | 哈佛大学校长及研究员协会 | 单细胞全基因组扩增方法 |
| WO2013147208A1 (ja) * | 2012-03-29 | 2013-10-03 | 国立大学法人大阪大学 | ポリヌクレオチドの塩基配列を決定する方法、および、ポリヌクレオチドの塩基配列を決定する装置 |
| CN104471077B (zh) | 2012-05-21 | 2017-05-24 | 富鲁达公司 | 颗粒群的单颗粒分析 |
| US9535033B2 (en) | 2012-08-17 | 2017-01-03 | Quantum Biosystems Inc. | Sample analysis method |
| JP6282036B2 (ja) | 2012-12-27 | 2018-02-21 | クオンタムバイオシステムズ株式会社 | 物質の移動速度の制御方法および制御装置 |
| EP3047282B1 (de) | 2013-09-18 | 2019-05-15 | Quantum Biosystems Inc. | Biomolekülsequenzierungsvorrichtungen, -systeme und -verfahren |
| US10100349B2 (en) * | 2013-09-30 | 2018-10-16 | President And Fellows Of Harvard College | Methods of determining polymorphisms |
| JP2015077652A (ja) | 2013-10-16 | 2015-04-23 | クオンタムバイオシステムズ株式会社 | ナノギャップ電極およびその製造方法 |
| US10438811B1 (en) | 2014-04-15 | 2019-10-08 | Quantum Biosystems Inc. | Methods for forming nano-gap electrodes for use in nanosensors |
| WO2016201142A1 (en) | 2015-06-09 | 2016-12-15 | Life Technologies Corporation | Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging |
| CA3006994A1 (en) | 2015-12-16 | 2017-06-22 | Fluidigm Corporation | High-level multiplex amplification |
| CN105925675B (zh) * | 2016-04-26 | 2020-06-05 | 序康医疗科技(苏州)有限公司 | 扩增dna的方法 |
| CN115595360A (zh) | 2016-04-27 | 2023-01-13 | 因美纳剑桥有限公司(Gb) | 用于生物分子的测量和测序的系统和方法 |
| WO2018027004A1 (en) | 2016-08-03 | 2018-02-08 | Pierce Biotechnology, Inc. | Chemiluminescent and fluorescent substrate pads and uses thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5104792A (en) * | 1989-12-21 | 1992-04-14 | The United States Of America As Represented By The Department Of Health And Human Services | Method for amplifying unknown nucleic acid sequences |
| US5506100A (en) * | 1990-10-11 | 1996-04-09 | Indiana University Foundation | Process and apparatus for fragmenting biomaterials |
| JPH0622798A (ja) * | 1992-07-07 | 1994-02-01 | Hitachi Ltd | 塩基配列決定法 |
| DE59502334D1 (de) * | 1994-01-07 | 1998-07-02 | Qiagen Gmbh | Verfahren zum zerkleinern von hochmolekularen strukturen |
| US5552278A (en) * | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
| WO1997001645A1 (en) * | 1995-06-28 | 1997-01-16 | Amersham Life Science | Primer walking cycle sequencing |
| US5807679A (en) * | 1995-07-07 | 1998-09-15 | Myriad Genetics, Inc. | Island hopping--a method to sequence rapidly very large fragments of DNA |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| CA2234736A1 (en) * | 1996-07-16 | 1998-01-22 | Periannan Senapathy | Method for contiguous genome sequencing |
| US6521428B1 (en) * | 1999-04-21 | 2003-02-18 | Genome Technologies, Llc | Shot-gun sequencing and amplification without cloning |
-
1999
- 1999-11-04 US US09/434,761 patent/US6521428B1/en not_active Expired - Lifetime
-
2000
- 2000-04-20 AT AT00928263T patent/ATE318931T1/de not_active IP Right Cessation
- 2000-04-20 EP EP00928263A patent/EP1183388B1/de not_active Expired - Lifetime
- 2000-04-20 WO PCT/US2000/010745 patent/WO2000063439A2/en not_active Ceased
- 2000-04-20 DE DE60026294T patent/DE60026294T2/de not_active Expired - Lifetime
- 2000-04-20 AU AU46523/00A patent/AU4652300A/en not_active Abandoned
-
2001
- 2001-05-07 US US09/850,684 patent/US6528288B2/en not_active Expired - Lifetime
-
2002
- 2002-12-04 US US10/309,516 patent/US7056706B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US6528288B2 (en) | 2003-03-04 |
| DE60026294T2 (de) | 2006-12-28 |
| WO2000063439A3 (en) | 2001-03-29 |
| US20030100006A1 (en) | 2003-05-29 |
| EP1183388B1 (de) | 2006-03-01 |
| US6521428B1 (en) | 2003-02-18 |
| US20010046681A1 (en) | 2001-11-29 |
| AU4652300A (en) | 2000-11-02 |
| WO2000063439A2 (en) | 2000-10-26 |
| US7056706B2 (en) | 2006-06-06 |
| DE60026294D1 (de) | 2006-04-27 |
| EP1183388A2 (de) | 2002-03-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |