ATE489474T1 - Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften - Google Patents

Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften

Info

Publication number
ATE489474T1
ATE489474T1 AT03765839T AT03765839T ATE489474T1 AT E489474 T1 ATE489474 T1 AT E489474T1 AT 03765839 T AT03765839 T AT 03765839T AT 03765839 T AT03765839 T AT 03765839T AT E489474 T1 ATE489474 T1 AT E489474T1
Authority
AT
Austria
Prior art keywords
genes
protein production
production
useful
generating
Prior art date
Application number
AT03765839T
Other languages
English (en)
Inventor
Luigi Grasso
Bradford J Kline
Nicholas Nicolaides
Philip Sass
Original Assignee
Morphotek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphotek Inc filed Critical Morphotek Inc
Application granted granted Critical
Publication of ATE489474T1 publication Critical patent/ATE489474T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT03765839T 2002-07-19 2003-07-21 Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften ATE489474T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39702702P 2002-07-19 2002-07-19
PCT/US2003/022743 WO2004009782A2 (en) 2002-07-19 2003-07-21 Methods for generating enhanced antibody-producing cell lines with improved growth characteristics

Publications (1)

Publication Number Publication Date
ATE489474T1 true ATE489474T1 (de) 2010-12-15

Family

ID=30770977

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03765839T ATE489474T1 (de) 2002-07-19 2003-07-21 Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften

Country Status (8)

Country Link
US (2) US20040115695A1 (de)
EP (1) EP1551988B1 (de)
JP (1) JP4527531B2 (de)
AT (1) ATE489474T1 (de)
AU (1) AU2003256642B2 (de)
CA (1) CA2493679A1 (de)
DE (1) DE60335129D1 (de)
WO (1) WO2004009782A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004046330A2 (en) * 2002-11-15 2004-06-03 Morphotek, Inc. Methods of generating high-production of antibodies from hybridomas created by in vitro immunization
CA2556027C (en) * 2004-02-12 2015-11-24 Morphotek, Inc. Monoclonal antibodies that specifically block biological activity of a tumor antigen
JP2008505943A (ja) 2004-07-14 2008-02-28 イゲネーオン・クレープス−イムンテラピー・フォルシュングス−ウント・エントヴィックルングス−アクチェンゲゼルシャフト グリコシル化抗体
EP2322560B1 (de) 2005-03-10 2013-09-04 Morphotek, Inc. Anti-Mesothelin-Antikörper
EP2172487A1 (de) 2005-04-22 2010-04-07 Morphotek Inc. Antikörper mit Immuneffektoraktivität, die in Folatrezeptor alpha-positive Zellen internalisiert werden
EP2013620A2 (de) * 2006-04-17 2009-01-14 Morphotek, Inc. Ganzgenomevolutionstechnologie für verbesserte protein- und antikörperausbeute durch zellen
WO2008089355A1 (en) * 2007-01-17 2008-07-24 Life Technologies Corporation Methods and compositions for improving the health of cells in culture
JP5675109B2 (ja) * 2007-02-20 2015-02-25 アナプティスバイオ インコーポレイティッド ライブラリーの生成方法及びその使用
CN104321337B (zh) * 2012-05-25 2017-07-11 永林有限公司 肽及其用途

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4732973A (en) * 1984-06-14 1988-03-22 Chiron Corporation Active site modified protease α-1-antitrypsin inhibitors
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein
US5530101A (en) * 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6191268B1 (en) * 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
US5907079A (en) * 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US5885827A (en) * 1996-01-23 1999-03-23 The Regents Of The Universtiy Of California Eukaryotic high rate mutagenesis system
US6326204B1 (en) * 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP1717322B1 (de) * 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution ganzer Zellen durch rekursive Sequenzrekombination
US20020012991A1 (en) * 1997-04-07 2002-01-31 Florence Chua Nee Ho Kit Fong Cell culture media for enhanced protein production
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
US6890730B1 (en) * 1999-12-10 2005-05-10 The Salk Institute Sequence and method for increasing protein expression in cellular expression systems
US6825038B2 (en) * 2000-05-12 2004-11-30 The Johns Hopkins University Method for generating hypermutable organisms
DE60045652D1 (de) * 2000-11-07 2011-03-31 Morphotek Inc Verfahren zur Erzeugung von genetisch veränderten Antikörperherstellungszelllinien mit verbesserter Antikörpereigenschaft
JP5036119B2 (ja) * 2001-01-15 2012-09-26 モーフオテク・インコーポレーテツド ミスマッチ修復の化学阻害剤

Also Published As

Publication number Publication date
DE60335129D1 (de) 2011-01-05
JP4527531B2 (ja) 2010-08-18
US20090155797A1 (en) 2009-06-18
WO2004009782A3 (en) 2004-09-23
AU2003256642A1 (en) 2004-02-09
JP2005533499A (ja) 2005-11-10
EP1551988B1 (de) 2010-11-24
AU2003256642A2 (en) 2004-02-09
EP1551988A4 (de) 2007-11-14
WO2004009782A2 (en) 2004-01-29
AU2003256642B2 (en) 2008-05-01
EP1551988A2 (de) 2005-07-13
US20040115695A1 (en) 2004-06-17
CA2493679A1 (en) 2004-01-29

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