ATE489474T1 - Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften - Google Patents

Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften

Info

Publication number
ATE489474T1
ATE489474T1 AT03765839T AT03765839T ATE489474T1 AT E489474 T1 ATE489474 T1 AT E489474T1 AT 03765839 T AT03765839 T AT 03765839T AT 03765839 T AT03765839 T AT 03765839T AT E489474 T1 ATE489474 T1 AT E489474T1
Authority
AT
Austria
Prior art keywords
genes
protein production
production
useful
generating
Prior art date
Application number
AT03765839T
Other languages
English (en)
Inventor
Luigi Grasso
Bradford J Kline
Nicholas Nicolaides
Philip Sass
Original Assignee
Morphotek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphotek Inc filed Critical Morphotek Inc
Application granted granted Critical
Publication of ATE489474T1 publication Critical patent/ATE489474T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT03765839T 2002-07-19 2003-07-21 Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften ATE489474T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39702702P 2002-07-19 2002-07-19
PCT/US2003/022743 WO2004009782A2 (en) 2002-07-19 2003-07-21 Methods for generating enhanced antibody-producing cell lines with improved growth characteristics

Publications (1)

Publication Number Publication Date
ATE489474T1 true ATE489474T1 (de) 2010-12-15

Family

ID=30770977

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03765839T ATE489474T1 (de) 2002-07-19 2003-07-21 Verfahren zur erzeugung verbesserter antikörper produzierender zellinien mit verbesserten wachstumseigenschaften

Country Status (8)

Country Link
US (2) US20040115695A1 (de)
EP (1) EP1551988B1 (de)
JP (1) JP4527531B2 (de)
AT (1) ATE489474T1 (de)
AU (1) AU2003256642B2 (de)
CA (1) CA2493679A1 (de)
DE (1) DE60335129D1 (de)
WO (1) WO2004009782A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4555089B2 (ja) 2002-11-15 2010-09-29 モーフオテク・インコーポレーテツド ｉｎｖｉｔｒｏ免疫感作により創製されたハイブリドーマからの抗体の高生産量の生成方法
EP3101034A1 (de) * 2004-02-12 2016-12-07 Morphotek, Inc. Monoklonale, spezifisch an folatrezeptor-alpha bindende antikörper
US7759464B2 (en) 2004-07-14 2010-07-20 Greenovation Biotech Gmbh N-glycosylated antibody
EP1861425B1 (de) 2005-03-10 2012-05-16 Morphotek, Inc. Anti-mesothelin-antikörper
CA2607444C (en) 2005-04-22 2015-03-10 Morphotek, Inc. Antibodies with immune effector activity and that internalize in folate receptor alpha-positive cells
JP2009533072A (ja) * 2006-04-17 2009-09-17 モルフォテック、インク． 細胞によるタンパク質および抗体収量を改善するために適用される全ゲノム進化技術
EP2115127A4 (de) * 2007-01-17 2010-04-07 Life Technologies Corp Verfahren und zusammensetzungen zur verbesserung der zellgesundheit in kulturen
US20090075378A1 (en) * 2007-02-20 2009-03-19 Anaptysbio, Inc. Somatic hypermutation systems
HUE033389T2 (hu) * 2012-05-25 2017-11-28 Well Resources Ltd Peptid és annak alkalmazása

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4732973A (en) * 1984-06-14 1988-03-22 Chiron Corporation Active site modified protease α-1-antitrypsin inhibitors
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein
US5530101A (en) * 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6191268B1 (en) * 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
US5907079A (en) * 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US5885827A (en) * 1996-01-23 1999-03-23 The Regents Of The Universtiy Of California Eukaryotic high rate mutagenesis system
KR100570935B1 (ko) * 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
US6326204B1 (en) * 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US20020012991A1 (en) * 1997-04-07 2002-01-31 Florence Chua Nee Ho Kit Fong Cell culture media for enhanced protein production
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
US6890730B1 (en) * 1999-12-10 2005-05-10 The Salk Institute Sequence and method for increasing protein expression in cellular expression systems
WO2001088192A2 (en) * 2000-05-12 2001-11-22 The Johns Hopkins University A method for generating hypermutable organisms
EP1345495B1 (de) * 2000-11-07 2009-02-25 Morphotek Inc. Verfahren zur herstellung von genetisch modifizierten antikörperproduzierenden zelllinien mit verbesserten antikörpereigenschaften
WO2002054856A1 (en) * 2001-01-15 2002-07-18 Morphotek Inc. Chemical inhibitors of mismatch repair

Also Published As

Publication number Publication date
AU2003256642A2 (en) 2004-02-09
WO2004009782A2 (en) 2004-01-29
EP1551988A4 (de) 2007-11-14
JP4527531B2 (ja) 2010-08-18
EP1551988A2 (de) 2005-07-13
JP2005533499A (ja) 2005-11-10
DE60335129D1 (de) 2011-01-05
AU2003256642B2 (en) 2008-05-01
US20040115695A1 (en) 2004-06-17
AU2003256642A1 (en) 2004-02-09
CA2493679A1 (en) 2004-01-29
EP1551988B1 (de) 2010-11-24
WO2004009782A3 (en) 2004-09-23
US20090155797A1 (en) 2009-06-18

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Legal Events

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