ATE490309T1 - Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon - Google Patents

Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon

Info

Publication number
ATE490309T1
ATE490309T1 AT03785159T AT03785159T ATE490309T1 AT E490309 T1 ATE490309 T1 AT E490309T1 AT 03785159 T AT03785159 T AT 03785159T AT 03785159 T AT03785159 T AT 03785159T AT E490309 T1 ATE490309 T1 AT E490309T1
Authority
AT
Austria
Prior art keywords
zymes
phytase
methods
mutant
coli
Prior art date
Application number
AT03785159T
Other languages
English (en)
Inventor
Beat Blattmann
Aldis Darzins
John Davis
Lance Encell
Thomas Morrison
Gregory Mrachko
Volker Schellenberger
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of ATE490309T1 publication Critical patent/ATE490309T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT03785159T 2002-08-12 2003-08-11 Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon ATE490309T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40333002P 2002-08-12 2002-08-12
PCT/US2003/025058 WO2004015084A2 (en) 2002-08-12 2003-08-11 Mutant e. coli appa phytase enzymes

Publications (1)

Publication Number Publication Date
ATE490309T1 true ATE490309T1 (de) 2010-12-15

Family

ID=31715975

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03785159T ATE490309T1 (de) 2002-08-12 2003-08-11 Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon

Country Status (9)

Country Link
US (1) US7968342B2 (de)
EP (1) EP1546316B1 (de)
CN (2) CN101792749B (de)
AT (1) ATE490309T1 (de)
AU (1) AU2003258157A1 (de)
CA (1) CA2495660A1 (de)
DE (1) DE60335195D1 (de)
DK (1) DK1546316T3 (de)
WO (1) WO2004015084A2 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8114443B2 (en) * 2002-03-13 2012-02-14 Academia Sinica Phytase-expressing transgenic plants
DE60335195D1 (de) * 2002-08-12 2011-01-13 Danisco Us Inc Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon
DE102004050410A1 (de) 2004-10-15 2006-06-08 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und dieses codierende Nucleotidsequenz
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
WO2006081825A1 (en) * 2005-02-04 2006-08-10 University Of Aarhus A method for recycling important nutritional elements from waste
CN101460612B (zh) * 2006-04-04 2015-05-06 诺维信公司 肌醇六磷酸酶变体
MX2008012632A (es) 2006-04-04 2008-10-13 Novozymes As Variantes de fitasa.
ES2288126B2 (es) * 2006-06-01 2009-07-06 Universitat De Les Illes Balears Utilizacion de fitato como agente inhibidor de la disolucion de cristales de sales calcicas para la prevencion o tratamiento de la osteoporosis.
WO2008017483A2 (en) * 2006-08-09 2008-02-14 Dsm Ip Assets B.V. Spore surface displays of bioactive molecules
ES2531135T3 (es) 2006-09-21 2015-03-11 Basf Enzymes Llc Fitasas, ácidos nucleicos que las codifican y métodos para su producción y uso
DE102006053059A1 (de) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
EP2011858B1 (de) * 2007-07-06 2012-05-30 Chr. Hansen A/S Gallenresistente, große Mengen Phytase absondernde Bakterienzusammensetzung
BRPI0910457B1 (pt) * 2008-04-18 2020-12-29 Danisco Us Inc. variante de fitase, usos da mesma, composição enzimática, alimento e métodos de produção do mesmo, bem como para redução dos níveis de fósforo em estrume animal
BRPI0923022A2 (pt) 2008-12-19 2015-08-04 Chr Hansen As Composição de bacilos resistentes a bile
CN101492688B (zh) * 2009-01-08 2010-12-08 西南大学 枯草芽孢杆菌中性植酸酶phyC基因
GB0922467D0 (en) 2009-04-24 2010-02-03 Danisco Feed supplement
NZ596459A (en) 2009-05-21 2013-11-29 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them
WO2011031736A2 (en) * 2009-09-14 2011-03-17 The Rockefeller University Methods of identifying anti-inflammatory compounds
US20120201923A1 (en) * 2009-10-22 2012-08-09 Basf Se Synthetic phytase variants
CN106399273B (zh) * 2013-07-24 2019-09-20 东莞泛亚太生物科技有限公司 具有提升的酶活性的植酸酶
TWI478936B (zh) * 2013-07-24 2015-04-01 Genozyme Biotech Inc 具提升酶活性之植酸酶
WO2015070372A1 (zh) * 2013-11-12 2015-05-21 中国农业科学院饲料研究所 一种产生热稳定性改良的植酸酶变体的方法、以及植酸酶变体及其应用
ES2759083T3 (es) * 2014-11-21 2020-05-07 Qingdao Vland Biotech Group Co Ltd Mutantes de fitasa
CN109071615A (zh) 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
BR112018067862A2 (pt) * 2016-03-08 2019-04-16 Basf Enzymes Llc método para melhorar a eficiência de produção de etanol por levedura.
US9605245B1 (en) 2016-06-30 2017-03-28 Fornia BioSoultions, Inc. Phytases and uses thereof
US9528096B1 (en) * 2016-06-30 2016-12-27 Fornia Biosolutions, Inc. Phytases and uses thereof
US10351832B2 (en) 2016-06-30 2019-07-16 Fornia Biosolutions, Inc. Phytases and uses thereof
EP3703661A1 (de) 2017-11-02 2020-09-09 Danisco US Inc. Feste matrixzusammensetzungen mit gesenktem gefrierpunkt für die schmelzgranulation von enzymen
CN108251439B (zh) * 2018-01-11 2021-03-30 山西大学 一种人工改造的耐胰蛋白酶的植酸酶及其制备方法和应用
US20200359656A1 (en) 2018-02-08 2020-11-19 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
CA3098454A1 (en) 2018-04-26 2019-10-31 Danisco Us Inc Method for increasing stability of phytase in a solid composition and a granule composition comprising phosphate and phytase
CA3113377A1 (en) 2018-10-11 2020-04-16 Sanifit Therapeutics S.A. Inositol phosphates for the treatment of ectopic calcification
CN110484455B (zh) * 2019-06-10 2022-05-24 潍坊康地恩生物科技有限公司 一种稳定高产植酸酶的木霉突变菌株
CN111826323A (zh) * 2020-08-05 2020-10-27 福建洛东生物技术有限公司 一种枯草芽孢杆菌及其制剂与应用

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4179337A (en) * 1973-07-20 1979-12-18 Davis Frank F Non-immunogenic polypeptides
JPS6023084B2 (ja) * 1979-07-11 1985-06-05 味の素株式会社 代用血液
US4640835A (en) * 1981-10-30 1987-02-03 Nippon Chemiphar Company, Ltd. Plasminogen activator derivatives
US4496689A (en) * 1983-12-27 1985-01-29 Miles Laboratories, Inc. Covalently attached complex of alpha-1-proteinase inhibitor with a water soluble polymer
US5034506A (en) * 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) * 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
EP0206448B1 (de) * 1985-06-19 1990-11-14 Ajinomoto Co., Inc. Hämoglobin, das an ein Poly(alkenylenoxid) gebunden ist
WO1987005330A1 (en) 1986-03-07 1987-09-11 Michel Louis Eugene Bergh Method for enhancing glycoprotein stability
US5750871A (en) * 1986-05-29 1998-05-12 Calgene, Inc. Transformation and foreign gene expression in Brassica species
US4791192A (en) * 1986-06-26 1988-12-13 Takeda Chemical Industries, Ltd. Chemically modified protein with polyethyleneglycol
US5633076A (en) * 1989-12-01 1997-05-27 Pharming Bv Method of producing a transgenic bovine or transgenic bovine embryo
FR2673643B1 (fr) * 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
US5644048A (en) * 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
ES2268687T3 (es) 1994-04-25 2007-03-16 Dsm Ip Assets B.V. Polipeptidos con actividad fitasa.
US5571706A (en) * 1994-06-17 1996-11-05 The United States Of America As Represented By The Secretary Of Agriculture Plant virus resistance gene and methods
WO1997035016A1 (en) * 1996-03-18 1997-09-25 Novo Nordisk Biotech Inc Polypeptides having phytase activity and nucleic acids encoding same
EP0955362A4 (de) * 1996-04-05 2002-08-14 Kyowa Hakko Kogyo Kk Neuartige phytase und für diese kodierendes gen
US5773269A (en) * 1996-07-26 1998-06-30 Regents Of The University Of Minnesota Fertile transgenic oat plants
PL332375A1 (en) * 1996-09-25 1999-09-13 Kyowa Hakko Kogyo Kk Novel phytases and method of obtaining them
US6039942A (en) * 1996-12-20 2000-03-21 Novo Nordick A/S Phytase polypeptides
US6720014B1 (en) * 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US6855365B2 (en) * 1997-08-13 2005-02-15 Diversa Corporation Recombinant bacterial phytases and uses thereof
US5876997A (en) * 1997-08-13 1999-03-02 Diversa Corporation Phytase
US6183740B1 (en) * 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
GB9917975D0 (en) 1999-07-30 1999-09-29 Smithkline Beecham Biolog Novel compounds
PL354805A1 (en) * 1999-11-18 2004-02-23 Cornell Research Foundation, Inc. Site-directed mutagenesis of escherichia coli
US20030101476A1 (en) * 2000-12-12 2003-05-29 Short Jay M. Recombinant phytases and uses thereof
DE60335195D1 (de) * 2002-08-12 2011-01-13 Danisco Us Inc Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon

Also Published As

Publication number Publication date
DE60335195D1 (de) 2011-01-13
WO2004015084A3 (en) 2004-10-28
EP1546316B1 (de) 2010-12-01
EP1546316A4 (de) 2007-05-02
AU2003258157A8 (en) 2004-02-25
DK1546316T3 (da) 2011-02-14
AU2003258157A1 (en) 2004-02-25
EP1546316A2 (de) 2005-06-29
CN101792749B (zh) 2012-09-05
CN1688690A (zh) 2005-10-26
CN101792749A (zh) 2010-08-04
WO2004015084A2 (en) 2004-02-19
US7968342B2 (en) 2011-06-28
CA2495660A1 (en) 2004-02-19
CN100594239C (zh) 2010-03-17
US20060141562A1 (en) 2006-06-29

Similar Documents

Publication Publication Date Title
ATE490309T1 (de) Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon
WO2003089598A3 (en) Polypeptides having xyloglucanase activity and nucleic acids encoding same
CA2315274A1 (en) Polypeptides having aminopeptidase activity and nucleic acids encoding same
EP1516053A4 (de) Xylanasen, diese codierende nukleinsäuren sowie verfahren zur herstellung und verwendung davon
ATE352626T1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
DE60333201D1 (de) Verfahren zur herstellung von proteinen
DE69731834D1 (de) Agouti verwandtes gen
ATE77104T1 (de) Menge und verfahren zur herstellung rekombinierter erzeugnisse in grosser ausbeute.
NZ591415A (en) Haemophilus influenzae type B
DE60142226D1 (de) Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
ATE414775T1 (de) Polypeptide mit prolyldipeptidylaminopeptidase- aktivität und dafür kodierende nukleinsäuren
EP2284259A3 (de) Proteasen
WO2001029222A3 (en) Polypeptides having phospholipase b activity and nucleic acids encoding same
ATE521695T1 (de) Zirkuläres dna-molekül mit einem konditionalen replikationsursprung, verfahren zur herstellung davon und ihre verwendung in der gentherapie
PL1675956T3 (pl) Minimalna sekwencja DNA działająca jako insulator chromatyny i jej zastosowanie do wyrażania białek
AU7489098A (en) Polypeptides having aminopeptidase activity and nucleic acids encoding same
ATE387497T1 (de) Samenspezifische 7s-alpha-promoter zur expression von genen in pflanzen
DK1157100T3 (da) Oxaloacetathydrolase-defekte svampeværtsceller
ATE492631T1 (de) Verwendung von mutationen zur verbesserung von aspergillus-phytasen
BR0000698A (pt) 15, 15'-dioxigenases de beta, beta-caroteno
WO2003060092A3 (en) Modified fatty acid hydroxylase protein and genes
WO2003029457A1 (fr) Procede de production d'acide amine
ATE384790T1 (de) Uroplakin-ii-promotor aus schwein und verfahren zur herstellung nützlicher proteine unter verwendung des promotors
DE69031996D1 (de) Menschlicher rekombinanter plazentaler ribonuklease-inhibitor und verfahren zur herstellung
AU1526099A (en) Polypeptides having 5-aminolevulinic acid synthase activity and nucleic acids encoding same

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties