ATE492343T1 - Druck-verstärkte extraktion und reinigung - Google Patents

Druck-verstärkte extraktion und reinigung

Info

Publication number
ATE492343T1
ATE492343T1 AT98956405T AT98956405T ATE492343T1 AT E492343 T1 ATE492343 T1 AT E492343T1 AT 98956405 T AT98956405 T AT 98956405T AT 98956405 T AT98956405 T AT 98956405T AT E492343 T1 ATE492343 T1 AT E492343T1
Authority
AT
Austria
Prior art keywords
pressure
phase
purification
partitioning
discovery
Prior art date
Application number
AT98956405T
Other languages
English (en)
Inventor
James Laugharn
Robert Hess
Feng Tao
Original Assignee
Pressure Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/083,651 external-priority patent/US6120985A/en
Application filed by Pressure Biosciences Inc filed Critical Pressure Biosciences Inc
Application granted granted Critical
Publication of ATE492343T1 publication Critical patent/ATE492343T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N2030/009—Extraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N2030/285—Control of physical parameters of the fluid carrier electrically driven carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/522—Physical parameters pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/14—Preparation by elimination of some components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6047—Construction of the column with supporting means; Holders

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Electrochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pens And Brushes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT98956405T 1997-10-31 1998-10-30 Druck-verstärkte extraktion und reinigung ATE492343T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/962,280 US6111096A (en) 1997-10-31 1997-10-31 Nucleic acid isolation and purification
US09/016,062 US6274726B1 (en) 1997-10-31 1998-01-30 Pressure-enhanced extraction and purification
US09/083,651 US6120985A (en) 1997-10-31 1998-05-22 Pressure-enhanced extraction and purification
PCT/US1998/023141 WO1999022868A1 (en) 1997-10-31 1998-10-30 Pressure-enhanced extraction and purification

Publications (1)

Publication Number Publication Date
ATE492343T1 true ATE492343T1 (de) 2011-01-15

Family

ID=25505646

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98956405T ATE492343T1 (de) 1997-10-31 1998-10-30 Druck-verstärkte extraktion und reinigung

Country Status (4)

Country Link
US (3) US6111096A (de)
JP (1) JP2009254384A (de)
AT (1) ATE492343T1 (de)
DE (1) DE69842067D1 (de)

Families Citing this family (128)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6638415B1 (en) * 1995-11-16 2003-10-28 Lifescan, Inc. Antioxidant sensor
US6632349B1 (en) * 1996-11-15 2003-10-14 Lifescan, Inc. Hemoglobin sensor
US7626017B2 (en) * 1997-10-31 2009-12-01 Pressure Biosciences, Inc. Pressure-enhanced extraction and purification
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US7799521B2 (en) * 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
US6780617B2 (en) * 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
DE19836559A1 (de) * 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US6537502B1 (en) * 2000-07-25 2003-03-25 Harvard Apparatus, Inc. Surface coated housing for sample preparation
US7070681B2 (en) * 2001-01-24 2006-07-04 The Board Of Trustees Of The Leland Stanford Junior University Electrokinetic instability micromixer
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
WO2004060278A2 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US7718354B2 (en) 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
WO2002083988A2 (en) * 2001-04-12 2002-10-24 Astra Zeneca Ab Micro-engineered reactors
US6418968B1 (en) 2001-04-20 2002-07-16 Nanostream, Inc. Porous microfluidic valves
JP4633332B2 (ja) * 2001-04-26 2011-02-16 プレッシャー バイオサイエンシズ インコーポレイテッド 試料均質化デバイスおよび生物試料の加工処理方法
KR100552078B1 (ko) * 2001-05-31 2006-02-20 유재천 초소형 구슬을 이용한 미세 밸브 장치 및 그 제어 방법
US20020187557A1 (en) * 2001-06-07 2002-12-12 Hobbs Steven E. Systems and methods for introducing samples into microfluidic devices
US20020186263A1 (en) * 2001-06-07 2002-12-12 Nanostream, Inc. Microfluidic fraction collectors
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
JP4513085B2 (ja) 2001-09-11 2010-07-28 アイキューム インク 試料の容器
WO2003031618A1 (fr) 2001-09-28 2003-04-17 Hitachi, Ltd. Procede de collecte d'acides nucleiques
DE10149251B4 (de) * 2001-10-05 2007-04-19 Sartorius Ag Vorrichtung zur genetischen Immunisierung durch Einbringen von Wirkstoffen in ein Gewebe und Verfahren zur Herstellung einer Injektionslösung
WO2003064605A2 (en) 2002-01-28 2003-08-07 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US7261812B1 (en) 2002-02-13 2007-08-28 Nanostream, Inc. Multi-column separation devices and methods
WO2003070898A2 (en) * 2002-02-19 2003-08-28 Choicepoint Asset Company Selective extraction of dna from groups of cells
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
US7010964B2 (en) * 2002-10-31 2006-03-14 Nanostream, Inc. Pressurized microfluidic devices with optical detection regions
JP4632262B2 (ja) * 2003-02-05 2011-02-16 アイキューム，インク． サンプルの処理
US20040171091A1 (en) * 2003-02-27 2004-09-02 Cell Work, Inc. Standardized evaluation of therapeutic efficacy based on cellular biomarkers
US8501402B2 (en) * 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
US6991253B2 (en) * 2003-04-11 2006-01-31 Delphi Technologies, Inc. Air bag assembly having controlled cushion deployment
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
ES2355899T3 (es) * 2003-05-19 2011-04-01 Brandeis University Procedimientos, kits y dispositivos de tratamiento de ácidos nucleicos.
EP1654361B2 (de) * 2003-07-25 2023-01-18 Life Technologies Corporation Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
US20050032238A1 (en) * 2003-08-07 2005-02-10 Nanostream, Inc. Vented microfluidic separation devices and methods
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8242254B2 (en) 2003-09-11 2012-08-14 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
EP1694869A2 (de) * 2003-11-10 2006-08-30 Investigen, Inc. Verfahren zur herstellung von nukleinsäure für einen nachweis
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
AU2005200670B2 (en) * 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
ES2641832T3 (es) 2004-05-24 2017-11-14 Ibis Biosciences, Inc. Espectrometría de masas con filtración de iones selectiva por establecimiento de umbrales digitales
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
JP4810164B2 (ja) * 2004-09-03 2011-11-09 富士フイルム株式会社 核酸分離精製方法
US7592139B2 (en) 2004-09-24 2009-09-22 Sandia National Laboratories High temperature flow-through device for rapid solubilization and analysis
US20060205040A1 (en) 2005-03-03 2006-09-14 Rangarajan Sampath Compositions for use in identification of adventitious viruses
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
EP1904655A2 (de) 2005-07-21 2008-04-02 Isis Pharmaceuticals, Inc. Verfahren zur raschen identifizierung und quantifizierung von nukleinsäurevarianten
US8921102B2 (en) * 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
KR100785010B1 (ko) * 2006-04-06 2007-12-11 삼성전자주식회사 수소 결합을 이용하여 고체 지지체의 친수성 표면 상에서핵산 정제 방법 및 장치
EP3260556B1 (de) 2006-05-31 2019-07-31 Sequenom, Inc. Verfahren zur nukleinsäureextraktion aus einer probe
EP2029779A4 (de) 2006-06-14 2010-01-20 Living Microsystems Inc Verwendung hoch paralleler snp-genotypisierung zur fötalen diagnose
US20080050739A1 (en) * 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US20080124721A1 (en) * 2006-06-14 2008-05-29 Martin Fuchs Analysis of rare cell-enriched samples
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
EP2589668A1 (de) 2006-06-14 2013-05-08 Verinata Health, Inc Analyse seltener Zellen mittels Probentrennung und DNA-Etiketten
AU2007353877B2 (en) 2006-09-14 2012-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
US20080124777A1 (en) * 2006-11-29 2008-05-29 Canon U.S. Life Sciences, Inc. Method for releasing genetic material from solid phase
US8367397B2 (en) * 2007-02-15 2013-02-05 Honeywell International Inc. Active biochip for nucleic acid analysis
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的ｄｎａ分析法
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
JP5587770B2 (ja) * 2007-06-04 2014-09-10 プレッシャー バイオサイエンシズ インコーポレイテッド 圧力で向上させた、分子の抽出および分配
CA2727246A1 (en) * 2008-05-07 2009-11-12 Pressure Biosciences Inc. Extraction of biomolecular complexes assisted by alternating hydrostatic pressure
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
EP2349549B1 (de) 2008-09-16 2012-07-18 Ibis Biosciences, Inc. Mischpatronen, mischstationen und verwandte ausrüstungen, und system
ES2620012T3 (es) 2008-09-20 2017-06-27 The Board Of Trustees Of The Leland Stanford Junior University Diagnóstico no invasivo de la aneuploidia fetal por secuenciación
WO2010071833A1 (en) 2008-12-19 2010-06-24 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
EP2396803A4 (de) 2009-02-12 2016-10-26 Ibis Biosciences Inc Ionisationssondenanordnungen
US20100216225A1 (en) * 2009-02-25 2010-08-26 Ag-Defense Systems, Inc. Portable microorganism detection unit
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
CA2756463C (en) 2009-03-24 2019-01-22 University Of Chicago Slip chip device and methods
EP2414545B1 (de) 2009-04-03 2017-01-11 Sequenom, Inc. Zusammensetzungen und verfahren für die herstellung von nukleinsäuren
US20100281955A1 (en) * 2009-05-05 2010-11-11 Pressure Biosciences Inc. Microtube and related methods therefor
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
EP2454000A4 (de) 2009-07-17 2016-08-10 Ibis Biosciences Inc Systeme zur identifizierung von biowirkstoffen
KR101626846B1 (ko) * 2009-12-22 2016-06-02 삼성전자주식회사 핵산 분리 방법 및 장치
US8664377B2 (en) * 2009-09-30 2014-03-04 Samsung Electronics Co., Ltd. Method and apparatus for isolating nucleic acids
EP2957641B1 (de) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple-displacement-amplifikation
US8187979B2 (en) * 2009-12-23 2012-05-29 Varian Semiconductor Equipment Associates, Inc. Workpiece patterning with plasma sheath modulation
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
JP5624487B2 (ja) * 2011-01-31 2014-11-12 横河電機株式会社 核酸抽出方法
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
CA2834113C (en) 2011-04-27 2017-11-21 Peking University Methods of nucleic acid liquid-phase extraction and detection
JP6212488B2 (ja) * 2011-07-25 2017-10-11 クヴェッラ コーポレーション 電気的な試料調製のための方法及びデバイス
CA2868555C (en) 2012-03-30 2018-06-12 Jetze Botma Automated selection of microorganisms and identification using maldi
KR101483085B1 (ko) 2012-11-28 2015-01-16 한국전기연구원 액중 전기폭발을 이용한 미세조류 처리 장치
US9556427B2 (en) * 2013-02-27 2017-01-31 Syngenta Participations Ag Methods and compositions for preparation of nucleic acids
EP2983804A4 (de) * 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ionenaustauschreinigung von mrna
EP2971033B8 (de) 2013-03-15 2019-07-10 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
EP2971161B1 (de) 2013-03-15 2018-12-26 ModernaTX, Inc. Nukleinsäurereinigung
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
FR3008713B1 (fr) * 2013-07-18 2016-11-25 Commissariat Energie Atomique Procede d'extraction et de purification d'acides nucleiques et tampons mis en œuvre
WO2015051169A2 (en) 2013-10-02 2015-04-09 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
JP6807232B2 (ja) 2014-05-30 2021-01-06 プレッシャー バイオサイエンシズ インコーポレイテッドＰｒｅｓｓｕｒｅ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． 試料調製デバイスおよび方法
WO2015196128A2 (en) 2014-06-19 2015-12-23 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
CA2955238A1 (en) 2014-07-16 2016-01-21 Moderna Therapeutics, Inc. Circular polynucleotides
CA2962956C (en) * 2014-10-20 2023-09-05 Gen-Probe Incorporated Red blood cell lysis solution
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
SI3350333T1 (sl) 2015-09-17 2022-04-29 ModernaTX,Inc. Polinukleotidi, ki zajemajo stabilizacijsko repno regijo
JP6704920B2 (ja) * 2015-10-14 2020-06-03 アルプスアルパイン株式会社 流路構造体および測定対象液体の測定装置
EP3179243B1 (de) 2015-12-09 2023-09-27 ARKRAY, Inc. Analytisches werkzeug und analytisches system
EP3397763B1 (de) * 2015-12-28 2020-10-28 Koninklijke Philips N.V. Nukleinsäurenreinigungssystem unter verwendung einer einzigen wasch- und eluierungspufferlösung
EP3448998B1 (de) 2016-04-27 2020-06-03 Gen-Probe Incorporated Reagens für blutzelllyse
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
DE102017206155A1 (de) * 2017-04-11 2018-10-11 Robert Bosch Gmbh Desorption von Nukleinsäuren
CN107058075B (zh) * 2017-06-20 2023-10-20 商丘师范学院 一种植物细胞原生质体纯化仪及纯化方法
CN113365730A (zh) 2018-11-28 2021-09-07 V&P科学有限公司 用于混合、悬浮颗粒、抽样、洗涤磁珠和浓缩分析物的旋转容器系统和方法
EP3999205A4 (de) * 2019-07-15 2023-08-16 Lexagene, Inc. Probenvorbereitungskasseten und vorrichtungen
TWI911320B (zh) 2020-11-20 2026-01-11 美商伊路米納有限公司 致動設備
WO2022158402A1 (ja) * 2021-01-25 2022-07-28 横河電機株式会社 核酸配列計測方法
CN116790578B (zh) * 2023-08-22 2023-12-12 赛奥斯博生物科技(北京)有限公司 一种基于碱裂解法快速纯化质粒的生产工艺
WO2025166356A1 (en) * 2024-02-01 2025-08-07 Fmrk Diagnostic Technologies Incorporated Specimen collection containers and related transfer and/or enrichment systems and methods

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3609252A (en) * 1967-01-23 1971-09-28 Texas Instruments Inc Transducer apparatus and system utilizing insulated gate semiconductor field effect devices
US4323439A (en) 1979-12-31 1982-04-06 The Regents Of The University Of California Method and apparatus for dynamic equilibrium electrophoresis
GB8311905D0 (en) 1983-04-29 1983-06-02 Cox R A Isolation and detection of nucleotide sequence
US5346999A (en) 1985-01-18 1994-09-13 Applied Biosystems, Inc. Method of nucleic acid extraction
US5003047A (en) * 1989-01-10 1991-03-26 Massachusetts Institute Of Technology Method for purifying biologically active ligate
JPH02255059A (ja) * 1989-03-25 1990-10-15 Chuetsu Koubo Kogyo Kk 酵母エキスの製造法
IT1240870B (it) 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
JP2873971B2 (ja) * 1990-03-02 1999-03-24 オリエンタル酵母工業株式会社 微生物内容物の漏出方法
JPH0779658B2 (ja) * 1990-03-16 1995-08-30 株式会社ジャパンエナジー ねぎ属植物の処理方法
US5605839A (en) 1990-04-11 1997-02-25 Ludwig Institute For Cancer Research Methods and apparatus for use in sequential chemical reactions
JPH04349892A (ja) * 1991-05-21 1992-12-04 Toshiba Corp 核酸の単離方法
DE4319506A1 (de) 1993-06-12 1994-12-15 Boehringer Mannheim Gmbh Verfahren zum Nachweis metabolisch markierter DNA
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5438127A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
WO1995034569A1 (de) * 1994-06-14 1995-12-21 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
JP2992737B2 (ja) * 1994-06-27 1999-12-20 備前化成株式会社 凍結細胞膜破壊による動植物エキスの抽出法
DE4431280A1 (de) * 1994-09-02 1996-03-07 Hoechst Ag Verfahren zur Isolierung und Reinigung von nukleotidaktivierten Zuckern aus biologischen Quellen
DE4441150A1 (de) * 1994-11-18 1996-05-23 Teves Gmbh Alfred Druckregelventil
WO1996027432A1 (en) * 1995-03-07 1996-09-12 Biomolecular Assays, Inc. Pressure cycling reactor
IL113935A (en) * 1995-05-31 1999-09-22 Imi Tami Institute Research Process for the recovery of carotenoid pigment from algae
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
JPH0919284A (ja) * 1995-07-04 1997-01-21 Oriental Yeast Co Ltd 分裂酵母倍数株の取得方法
WO1997031105A1 (en) 1996-02-25 1997-08-28 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
DE19610354C1 (de) * 1996-03-15 1997-11-20 Innova Gmbh Vorrichtung, Verfahren und Einrichtung zur Isolierung von Nukleinsäuren
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography

Also Published As

Publication number Publication date
US6274726B1 (en) 2001-08-14
DE69842067D1 (de) 2011-02-03
JP2009254384A (ja) 2009-11-05
US20020016450A1 (en) 2002-02-07
US6111096A (en) 2000-08-29

Similar Documents

Publication Publication Date Title
DE69842067D1 (de) Druck-verstärkte extraktion und reinigung
Wang et al. Movable contactless-conductivity detector for microchip capillary electrophoresis
Eie et al. Electromembrane extraction of polar substances–status and perspectives
Wang et al. Measurements of chemical warfare agent degradation products using an electrophoresis microchip with contactless conductivity detector
US6120985A (en) Pressure-enhanced extraction and purification
AU5131585A (en) Sheet-like diagnostic device
NO914948L (no) Innretning for elektroaktivering av fluider og preparatersom bestaar av elektroaktiverte fluider
DE69811886D1 (de) Biosensor mit ionendurchlässige kanäle aufweisender lipidmembran
ATE217324T1 (de) Verfahren zur quantitativen selektiven entfernung oder präperativen gewinnung von tumor-nekrose- faktor (tnf) und lipopolysacchariden (lps) aus wässrigen flüssigkeiten
WO1998038510A3 (en) Laboratory in a disk
MX9700845A (es) Aparato y metodo para llevar a cabo manipulaciones de microfluidos para analisis y sintesis quimicos.
WO2004003538A3 (de) Multi-elektroden anordnung zur detektion eines analyten
Lin et al. A poly-methylmethacrylate electrophoresis microchip with sample preconcentrator
Walhagen et al. Capillary electrochromatography analysis of hormonal cyclic and linear peptides
WO2001077658A3 (en) Cassette for bufferless gel electrophoresis
Pantůčková et al. Sensitivity enhancement in direct coupling of supported liquid membrane extractions to capillary electrophoresis by means of transient isotachophoresis and large electrokinetic injections
Zhu et al. Integrated bare narrow capillary–hydrodynamic chromatographic system for free-solution DNA separation at the single-molecule level
Mersal et al. Development of monolithic enzymatic reactors in glass microchips for the quantitative determination of enzyme substrates using the example of glucose determination via immobilized glucose oxidase
ATE202498T1 (de) Selbsterhitzendes, elektrisch leitfähiges querstromsorptionssystem
SE9703578D0 (sv) Cassette for electrophoresis system
GB0030708D0 (en) Single channel proteomics concepts
AUPR222400A0 (en) Apparatus and method for separation of molecules and movementof fluids
US20050006240A1 (en) Field generating membrane electrode
Baba et al. High performance separations of nucleic acids using capillary electrophoresis and high‐performance liquid chromatography
Erijman et al. High pressure electrophoresis in narrow bore glass tubes: One‐and two‐dimensional separations of protein subunits

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties