ATE494548T1 - Reinigung, charakterisierung und wiederherstellung einer ubiquitin-e3-ligase - Google Patents
Reinigung, charakterisierung und wiederherstellung einer ubiquitin-e3-ligaseInfo
- Publication number
- ATE494548T1 ATE494548T1 AT06784518T AT06784518T ATE494548T1 AT E494548 T1 ATE494548 T1 AT E494548T1 AT 06784518 T AT06784518 T AT 06784518T AT 06784518 T AT06784518 T AT 06784518T AT E494548 T1 ATE494548 T1 AT E494548T1
- Authority
- AT
- Austria
- Prior art keywords
- ubiquitin
- complex
- methods
- ligase
- characterization
- Prior art date
Links
- 102000044159 Ubiquitin Human genes 0.000 title abstract 3
- 108090000848 Ubiquitin Proteins 0.000 title abstract 3
- 102000003960 Ligases Human genes 0.000 title 1
- 108090000364 Ligases Proteins 0.000 title 1
- 238000012512 characterization method Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 238000011084 recovery Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 108010083111 Ubiquitin-Protein Ligases Proteins 0.000 abstract 2
- 102000006275 Ubiquitin-Protein Ligases Human genes 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 102100026681 Chromobox protein homolog 8 Human genes 0.000 abstract 1
- 101000910841 Homo sapiens Chromobox protein homolog 8 Proteins 0.000 abstract 1
- 101000583616 Homo sapiens Polyhomeotic-like protein 2 Proteins 0.000 abstract 1
- 101100058550 Mus musculus Bmi1 gene Proteins 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 102100030903 Polyhomeotic-like protein 2 Human genes 0.000 abstract 1
- 101150007742 RING1 gene Proteins 0.000 abstract 1
- 208000035217 Ring chromosome 1 syndrome Diseases 0.000 abstract 1
- 208000032825 Ring chromosome 2 syndrome Diseases 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 230000034512 ubiquitination Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/9015—Ligases (6)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68676905P | 2005-06-02 | 2005-06-02 | |
| US75056405P | 2005-12-15 | 2005-12-15 | |
| PCT/US2006/021159 WO2006130720A2 (en) | 2005-06-02 | 2006-06-01 | Purification, characterization and reconstitution of a ubiquitin e3 ligase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE494548T1 true ATE494548T1 (de) | 2011-01-15 |
Family
ID=37482290
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06784518T ATE494548T1 (de) | 2005-06-02 | 2006-06-01 | Reinigung, charakterisierung und wiederherstellung einer ubiquitin-e3-ligase |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7923219B2 (de) |
| EP (1) | EP1891434B1 (de) |
| AT (1) | ATE494548T1 (de) |
| DE (1) | DE602006019423D1 (de) |
| WO (1) | WO2006130720A2 (de) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011160206A1 (en) | 2010-06-23 | 2011-12-29 | Morin Ryan D | Biomarkers for non-hodgkin lymphomas and uses thereof |
| US9175331B2 (en) | 2010-09-10 | 2015-11-03 | Epizyme, Inc. | Inhibitors of human EZH2, and methods of use thereof |
| BR112013005806B1 (pt) | 2010-09-10 | 2022-05-10 | Epizyme, Inc | Métodos para detectar se um indivíduo é um candidato para o tratamento com ou responsivo a um inibidor de ezh2 e usos terapêuticos do dito inibidor de ezh2 |
| TW201733984A (zh) | 2011-04-13 | 2017-10-01 | 雅酶股份有限公司 | 經取代之苯化合物 |
| JO3363B1 (ar) | 2011-04-13 | 2019-03-13 | Epizyme Inc | مركبات بنزين مستبدلة بأريل أو أريل غير متجانس |
| US9394283B2 (en) | 2012-04-13 | 2016-07-19 | Epizyme, Inc. | Salt form of a human histone methyltransferase EZH2 inhibitor |
| PE20150887A1 (es) | 2012-10-15 | 2015-06-04 | Epizyme Inc | Compuestos de benceno sustituidos |
| EP2984101B1 (de) * | 2013-04-05 | 2020-08-19 | Qiagen Sciences, LLC | Kits und verfahren zur isolierung von protein aus biologischen und umweltproben |
| US9464311B2 (en) | 2013-05-02 | 2016-10-11 | E3X Bio, Inc. | Method for identifying modulators of ubiquitin ligases |
| KR102142177B1 (ko) | 2013-10-16 | 2020-08-07 | 에피자임, 인코포레이티드 | Ezh2 억제용 하이드로클로라이드 염 형태 |
| US11242518B2 (en) | 2015-09-04 | 2022-02-08 | QIAGEN Sciences, LLP | Methods for co-isolation of nucleic acids and proteins |
| WO2018132825A2 (en) * | 2017-01-16 | 2018-07-19 | Memorial Sloan Kettering Cancer Center | Treatment of sarcoma |
| EP3784796A1 (de) | 2018-04-24 | 2021-03-03 | Qiagen Sciences, LLC | Isolierung von nukleinsäuren und entfernung von inhibitoren aus komplexen proben |
| WO2020264348A1 (en) * | 2019-06-27 | 2020-12-30 | Board Of Regents, The University Of Texas System | Inhibitors of prc1 for treatment of cancer |
| KR102288716B1 (ko) * | 2019-10-30 | 2021-08-12 | 경상남도 | 항비만 효능을 갖는 펩타이드 및 이의 용도 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6740495B1 (en) * | 2000-04-03 | 2004-05-25 | Rigel Pharmaceuticals, Inc. | Ubiquitin ligase assay |
| US6919184B2 (en) * | 2000-04-03 | 2005-07-19 | Rigel Pharmaceuticals, Inc. | Assays for identifying ubiquitin agents and for identifying agents that modify the activity of ubiquitin agents |
| US7608682B2 (en) * | 2002-10-30 | 2009-10-27 | Meso Scale Technologies Llc | Substrates of N-end rule ubiquitylation and methods for measuring the ubiquitylation of these substrates |
| GB0228900D0 (en) | 2002-12-11 | 2003-01-15 | Ml Lab Plc | Cancer Immunotherapy |
| EP1651595A2 (de) * | 2003-05-30 | 2006-05-03 | Rigel Pharmaceuticals, Inc. | Ubiquitinligaseinhibitoren |
-
2006
- 2006-06-01 US US11/915,610 patent/US7923219B2/en not_active Expired - Fee Related
- 2006-06-01 AT AT06784518T patent/ATE494548T1/de not_active IP Right Cessation
- 2006-06-01 WO PCT/US2006/021159 patent/WO2006130720A2/en not_active Ceased
- 2006-06-01 DE DE602006019423T patent/DE602006019423D1/de active Active
- 2006-06-01 EP EP06784518A patent/EP1891434B1/de not_active Not-in-force
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006130720A3 (en) | 2007-04-26 |
| WO2006130720A2 (en) | 2006-12-07 |
| US20080248458A1 (en) | 2008-10-09 |
| DE602006019423D1 (de) | 2011-02-17 |
| EP1891434A2 (de) | 2008-02-27 |
| EP1891434B1 (de) | 2011-01-05 |
| EP1891434A4 (de) | 2008-08-27 |
| US7923219B2 (en) | 2011-04-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |