ATE509120T1 - Fusionsproteine zur detektion von analyten - Google Patents

Fusionsproteine zur detektion von analyten

Info

Publication number
ATE509120T1
ATE509120T1 AT02767487T AT02767487T ATE509120T1 AT E509120 T1 ATE509120 T1 AT E509120T1 AT 02767487 T AT02767487 T AT 02767487T AT 02767487 T AT02767487 T AT 02767487T AT E509120 T1 ATE509120 T1 AT E509120T1
Authority
AT
Austria
Prior art keywords
detection
portions
fusion proteins
protein
detecting analytes
Prior art date
Application number
AT02767487T
Other languages
English (en)
Inventor
Wolf-Bernd Frommer
Marcus Fehr
Sylvie Lalonde
Original Assignee
Carnegie Inst Of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carnegie Inst Of Washington filed Critical Carnegie Inst Of Washington
Application granted granted Critical
Publication of ATE509120T1 publication Critical patent/ATE509120T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT02767487T 2001-09-18 2002-09-13 Fusionsproteine zur detektion von analyten ATE509120T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01122301 2001-09-18
EP02007373 2002-04-08
PCT/EP2002/010340 WO2003025220A2 (en) 2001-09-18 2002-09-13 Fusion proteins useful for detecting analytes

Publications (1)

Publication Number Publication Date
ATE509120T1 true ATE509120T1 (de) 2011-05-15

Family

ID=26076716

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02767487T ATE509120T1 (de) 2001-09-18 2002-09-13 Fusionsproteine zur detektion von analyten

Country Status (4)

Country Link
US (1) US20070136825A1 (de)
EP (2) EP2284195A1 (de)
AT (1) ATE509120T1 (de)
WO (1) WO2003025220A2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003284127A1 (en) 2002-10-09 2004-05-04 The Trustees Of Boston University Nucleic acid supported protein complementation
US20050112685A1 (en) * 2003-11-26 2005-05-26 Amiss Terry J. Compositions and methods for measuring analyte concentrations
KR20070040329A (ko) * 2004-03-17 2007-04-16 유니버시티 오브 하와이 센서 구조물 및 검출방법
EP1786834B1 (de) * 2004-07-14 2016-02-17 Glusense Ltd. Implantierbare Stromquellen und -Sensoren
EP1805200B1 (de) * 2004-10-14 2012-01-04 Carnegie Institution Of Washington Neurotransmittersensoren und verfahren zu ihrer verwendung
AU2005295656B2 (en) * 2004-10-14 2011-08-04 Carnegie Institution Of Washington Development of sensitive FRET sensors and methods of using the same
EP1856138A4 (de) * 2005-03-04 2008-12-10 Carnegie Inst Of Washington Umweltstabile sensoren und verfahren zu deren anwendung
KR100739529B1 (ko) 2005-07-25 2007-07-13 한국생명공학연구원 세포내 맥아당의 정량적 측정을 위한 맥아당 바이오센서
ATE489397T1 (de) 2005-10-14 2010-12-15 Carnegie Inst Of Washington Saccharose-biosensoren und verwendungsverfahren dafür
CA2625327A1 (en) * 2005-10-14 2007-04-19 Carnegie Institution Of Washington Phosphate biosensors and methods of using the same
CA2625169A1 (en) * 2005-10-14 2007-04-26 Carnegie Institution Of Washington Methods of reducing repeat-induced silencing of transgene expression and improved fluorescent biosensors
CA2627413A1 (en) * 2005-10-27 2007-05-03 The Trustees Of Boston University Activated split-polypeptides and methods for their production and use
US20120122115A1 (en) * 2006-04-25 2012-05-17 Sayre Richard T Bacterial quorum sensing biosensor
EP2097507B1 (de) * 2006-12-14 2015-05-06 Georgia State University Research Foundation, Inc. Analytsensoren und verwendung davon für den nachweis von analytaktivität
DK2989975T3 (en) 2007-02-06 2018-09-24 Medtronic Minimed Inc OPTICAL SYSTEMS AND PROCEDURES FOR RATIOMETRIC MEASUREMENT OF BLOOD GLUCOSE CONCENTRATION
US8088097B2 (en) 2007-11-21 2012-01-03 Glumetrics, Inc. Use of an equilibrium intravascular sensor to achieve tight glycemic control
EP2162057A1 (de) 2007-05-10 2010-03-17 Glumetrics, Inc. Verschleissbeständiger gleichgewichtsfluoreszenzsensor für intravaskuläre echtzeit-glukosemessung
AU2008265231B2 (en) * 2007-06-20 2014-05-01 Basf Se Synthetic repetitive proteins, the production and use thereof
US8465981B2 (en) * 2007-08-06 2013-06-18 University Of Kentucky Research Foundation Polypeptides, systems, and methods useful for detecting glucose
WO2009021026A1 (en) * 2007-08-06 2009-02-12 University Of Kentucky Research Foundation Semi-synthetic antibodies as recognition elements
US20120028265A1 (en) * 2007-08-10 2012-02-02 Thijs Kaper Methods of using ret nanosensors
US8742204B2 (en) * 2007-08-29 2014-06-03 Plant Sensory Systems, Llc Metabolic regulators
CA2698076A1 (en) * 2007-08-29 2009-03-05 Plant Sensory System, Llc. Metabolic regulators
ITTO20070687A1 (it) * 2007-10-01 2009-04-02 Consiglio Nazionale Ricerche Nuovo mutante di gfp sensibile al ph e alla concentrazione di anioni, proteina chimerica comprendente tale mutante e procedimento di determinazione combinata del ph e della concentrazione di anioni
EP3072963B1 (de) * 2007-10-18 2020-04-01 Cell Signaling Technology, Inc. Translokation und mutations-ros-kinase bei menschlichem nicht-kleinzelligem bronchialkarzinom
US8663632B1 (en) 2008-03-13 2014-03-04 University Of Kentucky Research Foundation Compositions and methods for selectively targeting cancer cells using a thiaminase compound
WO2010066113A1 (zh) * 2008-12-11 2010-06-17 国立大学法人东京大学 拆分荧光蛋白的融合蛋白组合、其表达载体，稳定表达哺乳动物细胞系及筛选方法
JP2013506503A (ja) 2009-09-30 2013-02-28 グルメトリクス， インコーポレイテッド 抗血栓性コーティングを備えたセンサー
US8741591B2 (en) * 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
US8467843B2 (en) 2009-11-04 2013-06-18 Glumetrics, Inc. Optical sensor configuration for ratiometric correction of blood glucose measurement
WO2011113020A1 (en) * 2010-03-11 2011-09-15 Glumetrics, Inc. Measurement devices and methods for measuring analyte concentration incorporating temperature and ph correction
US20120076736A1 (en) * 2010-09-17 2012-03-29 Rochester Institute Of Technology Host supported genetic biosensors
JP6158087B2 (ja) 2010-10-19 2017-07-05 ジョージア ステート ユニバーシティ リサーチ ファウンデーション インコーポレイテッド 分析物センサー、そのようなセンサーを調製および使用するための方法、および分析物の活性を検出する方法
US9037205B2 (en) 2011-06-30 2015-05-19 Glusense, Ltd Implantable optical glucose sensing
WO2013154587A1 (en) * 2012-04-13 2013-10-17 Centro De Estudios Cientificos De Valdivia Genetically encoded probe for quantification of lactate, and methods for quantifying metabolic rates and lactate transport
WO2016054532A1 (en) * 2014-10-03 2016-04-07 President And Fellows Of Harvard College Self-labeling nucleic acids and methods of use
EP3206567A1 (de) 2014-10-13 2017-08-23 Glusense, Ltd. Analytmessvorrichtung
JP6535483B2 (ja) * 2015-03-02 2019-06-26 国立研究開発法人農業・食品産業技術総合研究機構 幼若ホルモンセンサー
CA3005831A1 (en) * 2015-11-20 2017-05-26 Duke University Glucose biosensors and uses thereof
EP3445872A1 (de) 2016-04-20 2019-02-27 Glusense Ltd. Fret-basierte glucosedetektionsmoleküle
US11951186B2 (en) 2019-10-25 2024-04-09 Willow Laboratories, Inc. Indicator compounds, devices comprising indicator compounds, and methods of making and using the same
WO2021158973A1 (en) 2020-02-06 2021-08-12 Trustees Of Boston University Enzyme-based electrochemical nicotine biosensor
WO2021221752A2 (en) 2020-02-06 2021-11-04 Trustees Of Boston University High throughput assay for identifying microbial redox enzymes
IL297237B2 (en) 2020-04-17 2025-08-01 Regeneron Pharma Detection assays for coronavirus neutralizing antibodies
CN117405502B (zh) * 2023-12-12 2024-02-13 山东海奥斯生物科技股份有限公司 一种植物蛋白膜强度自动检测装置

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
ATE115999T1 (de) 1987-12-15 1995-01-15 Gene Shears Pty Ltd Ribozyme.
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5792608A (en) 1991-12-12 1998-08-11 Gilead Sciences, Inc. Nuclease stable and binding competent oligomers and methods for their use
EP0903408A3 (de) 1992-08-21 2005-11-02 Biogen, Inc. Tat-derivate Transport-Polypeptide
DK0733059T3 (da) 1993-12-09 2000-10-16 Univ Jefferson Forbindelser og fremgangsmåder til site-rettede mutationer i eukaryote celler
US5525711A (en) 1994-05-18 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pteridine nucleotide analogs as fluorescent DNA probes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
CA2283629C (en) 1997-03-14 2010-08-03 Roger Y. Tsien Fluorescent protein sensors for detection of analytes
WO1999066324A2 (en) 1998-06-16 1999-12-23 Biosignal Packard Inc. A bioluminescence resonance energy transfer (bret) system and its use
DE69941085D1 (de) * 1998-07-17 2009-08-20 Univ Maryland Manipulierte proteine zum analyten nachweis
WO2000034321A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034322A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034326A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034324A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034318A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034323A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034319A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000049183A1 (en) * 1999-02-17 2000-08-24 Clontech Laboratories, Inc. FLUORESCENCE RESONANCE ENERGY TRANSFER DETECTION OF cAMP IN LIVING CELLS USING GFP VARIANTS
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
KR100739529B1 (ko) * 2005-07-25 2007-07-13 한국생명공학연구원 세포내 맥아당의 정량적 측정을 위한 맥아당 바이오센서

Also Published As

Publication number Publication date
US20070136825A1 (en) 2007-06-14
EP1427856A2 (de) 2004-06-16
EP1427856B1 (de) 2011-05-11
WO2003025220A3 (en) 2003-12-11
WO2003025220A2 (en) 2003-03-27
EP2284195A9 (de) 2013-03-27
EP2284195A1 (de) 2011-02-16

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