ATE509120T1 - Fusionsproteine zur detektion von analyten - Google Patents

Fusionsproteine zur detektion von analyten

Info

Publication number
ATE509120T1
ATE509120T1 AT02767487T AT02767487T ATE509120T1 AT E509120 T1 ATE509120 T1 AT E509120T1 AT 02767487 T AT02767487 T AT 02767487T AT 02767487 T AT02767487 T AT 02767487T AT E509120 T1 ATE509120 T1 AT E509120T1
Authority
AT
Austria
Prior art keywords
detection
portions
fusion proteins
protein
detecting analytes
Prior art date
Application number
AT02767487T
Other languages
English (en)
Inventor
Wolf-Bernd Frommer
Marcus Fehr
Sylvie Lalonde
Original Assignee
Carnegie Inst Of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carnegie Inst Of Washington filed Critical Carnegie Inst Of Washington
Application granted granted Critical
Publication of ATE509120T1 publication Critical patent/ATE509120T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT02767487T 2001-09-18 2002-09-13 Fusionsproteine zur detektion von analyten ATE509120T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01122301 2001-09-18
EP02007373 2002-04-08
PCT/EP2002/010340 WO2003025220A2 (en) 2001-09-18 2002-09-13 Fusion proteins useful for detecting analytes

Publications (1)

Publication Number Publication Date
ATE509120T1 true ATE509120T1 (de) 2011-05-15

Family

ID=26076716

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02767487T ATE509120T1 (de) 2001-09-18 2002-09-13 Fusionsproteine zur detektion von analyten

Country Status (4)

Country Link
US (1) US20070136825A1 (de)
EP (2) EP2284195A1 (de)
AT (1) ATE509120T1 (de)
WO (1) WO2003025220A2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7662554B2 (en) 2002-10-09 2010-02-16 The Trustees Of Boston University Nucleic acid supported protein complementation
US20050112685A1 (en) * 2003-11-26 2005-05-26 Amiss Terry J. Compositions and methods for measuring analyte concentrations
JP2007529747A (ja) 2004-03-17 2007-10-25 ユニバーシティ オブ ハワイ センサ構造物及び検出方法
WO2006006166A2 (en) 2004-07-14 2006-01-19 Glusense, Ltd. Implantable power sources and sensors
EP1815246A4 (de) * 2004-10-14 2009-12-16 Carnegie Inst Of Washington Entwicklung von empfindlichen fret-sensoren und verwendungsverfahren dafür
WO2006044611A2 (en) * 2004-10-14 2006-04-27 Carnegie Institution Of Washington Neurotransmitter sensors and methods of using the same
CA2599852A1 (en) * 2005-03-04 2006-09-14 Carnegie Institution Of Washington Environmentally stable sensors and methods of using the same
KR100739529B1 (ko) * 2005-07-25 2007-07-13 한국생명공학연구원 세포내 맥아당의 정량적 측정을 위한 맥아당 바이오센서
CA2625169A1 (en) * 2005-10-14 2007-04-26 Carnegie Institution Of Washington Methods of reducing repeat-induced silencing of transgene expression and improved fluorescent biosensors
AU2005337445A1 (en) * 2005-10-14 2007-04-26 Carnegie Institution Of Washington Sucrose biosensors and methods of using the same
CA2625327A1 (en) 2005-10-14 2007-04-19 Carnegie Institution Of Washington Phosphate biosensors and methods of using the same
JP2009515510A (ja) * 2005-10-27 2009-04-16 トラスティーズ オブ ボストン ユニバーシティ 活性化分割ポリペプチドならびにその生成法および使用
US20120122115A1 (en) * 2006-04-25 2012-05-17 Sayre Richard T Bacterial quorum sensing biosensor
US8420327B2 (en) 2006-12-14 2013-04-16 Georgia State University Research Foundation Analyte sensors, methods for preparing and using such sensors, and methods of detecting analyte activity
AU2008213677A1 (en) 2007-02-06 2008-08-14 Glumetrics, Inc. Optical systems and methods for rationmetric measurement of blood glucose concentration
JP5517919B2 (ja) 2007-05-10 2014-06-11 グルメトリクス、 インク. 即時血管内グルコース測定のための平衡非消費蛍光センサー
WO2008155304A1 (de) * 2007-06-20 2008-12-24 Basf Se Synthetische repetitive proteine, deren herstellung und verwendung
WO2009021052A1 (en) * 2007-08-06 2009-02-12 University Of Kentucky Research Foundation Polypeptides, systems, and methods useful for detecting glucose
US20110229415A1 (en) * 2007-08-06 2011-09-22 Sylvia Daunert Semi-synthetic antibodies as recognition elements
WO2009023153A1 (en) * 2007-08-10 2009-02-19 Carnegie Institution Of Washington Methods of using ret nanosensors
US20090061519A1 (en) * 2007-08-29 2009-03-05 Plant Sensory Systems, Llc Metabolic regulators
US8742204B2 (en) * 2007-08-29 2014-06-03 Plant Sensory Systems, Llc Metabolic regulators
ITTO20070687A1 (it) * 2007-10-01 2009-04-02 Consiglio Nazionale Ricerche Nuovo mutante di gfp sensibile al ph e alla concentrazione di anioni, proteina chimerica comprendente tale mutante e procedimento di determinazione combinata del ph e della concentrazione di anioni
EP3741851A1 (de) 2007-10-18 2020-11-25 Cell Signaling Technology, Inc. Translokation und mutante ros-kinase in humanem nichtkleinzelligem lungenkarzinom
WO2009067626A1 (en) 2007-11-21 2009-05-28 Glumetrics, Inc. Use of an equilibrium intravascular sensor to achieve tight glycemic control
US8663632B1 (en) 2008-03-13 2014-03-04 University Of Kentucky Research Foundation Compositions and methods for selectively targeting cancer cells using a thiaminase compound
WO2010066113A1 (zh) * 2008-12-11 2010-06-17 国立大学法人东京大学 拆分荧光蛋白的融合蛋白组合、其表达载体,稳定表达哺乳动物细胞系及筛选方法
EP2483679A4 (de) 2009-09-30 2013-04-24 Glumetrics Inc Sensoren mit thrombenresistenter beschichtung
US8741591B2 (en) * 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
US8467843B2 (en) 2009-11-04 2013-06-18 Glumetrics, Inc. Optical sensor configuration for ratiometric correction of blood glucose measurement
EP2545373B1 (de) * 2010-03-11 2022-08-24 Medtronic Minimed, Inc. Messung von analytkonzentrationen einschliesslich temperatur- und ph-korrektur
US20120076736A1 (en) * 2010-09-17 2012-03-29 Rochester Institute Of Technology Host supported genetic biosensors
AU2011317082B2 (en) 2010-10-19 2016-11-03 Georgia State University Research Foundation, Inc. Analyte sensors, methods for preparing and using such sensors, and methods of detecting analyte activity
US9037205B2 (en) 2011-06-30 2015-05-19 Glusense, Ltd Implantable optical glucose sensing
US10466233B2 (en) * 2012-04-13 2019-11-05 Centro De Estudios Cientificos De Valdivia Genetically encoded probe for quantification of lactate and methods for quantifying metabolic rates and lactate transport
WO2016054532A1 (en) * 2014-10-03 2016-04-07 President And Fellows Of Harvard College Self-labeling nucleic acids and methods of use
WO2016059635A1 (en) 2014-10-13 2016-04-21 Glusense Ltd. Analyte-sensing device
JP6535483B2 (ja) * 2015-03-02 2019-06-26 国立研究開発法人農業・食品産業技術総合研究機構 幼若ホルモンセンサー
CA3005831A1 (en) * 2015-11-20 2017-05-26 Duke University Glucose biosensors and uses thereof
WO2017183030A1 (en) 2016-04-20 2017-10-26 Glusense Ltd. Fret-based glucose-detection molecules
WO2021081404A1 (en) * 2019-10-25 2021-04-29 Cercacor Laboratories, Inc. Indicator compounds, devices comprising indicator compounds, and methods of making and using the same
US11536685B2 (en) 2020-02-06 2022-12-27 Trustees Of Boston University High throughput assay for identifying microbial redox enzymes
US11331020B2 (en) 2020-02-06 2022-05-17 Trustees Of Boston University Enzyme-based electrochemical nicotine biosensor
IL297237B2 (en) 2020-04-17 2025-08-01 Regeneron Pharma Detection assays for coronavirus neutralizing antibodies
CN117405502B (zh) * 2023-12-12 2024-02-13 山东海奥斯生物科技股份有限公司 一种植物蛋白膜强度自动检测装置

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
AU632993B2 (en) 1987-12-15 1993-01-21 Gene Shears Pty. Limited Ribozymes
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5792608A (en) 1991-12-12 1998-08-11 Gilead Sciences, Inc. Nuclease stable and binding competent oligomers and methods for their use
EP0903408A3 (de) 1992-08-21 2005-11-02 Biogen, Inc. Tat-derivate Transport-Polypeptide
CN1048254C (zh) 1993-12-09 2000-01-12 托马斯杰弗逊大学 用于将预定的改变引入靶基因中的化合物
US5525711A (en) 1994-05-18 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pteridine nucleotide analogs as fluorescent DNA probes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
AU6702398A (en) 1997-03-14 1998-09-29 Regents Of The University Of California, The Fluorescent protein sensors for detection of analytes
EP1088233B9 (de) 1998-06-16 2006-05-17 PerkinElmer BioSignal Inc. Biolumineszentes resonanz-energie-transfersystem (bret) und seine anwendung
AU759888B2 (en) * 1998-07-17 2003-05-01 University Of Maryland At Baltimore Engineered proteins for analyte sensing
WO2000034326A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034318A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034322A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034323A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
ATE502116T1 (de) 1998-12-11 2011-04-15 Clontech Lab Inc Fluoreszierende proteine aus nicht- biolumineszenten arten der klasse anthozoa, gene die diese proteine kodieren und deren verwendungen
WO2000034324A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034321A1 (en) 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000049183A1 (en) * 1999-02-17 2000-08-24 Clontech Laboratories, Inc. FLUORESCENCE RESONANCE ENERGY TRANSFER DETECTION OF cAMP IN LIVING CELLS USING GFP VARIANTS
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
KR100739529B1 (ko) * 2005-07-25 2007-07-13 한국생명공학연구원 세포내 맥아당의 정량적 측정을 위한 맥아당 바이오센서

Also Published As

Publication number Publication date
WO2003025220A3 (en) 2003-12-11
EP2284195A9 (de) 2013-03-27
EP1427856A2 (de) 2004-06-16
US20070136825A1 (en) 2007-06-14
EP1427856B1 (de) 2011-05-11
WO2003025220A2 (en) 2003-03-27
EP2284195A1 (de) 2011-02-16

Similar Documents

Publication Publication Date Title
DE60238493D1 (de) Is von seltenen zellen verwendete markierte zellen
CY1109554T1 (el) Μεθοδοι και αντιδραστηρια για την ανιχνευση ενδοτοξινης
ATE237133T1 (de) Assays zur bestimmung von proteinfragmenten in biologischen medien
DE69823985D1 (de) Nachweis der krankheitsbedingten konformation eines proteins
DE60211651D1 (de) Proteom-analyseverfahren
DK1579222T3 (da) Ikke-kompetitivt immunassay til små analytter
CY1109480T1 (el) ΠΡΩΤΕΪΝΕΣ ΣΥΝΔΕΣΗΣ Gag
ATE446511T1 (de) Neue fusionsproteine und molekulare bindungsassays
HUT77049A (hu) Katepszin-02-fehérjék, -nukleinsavak és -ellenanyagok
DE60013320D1 (de) Analytassay-vorrichtung mit vorabsorbieren-zone
BR9812783A (pt) "sistemas de detecção para o registro de interações e as relações funcionais de proteìnas"
EA200300880A1 (ru) Способ детекции нуклеотидного полиморфизма
DE60044420D1 (de) Enzym-Protein-Komplex
DE60203599D1 (de) Methode zur Inaktivierung von TSE
ATE223047T1 (de) Immunchemische bestimmung von multivalenten analyten
ATE223491T1 (de) Verfahren zur reinigung von biomolekül- oder proteinkomplexen
DE60034966D1 (de) Auswertung von biologischen mitteln in lebenden zielzellen
CY1106349T1 (el) Συστηματα ανιχνευσης ανοσοπροσδιορισμου ιικου αντιγονου ηπατιτιδας c
EP1182458A4 (de) Verfahren zum nachweis von proteinen, die miteinander interagieren
DK1400589T3 (da) Fremgangsmåde til påvisning af prokaryotisk DNA
EP1308730A4 (de) Verfahren zum nachweis oder zum bestimmen von hbv
AU2002356520A1 (en) Detection of bovine viral diarrhea virus in tissue samples
DE69924617D1 (de) Immunologische Bestimmung des hydrophoben Lungensurfaktantproteins SP-C
ATE228655T1 (de) Trockene, analytische elemente zur bestimmung von proteinen
ATE278965T1 (de) Knorpelresorptionsassays

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties