ATE510028T1 - Verfahren zum elektrochemischen nachweis von zielnukleinsäuresequenzen - Google Patents

Verfahren zum elektrochemischen nachweis von zielnukleinsäuresequenzen

Info

Publication number
ATE510028T1
ATE510028T1 AT07731073T AT07731073T ATE510028T1 AT E510028 T1 ATE510028 T1 AT E510028T1 AT 07731073 T AT07731073 T AT 07731073T AT 07731073 T AT07731073 T AT 07731073T AT E510028 T1 ATE510028 T1 AT E510028T1
Authority
AT
Austria
Prior art keywords
nucleic acid
target sequence
acid sequences
electrochemical detection
target nucleic
Prior art date
Application number
AT07731073T
Other languages
English (en)
Inventor
Damien Marchal
Benoit Limoges
Murielle Dequaire
Original Assignee
Univ Paris Diderot Paris 7
Univ Bourgogne
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR0601936A external-priority patent/FR2898133B1/fr
Priority claimed from FR0700157A external-priority patent/FR2911149A1/fr
Application filed by Univ Paris Diderot Paris 7, Univ Bourgogne, Centre Nat Rech Scient filed Critical Univ Paris Diderot Paris 7
Application granted granted Critical
Publication of ATE510028T1 publication Critical patent/ATE510028T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/115—Characterised by chemical treatment oxidising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/305—Denaturation or renaturation by physical action
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT07731073T 2006-03-03 2007-03-02 Verfahren zum elektrochemischen nachweis von zielnukleinsäuresequenzen ATE510028T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0601936A FR2898133B1 (fr) 2006-03-03 2006-03-03 Methode de detection electrochimique de sequences cibles d'acide nucleique
FR0700157A FR2911149A1 (fr) 2007-01-10 2007-01-10 Methode de detection electrochimique de sequences cibles d'acide nucleique
PCT/FR2007/000373 WO2007099236A1 (fr) 2006-03-03 2007-03-02 Méthode de détection électrochimique de séquences cibles d'acide nucléique

Publications (1)

Publication Number Publication Date
ATE510028T1 true ATE510028T1 (de) 2011-06-15

Family

ID=38137572

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07731073T ATE510028T1 (de) 2006-03-03 2007-03-02 Verfahren zum elektrochemischen nachweis von zielnukleinsäuresequenzen

Country Status (6)

Country Link
US (1) US8313638B2 (de)
EP (1) EP1996725B1 (de)
JP (2) JP5320077B2 (de)
KR (1) KR101345077B1 (de)
AT (1) ATE510028T1 (de)
WO (1) WO2007099236A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4908182B2 (ja) * 2006-12-22 2012-04-04 栄研化学株式会社 核酸増幅の有無の判定方法、標的核酸の検出方法及びそれらに用いられる装置
WO2009023059A2 (en) 2007-06-01 2009-02-19 The Trustees Of Princeton University Treatment of viral infections by modulation of host cell metabolic pathways
FR2932191B1 (fr) * 2008-06-05 2010-12-03 Univ Paris Diderot Paris 7 Methode d'identification electrochimique de sequences cibles de nucleotides.
US8990059B2 (en) * 2009-10-21 2015-03-24 Koninklijke Philips N.V. Analyzing tool for amplification reactions
WO2013033647A2 (en) 2011-09-01 2013-03-07 The Regents Of The University Of California Apparatus and method for electrical detection of oligonucleotides through pore blockades
US9593371B2 (en) * 2013-12-27 2017-03-14 Intel Corporation Integrated photonic electronic sensor arrays for nucleic acid sequencing

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE223498T1 (de) * 1995-06-27 2002-09-15 Univ North Carolina Elektrochemische erkennung von nukleinsäurehybridation
US6361951B1 (en) 1995-06-27 2002-03-26 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
EP1090286A1 (de) * 1998-06-24 2001-04-11 Therasense, Inc. Mehrfacher sensorgruppe zur electrochemischer erkennung von nukleotidsequenzen und verfahren
JP3642574B2 (ja) * 2002-04-22 2005-04-27 北斗科学産業株式会社 遺伝子検出用具、検出方法及び検出用キット
US7892414B1 (en) * 2004-11-19 2011-02-22 The United States Of America As Represented By The Secretary Of Army Electrochemical biosensors, applications and methods of making biosensors

Also Published As

Publication number Publication date
JP2013153764A (ja) 2013-08-15
WO2007099236B1 (fr) 2007-12-13
KR101345077B1 (ko) 2013-12-26
JP2009528072A (ja) 2009-08-06
EP1996725B1 (de) 2011-05-18
KR20080111451A (ko) 2008-12-23
US8313638B2 (en) 2012-11-20
WO2007099236A1 (fr) 2007-09-07
HK1129909A1 (en) 2009-12-11
US20090065372A1 (en) 2009-03-12
EP1996725A1 (de) 2008-12-03
JP5320077B2 (ja) 2013-10-23

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