ATE552350T1 - Elektrokatalytischer nukleinsäurehybridisierungsnachweis - Google Patents

Elektrokatalytischer nukleinsäurehybridisierungsnachweis

Info

Publication number
ATE552350T1
ATE552350T1 AT05856910T AT05856910T ATE552350T1 AT E552350 T1 ATE552350 T1 AT E552350T1 AT 05856910 T AT05856910 T AT 05856910T AT 05856910 T AT05856910 T AT 05856910T AT E552350 T1 ATE552350 T1 AT E552350T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid hybridization
detection
hybridization detection
electrocatalytic
Prior art date
Application number
AT05856910T
Other languages
English (en)
Inventor
Shana Kelley
Rahela Gasparac
Melissa Lapierre-Devlin
Bradford Taft
Original Assignee
Trustees Boston College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trustees Boston College filed Critical Trustees Boston College
Application granted granted Critical
Publication of ATE552350T1 publication Critical patent/ATE552350T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6825Nucleic acid detection involving sensors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28Electrolytic cell components
    • G01N27/30Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT05856910T 2004-08-06 2005-08-05 Elektrokatalytischer nukleinsäurehybridisierungsnachweis ATE552350T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/913,925 US7741033B2 (en) 2003-05-13 2004-08-06 Electrocatalytic nucleic acid hybridization detection
PCT/US2005/027710 WO2006076047A2 (en) 2004-08-06 2005-08-05 Electrocatalytic nucleic acid hybridization detection

Publications (1)

Publication Number Publication Date
ATE552350T1 true ATE552350T1 (de) 2012-04-15

Family

ID=36678052

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05856910T ATE552350T1 (de) 2004-08-06 2005-08-05 Elektrokatalytischer nukleinsäurehybridisierungsnachweis

Country Status (6)

Country Link
US (1) US7741033B2 (de)
EP (1) EP1784512B1 (de)
AT (1) ATE552350T1 (de)
CA (1) CA2616259C (de)
ES (1) ES2385341T3 (de)
WO (1) WO2006076047A2 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1629122B1 (de) * 2003-05-13 2009-10-14 The Trustees of Boston College Elektrokatalytischer nukleinsäurehybridisierungsnachweis
JPWO2005103695A1 (ja) * 2004-04-23 2008-03-13 松下電器産業株式会社 遺伝子検出方法及び遺伝子検出装置
KR101076578B1 (ko) 2004-06-23 2011-10-24 더 보드 오브 리젠츠 오브 더 유니버시티 오브 텍사스 시스템 2입자 착물을 이용한 생물학적 분자의 검출을 위한 방법 및조성물
CA2604471A1 (en) * 2005-04-12 2007-08-23 The Trustees Of Boston College Method for electrocatalytic protein detection
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
WO2008127314A1 (en) 2006-11-22 2008-10-23 President And Fellows Of Harvard College High-sensitivity nanoscale wire sensors
ITMI20070393A1 (it) * 2007-02-28 2008-09-01 Nano Sims S R L Sensore elettrochimico, kit comprendente detto sensore e processo per la sua produzione
ES2310125B1 (es) * 2007-05-25 2010-01-07 Universidad Autonoma De Madrid Metodo para la deteccion electroquimica de secuencias de acidos nucleicos.
US9096430B2 (en) * 2007-06-13 2015-08-04 Board Of Regents, The University Of Texas System Method for metal nanoparticle electrocatalytic amplification
US20100105024A1 (en) * 2008-01-14 2010-04-29 Transgenex Nanobiotech, Inc. Rapid test including genetic sequence probe
WO2009128960A2 (en) * 2008-01-14 2009-10-22 Ultrapid Nanodiagnostics, Inc. Rapid test including genetic sequence probe
HRP20140460T1 (hr) 2008-04-11 2014-06-20 Board Of Regents Of The University Of Texas System Postupak i uređaj za pojačanje elektrogenerirane kemiluminescencije nanočestica
KR101603356B1 (ko) * 2008-05-08 2016-03-14 보드 오브 리전츠 오브 더 유니버시티 오브 텍사스 시스템 전기발생 화학발광에 사용되는 발광 나노구조 물질
JP5712129B2 (ja) 2008-09-02 2015-05-07 ザ ガバニング カウンシル オブ ザ ユニバーシティ オブ トロント ナノ構造化微小電極およびそれを組み込んだバイオセンシング装置
US9297796B2 (en) 2009-09-24 2016-03-29 President And Fellows Of Harvard College Bent nanowires and related probing of species
US8808530B2 (en) 2010-08-19 2014-08-19 Board Of Regents, The University Of Texas System Method and apparatus for electrocatalytic amplification on pre-oxidized measuring electrode
ES2704685T3 (es) 2011-01-11 2019-03-19 Governing Council Univ Toronto Método para la detección de proteínas
ES2818586T3 (es) * 2011-02-07 2021-04-13 Governing Council Univ Toronto Biosondas y métodos de uso de las mismas
US9580742B2 (en) 2011-03-10 2017-02-28 Shana O. Kelley Diagnostic and sample preparation devices and methods
RU2509157C2 (ru) * 2011-10-26 2014-03-10 Закрытое акционерное общество "КАРСИ" (ЗАО "КАРСИ") Способ детекции специфических последовательностей нуклеиновых кислот (варианты) и устройство для его осуществления
WO2013176773A1 (en) 2012-05-24 2013-11-28 The Governing Council Of The University Of Toronto Systems and methods for multiplexed electrochemical detection
CA2884531A1 (en) * 2012-09-12 2014-03-20 Xagenic Inc. Systems, devices, and methods for identifying a disease state in a biological host using internal controls
WO2014055888A1 (en) 2012-10-04 2014-04-10 The Regents Of The University Of California Rna-based, amplification-free, organism identification using nano-enabled electronic detection
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
WO2017155858A1 (en) * 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
WO2020122922A1 (en) * 2018-12-13 2020-06-18 Hewlett-Packard Development Company, L.P. Multiplex nucleic acid detection
EP3937780A4 (de) 2019-03-14 2022-12-07 InSilixa, Inc. Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion
CN113030032B (zh) * 2019-12-24 2022-06-24 Tcl科技集团股份有限公司 一种四环素的检测方法
US20240044833A1 (en) * 2020-09-14 2024-02-08 Mcmaster University Electrochemical biosensor for target analyte detection
CN113406159A (zh) * 2021-06-17 2021-09-17 海南大学 一种检测赭曲霉毒素a的电化学发光免疫传感器的制备和应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776672A (en) * 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
US5288390A (en) 1992-03-30 1994-02-22 Sun Company, Inc. (R&M) Polycyclic aromatic ring cleavage (PARC) process
US5312527A (en) * 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
CA2198489A1 (en) * 1994-08-26 1996-03-07 Igen International, Inc. Biosensor for and method of electrogenerated chemiluminescent detection of nucleic acid adsorbed to a solid surface
US6180346B1 (en) * 1995-06-27 2001-01-30 The Universtiy Of North Carolina At Chapel Hill Electropolymerizable film, and method of making and use thereof
US5968745A (en) * 1995-06-27 1999-10-19 The University Of North Carolina At Chapel Hill Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof
US6361951B1 (en) * 1995-06-27 2002-03-26 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
JP2000516708A (ja) * 1996-08-08 2000-12-12 ウィリアム・マーシュ・ライス・ユニバーシティ ナノチューブ組立体から作製された巨視的操作可能なナノ規模の装置
US6096273A (en) * 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US6221586B1 (en) * 1997-04-09 2001-04-24 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
US6872527B2 (en) * 1997-04-16 2005-03-29 Xtrana, Inc. Nucleic acid archiving
US6123819A (en) * 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
WO1999067628A1 (en) * 1998-06-24 1999-12-29 Therasense, Inc. Multi-sensor array for electrochemical recognition of nucleotide sequences and methods
US20030087277A1 (en) * 1998-12-23 2003-05-08 Wolfgang Fritzsche Means and methods for detection of binding of members of specific binding pairs
CZ20012503A3 (cs) 1999-01-18 2002-01-16 Friz Biochem Gmbh Metoda elektrochemické detekce hybridizačních procesů oligomeru nukleové kyseliny
WO2000060125A2 (en) * 1999-04-07 2000-10-12 Dennis Michael Connolly High resolution dna detection methods and devices
DE60035123T2 (de) * 1999-11-15 2008-01-31 Therasense, Inc., Alameda Übergangsmetallkomplexe, die über ein bewegliches zwischenglied an ein polymer gebunden sind
US7301199B2 (en) * 2000-08-22 2007-11-27 President And Fellows Of Harvard College Nanoscale wires and related devices
US6958216B2 (en) 2001-01-10 2005-10-25 The Trustees Of Boston College DNA-bridged carbon nanotube arrays
US7189435B2 (en) * 2001-03-14 2007-03-13 University Of Massachusetts Nanofabrication
EP2801624B1 (de) 2001-03-16 2019-03-06 Singular Bio, Inc Arrays und Verfahren zur Verwendung
AU2002367748A1 (en) * 2001-08-08 2003-10-27 North Carolina State University Infectious disease microarray
US20040072263A1 (en) * 2002-04-19 2004-04-15 Baylor College Of Medicine Quantitative measurement of proteins using genetically-engineered glucose oxidase fusion molecules
US7022287B2 (en) * 2002-05-08 2006-04-04 Sandia National Laboratories Single particle electrochemical sensors and methods of utilization
DE60230126D1 (de) * 2002-06-20 2009-01-15 St Microelectronics Srl Molekularspeicher mit DNA Strängen als molekulare Schalter und Kohlenstoffnanoröhren sowie dazugehöriges Herstellungsverfahren
US20040136866A1 (en) * 2002-06-27 2004-07-15 Nanosys, Inc. Planar nanowire based sensor elements, devices, systems and methods for using and making same
AU2003267583A1 (en) 2002-09-19 2004-04-08 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US7163659B2 (en) * 2002-12-03 2007-01-16 Hewlett-Packard Development Company, L.P. Free-standing nanowire sensor and method for detecting an analyte in a fluid
EP1629122B1 (de) * 2003-05-13 2009-10-14 The Trustees of Boston College Elektrokatalytischer nukleinsäurehybridisierungsnachweis
CA2604471A1 (en) * 2005-04-12 2007-08-23 The Trustees Of Boston College Method for electrocatalytic protein detection

Also Published As

Publication number Publication date
ES2385341T3 (es) 2012-07-23
CA2616259A1 (en) 2006-07-20
EP1784512B1 (de) 2012-04-04
CA2616259C (en) 2013-06-11
WO2006076047A2 (en) 2006-07-20
EP1784512A4 (de) 2010-02-10
EP1784512A2 (de) 2007-05-16
US20050084881A1 (en) 2005-04-21
US7741033B2 (en) 2010-06-22
WO2006076047A3 (en) 2009-04-09

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