ATE516363T1 - Generischer kinase-/phosphatasetest mit einzelablesung - Google Patents
Generischer kinase-/phosphatasetest mit einzelablesungInfo
- Publication number
- ATE516363T1 ATE516363T1 AT08801776T AT08801776T ATE516363T1 AT E516363 T1 ATE516363 T1 AT E516363T1 AT 08801776 T AT08801776 T AT 08801776T AT 08801776 T AT08801776 T AT 08801776T AT E516363 T1 ATE516363 T1 AT E516363T1
- Authority
- AT
- Austria
- Prior art keywords
- kinase
- fluorophore
- readout
- molecule
- phosphatase
- Prior art date
Links
- 108091000080 Phosphotransferase Proteins 0.000 title abstract 5
- 102000020233 phosphotransferase Human genes 0.000 title abstract 5
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 title abstract 4
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 abstract 3
- 125000003118 aryl group Chemical group 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- KAKKHKRHCKCAGH-UHFFFAOYSA-L disodium;(4-nitrophenyl) phosphate;hexahydrate Chemical compound O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)C1=CC=C(OP([O-])([O-])=O)C=C1 KAKKHKRHCKCAGH-UHFFFAOYSA-L 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07115987 | 2007-09-10 | ||
| PCT/EP2008/007122 WO2009033580A1 (en) | 2007-09-10 | 2008-09-01 | Generic kinase/phosphatase assay with single readout |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE516363T1 true ATE516363T1 (de) | 2011-07-15 |
Family
ID=39865459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08801776T ATE516363T1 (de) | 2007-09-10 | 2008-09-01 | Generischer kinase-/phosphatasetest mit einzelablesung |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8309310B2 (de) |
| EP (1) | EP2201129B1 (de) |
| JP (1) | JP5108946B2 (de) |
| CN (1) | CN101802214A (de) |
| AT (1) | ATE516363T1 (de) |
| CA (1) | CA2698926C (de) |
| ES (1) | ES2368669T3 (de) |
| WO (1) | WO2009033580A1 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9650674B2 (en) * | 2012-10-19 | 2017-05-16 | Bioventures, Llc | Nucleic acid probes and methods of using the same |
| KR101847264B1 (ko) * | 2016-03-29 | 2018-04-10 | 국립암센터 | 항원 반응형 항체-형광염료 결합체 및 이를 이용한 표적 세포의 형광영상 검출방법 |
| US11636870B2 (en) | 2020-08-20 | 2023-04-25 | Denso International America, Inc. | Smoking cessation systems and methods |
| US11932080B2 (en) | 2020-08-20 | 2024-03-19 | Denso International America, Inc. | Diagnostic and recirculation control systems and methods |
| US11881093B2 (en) | 2020-08-20 | 2024-01-23 | Denso International America, Inc. | Systems and methods for identifying smoking in vehicles |
| US12017506B2 (en) | 2020-08-20 | 2024-06-25 | Denso International America, Inc. | Passenger cabin air control systems and methods |
| US12377711B2 (en) | 2020-08-20 | 2025-08-05 | Denso International America, Inc. | Vehicle feature control systems and methods based on smoking |
| US11828210B2 (en) | 2020-08-20 | 2023-11-28 | Denso International America, Inc. | Diagnostic systems and methods of vehicles using olfaction |
| US12269315B2 (en) | 2020-08-20 | 2025-04-08 | Denso International America, Inc. | Systems and methods for measuring and managing odor brought into rental vehicles |
| US11760169B2 (en) | 2020-08-20 | 2023-09-19 | Denso International America, Inc. | Particulate control systems and methods for olfaction sensors |
| US11760170B2 (en) | 2020-08-20 | 2023-09-19 | Denso International America, Inc. | Olfaction sensor preservation systems and methods |
| US11813926B2 (en) | 2020-08-20 | 2023-11-14 | Denso International America, Inc. | Binding agent and olfaction sensor |
| US12251991B2 (en) | 2020-08-20 | 2025-03-18 | Denso International America, Inc. | Humidity control for olfaction sensors |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040171034A1 (en) * | 2002-05-03 | 2004-09-02 | Brian Agnew | Compositions and methods for detection and isolation of phosphorylated molecules |
| US7432070B2 (en) | 2003-06-16 | 2008-10-07 | Pierce Biotechnology, Inc. | Homogenous assay for enzymatic activity |
| EP1674580A1 (de) * | 2004-12-23 | 2006-06-28 | F. Hoffmann-La Roche Ag | Verfahren zur Identifikation von Aktivatoren und/oder Inhibitoren von enzymatischen Aktivität |
| WO2006102129A2 (en) | 2005-03-18 | 2006-09-28 | Qtl Biosystems Llc | Metal mediated fluorescence superquenching assays for detection of phosphorylation and dephosphorylation of quencher-labeled substrates |
| WO2007016975A1 (en) | 2005-07-29 | 2007-02-15 | F. Hoffmann-La Roche Ag | Kinase and phosphatase assays based on fret |
| US7585643B2 (en) * | 2005-09-08 | 2009-09-08 | Marquette University | Method for detecting kinase activity with thiol reactive fluorescent reagents |
-
2008
- 2008-09-01 ES ES08801776T patent/ES2368669T3/es active Active
- 2008-09-01 CN CN200880106373A patent/CN101802214A/zh active Pending
- 2008-09-01 AT AT08801776T patent/ATE516363T1/de not_active IP Right Cessation
- 2008-09-01 JP JP2010523310A patent/JP5108946B2/ja not_active Expired - Fee Related
- 2008-09-01 CA CA2698926A patent/CA2698926C/en active Active
- 2008-09-01 EP EP08801776A patent/EP2201129B1/de active Active
- 2008-09-01 WO PCT/EP2008/007122 patent/WO2009033580A1/en not_active Ceased
- 2008-09-01 US US12/677,127 patent/US8309310B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2698926A1 (en) | 2009-03-19 |
| CN101802214A (zh) | 2010-08-11 |
| JP2010538606A (ja) | 2010-12-16 |
| CA2698926C (en) | 2012-08-14 |
| JP5108946B2 (ja) | 2012-12-26 |
| EP2201129B1 (de) | 2011-07-13 |
| WO2009033580A1 (en) | 2009-03-19 |
| US20100203554A1 (en) | 2010-08-12 |
| ES2368669T3 (es) | 2011-11-21 |
| EP2201129A1 (de) | 2010-06-30 |
| US8309310B2 (en) | 2012-11-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |