ATE517995T1 - Prozess zur chromosomalen integration und zum ersatz von dna-sequenzen in clostridia - Google Patents

Prozess zur chromosomalen integration und zum ersatz von dna-sequenzen in clostridia

Info

Publication number
ATE517995T1
ATE517995T1 AT07107017T AT07107017T ATE517995T1 AT E517995 T1 ATE517995 T1 AT E517995T1 AT 07107017 T AT07107017 T AT 07107017T AT 07107017 T AT07107017 T AT 07107017T AT E517995 T1 ATE517995 T1 AT E517995T1
Authority
AT
Austria
Prior art keywords
clostridia
replacement
dna sequences
chromosomal integration
chromosomal
Prior art date
Application number
AT07107017T
Other languages
English (en)
Inventor
Philippe Soucaille
Rainer Figge
Christian Croux
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37605677&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE517995(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Application granted granted Critical
Publication of ATE517995T1 publication Critical patent/ATE517995T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT07107017T 2006-10-03 2007-04-26 Prozess zur chromosomalen integration und zum ersatz von dna-sequenzen in clostridia ATE517995T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2006/066997 WO2008040387A1 (en) 2006-10-03 2006-10-03 Process for chromosomal integration and dna sequence replacement in clostridia

Publications (1)

Publication Number Publication Date
ATE517995T1 true ATE517995T1 (de) 2011-08-15

Family

ID=37605677

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07107017T ATE517995T1 (de) 2006-10-03 2007-04-26 Prozess zur chromosomalen integration und zum ersatz von dna-sequenzen in clostridia

Country Status (18)

Country Link
US (2) US8168434B2 (de)
EP (2) EP2316952A1 (de)
JP (1) JP5203375B2 (de)
KR (1) KR101311619B1 (de)
CN (1) CN101528930B (de)
AR (1) AR063126A1 (de)
AT (1) ATE517995T1 (de)
AU (1) AU2006349179A1 (de)
BR (1) BRPI0622182B1 (de)
CA (1) CA2665120A1 (de)
CL (1) CL2007002857A1 (de)
DK (1) DK1908840T3 (de)
ES (1) ES2369859T3 (de)
IL (1) IL197844A0 (de)
MX (1) MX2009003605A (de)
NO (1) NO20091371L (de)
SI (1) SI1908840T1 (de)
WO (1) WO2008040387A1 (de)

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US7432090B2 (en) * 2004-07-01 2008-10-07 Rice University Blocking sporulation by inhibiting SpoIIE
EP2084288A1 (de) 2006-10-31 2009-08-05 Metabolic Explorer Verfahren zur biologischen herstellung von 1,3-propandiol aus glycerin mit hoher ausbeute
EP2192177A1 (de) 2008-11-28 2010-06-02 Total S.A. Cellulase-Cel5H-assoziierte Reagenzien und deren Verwendung in Mikroorganismen
WO2010022763A1 (en) * 2008-08-25 2010-03-04 Metabolic Explorer Method for the preparation of 2-hydroxy-isobutyrate
EP2194120A1 (de) 2008-12-02 2010-06-09 Total S.A. Bio-Verarbeitung von Lignocellulose zu Ethanol mit rekombinantem Clostridium
BRPI0923748B1 (pt) 2008-12-31 2019-04-09 Metabolic Explorer Método para a preparação de dióis
GB0901001D0 (en) * 2009-01-22 2009-03-04 Univ Nottingham Methods
EP2267126A1 (de) * 2009-06-26 2010-12-29 Metabolic Explorer Verfahren zur stabilen Genunterbrechung in Clostridia
EP2267141A1 (de) * 2009-06-26 2010-12-29 Metabolic Explorer Verfahren zur biologischen Herstellung von n-Butanol mit hohem Ertrag
KR101647143B1 (ko) * 2009-09-22 2016-08-10 지에스칼텍스 주식회사 클로스트리듐 속 미생물 유전자를 불활성화시키는 방법
US20110117655A1 (en) 2009-11-18 2011-05-19 Bryan Tracy Methods and compositions for genetically manipulating clostridia and related bacteria with homologous recombination associated proteins
EP2588598B1 (de) 2010-07-02 2016-03-09 Metabolic Explorer Verfahren zur herstellung von hydroxysäuren
TWI500768B (zh) 2010-07-05 2015-09-21 Metabolic Explorer Sa 由蔗糖製備１，３－丙二醇之方法
WO2012045088A2 (en) 2010-10-01 2012-04-05 The Board Of Trustees Of The University Of Illinois Combinatorial design of highly efficient heterologous pathways
AR083799A1 (es) 2010-11-10 2013-03-20 Metabolic Explorer Sa Microorganismos para la produccion de 1,3-propanodiol usando concentracion de glicerina alta
EP2643456B1 (de) 2010-11-24 2016-05-11 Novartis International Pharmaceutical Ltd. Fusionsproteine mit n-acetylglucosaminyltransferase aktivitàt
CN102559704B (zh) * 2010-12-23 2014-08-27 中国科学院上海生命科学研究院 一种在丙酮丁醇梭菌中敲除基因的方法
BR112013020067A2 (pt) 2011-01-21 2019-09-24 Univ Illinois fermentação aperfeiçoada de celodextrina e b-d-glicose
CN103597084A (zh) 2011-02-07 2014-02-19 加利福尼亚大学董事会 增强的纤维糊精代谢
US8692024B2 (en) 2011-02-14 2014-04-08 Eastman Renewable Materials, Llc Method of producing n-butyraldehyde
US9267106B2 (en) 2011-02-14 2016-02-23 Eastman Renewable Materials, Llc Method for incorporation of recombinant DNA
EP2540834A1 (de) 2011-06-29 2013-01-02 Metabolic Explorer Verfahren zur Herstellung von 1,3-Propandiol
EP2766492B1 (de) 2011-10-11 2018-06-06 Metabolic Explorer Fermentative Herstellung von Prenol
WO2013059326A1 (en) 2011-10-17 2013-04-25 The Board Of Trustees Of The University Of Illinois Xylitol production from cellulosic biomass
FR2982276A1 (fr) 2011-11-08 2013-05-10 Univ Grenoble 1 Cassette d'expression pour modifier le genome des bacteries et pour modifier un vecteur dans une souche bacterienne
CN103125447B (zh) * 2011-11-24 2014-10-15 上海西普尔-必凯实验动物有限公司 一种1号染色体替换实验小鼠品系C57BL/6-Chr1C3H/HeJ的构建
WO2013133882A2 (en) * 2011-12-16 2013-09-12 University Of Delaware Recombinant clostridium organism and method for isolation of double-crossover allelic exchange mutants
EP2820134A1 (de) 2012-03-02 2015-01-07 Metabolic Explorer Verfahren zur butanolherstellung
EP2647718A3 (de) 2012-04-06 2014-12-24 Metabolic Explorer Verfahren zur Herstellung von 5-Aminopentanoat unter Verwendung eines rekombinanten Mikroorganismus
WO2013155481A1 (en) 2012-04-13 2013-10-17 The Board Of Trustees Of The University Of Illinois Improved cellodextrin transport and mixed sugar fermentation
WO2014049382A2 (en) 2012-09-26 2014-04-03 Metabolic Explorer Ethylenediamine fermentative production by a recombinant microorganism
CN103719021B (zh) * 2012-10-10 2017-06-20 上海西普尔-必凯实验动物有限公司 1号染色体替换野生小家鼠品系C57BL/6.枣庄2‑Chr1的构建
CA2921430C (en) * 2013-09-12 2018-04-24 Lanzatech New Zealand Limited Recombinant microorganisms and methods of use thereof
GB201406970D0 (en) * 2014-04-17 2014-06-04 Green Biologics Ltd Targeted mutations
GB201406968D0 (en) * 2014-04-17 2014-06-04 Green Biologics Ltd Deletion mutants
PT3328997T (pt) 2015-07-27 2021-03-15 Centre Nat Rech Scient Novo polipéptido com actividade de ferredoxina-nadp+ redutase, polinucleótido codificador do mesmo e suas utilizações
US10801050B2 (en) 2015-10-23 2020-10-13 Metabolic Explorer Microorganism modified for the assimilation of levulinic acid
DK3583221T3 (da) 2017-02-20 2021-09-06 Metabolic Explorer Sa Mikrobielt konsortium omfattende clostridia til fremstilling af 1,3-propandiol ud fra glycerol
EP3438270A1 (de) 2017-08-04 2019-02-06 Metabolic Explorer Mikroorganismus und verfahren zur verbesserten 1,3-propandiol-herstellung durch fermentierung auf einem kulturmedium mit hohem glyceringehalt
BR112021002573A2 (pt) 2018-08-10 2021-05-11 Metabolic Explorer microrganismos com produção de 1,3-propanediol e ácido butírico melhorada
FR3111642B1 (fr) 2020-06-23 2024-11-29 Ifp Energies Now Souches de bacteriesclostridiumresistantes au 5-fluorouracile, outils genetiques et utilisations de ceux-ci
JP2022099419A (ja) * 2020-12-23 2022-07-05 トヨタ自動車株式会社 形質転換用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法
EP4587576A1 (de) 2022-09-15 2025-07-23 Institut National des Sciences Appliquées de Toulouse Genetisch modifizierter lignocellulolytischer clostridium acetobutylleucum

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US6673567B2 (en) * 2000-03-23 2004-01-06 E. I. Du Pont De Nemours And Company Method of determination of gene function
GB0008682D0 (en) * 2000-04-07 2000-05-31 Microbiological Res Authority Transformation of clostridium difficile
GB0414832D0 (en) * 2004-07-01 2004-08-04 Cobra Biolog Ltd Process for the removal of selectable marker gene sequences

Also Published As

Publication number Publication date
KR101311619B1 (ko) 2013-09-26
DK1908840T3 (da) 2011-09-19
US20120190116A1 (en) 2012-07-26
CA2665120A1 (en) 2008-04-10
BRPI0622182A2 (pt) 2011-12-27
NO20091371L (no) 2009-04-03
BRPI0622182B1 (pt) 2018-07-31
US8168434B2 (en) 2012-05-01
CL2007002857A1 (es) 2008-04-18
ES2369859T3 (es) 2011-12-07
KR20090086939A (ko) 2009-08-14
IL197844A0 (en) 2011-08-01
SI1908840T1 (sl) 2011-10-28
EP1908840A1 (de) 2008-04-09
US8628967B2 (en) 2014-01-14
US20080085558A1 (en) 2008-04-10
JP2010505398A (ja) 2010-02-25
MX2009003605A (es) 2009-04-22
AU2006349179A1 (en) 2008-04-10
EP1908840A8 (de) 2008-06-25
WO2008040387A1 (en) 2008-04-10
JP5203375B2 (ja) 2013-06-05
EP2316952A1 (de) 2011-05-04
CN101528930A (zh) 2009-09-09
EP1908840B1 (de) 2011-07-27
CN101528930B (zh) 2013-12-11
AR063126A1 (es) 2008-12-30

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