ATE518006T1 - Verfahren zur quantitativen analyse von methylcytosin in dna und verwendungen davon - Google Patents
Verfahren zur quantitativen analyse von methylcytosin in dna und verwendungen davonInfo
- Publication number
- ATE518006T1 ATE518006T1 AT06757697T AT06757697T ATE518006T1 AT E518006 T1 ATE518006 T1 AT E518006T1 AT 06757697 T AT06757697 T AT 06757697T AT 06757697 T AT06757697 T AT 06757697T AT E518006 T1 ATE518006 T1 AT E518006T1
- Authority
- AT
- Austria
- Prior art keywords
- methyl cytosine
- target gene
- quantitative analysis
- content
- dna
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 8
- HWPZZUQOWRWFDB-UHFFFAOYSA-N 1-methylcytosine Chemical compound CN1C=CC(N)=NC1=O HWPZZUQOWRWFDB-UHFFFAOYSA-N 0.000 title abstract 7
- 238000004445 quantitative analysis Methods 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 230000007067 DNA methylation Effects 0.000 abstract 2
- 206010028980 Neoplasm Diseases 0.000 abstract 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 abstract 2
- 201000011510 cancer Diseases 0.000 abstract 2
- 244000052769 pathogen Species 0.000 abstract 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 abstract 1
- 230000032683 aging Effects 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000005251 capillar electrophoresis Methods 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 1
- 230000002431 foraging effect Effects 0.000 abstract 1
- 150000004712 monophosphates Chemical class 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 230000001717 pathogenic effect Effects 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 229940035893 uracil Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/125—Bisulfite(s)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020050039210A KR100673811B1 (ko) | 2005-05-11 | 2005-05-11 | 유전자 내 메틸 시토신의 정량 분석방법 및 이의 사용용도 |
| PCT/KR2006/001765 WO2006121295A1 (en) | 2005-05-11 | 2006-05-11 | Method for quantitative analysis of methyl cytosine in dna and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE518006T1 true ATE518006T1 (de) | 2011-08-15 |
Family
ID=37396759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06757697T ATE518006T1 (de) | 2005-05-11 | 2006-05-11 | Verfahren zur quantitativen analyse von methylcytosin in dna und verwendungen davon |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1880023B1 (de) |
| KR (1) | KR100673811B1 (de) |
| AT (1) | ATE518006T1 (de) |
| WO (1) | WO2006121295A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2145180B1 (de) * | 2007-04-13 | 2013-12-04 | Sequenom, Inc. | Vergleichende sequenzanalyseverfahren und -systeme |
| KR101762295B1 (ko) * | 2012-02-10 | 2017-08-04 | (주)바이오니아 | 생체시료의 자동 분석 장치 및 방법 |
| US12180581B2 (en) | 2017-09-18 | 2024-12-31 | Waters Technologies Corporation | Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes |
| US12181452B2 (en) | 2017-09-18 | 2024-12-31 | Waters Technologies Corporation | Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes |
| US11918936B2 (en) | 2020-01-17 | 2024-03-05 | Waters Technologies Corporation | Performance and dynamic range for oligonucleotide bioanalysis through reduction of non specific binding |
| US12352734B2 (en) | 2020-09-24 | 2025-07-08 | Waters Technologies Corporation | Chromatographic hardware improvements for separation of reactive molecules |
| CN116200501A (zh) * | 2022-04-15 | 2023-06-02 | 广西民族大学 | 可口革囊星虫甲基化位点检测引物、扩增体系 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| US6960436B2 (en) | 2002-02-06 | 2005-11-01 | Epigenomics Ag | Quantitative methylation detection in DNA samples |
-
2005
- 2005-05-11 KR KR1020050039210A patent/KR100673811B1/ko not_active Expired - Fee Related
-
2006
- 2006-05-11 AT AT06757697T patent/ATE518006T1/de not_active IP Right Cessation
- 2006-05-11 EP EP06757697A patent/EP1880023B1/de not_active Not-in-force
- 2006-05-11 WO PCT/KR2006/001765 patent/WO2006121295A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1880023A1 (de) | 2008-01-23 |
| KR100673811B1 (ko) | 2007-01-24 |
| WO2006121295A1 (en) | 2006-11-16 |
| KR20060117437A (ko) | 2006-11-17 |
| EP1880023B1 (de) | 2011-07-27 |
| EP1880023A4 (de) | 2008-08-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |