ATE522622T1 - Vorrichtung und verfahren zur herstellung von nukleinsäuren für den nachweis - Google Patents

Vorrichtung und verfahren zur herstellung von nukleinsäuren für den nachweis

Info

Publication number
ATE522622T1
ATE522622T1 AT04810422T AT04810422T ATE522622T1 AT E522622 T1 ATE522622 T1 AT E522622T1 AT 04810422 T AT04810422 T AT 04810422T AT 04810422 T AT04810422 T AT 04810422T AT E522622 T1 ATE522622 T1 AT E522622T1
Authority
AT
Austria
Prior art keywords
sample
test
nucleic acid
manifold
target nucleic
Prior art date
Application number
AT04810422T
Other languages
English (en)
Inventor
Timothy Patno
Jennifer Hollenstein
Christian Kronshage
Christopher Khoury
Mark Weber
Tom Westberg
William Cork
Original Assignee
Nanosphere Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanosphere Inc filed Critical Nanosphere Inc
Application granted granted Critical
Publication of ATE522622T1 publication Critical patent/ATE522622T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00Supporting devices; Holding devices
    • B01L9/52Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/026Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877Flow chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT04810422T 2003-11-07 2004-11-04 Vorrichtung und verfahren zur herstellung von nukleinsäuren für den nachweis ATE522622T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/703,368 US7396677B2 (en) 2003-11-07 2003-11-07 Method of preparing nucleic acids for detection
PCT/US2004/036959 WO2005047855A2 (en) 2003-11-07 2004-11-04 Method of preparing nucleic acids for detection

Publications (1)

Publication Number Publication Date
ATE522622T1 true ATE522622T1 (de) 2011-09-15

Family

ID=34590724

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04810422T ATE522622T1 (de) 2003-11-07 2004-11-04 Vorrichtung und verfahren zur herstellung von nukleinsäuren für den nachweis

Country Status (7)

Country Link
US (2) US7396677B2 (de)
EP (1) EP1682681B1 (de)
JP (1) JP5753334B2 (de)
AT (1) ATE522622T1 (de)
AU (1) AU2004290369B2 (de)
CA (1) CA2544976A1 (de)
WO (1) WO2005047855A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US20030096321A1 (en) * 1999-05-19 2003-05-22 Jose Remacle Method for the identification and/or the quantification of a target compound obtained from a biological sample upon chips
CA2480990A1 (en) * 2002-04-11 2003-10-23 Sequenom, Inc. Methods and devices for performing chemical reactions on a solid support
US8298833B2 (en) * 2006-02-07 2012-10-30 Stokes Bio Limited Liquid bridge and system
JP5553602B2 (ja) * 2006-09-06 2014-07-16 キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッド マイクロ流体アッセイを実施するためのチップ及びカートリッジ設計構成
EP2143491A1 (de) * 2008-07-10 2010-01-13 Carpegen GmbH Vorrichtung zum Analysieren einer chemischen oder biologischen Probe
GB0815472D0 (en) * 2008-08-22 2008-10-01 Iti Scotland Ltd Microfluidic system
KR20110090394A (ko) * 2010-02-03 2011-08-10 삼성전자주식회사 마이크로어레이 반응 장치 및 이를 이용하는 방법
CN105733936B (zh) * 2014-12-12 2017-11-17 深圳华大基因研究院 基因测序仪
EP4115978A1 (de) * 2015-01-14 2023-01-11 Pixcell Medical Technologies Ltd. Wegwerfpatrone für probenflüssigkeitsanalyse
CN109564231B (zh) * 2016-06-08 2023-01-06 加利福尼亚大学董事会 用于处理组织和细胞的方法和装置
EP3820610A1 (de) 2018-07-12 2021-05-19 Luminex Corporation Systeme und verfahren zur durchführung variabler probenvorbereitung und analyseverfahren
CN113908899B (zh) * 2021-09-17 2023-05-02 圣湘生物科技股份有限公司 移液装置、核酸提取、检测装置及移液器更换方法
EP4732025A1 (de) * 2023-06-20 2026-04-29 Beckman Coulter, Inc. Küvettenreinigungsarbeitsablauf

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4978507A (en) * 1988-05-31 1990-12-18 Levin Andrew E Fluid flow manifold for blot type screening apparatus
US6645758B1 (en) * 1989-02-03 2003-11-11 Johnson & Johnson Clinical Diagnostics, Inc. Containment cuvette for PCR and method of use
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5512492A (en) * 1993-05-18 1996-04-30 University Of Utah Research Foundation Waveguide immunosensor with coating chemistry providing enhanced sensitivity
US6635226B1 (en) * 1994-10-19 2003-10-21 Agilent Technologies, Inc. Microanalytical device and use thereof for conducting chemical processes
US6524532B1 (en) * 1995-06-20 2003-02-25 The Regents Of The University Of California Microfabricated sleeve devices for chemical reactions
US20020022261A1 (en) * 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5716825A (en) * 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
US5811296A (en) * 1996-12-20 1998-09-22 Johnson & Johnson Clinical Diagnostics, Inc. Blocked compartments in a PCR reaction vessel
US6391622B1 (en) * 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US5985214A (en) * 1997-05-16 1999-11-16 Aurora Biosciences Corporation Systems and methods for rapidly identifying useful chemicals in liquid samples
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
JP3515381B2 (ja) 1998-09-10 2004-04-05 株式会社東芝 塩基配列検出装置および方法
US6942771B1 (en) * 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US6773676B2 (en) * 1999-04-27 2004-08-10 Agilent Technologies, Inc. Devices for performing array hybridization assays and methods of using the same
DE19928410C2 (de) * 1999-06-22 2002-11-28 Agilent Technologies Inc Gerätegehäuse mit einer Einrichtung zum Betrieb eines Labor-Mikrochips
DE19928412C2 (de) * 1999-06-22 2002-03-21 Agilent Technologies Inc Versorgungselement für einen Labor-Mikrochip
US6403379B1 (en) * 1999-09-03 2002-06-11 Array Biopharma Reactor plate washing station
AU2001234997B2 (en) 2000-02-11 2006-07-27 Monogram Biosciences, Inc. Microfluid device with sample injector and method of use
US6358387B1 (en) * 2000-03-27 2002-03-19 Caliper Technologies Corporation Ultra high throughput microfluidic analytical systems and methods
WO2002001081A2 (en) * 2000-06-23 2002-01-03 Micronics, Inc. Valve for use in microfluidic structures
FR2813207B1 (fr) 2000-08-28 2002-10-11 Bio Merieux Carte reactionnelle et utilisation d'une telle carte
GB2368809B (en) * 2000-09-15 2004-09-29 Norchip As Microfabricated reaction chamber system
JP3877572B2 (ja) * 2001-08-09 2007-02-07 オリンパス株式会社 微細流路装置およびその使用方法
AU2002363062B2 (en) * 2001-10-09 2007-03-22 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7186814B2 (en) * 2001-11-09 2007-03-06 Nanosphere, Inc. Bioconjugate-nanoparticle probes
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
JP3818926B2 (ja) * 2002-02-04 2006-09-06 富士写真フイルム株式会社 リセプター・リガンド会合反応方法
US7537936B2 (en) * 2002-05-31 2009-05-26 Agilent Technologies, Inc. Method of testing multiple fluid samples with multiple biopolymer arrays
US7205454B2 (en) * 2002-07-31 2007-04-17 Bayer Bioscience N.V. Corn root preferential promoters and uses thereof
US7390457B2 (en) * 2002-10-31 2008-06-24 Agilent Technologies, Inc. Integrated microfluidic array device
US7645574B2 (en) * 2003-01-23 2010-01-12 Integrated Nano-Technologies, Llc Methods of metallizing nucleic acid molecules and methods of attaching nucleic acid molecules to conductive surfaces
US7046357B2 (en) * 2003-01-30 2006-05-16 Ciphergen Biosystems, Inc. Apparatus for microfluidic processing and reading of biochip arrays

Also Published As

Publication number Publication date
JP2007510430A (ja) 2007-04-26
US20050112583A1 (en) 2005-05-26
WO2005047855A3 (en) 2006-04-06
US20080268458A1 (en) 2008-10-30
JP5753334B2 (ja) 2015-07-22
EP1682681A2 (de) 2006-07-26
EP1682681B1 (de) 2011-08-31
AU2004290369B2 (en) 2009-01-15
CA2544976A1 (en) 2005-05-26
US7396677B2 (en) 2008-07-08
EP1682681A4 (de) 2008-04-23
WO2005047855A2 (en) 2005-05-26
AU2004290369A1 (en) 2005-05-26

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