ATE522810T1 - Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnas - Google Patents
Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnasInfo
- Publication number
- ATE522810T1 ATE522810T1 AT08844765T AT08844765T ATE522810T1 AT E522810 T1 ATE522810 T1 AT E522810T1 AT 08844765 T AT08844765 T AT 08844765T AT 08844765 T AT08844765 T AT 08844765T AT E522810 T1 ATE522810 T1 AT E522810T1
- Authority
- AT
- Austria
- Prior art keywords
- methods
- compositions
- stranded
- detecting
- small target
- Prior art date
Links
- 108091032973 (ribonucleotides)n+m Proteins 0.000 title abstract 7
- 102000040650 (ribonucleotides)n+m Human genes 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 108700011259 MicroRNAs Proteins 0.000 abstract 1
- 239000011324 bead Substances 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 230000001575 pathological effect Effects 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US98350307P | 2007-10-29 | 2007-10-29 | |
| PCT/US2008/081520 WO2009058814A1 (en) | 2007-10-29 | 2008-10-29 | Methods and compositions for detection and enrichment of target small rnas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE522810T1 true ATE522810T1 (de) | 2011-09-15 |
Family
ID=40226874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08844765T ATE522810T1 (de) | 2007-10-29 | 2008-10-29 | Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnas |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US20100209933A1 (de) |
| EP (1) | EP2210098B1 (de) |
| AT (1) | ATE522810T1 (de) |
| WO (1) | WO2009058814A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101253261B1 (ko) * | 2011-05-11 | 2013-04-10 | 한국과학기술연구원 | ｓｉＲＮＡ 의 전달을 위한 재조합 단백질 및 이를 포함하는 조성물 |
| EP2388325A1 (de) * | 2010-05-20 | 2011-11-23 | RLP AgroScience GmbH | Verfahren zur Isolierung von Small-RNA-Molekülen unter Verwendung von HC-Pro-Protein |
| EP2428582B1 (de) * | 2010-09-14 | 2013-09-04 | miacom Diagnostics GmbH | Clearance-Puffer |
| WO2012125220A2 (en) | 2011-01-14 | 2012-09-20 | Life Technologies Corporation | Methods for isolation, identification, and quantification of mirnas |
| KR101430232B1 (ko) * | 2012-08-10 | 2014-08-18 | 한국과학기술연구원 | ｓｉＲＮＡ 전달을 위한 ｐ19-ＹＳＡ 재조합 단백질 및 이를 포함하는 조성물 |
| US9840703B2 (en) | 2013-01-11 | 2017-12-12 | Children's Medical Center Corporation | Methods and compositions for the production of siRNAs |
| WO2016178953A1 (en) * | 2015-05-01 | 2016-11-10 | The General Hospital Corporation | Multiplex analysis of gene expression in individual living cells |
| US11634769B2 (en) * | 2018-01-19 | 2023-04-25 | City University Of Hong Kong | Method of determining target nucleic acid |
| CN111607588B (zh) * | 2019-02-25 | 2024-06-25 | 上海印序生物科技有限公司 | 环状rna快速富集试剂盒及方法 |
| WO2021183921A1 (en) | 2020-03-12 | 2021-09-16 | New England Biolabs, Inc. | A rapid diagnostic test for lamp |
| EP4110945A2 (de) | 2020-08-21 | 2023-01-04 | New England Biolabs, Inc. | Schneller diagnosetest für lampe |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5643758A (en) | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| DE60212642T2 (de) * | 2001-04-10 | 2007-06-14 | ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE | Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen |
| US7517671B2 (en) * | 2002-02-28 | 2009-04-14 | New England Biolabs, Inc. | Methods and compositions for concentrating secreted recombinant protein |
| CN1717496A (zh) * | 2002-10-03 | 2006-01-04 | 洛桑生态综合技术联合公司 | 用o6－烷基鸟嘌呤－dna烷基转移酶标记蛋白 |
| CN1720332A (zh) * | 2002-10-03 | 2006-01-11 | 洛桑生态综合技术联合公司 | O6－烷基鸟嘌呤－dna烷基转移酶的底物 |
| JP2007509608A (ja) * | 2003-05-23 | 2007-04-19 | ウペエフエル・エコル・ポリテクニック・フェデラル・ドゥ・ローザンヌ | アシル担体タンパク質に基づくタンパク質をラベルするための方法 |
| WO2006032713A1 (es) * | 2004-01-13 | 2006-03-30 | Biomedal S.L. | USO DE PROTEÍNAS CON AFINIDAD POR ARNs CORTOS CON ACTIVIDAD SUPRESORA DEL SILENCIAMIENTO POSTTRANSCRIPCIONAL DE GENES |
| EP1730298B1 (de) * | 2004-03-02 | 2015-11-11 | EPFL Ecole Polytechnique Fédérale de Lausanne | Spezifische substrate für o6-alkylguanin-dna-alkyltransferase |
| EP1720981A2 (de) * | 2004-03-02 | 2006-11-15 | EPFL Ecole Polytechnique Fédérale de Lausanne | Mutanten der o6-alkylguanin-dna-alkyltransferase |
-
2008
- 2008-10-29 AT AT08844765T patent/ATE522810T1/de not_active IP Right Cessation
- 2008-10-29 US US12/738,580 patent/US20100209933A1/en not_active Abandoned
- 2008-10-29 WO PCT/US2008/081520 patent/WO2009058814A1/en not_active Ceased
- 2008-10-29 EP EP08844765A patent/EP2210098B1/de not_active Not-in-force
-
2012
- 2012-12-21 US US13/723,556 patent/US8753809B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20100209933A1 (en) | 2010-08-19 |
| EP2210098A1 (de) | 2010-07-28 |
| US20130109018A1 (en) | 2013-05-02 |
| WO2009058814A1 (en) | 2009-05-07 |
| US8753809B2 (en) | 2014-06-17 |
| EP2210098B1 (de) | 2011-08-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |