ATE522810T1 - Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnas - Google Patents
Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnasInfo
- Publication number
- ATE522810T1 ATE522810T1 AT08844765T AT08844765T ATE522810T1 AT E522810 T1 ATE522810 T1 AT E522810T1 AT 08844765 T AT08844765 T AT 08844765T AT 08844765 T AT08844765 T AT 08844765T AT E522810 T1 ATE522810 T1 AT E522810T1
- Authority
- AT
- Austria
- Prior art keywords
- methods
- compositions
- stranded
- detecting
- small target
- Prior art date
Links
- 108091032973 (ribonucleotides)n+m Proteins 0.000 title abstract 7
- 102000040650 (ribonucleotides)n+m Human genes 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 108700011259 MicroRNAs Proteins 0.000 abstract 1
- 239000011324 bead Substances 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 230000001575 pathological effect Effects 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US98350307P | 2007-10-29 | 2007-10-29 | |
| PCT/US2008/081520 WO2009058814A1 (en) | 2007-10-29 | 2008-10-29 | Methods and compositions for detection and enrichment of target small rnas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE522810T1 true ATE522810T1 (de) | 2011-09-15 |
Family
ID=40226874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08844765T ATE522810T1 (de) | 2007-10-29 | 2008-10-29 | Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnas |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US20100209933A1 (de) |
| EP (1) | EP2210098B1 (de) |
| AT (1) | ATE522810T1 (de) |
| WO (1) | WO2009058814A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101253261B1 (ko) * | 2011-05-11 | 2013-04-10 | 한국과학기술연구원 | ｓｉＲＮＡ 의 전달을 위한 재조합 단백질 및 이를 포함하는 조성물 |
| EP2388325A1 (de) * | 2010-05-20 | 2011-11-23 | RLP AgroScience GmbH | Verfahren zur Isolierung von Small-RNA-Molekülen unter Verwendung von HC-Pro-Protein |
| EP2428582B1 (de) * | 2010-09-14 | 2013-09-04 | miacom Diagnostics GmbH | Clearance-Puffer |
| US20120208189A1 (en) | 2011-01-14 | 2012-08-16 | Life Technologies Corporation | Methods for isolation, identification, and quantification of mirnas |
| KR101430232B1 (ko) * | 2012-08-10 | 2014-08-18 | 한국과학기술연구원 | ｓｉＲＮＡ 전달을 위한 ｐ19-ＹＳＡ 재조합 단백질 및 이를 포함하는 조성물 |
| US9840703B2 (en) | 2013-01-11 | 2017-12-12 | Children's Medical Center Corporation | Methods and compositions for the production of siRNAs |
| US10900070B2 (en) * | 2015-05-01 | 2021-01-26 | The General Hospital Corporation | Multiplex analysis of gene expression in individual living cells |
| US11634769B2 (en) * | 2018-01-19 | 2023-04-25 | City University Of Hong Kong | Method of determining target nucleic acid |
| CN111607588B (zh) * | 2019-02-25 | 2024-06-25 | 上海印序生物科技有限公司 | 环状rna快速富集试剂盒及方法 |
| EP4118233A1 (de) | 2020-03-12 | 2023-01-18 | New England Biolabs, Inc. | Schneller diagnosetest für lampe |
| EP4110945A2 (de) | 2020-08-21 | 2023-01-04 | New England Biolabs, Inc. | Schneller diagnosetest für lampe |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5643758A (en) | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| IL157880A0 (en) * | 2001-04-10 | 2004-03-28 | Ecole Polytech | Methods using o6-alkylguanine-dna alkyltransferases |
| US7517671B2 (en) * | 2002-02-28 | 2009-04-14 | New England Biolabs, Inc. | Methods and compositions for concentrating secreted recombinant protein |
| AU2003267423A1 (en) * | 2002-10-03 | 2004-04-23 | Ecole Polytechnique Federale De Lausanne (Epfl) | Protein labelling with 06-alkylguanine-DNA alkyltransferase |
| NZ538986A (en) * | 2002-10-03 | 2006-06-30 | Ecole Polytech | Substrates for O6-alkylguanine-DNA alkyltransferase |
| AU2004242249A1 (en) * | 2003-05-23 | 2004-12-02 | Epfl-Ecole Polytechnique Federale De Lausanne | Methods for protein labeling based on acyl carrier protein |
| WO2006032713A1 (es) * | 2004-01-13 | 2006-03-30 | Biomedal S.L. | USO DE PROTEÍNAS CON AFINIDAD POR ARNs CORTOS CON ACTIVIDAD SUPRESORA DEL SILENCIAMIENTO POSTTRANSCRIPCIONAL DE GENES |
| WO2005085431A2 (en) * | 2004-03-02 | 2005-09-15 | Epfl Ecole Polytechnique Federale De Lausanne | Mutants of o6-alkylguanine-dna alkyltransferase |
| WO2005085470A1 (en) * | 2004-03-02 | 2005-09-15 | Epfl Ecole Polytechnique Federale De Lausanne | Specific substrates for o6- alkylguanine-dna alkyltransferase |
-
2008
- 2008-10-29 WO PCT/US2008/081520 patent/WO2009058814A1/en not_active Ceased
- 2008-10-29 US US12/738,580 patent/US20100209933A1/en not_active Abandoned
- 2008-10-29 AT AT08844765T patent/ATE522810T1/de not_active IP Right Cessation
- 2008-10-29 EP EP08844765A patent/EP2210098B1/de not_active Not-in-force
-
2012
- 2012-12-21 US US13/723,556 patent/US8753809B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP2210098A1 (de) | 2010-07-28 |
| US20130109018A1 (en) | 2013-05-02 |
| WO2009058814A1 (en) | 2009-05-07 |
| EP2210098B1 (de) | 2011-08-31 |
| US8753809B2 (en) | 2014-06-17 |
| US20100209933A1 (en) | 2010-08-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |