ATE524559T1 - Methode zur verbesserung der proteinproduktion - Google Patents

Methode zur verbesserung der proteinproduktion

Info

Publication number
ATE524559T1
ATE524559T1 AT03779044T AT03779044T ATE524559T1 AT E524559 T1 ATE524559 T1 AT E524559T1 AT 03779044 T AT03779044 T AT 03779044T AT 03779044 T AT03779044 T AT 03779044T AT E524559 T1 ATE524559 T1 AT E524559T1
Authority
AT
Austria
Prior art keywords
protein
sequence
cell
trap
protein expression
Prior art date
Application number
AT03779044T
Other languages
English (en)
Inventor
Arie Otte
Blokland Henricus Van
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Application granted granted Critical
Publication of ATE524559T1 publication Critical patent/ATE524559T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT03779044T 2002-12-18 2003-12-02 Methode zur verbesserung der proteinproduktion ATE524559T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02080347 2002-12-18
PCT/NL2003/000850 WO2004055215A1 (en) 2002-12-18 2003-12-02 A method for improving protein production

Publications (1)

Publication Number Publication Date
ATE524559T1 true ATE524559T1 (de) 2011-09-15

Family

ID=32524041

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03779044T ATE524559T1 (de) 2002-12-18 2003-12-02 Methode zur verbesserung der proteinproduktion

Country Status (9)

Country Link
US (2) US7413896B2 (de)
EP (1) EP1573058B1 (de)
AT (1) ATE524559T1 (de)
AU (1) AU2003285823B2 (de)
CA (1) CA2510179C (de)
DK (1) DK1573058T3 (de)
ES (1) ES2373549T3 (de)
NZ (1) NZ540471A (de)
WO (1) WO2004055215A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1600510E (pt) 2001-07-04 2007-06-25 Chromagenics Bv Sequências de adn com actividade anti-repressora.
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
WO2003106674A2 (en) 2002-06-14 2003-12-24 Chromagenics B.V. Means and methods for regulating gene expression
ATE524559T1 (de) 2002-12-18 2011-09-15 Chromagenics Bv Methode zur verbesserung der proteinproduktion
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
ES2384391T3 (es) 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
KR101271884B1 (ko) * 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
US7732342B2 (en) * 2005-05-26 2010-06-08 Applied Materials, Inc. Method to increase the compressive stress of PECVD silicon nitride films
US7566655B2 (en) * 2005-05-26 2009-07-28 Applied Materials, Inc. Integration process for fabricating stressed transistor structure
MX2008000985A (es) 2005-07-21 2008-04-07 Abbott Lab Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis.
US20080293086A1 (en) * 2006-09-18 2008-11-27 Cobalt Technologies, Inc. A Delaware Corporation Real time monitoring of microbial enzymatic pathways
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
CN102057046A (zh) 2008-04-09 2011-05-11 钴技术有限公司 固定的产物耐受的微生物
US8497105B2 (en) 2009-06-26 2013-07-30 Cobalt Technologies, Inc. Integrated system and process for bioproduct production
WO2011053699A1 (en) * 2009-10-30 2011-05-05 Abbott Laboratories Sorf constructs and multiple gene expression
MY174677A (en) * 2013-08-21 2020-05-06 Curevac Ag Composition and vaccine for treating lung cancer

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5733782A (en) * 1993-10-25 1998-03-31 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
US5972605A (en) 1994-07-07 1999-10-26 Geron Corporation Assays for regulators of mammalian telomerase expression
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
AU771111B2 (en) 1998-07-21 2004-03-11 Emd Millipore Corporation A polynucleotide comprising a ubiquitous chromatin opening element (UCOE)
ES2292251T3 (es) * 1998-08-14 2008-03-01 Chromagenics B.V. Procedimiento de deteccion de una secuencia de adn, procedimiento de preparacion de una construccion de adn y uso de los mismos.
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6800457B2 (en) * 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2000023606A1 (en) 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
AU2001238178A1 (en) * 2000-02-11 2001-08-20 Millennium Pharmaceuticals, Inc. Seven-transmembrane proteins/g-protein coupled receptors
US6586205B1 (en) * 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
KR100996016B1 (ko) 2000-09-20 2010-11-22 밀리포어 코포레이션 폴리뉴클레오티드
US6787321B1 (en) * 2000-10-13 2004-09-07 The Regents Of The University Of California Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products
US7109029B2 (en) * 2001-02-23 2006-09-19 Cell Genesys, Inc. Vector constructs
PT1600510E (pt) * 2001-07-04 2007-06-25 Chromagenics Bv Sequências de adn com actividade anti-repressora.
EP1273666A1 (de) 2001-07-04 2003-01-08 Chromagenics B.V. Verfahren zur Selektion einer transkription-modulatierenden DNA-Sequenz unter Verwendung einem Vektor mit einem transkriptions-repressions-aktivem Element
ITMI20012110A1 (it) * 2001-10-12 2003-04-12 Keryos Spa Vettori multi-cistronici utilizzabili in protocolli di trsferimento genico
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
ATE524559T1 (de) * 2002-12-18 2011-09-15 Chromagenics Bv Methode zur verbesserung der proteinproduktion
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
KR101271884B1 (ko) 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
ES2384391T3 (es) 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof

Also Published As

Publication number Publication date
US7413896B2 (en) 2008-08-19
EP1573058A1 (de) 2005-09-14
US20060010506A1 (en) 2006-01-12
NZ540471A (en) 2008-07-31
DK1573058T3 (da) 2012-01-16
CA2510179A1 (en) 2004-07-01
AU2003285823A1 (en) 2004-07-09
ES2373549T3 (es) 2012-02-06
WO2004055215A1 (en) 2004-07-01
CA2510179C (en) 2013-04-23
EP1573058B1 (de) 2011-09-14
US20090011468A1 (en) 2009-01-08
AU2003285823B2 (en) 2008-06-19
US8173422B2 (en) 2012-05-08

Similar Documents

Publication Publication Date Title
ATE524559T1 (de) Methode zur verbesserung der proteinproduktion
DE602004020321D1 (de) Endomannosidasen zur modifzierung der glykoproteine in eukaryoten
FR2822164B1 (fr) Polypeptides derives des arn polymerases, et leurs utilisations
SG143066A1 (en) Dna sequences comprising gene transcription regulatory qualities and methods for detecting and using such dna sequences
DE60335195D1 (de) Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon
ATE374828T1 (de) Expressionsvektor zur antibiotikafreien expression
ATE469223T1 (de) Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
UA94019C2 (ru) Антитело, которое специфически связывается с глипиканом 3 (gpc3)
SG156672A1 (en) Methods for refolding of recombinant antibodies
ATE543506T1 (de) Methode zur stabilisierung von proteinen
ATE355382T1 (de) Verfahren zur fermentativen herstellung von l- threonine
TW200510450A (en) Soluble fragments of the SARS-CoV spike glycoprotein
ATE465241T1 (de) Verfahren zur fermentativen herstellung von proteinen
ATE516341T1 (de) Verfahren zur fermentativen herstellung von heterologen proteinen mittels escherichia coli
ATE492631T1 (de) Verwendung von mutationen zur verbesserung von aspergillus-phytasen
DE60219632D1 (de) Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon
DE602004027927D1 (de) Verwendung von labiatae-kräuterzubereitungen zur verbesserung des schaumes von getränken
ATE493503T1 (de) Verfahren zur fermentativen herstellung von proteinen
MX2023008774A (es) Composicion de vacuna para romper la autotolerancia.
ATE445009T1 (de) Signalpeptid zur herstellung eines polypeptids
ATE384790T1 (de) Uroplakin-ii-promotor aus schwein und verfahren zur herstellung nützlicher proteine unter verwendung des promotors
ATE451457T1 (de) Verfahren zur dipeptidherstellung
ATE510921T1 (de) Verfahren zur erzeugung cgmmv-resistenter pflanzen
WO2002033093A3 (en) Aggrecanase molecules
EP1181351A4 (de) Verfahren zur aufreinigung heterologer proteine

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1573058

Country of ref document: EP