ATE527338T1 - Verfahren zur gewinnung von nukleinsäuren durch lyse mikrobieller proben - Google Patents

Verfahren zur gewinnung von nukleinsäuren durch lyse mikrobieller proben

Info

Publication number
ATE527338T1
ATE527338T1 AT05804047T AT05804047T ATE527338T1 AT E527338 T1 ATE527338 T1 AT E527338T1 AT 05804047 T AT05804047 T AT 05804047T AT 05804047 T AT05804047 T AT 05804047T AT E527338 T1 ATE527338 T1 AT E527338T1
Authority
AT
Austria
Prior art keywords
nucleic acids
lysis
obtaining nucleic
microbial samples
methods
Prior art date
Application number
AT05804047T
Other languages
English (en)
Inventor
Yiu-Lian Fong
Azita Tabrizi
Original Assignee
Novartis Vaccines & Diagnostic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Vaccines & Diagnostic filed Critical Novartis Vaccines & Diagnostic
Application granted granted Critical
Publication of ATE527338T1 publication Critical patent/ATE527338T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT05804047T 2004-08-03 2005-08-03 Verfahren zur gewinnung von nukleinsäuren durch lyse mikrobieller proben ATE527338T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59863804P 2004-08-03 2004-08-03
PCT/US2005/027715 WO2006015374A2 (en) 2004-08-03 2005-08-03 Compositions and methods for preparation of nucleic acids from microbial samples

Publications (1)

Publication Number Publication Date
ATE527338T1 true ATE527338T1 (de) 2011-10-15

Family

ID=35787930

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05804047T ATE527338T1 (de) 2004-08-03 2005-08-03 Verfahren zur gewinnung von nukleinsäuren durch lyse mikrobieller proben

Country Status (5)

Country Link
US (1) US20080193912A1 (de)
EP (2) EP1774041B9 (de)
AT (1) ATE527338T1 (de)
ES (1) ES2371764T3 (de)
WO (1) WO2006015374A2 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2661168T3 (es) 2003-07-12 2018-03-27 Accelerate Diagnostics, Inc. Biodetección sensible y rápida
US20120077206A1 (en) 2003-07-12 2012-03-29 Accelr8 Technology Corporation Rapid Microbial Detection and Antimicrobial Susceptibility Testing
EP2255010B1 (de) 2008-02-20 2018-05-30 Streck Inc. Thermocycler und probengefäss zur schnellen amplifikation von dna
WO2009114520A2 (en) * 2008-03-10 2009-09-17 Pharmain Corporation Compositions for treatment with metallopeptidases, methods of making and using the same
WO2010132756A2 (en) * 2009-05-14 2010-11-18 Streck, Inc. Sample processing cassette, system, and method
WO2012050787A1 (en) * 2010-09-29 2012-04-19 Ibis Biosciences, Inc. Fungal nucleic acid extraction
US10254204B2 (en) 2011-03-07 2019-04-09 Accelerate Diagnostics, Inc. Membrane-assisted purification
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems
WO2012166913A1 (en) 2011-06-01 2012-12-06 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
EP2883039A1 (de) 2012-08-10 2015-06-17 Streck Inc. Optisches echtzeitsystem für echtzeit-polymerasekettenreaktion
CA2923795A1 (en) * 2012-09-10 2014-03-13 Accelerate Diagnostics, Inc. Same-day blood culture with digital microscopy
US9677109B2 (en) 2013-03-15 2017-06-13 Accelerate Diagnostics, Inc. Rapid determination of microbial growth and antimicrobial susceptibility
EP3014251A1 (de) 2013-06-28 2016-05-04 Streck Inc. Vorrichtungen für echtzeit-polymerasekettenreaktionen
US10023895B2 (en) 2015-03-30 2018-07-17 Accelerate Diagnostics, Inc. Instrument and system for rapid microogranism identification and antimicrobial agent susceptibility testing
US10253355B2 (en) 2015-03-30 2019-04-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
FR3035409B1 (fr) * 2015-04-21 2020-01-24 Fondation Mediterranee Infection Methode d'extraction d'adn comprenant un traitement de degradation enzymatique d'un echantillon biologique
US10888508B1 (en) * 2018-06-21 2021-01-12 Saied Mostafa Moazzami Multi-functional coolant for dentistry use
CN109402238A (zh) * 2018-12-19 2019-03-01 广州海思医疗科技有限公司 用于血液、口腔拭子进行pcr的裂解液及试剂盒
CN109486903A (zh) * 2018-12-25 2019-03-19 广州海思医疗科技有限公司 一种用于将拭子上的细胞洗脱、裂解释放核酸的洗脱裂解液、试剂盒和方法
CN110283816A (zh) * 2019-07-08 2019-09-27 无锡市第二人民医院 一种磁珠法微生物基因组dna的提取试剂盒及提取方法
CN110804611A (zh) * 2019-11-13 2020-02-18 北京贝尔生物工程股份有限公司 一种适用于细菌和/或真菌的基因组dna提取方法
CN112391384B (zh) * 2021-01-11 2023-03-10 甘肃省科学院传感技术研究所 一种快速提取革兰氏阴性菌基因组dna的试剂盒及提取方法
CN116334071A (zh) * 2022-08-26 2023-06-27 广州盘古医学检验实验室有限公司 一种用于检测病原微生物的核酸提取试剂盒及其提取方法
EP4389911A1 (de) 2022-12-21 2024-06-26 Mobidiag Oy Verfahren und systeme zur isolierung eines analyten
CN116694472A (zh) * 2023-07-18 2023-09-05 墨卓生物科技(浙江)有限公司 一种微生物裂解液、裂解方法及试剂盒

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5700636A (en) * 1990-10-19 1997-12-23 Becton Dickinson And Company Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples
DE4236708A1 (de) * 1992-10-30 1994-05-05 Bayer Ag Spezifische Gensonden und Verfahren zur Diagnostik von Candida albicans
DE4321904B4 (de) 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
ZA99493B (en) 1998-01-30 1999-07-22 Akzo Nobel Nv Method for the isolation of nucleic acid.
US6265164B1 (en) * 1998-03-26 2001-07-24 Biochain Institute, Inc. Compositions and methods for directly and rapidly analyzing the biochemical components of microorganisms
JP3818416B2 (ja) 1999-04-14 2006-09-06 東洋紡績株式会社 粒子担体を使用した核酸の抽出方法
GB9928676D0 (en) * 1999-12-03 2000-02-02 Provalis Uk Ltd Pseudomonas aeruginosa antigens
DE60210621T2 (de) * 2001-07-19 2007-03-08 Infectio Diagnostic (I.D.I.) Inc., Sainte-Foy Universelle methode und zusammensetzung zur schnellen lysierung von zellen zur freisetzung von nukleinsäuren und ihre detektion
US7374888B2 (en) * 2002-01-08 2008-05-20 Biosite Incorporated Methods for detecting B. anthracis infection
EP1527172B1 (de) * 2002-05-17 2008-11-26 Molzym GmbH & Co. KG Methode für die extraktion und aufreinigung von nukleinsäuren

Also Published As

Publication number Publication date
EP1774041B9 (de) 2013-04-17
EP1774041A4 (de) 2009-01-21
WO2006015374A2 (en) 2006-02-09
US20080193912A1 (en) 2008-08-14
EP1774041B1 (de) 2011-10-05
ES2371764T3 (es) 2012-01-09
EP2361993A1 (de) 2011-08-31
WO2006015374A3 (en) 2007-11-08
EP1774041A2 (de) 2007-04-18

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