ATE527366T1 - Verfahren zur mischung von vielen heterologen genen - Google Patents

Verfahren zur mischung von vielen heterologen genen

Info

Publication number
ATE527366T1
ATE527366T1 AT03787738T AT03787738T ATE527366T1 AT E527366 T1 ATE527366 T1 AT E527366T1 AT 03787738 T AT03787738 T AT 03787738T AT 03787738 T AT03787738 T AT 03787738T AT E527366 T1 ATE527366 T1 AT E527366T1
Authority
AT
Austria
Prior art keywords
methods
cells
heterologous genes
genes
evolution
Prior art date
Application number
AT03787738T
Other languages
English (en)
Inventor
Alexandra Sorensen
Soren Nielsen
Neil Goldsmith
Curt Nielsen
Original Assignee
Evolva Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Ltd filed Critical Evolva Ltd
Application granted granted Critical
Publication of ATE527366T1 publication Critical patent/ATE527366T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Processing And Handling Of Plastics And Other Materials For Molding In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT03787738T 2002-08-01 2003-08-01 Verfahren zur mischung von vielen heterologen genen ATE527366T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200201173 2002-08-01
DKPA200300787 2003-05-23
PCT/DK2003/000521 WO2004016791A1 (en) 2002-08-01 2003-08-01 Methods of mixing large numbers of heterologous genes

Publications (1)

Publication Number Publication Date
ATE527366T1 true ATE527366T1 (de) 2011-10-15

Family

ID=31889347

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03787738T ATE527366T1 (de) 2002-08-01 2003-08-01 Verfahren zur mischung von vielen heterologen genen

Country Status (6)

Country Link
US (1) US20060252156A1 (de)
EP (1) EP1529110B1 (de)
AT (1) ATE527366T1 (de)
AU (1) AU2003247270A1 (de)
DK (1) DK1529110T3 (de)
WO (1) WO2004016791A1 (de)

Families Citing this family (12)

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WO2002059297A2 (en) * 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
US20050164162A1 (en) * 2002-01-25 2005-07-28 Evolva Ltd., C/O Dr. Iur. Martin Eisenring Methods for multiple parameters screening and evolution of cells to produce small molecules with multiple functionalities
CA2585098C (en) 2004-10-22 2018-12-18 Revivicor, Inc. Porcine genomic kappa and lambda light chain sequences
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
AU2007332980A1 (en) * 2006-07-26 2008-06-19 Intrexon Corporation Methods and compositions for treating disease
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
CN101343630B (zh) * 2007-07-12 2012-11-07 中国科学院上海生命科学研究院 利用基因组改组技术改良多拉菌素生产菌的方法
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
CA2806148A1 (en) 2010-07-30 2012-02-02 Glycode A yeast artificial chromosome carrying the mammalian glycosylation pathway
CN107287185B (zh) * 2017-07-22 2021-05-18 贵州省园艺研究所 马铃薯四倍体栽培种与二倍体野生种原生质体融合方法

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US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
DK1364005T3 (da) * 2001-01-25 2009-02-02 Evolva Ltd En metode til udvikling af en celle der har en önsket fænotype og udviklede celler
WO2002059297A2 (en) * 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
CA2474161C (en) * 2001-01-25 2012-03-27 Evolva Ltd. Concatemers of differentially expressed multiple genes
US20050164162A1 (en) * 2002-01-25 2005-07-28 Evolva Ltd., C/O Dr. Iur. Martin Eisenring Methods for multiple parameters screening and evolution of cells to produce small molecules with multiple functionalities
WO2003106639A2 (en) * 2002-06-14 2003-12-24 Dyax Corporation Recombination of nucleic acid library members
US20030235823A1 (en) * 2002-06-24 2003-12-25 The University Of Alabama Nucleotide sequences that code for torsin genes, torsin proteins, and methods of using the same to treat protein-aggregation

Also Published As

Publication number Publication date
EP1529110B1 (de) 2011-10-05
EP1529110A1 (de) 2005-05-11
WO2004016791A1 (en) 2004-02-26
US20060252156A1 (en) 2006-11-09
AU2003247270A1 (en) 2004-03-03
DK1529110T3 (da) 2012-01-30

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