ATE527366T1 - Verfahren zur mischung von vielen heterologen genen - Google Patents

Verfahren zur mischung von vielen heterologen genen

Info

Publication number
ATE527366T1
ATE527366T1 AT03787738T AT03787738T ATE527366T1 AT E527366 T1 ATE527366 T1 AT E527366T1 AT 03787738 T AT03787738 T AT 03787738T AT 03787738 T AT03787738 T AT 03787738T AT E527366 T1 ATE527366 T1 AT E527366T1
Authority
AT
Austria
Prior art keywords
methods
cells
heterologous genes
genes
evolution
Prior art date
Application number
AT03787738T
Other languages
English (en)
Inventor
Alexandra Sorensen
Soren Nielsen
Neil Goldsmith
Curt Nielsen
Original Assignee
Evolva Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Ltd filed Critical Evolva Ltd
Application granted granted Critical
Publication of ATE527366T1 publication Critical patent/ATE527366T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing And Handling Of Plastics And Other Materials For Molding In General (AREA)
AT03787738T 2002-08-01 2003-08-01 Verfahren zur mischung von vielen heterologen genen ATE527366T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200201173 2002-08-01
DKPA200300787 2003-05-23
PCT/DK2003/000521 WO2004016791A1 (en) 2002-08-01 2003-08-01 Methods of mixing large numbers of heterologous genes

Publications (1)

Publication Number Publication Date
ATE527366T1 true ATE527366T1 (de) 2011-10-15

Family

ID=31889347

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03787738T ATE527366T1 (de) 2002-08-01 2003-08-01 Verfahren zur mischung von vielen heterologen genen

Country Status (6)

Country Link
US (1) US20060252156A1 (de)
EP (1) EP1529110B1 (de)
AT (1) ATE527366T1 (de)
AU (1) AU2003247270A1 (de)
DK (1) DK1529110T3 (de)
WO (1) WO2004016791A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059297A2 (en) * 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1472349B1 (de) * 2002-01-25 2008-10-29 Evolva AG Screeningmethoden nach mehreren parametern und entwicklung von zellen, um kleine moleküle mit mehreren funktionalitäten zu produzieren
CA2958259C (en) 2004-10-22 2020-06-30 Revivicor, Inc. Ungulates with genetically modified immune systems
CN103589650A (zh) 2005-03-18 2014-02-19 米克罗比亚公司 产油酵母和真菌中类胡萝卜素的产生
EP2043662B1 (de) * 2006-07-26 2015-09-09 Intrexon Corporation Verfahren und zusammensetzungen zur behandlung von krankheiten
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
CN101343630B (zh) * 2007-07-12 2012-11-07 中国科学院上海生命科学研究院 利用基因组改组技术改良多拉菌素生产菌的方法
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
JP2013535198A (ja) 2010-07-30 2013-09-12 グリコド 哺乳類のグリコシル化経路を有する酵母人工染色体
CN107287185B (zh) * 2017-07-22 2021-05-18 贵州省园艺研究所 马铃薯四倍体栽培种与二倍体野生种原生质体融合方法

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WO2002059297A2 (en) * 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
WO2002059290A2 (en) * 2001-01-25 2002-08-01 Evolva Biotech A/S A method for evolving a cell having desired phenotype and envolved cells
CA2474161C (en) * 2001-01-25 2012-03-27 Evolva Ltd. Concatemers of differentially expressed multiple genes
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1472349B1 (de) * 2002-01-25 2008-10-29 Evolva AG Screeningmethoden nach mehreren parametern und entwicklung von zellen, um kleine moleküle mit mehreren funktionalitäten zu produzieren
DE60319259T2 (de) * 2002-06-14 2009-03-05 Dyax Corp., Cambridge Rekombination von nukleinsäurebibliotheksmitgliedern
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Also Published As

Publication number Publication date
US20060252156A1 (en) 2006-11-09
AU2003247270A1 (en) 2004-03-03
EP1529110B1 (de) 2011-10-05
WO2004016791A1 (en) 2004-02-26
EP1529110A1 (de) 2005-05-11
DK1529110T3 (da) 2012-01-30

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