ATE530636T1 - Zellkultur - Google Patents

Zellkultur

Info

Publication number
ATE530636T1
ATE530636T1 AT07712662T AT07712662T ATE530636T1 AT E530636 T1 ATE530636 T1 AT E530636T1 AT 07712662 T AT07712662 T AT 07712662T AT 07712662 T AT07712662 T AT 07712662T AT E530636 T1 ATE530636 T1 AT E530636T1
Authority
AT
Austria
Prior art keywords
cell
inhibitor
differentiation
cadherin activity
cell culture
Prior art date
Application number
AT07712662T
Other languages
English (en)
Inventor
Christopher Ward
Original Assignee
Univ Manchester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Manchester filed Critical Univ Manchester
Application granted granted Critical
Publication of ATE530636T1 publication Critical patent/ATE530636T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/58—Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Reproductive Health (AREA)
  • Biochemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT07712662T 2006-02-02 2007-02-02 Zellkultur ATE530636T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0602063.0A GB0602063D0 (en) 2006-02-02 2006-02-02 Cell Culture
PCT/GB2007/000356 WO2007088372A2 (en) 2006-02-02 2007-02-02 Cell culture

Publications (1)

Publication Number Publication Date
ATE530636T1 true ATE530636T1 (de) 2011-11-15

Family

ID=36100877

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07712662T ATE530636T1 (de) 2006-02-02 2007-02-02 Zellkultur

Country Status (5)

Country Link
US (1) US8883501B2 (de)
EP (1) EP1979470B1 (de)
AT (1) ATE530636T1 (de)
GB (1) GB0602063D0 (de)
WO (1) WO2007088372A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US8586357B2 (en) 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
CN103898047B (zh) 2003-12-23 2020-03-03 维亚希特公司 定形内胚层
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
WO2007051038A2 (en) 2005-10-27 2007-05-03 Cythera, Inc. Pdx1-expressing dorsal and ventral foregut endoderm
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
JP6110048B2 (ja) 2006-03-02 2017-04-05 ヴィアサイト，インコーポレイテッド 内分泌前駆細胞、膵臓ホルモン発現細胞及びそれらの製造方法
JP5391488B2 (ja) * 2006-09-28 2014-01-15 ティシューテック・インコーポレイテッド 接着結合を持つ細胞の増殖を刺激するＲＮＡｉ方法および組成物
US7695963B2 (en) * 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
JP2012508584A (ja) 2008-11-14 2012-04-12 ヴィアサイト，インコーポレイテッド ヒト多能性幹細胞由来膵臓細胞のカプセル化
JP5709396B2 (ja) * 2010-03-30 2015-04-30 学校法人慶應義塾 がんワクチン
GB201220058D0 (en) * 2012-11-07 2012-12-19 Univ Manchester Cell differentiation
KR102277336B1 (ko) * 2015-01-29 2021-07-13 고쿠리츠다이가쿠호우진 도쿄다이가쿠 세포의 배양 방법, 세포의 응집체, 세포 응집 제어제, 및 배지
JP6949349B2 (ja) * 2016-12-20 2021-10-13 国立大学法人大阪大学 多能性幹細胞の増殖を促進するための組成物、及び多能性幹細胞の増殖促進方法
US10494345B2 (en) 2017-10-03 2019-12-03 Zonula, Inc. Modulators of cell adhesion, methods and compositions therefor

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1124976A1 (de) 1998-10-27 2001-08-22 Crucell Holland B.V. Verbesserte produktion von aav-vektoren
ES2161612B1 (es) 1999-07-01 2002-06-01 Consejo Superior Investigacion Procedimiento para identificar un compuesto que inhiba la funcion represora de snail.
WO2001075109A2 (en) 2000-03-31 2001-10-11 Imclone Systems Incorporated Antagonist antibodies to ve-cadherin without adverse effects on vascular permeability
AU2002226075A1 (en) 2000-10-23 2002-05-06 University Of Kansas Cadherin peptides for drug delivery and inhibition of tumor metastasis/invasion
US20030190704A1 (en) * 2002-04-04 2003-10-09 Ting Xie Methods and compositions for anchoring stem cells in a microenvironment
KR101178786B1 (ko) 2004-03-23 2012-09-07 다이이찌 산쿄 가부시키가이샤 다능성 줄기세포의 증식 방법

Also Published As

Publication number Publication date
EP1979470B1 (de) 2011-10-26
US8883501B2 (en) 2014-11-11
WO2007088372A2 (en) 2007-08-09
US20090155222A1 (en) 2009-06-18
WO2007088372A3 (en) 2007-11-15
GB0602063D0 (en) 2006-03-15
EP1979470A2 (de) 2008-10-15

Similar Documents

Publication Publication Date Title
ATE530636T1 (de) Zellkultur
GB2446525A (en) Stem cell culture medium and method
MX356756B (es) Células madre pluripotentes en microportadores.
AU2019268194A1 (en) Cell culture system
WO2006070370A3 (en) Stem cells culture systems
GB2436737B (en) Culture medium containing kinase inhibitors,and uses thereof
WO2007016485A3 (en) Use of a gsk-3 inhibitor to maintain potency of cultured cells
SG170761A1 (en) Methods for cell expansion and uses of cells and conditioned media produced thereby for therapy
MX338534B (es) Poblaciones de celula madre de la placenta.
EP2408903A4 (de) Pluripotente und multipotente zellkultur auf mikroträgern
MX2007001772A (es) Composiciones y metodos para auto-renovacion y diferenciacion de celulas troncales embrionicas humanas.
SG196784A1 (en) Stem cell cultures
BR112019009746A2 (pt) ensaio de células-tronco pluripotentes
EP1860180A4 (de) Verfahren zur amplifikation einer endothelvorläuferzelle in vitro
WO2004090096A3 (en) Methods for neural differentiation of embryonic stem cells using protease passaging techniques
DK1720979T3 (da) Anvendelse af et serumfrit celledyrkningsmedium til fremstilling af IL-18BP I pattedyrceller
WO2010020876A3 (en) Cell culture method to form aggregates
WO2005087915A3 (en) Use of ethanolamine for enhancing cell growth in membrane systems
DE602005026548D1 (de) Serumfreies kulturmedium für säugerzellen
ATE336567T1 (de) Zellkulturmedium
ATE527341T1 (de) Hepatische differenzierung von stammzellen
BRPI1008762B8 (pt) métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivo
WO2012046065A3 (en) Culture method for culturing pluripotent cells comprising an inhibitor of mirna- 181a*
MX2010005079A (es) Metodos para medir la viabilidad celular sin usar celulas de control.
ATE463582T1 (de) Screening-verfahren zur identifizierung neuer aminoacyl-trna-synthetase-inhibitoren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties