BRPI1008762B8 - métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivo - Google Patents
métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivoInfo
- Publication number
- BRPI1008762B8 BRPI1008762B8 BRPI1008762A BRPI1008762A BRPI1008762B8 BR PI1008762 B8 BRPI1008762 B8 BR PI1008762B8 BR PI1008762 A BRPI1008762 A BR PI1008762A BR PI1008762 A BRPI1008762 A BR PI1008762A BR PI1008762 B8 BRPI1008762 B8 BR PI1008762B8
- Authority
- BR
- Brazil
- Prior art keywords
- interest
- product
- culture medium
- selecting
- host cells
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
método para seleção de células eucarióticas expressando uma proteína heteróloga. a presente invenção refere-se a um método para selecionar pelo menos uma célula hospedeira eucariótica expressando um produto de interesse, compreendendo: (a) proporcionar uma pluralidade de células hospedeiras eucarióticas, em que a viabilidade de célula das ditas células hospedeiras depende da captação de folato, em que as ditas células hospedeiras incluem pelo menos (i) um polinucleotídeo estrangeiro que codifica um produto de interesse e (ii) um polinucleotídeo estrangeiro que codifica uma enzima da dhfr; (b) cultivar a dita pluralidade de células hospedeiras eucarióticas em um meio de cultura seletivo, compreendendo pelos menos um inibidor de dhfr e folato em uma concentração limitante, e (c) selecionar pelo menos uma célula hospedeira eucariótica expressando o produto de interesse. também é fornecido um método para expressar um produto de interesse que é baseado em células hospedeiras selecionadas por meio do dito método e um meio de cultura de célula.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09154000.5 | 2009-02-27 | ||
| EP09154000 | 2009-02-27 | ||
| PCT/EP2010/001223 WO2010097239A1 (en) | 2009-02-27 | 2010-02-26 | Methods for selecting eukaryotic cells expressing a heterologous protein |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BRPI1008762A2 BRPI1008762A2 (pt) | 2015-08-25 |
| BRPI1008762B1 BRPI1008762B1 (pt) | 2020-10-27 |
| BRPI1008762B8 true BRPI1008762B8 (pt) | 2021-05-25 |
Family
ID=40451117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI1008762A BRPI1008762B8 (pt) | 2009-02-27 | 2010-02-26 | métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivo |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US8962274B2 (pt) |
| EP (1) | EP2401383B1 (pt) |
| JP (2) | JP5745431B2 (pt) |
| KR (1) | KR101706399B1 (pt) |
| CN (1) | CN102333875B (pt) |
| AU (1) | AU2010219088B2 (pt) |
| BR (1) | BRPI1008762B8 (pt) |
| CA (1) | CA2753813C (pt) |
| DK (1) | DK2401383T3 (pt) |
| ES (1) | ES2440321T3 (pt) |
| IL (1) | IL214620A (pt) |
| MX (1) | MX2011009023A (pt) |
| PL (1) | PL2401383T3 (pt) |
| PT (1) | PT2401383E (pt) |
| RU (1) | RU2551229C2 (pt) |
| SG (1) | SG173457A1 (pt) |
| WO (1) | WO2010097239A1 (pt) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5801207B2 (ja) | 2009-02-27 | 2015-10-28 | ノバルティス アーゲー | ２種類の選択マーカーを含む発現ベクター系 |
| US8962274B2 (en) * | 2009-02-27 | 2015-02-24 | Novartis Ag | Methods for selecting eukaryotic cells expressing a heterologous protein |
| SG2014008809A (en) * | 2011-09-21 | 2014-04-28 | Hoffmann La Roche | Co2 profile cultivation |
| RU2716977C2 (ru) | 2013-07-31 | 2020-03-17 | Новартис Аг | Новые селективные векторы и способы селекции эукариотических клеток-хозяев |
| US20180066268A1 (en) * | 2015-04-03 | 2018-03-08 | Selexis S.A. | Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells |
| UY37758A (es) | 2017-06-12 | 2019-01-31 | Novartis Ag | Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2585532B2 (ja) | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| AU6432090A (en) | 1989-09-01 | 1991-04-08 | Genentech Inc. | Cells transfected with nucleic acid encoding gtp |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| AR008077A1 (es) | 1996-07-26 | 1999-12-09 | Talarico Salinas Laura Beatriz | Un polipeptido de fusion o una sal del mismo, su uso, un proceso para prepararlos, una composicion farmaceutica que los comprende, y un vector. |
| JP4031154B2 (ja) | 1999-07-30 | 2008-01-09 | 独立行政法人科学技術振興機構 | 遺伝子増幅細胞の迅速選択法 |
| EP1349873B1 (en) | 2000-09-14 | 2009-04-01 | Beth Israel Deaconess Medical Center, Inc. | Modulation of il-2- and il-15-mediated t cell responses |
| CA2466881A1 (en) * | 2001-11-28 | 2003-06-05 | Sandoz Gmbh | Cell culture process |
| US7384744B2 (en) * | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| TW200513351A (en) * | 2003-07-17 | 2005-04-16 | Availvs Corp | High pressure water jet surface cutting device and cutting method |
| EP1533617A1 (en) | 2003-11-19 | 2005-05-25 | RMF Dictagene S.A. | Angiogenesis inhibiting molecules, their selection, production and their use in the treatment and diagnosis of cancer |
| EP1953222A1 (en) | 2007-01-24 | 2008-08-06 | Boehringer Ingelheim Pharma GmbH & Co. KG | Improvement of cell growth |
| NZ580378A (en) * | 2007-03-30 | 2012-11-30 | Abbott Lab | Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells |
| US8962274B2 (en) * | 2009-02-27 | 2015-02-24 | Novartis Ag | Methods for selecting eukaryotic cells expressing a heterologous protein |
-
2010
- 2010-02-26 US US13/203,546 patent/US8962274B2/en active Active
- 2010-02-26 AU AU2010219088A patent/AU2010219088B2/en active Active
- 2010-02-26 KR KR1020117022540A patent/KR101706399B1/ko active Active
- 2010-02-26 PT PT107096877T patent/PT2401383E/pt unknown
- 2010-02-26 EP EP10709687.7A patent/EP2401383B1/en active Active
- 2010-02-26 ES ES10709687.7T patent/ES2440321T3/es active Active
- 2010-02-26 DK DK10709687.7T patent/DK2401383T3/da active
- 2010-02-26 MX MX2011009023A patent/MX2011009023A/es active IP Right Grant
- 2010-02-26 WO PCT/EP2010/001223 patent/WO2010097239A1/en not_active Ceased
- 2010-02-26 PL PL10709687T patent/PL2401383T3/pl unknown
- 2010-02-26 CN CN201080009517.7A patent/CN102333875B/zh active Active
- 2010-02-26 SG SG2011054087A patent/SG173457A1/en unknown
- 2010-02-26 JP JP2011551443A patent/JP5745431B2/ja active Active
- 2010-02-26 RU RU2011139183/10A patent/RU2551229C2/ru active
- 2010-02-26 CA CA2753813A patent/CA2753813C/en active Active
- 2010-02-26 BR BRPI1008762A patent/BRPI1008762B8/pt active IP Right Grant
-
2011
- 2011-08-11 IL IL214620A patent/IL214620A/en active IP Right Grant
-
2014
- 2014-02-06 US US14/174,228 patent/US9315844B2/en active Active
- 2014-12-24 JP JP2014261016A patent/JP2015107120A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| DK2401383T3 (da) | 2013-12-16 |
| WO2010097239A1 (en) | 2010-09-02 |
| IL214620A0 (en) | 2011-09-27 |
| EP2401383B1 (en) | 2013-09-18 |
| US20110306095A1 (en) | 2011-12-15 |
| PL2401383T3 (pl) | 2014-02-28 |
| EP2401383A1 (en) | 2012-01-04 |
| JP2012518991A (ja) | 2012-08-23 |
| IL214620A (en) | 2016-02-29 |
| CN102333875B (zh) | 2014-04-16 |
| ES2440321T3 (es) | 2014-01-28 |
| PT2401383E (pt) | 2013-12-16 |
| BRPI1008762A2 (pt) | 2015-08-25 |
| RU2551229C2 (ru) | 2015-05-20 |
| CA2753813A1 (en) | 2010-09-02 |
| JP2015107120A (ja) | 2015-06-11 |
| KR20110123783A (ko) | 2011-11-15 |
| US9315844B2 (en) | 2016-04-19 |
| KR101706399B1 (ko) | 2017-02-13 |
| SG173457A1 (en) | 2011-09-29 |
| MX2011009023A (es) | 2011-09-28 |
| AU2010219088B2 (en) | 2012-11-01 |
| US8962274B2 (en) | 2015-02-24 |
| JP5745431B2 (ja) | 2015-07-08 |
| BRPI1008762B1 (pt) | 2020-10-27 |
| AU2010219088A1 (en) | 2011-08-18 |
| CN102333875A (zh) | 2012-01-25 |
| US20140154739A1 (en) | 2014-06-05 |
| RU2011139183A (ru) | 2013-04-10 |
| CA2753813C (en) | 2018-12-11 |
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Legal Events
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| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
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| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
| B07B | Technical examination (opinion): publication cancelled [chapter 7.2 patent gazette] | ||
| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 27/10/2020, OBSERVADAS AS CONDICOES LEGAIS. |
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| B16C | Correction of notification of the grant [chapter 16.3 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 26/02/2010 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF |