ATE540128T1 - Verfahren und materialien unter anwendung von signalsonden - Google Patents

Verfahren und materialien unter anwendung von signalsonden

Info

Publication number
ATE540128T1
ATE540128T1 AT05713739T AT05713739T ATE540128T1 AT E540128 T1 ATE540128 T1 AT E540128T1 AT 05713739 T AT05713739 T AT 05713739T AT 05713739 T AT05713739 T AT 05713739T AT E540128 T1 ATE540128 T1 AT E540128T1
Authority
AT
Austria
Prior art keywords
methods
probes
signaling
structures
materials
Prior art date
Application number
AT05713739T
Other languages
English (en)
Inventor
Kambiz Shekdar
Dennis Sawchuk
Jason Montez
Original Assignee
Chromocell Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromocell Corp filed Critical Chromocell Corp
Application granted granted Critical
Publication of ATE540128T1 publication Critical patent/ATE540128T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Hydrogenated Pyridines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)
  • Variable-Direction Aerials And Aerial Arrays (AREA)
AT05713739T 2004-02-18 2005-02-17 Verfahren und materialien unter anwendung von signalsonden ATE540128T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54607504P 2004-02-18 2004-02-18
PCT/US2005/005080 WO2005079462A2 (en) 2004-02-18 2005-02-17 Methods and materials using signaling probes

Publications (1)

Publication Number Publication Date
ATE540128T1 true ATE540128T1 (de) 2012-01-15

Family

ID=34886236

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05713739T ATE540128T1 (de) 2004-02-18 2005-02-17 Verfahren und materialien unter anwendung von signalsonden

Country Status (16)

Country Link
US (2) US8916503B2 (de)
EP (3) EP2270204B1 (de)
JP (4) JP2007522818A (de)
KR (5) KR20100063144A (de)
CN (2) CN1981051A (de)
AT (1) ATE540128T1 (de)
AU (2) AU2005214970B2 (de)
CA (1) CA2556418C (de)
DE (1) DE10180022T1 (de)
DK (1) DK1725573T3 (de)
ES (1) ES2379634T3 (de)
HK (1) HK1204480A1 (de)
IL (1) IL177495A (de)
MX (1) MXPA06009423A (de)
NZ (3) NZ549622A (de)
WO (1) WO2005079462A2 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006138145A1 (en) 2005-06-14 2006-12-28 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
EP1975254A4 (de) 2006-01-20 2009-12-02 Olympus Corp Verfahren für den nachweis von nukleotidsequenzen mit einer intramolekularen sonde
MX2009005810A (es) * 2006-11-30 2009-08-13 Chromocell Corp Celulas hospederas optimizadas para la produccion de proteinas.
AU2008212820B2 (en) 2007-02-09 2014-01-30 Northwestern University Particles for detecting intracellular targets
JP5126877B2 (ja) * 2007-06-26 2013-01-23 独立行政法人理化学研究所 一塩基変異体の検出方法
NZ601353A (en) 2008-02-01 2014-06-27 Chromocell Corp Cell lines and methods for making and using them
US20100233270A1 (en) 2009-01-08 2010-09-16 Northwestern University Delivery of Oligonucleotide-Functionalized Nanoparticles
CN102369275A (zh) 2009-02-02 2012-03-07 卓莫赛尔公司 新型细胞系和方法
WO2010099662A1 (zh) * 2009-03-05 2010-09-10 厦门艾德生物医药科技有限公司 一种用于核酸实时检测的探针
US9034605B2 (en) * 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
US8945848B2 (en) 2009-07-31 2015-02-03 Chromocell Corporation Methods and compositions for identifying and validating modulators of cell fate
WO2011017288A1 (en) 2009-08-05 2011-02-10 Chromocell Corporation Improved plants, microbes, and organisms
US9139860B2 (en) * 2011-04-11 2015-09-22 Korea University Research And Business Foundation Signal-amplifying folded oligonucleotide
CN104023749B (zh) 2011-09-14 2019-12-17 西北大学 能穿过血脑屏障的纳米缀合物
US9347934B2 (en) 2011-10-20 2016-05-24 Chromocell Corporation Assays for identifying compounds that modulate bitter taste
EP2653556B1 (de) * 2012-04-20 2015-09-02 Alacris Theranostics GmbH CDR-sequenzspezifische Anreicherung lebender Immunzellen
WO2014151994A1 (en) 2013-03-15 2014-09-25 Kambiz Shekdar Genome editing using effector oligonucleotides for therapeutic treatment
WO2014144991A1 (en) * 2013-03-15 2014-09-18 Chromocell Corporation Methods and materials using signaling probes
WO2014176336A1 (en) 2013-04-24 2014-10-30 Chromocell Corporation Assays for identifying compounds that modulate bitter taste
WO2016081911A2 (en) 2014-11-21 2016-05-26 Northwestern University The sequence-specific cellular uptake of spherical nucleic acid nanoparticle conjugates
CN114350752B (zh) 2015-07-17 2025-02-25 布鲁克空间生物学公司 在横切面组织的用户定义区域中的基因表达的同时定量
CN105349683B (zh) * 2015-12-09 2018-12-18 山东大学 目标物保护的哑铃分子探针介导的级联滚环扩增策略用于dna甲基转移酶活性的灵敏检测
WO2018039629A2 (en) 2016-08-25 2018-03-01 Northwestern University Micellar spherical nucleic acids from thermoresponsive, traceless templates
AU2017361521B2 (en) * 2016-11-21 2020-08-27 Bruker Spatial Biology, Inc. Chemical compositions and methods of using same
US20210293783A1 (en) 2017-04-18 2021-09-23 The General Hospital Corporation Compositions for detecting secretion and methods of use
JP6788148B1 (ja) * 2017-10-23 2020-11-18 ザ ユナイテッド ステイツ オブ アメリカ, アズ リプレゼンテッド バイ ザ セクレタリー, デパートメント オブ ヘルス アンド ヒューマン サービシーズ スペクトル散乱フローサイトメトリのための光学構成方法
CN108531566A (zh) * 2018-04-17 2018-09-14 厦门基科生物科技有限公司 基于复合编码探针杂交的dna检测方法及复合编码探针
US12281301B2 (en) 2018-04-27 2025-04-22 The Broad Institute, Inc. Sequencing-based proteomics
KR102893736B1 (ko) 2018-05-14 2025-11-28 브루커 스페이셜 바이올로지, 인크. 화학 조성물 및 이의 사용 방법
EP4277992A4 (de) * 2021-01-15 2025-03-26 Secondcell Bio, Llc Zellmanipulationszusammensetzungen und verfahren mit fluorogenen oligonukleotidsignalisierungssonden und durchflusszytometrie
CN117467751B (zh) * 2023-12-27 2024-03-29 北京百力格生物科技有限公司 靶向目的基因fish荧光探针及其自组装放大探针系统
CN121046524A (zh) * 2024-05-30 2025-12-02 上海之江生物科技股份有限公司 一种结构探针及其应用

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991007660A1 (en) 1989-11-13 1991-05-30 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal dna
ATE515510T1 (de) 1991-12-24 2011-07-15 Isis Pharmaceuticals Inc Durch dna-abschnitte unterbrochene modifizierte oligonukleotide
US5629147A (en) * 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
WO1994002646A1 (en) 1992-07-17 1994-02-03 Aprogenex Inc. Enriching and identyfying fetal cells in maternal blood for in situ hybridization
EP0689460A4 (de) 1993-02-19 1996-11-13 Isis Pharmaceuticals Inc Cyclobutyl antisinn-oligonukleotide, herstellungsmethoden und verwendung davon
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
EP0760008A1 (de) 1994-05-19 1997-03-05 Dako A/S Pna-sonden zum nachweis von neisseria gonorrhoeae und chlamydia trachomatis
US5641675A (en) * 1994-10-07 1997-06-24 University Of Massachusetts Medical Center Cis-acting sequences for intracellular localization of RNA
CA2202549C (en) 1994-10-14 2003-08-05 Tara Seshadri Transgenic nonhuman animal having functionally disrupted interleukin-1.beta. converting enzyme gene
US5783683A (en) * 1995-01-10 1998-07-21 Genta Inc. Antisense oligonucleotides which reduce expression of the FGFRI gene
US5741657A (en) * 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
US5731148A (en) 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US7098320B1 (en) * 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
GB9701492D0 (en) 1997-01-24 1997-03-12 Univ Edinburgh Transfection of embryonic stem cells
US6416959B1 (en) * 1997-02-27 2002-07-09 Kenneth Giuliano System for cell-based screening
US6750052B1 (en) * 1997-07-23 2004-06-15 The Brigham And Women's Hospital, Inc. Lens epithelial cell derived growth factor
AU9220498A (en) 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU3909199A (en) * 1997-12-15 1999-07-05 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
WO1999037806A2 (en) * 1998-01-27 1999-07-29 Cytocell Limited Modified nucleic acid probes and uses thereof
KR20010042167A (ko) * 1998-03-24 2001-05-25 보스턴 프로브스, 인코포레이티드 검출 복합체에 관한 방법, 키트 및 조성물
WO1999053017A2 (en) * 1998-04-15 1999-10-21 Fred Hutchinson Cancer Research Center Methods and vector constructs for making transgenic non-human animals which ubiquitously express a heterologous gene
EP1092047B1 (de) * 1998-07-02 2009-08-26 Gen-Probe Incorporated Molekulare fackeln
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6203986B1 (en) * 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
AU1827200A (en) 1998-11-25 2000-06-13 Isis Pharmaceuticals, Inc. (in situ) binary synthesis of biologically effective molecules
WO2000043552A2 (en) * 1999-01-25 2000-07-27 Ut-Battelle, Llc Multifunctional and multispectral biosensor devices and methods of use
EP1175616A4 (de) 1999-04-13 2003-08-13 Northwest Biotherapeutics Inc Verfahren für die diagnose und die behandlung von metastatischen prostatatumoren
GB2355791B (en) 1999-10-28 2004-10-20 Molecular Light Tech Res Ltd Detection of mRNA expression using chemiluminescent labelled probes
US6692965B1 (en) * 1999-11-23 2004-02-17 Chromocell Corporation Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences
CN1180091C (zh) * 1999-12-08 2004-12-15 中国人民解放军军事医学科学院放射医学研究所 一种复合基因探针的结构和用途
JP3460673B2 (ja) * 2000-02-04 2003-10-27 浜松ホトニクス株式会社 特定の遺伝子を発現した生細胞の選択的分離方法
EP1172445A1 (de) 2000-07-14 2002-01-16 Praenadia GmbH Verfahren zur direkten genetischen Analyse von Zielzellen anhand Fluoreszentensonden
WO2002018951A2 (en) * 2000-08-29 2002-03-07 The Rockefeller University Methods employing fluorescence quenching by metal surfaces
AU2002213096B2 (en) 2000-10-06 2007-03-29 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
WO2002061133A2 (en) 2000-11-09 2002-08-08 Yale University Nucleic acid detection using structured probes
IL156200A0 (en) 2000-12-22 2003-12-23 Univ Edinburgh Thymic epithelial progenitor cells and pharmaceutical compositions containing the same
US20020090614A1 (en) 2001-01-09 2002-07-11 Jia Zhang Direct measurement method for gene expression using single chain antisense array
JP4537053B2 (ja) * 2001-06-25 2010-09-01 ジョージア テック リサーチ コーポレーション 二重共鳴エネルギー転移核酸プローブ
US7070933B2 (en) * 2001-09-28 2006-07-04 Gen-Probe Incorporated Inversion probes
CA2438297A1 (en) 2001-12-04 2003-06-12 Genome Biosciences, Llc Gene targeting methods and vectors
US20040086879A1 (en) * 2001-12-20 2004-05-06 Yingufu Li Tripartite molecular beacons
EP1405908A1 (de) 2002-10-04 2004-04-07 ProBioGen AG Herstellung von Zelllinien zur heterologen Expression
CN1206368C (zh) * 2003-03-05 2005-06-15 东南大学 固相化核酸检测探针及其制备方法
AU2008212820B2 (en) * 2007-02-09 2014-01-30 Northwestern University Particles for detecting intracellular targets

Also Published As

Publication number Publication date
HK1102512A1 (en) 2007-11-23
CN1981051A (zh) 2007-06-13
KR20060129469A (ko) 2006-12-15
KR20130082179A (ko) 2013-07-18
WO2005079462A3 (en) 2007-02-01
KR20160045937A (ko) 2016-04-27
KR20100063144A (ko) 2010-06-10
US20090106853A1 (en) 2009-04-23
KR20150005720A (ko) 2015-01-14
EP1725573B1 (de) 2012-01-04
EP1725573A2 (de) 2006-11-29
EP2270204A3 (de) 2011-08-24
US8916503B2 (en) 2014-12-23
DE10180022T1 (de) 2011-12-01
MXPA06009423A (es) 2007-04-02
CA2556418C (en) 2017-09-26
NZ597400A (en) 2013-06-28
NZ549622A (en) 2010-09-30
KR101771373B1 (ko) 2017-09-05
EP1725573A4 (de) 2007-08-01
AU2005214970B2 (en) 2010-07-29
JP2015097532A (ja) 2015-05-28
JP2007522818A (ja) 2007-08-16
NZ586619A (en) 2012-02-24
AU2010236074A1 (en) 2010-11-18
CN104109712A (zh) 2014-10-22
IL177495A0 (en) 2008-03-20
ES2379634T3 (es) 2012-04-30
EP2806035B1 (de) 2017-09-13
AU2010236074B2 (en) 2012-09-27
WO2005079462A2 (en) 2005-09-01
JP2010246570A (ja) 2010-11-04
DK1725573T3 (da) 2012-04-02
AU2005214970A1 (en) 2005-09-01
EP2806035A1 (de) 2014-11-26
HK1150634A1 (en) 2012-01-06
US20150299782A1 (en) 2015-10-22
EP2270204A2 (de) 2011-01-05
CA2556418A1 (en) 2005-09-01
EP2270204B1 (de) 2014-05-28
IL177495A (en) 2013-08-29
JP2013078320A (ja) 2013-05-02
HK1204480A1 (en) 2015-11-20

Similar Documents

Publication Publication Date Title
DK1725573T3 (da) Fremgangsmåde og materialer ved anvendelse af signalsonder
Trichas et al. Use of the viral 2A peptide for bicistronic expression in transgenic mice
ATE498011T1 (de) Modifizierte embryonale stammzellen und embryonales stammzellspezifisches gen
Shaw Interspecific genetics of mate recognition: inheritance of female acoustic preference in Hawaiian crickets
AR093045A2 (es) Un metodo de aplicar un herbicida a una planta transgenica para producir una semilla hibrida no natural transgenica, celulas no naturales y adn recombinante
NZ538852A (en) Selection and isolation of living cells using mRNA-binding probes
EP1750771A4 (de) Pankreasinsel-mikro-rna und verfahren zu ihrer unterdrückung
ATE528402T1 (de) Effiziente genabschaltung in pflanzen unter verwendung von kurzen dsrna-sequenzen
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
ATE401397T1 (de) Gerichtete evolution von biomolekülen unter der verwendung von mikrostrukturen
Fischer et al. The enigma of interspecific plasmodesmata: insight from parasitic plants
Pimanda et al. Endoglin expression in blood and endothelium is differentially regulated by modular assembly of the Ets/Gata hemangioblast code
ATE387497T1 (de) Samenspezifische 7s-alpha-promoter zur expression von genen in pflanzen
DE69630142D1 (de) Verfahren zur identifizierung metastatischer sequenzen
DE50313671D1 (de) Verfahren zur generierung eines gentechnisch veränderten organismus für das wirksubstanzscreening
ATE414701T1 (de) Fluoreszenzsonden zur verwendung bei einem bindungsassay auf proteinkinaseinhibitoren
ATE256120T1 (de) Reagentien für cyp2d fluoreszenztest
Williams et al. The math of making mutant mice
US10386361B2 (en) Long term hematopoietic stem cell specific reporter mouse and uses thereof
Hughes et al. A novel single vector intersectional aav strategy for interrogating cellular diversity and brain function
ATE406093T1 (de) Verfahren und zusammensetzungen für die pharmakologische und toxikologische auswertung von testsubstanzen
McCammon et al. Using zinc finger nucleases for efficient and heritable gene disruption in zebrafish
ATE549421T1 (de) Zusammensetzungen, verfahren und kits zum nachweis der nukleinsäuren von hiv-1 und hiv-2
WO2006005043A3 (en) Compositions and methods for detecting protein interactions with target dna sequences
DE602005027414D1 (de) Verfahren zur übertragung episomaler vektoren in tierzellen