ATE541046T1 - Verfahren zur entkopplung des reaktionsmassstabs und der proteinsyntheseausbeute im diskontinuierlichen modus - Google Patents

Verfahren zur entkopplung des reaktionsmassstabs und der proteinsyntheseausbeute im diskontinuierlichen modus

Info

Publication number
ATE541046T1
ATE541046T1 AT04778237T AT04778237T ATE541046T1 AT E541046 T1 ATE541046 T1 AT E541046T1 AT 04778237 T AT04778237 T AT 04778237T AT 04778237 T AT04778237 T AT 04778237T AT E541046 T1 ATE541046 T1 AT E541046T1
Authority
AT
Austria
Prior art keywords
decoupling
protein synthesis
discontinuous mode
reaction scale
synthesis yield
Prior art date
Application number
AT04778237T
Other languages
English (en)
Inventor
Alexei Voloshin
James Swartz
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Application granted granted Critical
Publication of ATE541046T1 publication Critical patent/ATE541046T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT04778237T 2003-07-18 2004-07-12 Verfahren zur entkopplung des reaktionsmassstabs und der proteinsyntheseausbeute im diskontinuierlichen modus ATE541046T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48828203P 2003-07-18 2003-07-18
PCT/US2004/022632 WO2005010155A2 (en) 2003-07-18 2004-07-12 Methods of decoupling reaction scale and protein synthesis yield in batch mode

Publications (1)

Publication Number Publication Date
ATE541046T1 true ATE541046T1 (de) 2012-01-15

Family

ID=34102758

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04778237T ATE541046T1 (de) 2003-07-18 2004-07-12 Verfahren zur entkopplung des reaktionsmassstabs und der proteinsyntheseausbeute im diskontinuierlichen modus

Country Status (6)

Country Link
US (1) US7341852B2 (de)
EP (1) EP1649025B1 (de)
JP (1) JP4751829B2 (de)
AT (1) ATE541046T1 (de)
AU (1) AU2004259433B2 (de)
WO (1) WO2005010155A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7351563B2 (en) 2005-06-10 2008-04-01 The Board Of Trustees Of The Leland Stanford Junior University Cell-free extracts and synthesis of active hydrogenase
US7312049B2 (en) 2005-06-14 2007-12-25 The Board Of Trustees Of The Leland Stanford Junior University Total amino acid stabilization during cell-free protein synthesis
CA2626061A1 (en) 2005-10-31 2007-05-10 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of membrane bound polypeptides
US20110129438A1 (en) * 2006-06-28 2011-06-02 James Robert Swartz Immunogenic protein constructs
WO2008002661A2 (en) * 2006-06-28 2008-01-03 The Board Of Trustees Of The Leland Stanford Junior University Fusion protein constructs
DK2035554T3 (da) * 2006-06-29 2013-06-17 Univ Leland Stanford Junior Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer
US20090317861A1 (en) * 2006-06-29 2009-12-24 Bundy Bradley C Cell-free synthesis of virus like particles
US20100168402A1 (en) * 2008-11-14 2010-07-01 Bundy Bradley C Direct Attachment of Polypeptides to Virus Like Particles
WO2010077806A1 (en) 2008-12-15 2010-07-08 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
CN102333878B (zh) * 2008-12-22 2015-08-26 绿光生物科学公司 用于制造化合物的组合物和方法
KR20130071433A (ko) 2010-05-07 2013-06-28 그린라이트 바이오사이언시스, 아이엔씨. 효소의 재배치를 통한 대사 경로에서의 흐름의 제어 방법
US8916358B2 (en) 2010-08-31 2014-12-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
CN104093848A (zh) 2011-09-09 2014-10-08 绿光生物科学公司 碳青霉烯(carbapenem)的无细胞制备
US9296792B2 (en) 2012-05-23 2016-03-29 The Board Of Trustees Of The Leland Stanford Junior University Ordered flagellin array as an immunostimulant
JP2016507520A (ja) 2013-01-23 2016-03-10 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 安定化されたｂ型肝炎コアポリペプチド
US9255255B2 (en) 2013-03-04 2016-02-09 The Board Of Trustees Of The Leland Stanford Junior University Synthesis of linear and branched polymers of polypeptides through direct conjugation
WO2015021058A2 (en) 2013-08-05 2015-02-12 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
RU2017137788A (ru) 2015-03-30 2019-04-30 Гринлайт Байосайенсис, Инк. Бесклеточное получение рибонуклеиновой кислоты
US10221401B2 (en) 2015-09-08 2019-03-05 The Board Of Trustees Of The Leland Stanford Junior University Oxygen tolerant hydrogenase by mutating electron supply pathway
SG11201808721YA (en) 2016-04-06 2018-11-29 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
IL267709B2 (en) 2016-12-30 2025-04-01 Vaxcyte Inc Polypeptide-antigen conjugates with unnatural amino acids
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
KR20190100386A (ko) 2017-01-06 2019-08-28 그린라이트 바이오사이언시스, 아이엔씨. 당의 무세포 생산
CN106834391A (zh) * 2017-01-18 2017-06-13 天津大学 一种基于人工多酶生化反应网络合成蛋白质的配方和方法
WO2019075167A1 (en) 2017-10-11 2019-04-18 Greenlight Biosciences, Inc. METHODS AND COMPOSITIONS FOR THE PRODUCTION OF NUCLEOSIDE TRIPHOSPHATE AND RIBONUCLEIC ACID
FR3076062B1 (fr) 2017-12-21 2020-07-17 Commissariat A L'energie Atomique Et Aux Energies Alternatives Realisation d'un collecteur de dispositif microelectronique
US11821018B2 (en) 2020-02-14 2023-11-21 Nuclera Ltd. Methods for cell-free protein expression
GB202013063D0 (en) 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Real-time monitoring of in vitro protein synthesis

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6337191B1 (en) * 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
RU2169154C2 (ru) * 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
US7485454B1 (en) * 2000-03-10 2009-02-03 Bioprocessors Corp. Microreactor
US20040147014A1 (en) * 2001-07-19 2004-07-29 Koji Ikuta Chemical reaction circuit for cell-free protein synthesis
PT1539948E (pt) 2002-08-19 2010-01-20 Univ Leland Stanford Junior Métodos melhorados para a síntese de proteínas in vitro
WO2005098048A1 (en) 2004-03-25 2005-10-20 The Board Of Trustees Of The Leland Stanford Junior University Protein expression yield enhancement in cell-free protein synthesis systems by addition of antifoam agents

Also Published As

Publication number Publication date
AU2004259433A1 (en) 2005-02-03
EP1649025A2 (de) 2006-04-26
JP4751829B2 (ja) 2011-08-17
AU2004259433B2 (en) 2010-06-17
EP1649025B1 (de) 2012-01-11
US7341852B2 (en) 2008-03-11
WO2005010155A3 (en) 2005-04-28
JP2006527997A (ja) 2006-12-14
EP1649025A4 (de) 2007-07-25
US20050054032A1 (en) 2005-03-10
WO2005010155A2 (en) 2005-02-03

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