ATE542894T1 - Ausweitung des eukaryotischen genetischen codes - Google Patents

Ausweitung des eukaryotischen genetischen codes

Info

Publication number
ATE542894T1
ATE542894T1 AT04759917T AT04759917T ATE542894T1 AT E542894 T1 ATE542894 T1 AT E542894T1 AT 04759917 T AT04759917 T AT 04759917T AT 04759917 T AT04759917 T AT 04759917T AT E542894 T1 ATE542894 T1 AT E542894T1
Authority
AT
Austria
Prior art keywords
amino acids
expansion
genetic code
eukaryotic genetic
orthogonal
Prior art date
Application number
AT04759917T
Other languages
English (en)
Inventor
Jason Chin
Ashton Cropp
Christopher Anderson
Peter Schultz
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Application granted granted Critical
Publication of ATE542894T1 publication Critical patent/ATE542894T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01001—Tyrosine-tRNA ligase (6.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/0102—Phenylalanine-tRNA ligase (6.1.1.20)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT04759917T 2003-04-17 2004-04-16 Ausweitung des eukaryotischen genetischen codes ATE542894T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US46386903P 2003-04-17 2003-04-17
US47993103P 2003-06-18 2003-06-18
US49301403P 2003-08-05 2003-08-05
US49654803P 2003-08-19 2003-08-19
PCT/US2004/011786 WO2004094593A2 (en) 2003-04-17 2004-04-16 Expanding the eukaryotic genetic code

Publications (1)

Publication Number Publication Date
ATE542894T1 true ATE542894T1 (de) 2012-02-15

Family

ID=33314444

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04759917T ATE542894T1 (de) 2003-04-17 2004-04-16 Ausweitung des eukaryotischen genetischen codes

Country Status (11)

Country Link
US (10) US7618775B2 (de)
EP (1) EP1613735B1 (de)
JP (2) JP5642916B2 (de)
KR (1) KR101171397B1 (de)
CN (4) CN103215227B (de)
AT (1) ATE542894T1 (de)
AU (2) AU2004233083B2 (de)
CA (1) CA2520750A1 (de)
IL (1) IL171128A (de)
MX (1) MXPA05011127A (de)
WO (1) WO2004094593A2 (de)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2322631T3 (en) * 2001-04-19 2015-01-12 Scripps Research Inst Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA syntetasepar
CA2500653A1 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
KR101171397B1 (ko) 2003-04-17 2012-08-07 더 스크립스 리서치 인스티튜트 진핵 유전자 코드의 확장
CA2527877A1 (en) 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
EP1658366A4 (de) 2003-07-07 2006-08-09 Scripps Research Inst Zusammensetzungen von orthogonalen glutamyl-trna- und aminoacyl-trna-synthetasepaaren und deren verwendungen
WO2005007870A2 (en) * 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
DK1687401T3 (da) 2003-10-14 2013-02-04 Scripps Research Inst Sted-spicifik inkorporing af redoxaktive aminosyrer i protiener
US8778880B2 (en) * 2004-02-02 2014-07-15 Ambrx, Inc. Human growth hormone modified at position 35
MXPA06013586A (es) * 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
KR101699142B1 (ko) * 2004-06-18 2017-01-23 암브룩스, 인코포레이티드 신규 항원-결합 폴리펩티드 및 이의 용도
US7638299B2 (en) 2004-07-21 2009-12-29 Ambrx, Inc. Biosynthetic polypeptides utilizing non-naturally encoded amino acids
ES2666693T3 (es) 2004-09-21 2018-05-07 The Scripps Research Institute Incorporación in vivo de alquinil aminoácidos a proteínas en eubacterias
WO2006036779A2 (en) * 2004-09-22 2006-04-06 The Scripps Research Institute Site-specific labeling of proteins for nmr studies
WO2006045116A2 (en) 2004-10-20 2006-04-27 The Scripps Research Institute In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
WO2006110182A2 (en) 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
JP2008035702A (ja) * 2004-11-26 2008-02-21 Institute Of Physical & Chemical Research 蛋白質間相互作用の検出方法
EP2284191A3 (de) * 2004-12-22 2011-07-20 Ambrx, Inc. Prozess zur Herstellung von hGH
US8080391B2 (en) * 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
GB0428012D0 (en) 2004-12-22 2005-01-26 Hammersmith Imanet Ltd Radiolabelling methods
WO2006068802A2 (en) 2004-12-22 2006-06-29 Ambrx, Inc. COMPOSITIONS OF AMINOACYL-tRNA SYNTHETASE AND USES THEREOF
WO2006071840A2 (en) 2004-12-22 2006-07-06 Ambrx, Inc. Formulations of human growth hormone comprising a non-naturally encoded amino acid
MX2007007590A (es) 2004-12-22 2007-12-10 Ambrx Inc Composiciones que contienen, metodos que involucran y usos de aminoacidos no naturales y polipeptidos.
GB0507123D0 (en) * 2005-04-08 2005-05-11 Isis Innovation Method
CA2609205A1 (en) * 2005-06-03 2006-12-14 Ambrx, Inc. Improved human interferon molecules and their uses
US8367588B2 (en) * 2005-10-12 2013-02-05 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
US20090018029A1 (en) 2005-11-16 2009-01-15 Ambrx, Inc. Methods and Compositions Comprising Non-Natural Amino Acids
ES2529065T3 (es) 2005-12-14 2015-02-16 Ambrx, Inc. Composiciones que contienen, procedimientos que implican y usos de aminoácidos no naturales y polipéptidos
AU2006330947B2 (en) 2005-12-22 2012-04-12 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US20070178554A1 (en) * 2006-02-01 2007-08-02 Nima Shiva Orthogonal Aminoacyl Synthetase-tRNA Pairs for Incorporating Unnatural Amino Acids Into Proteins
US20090081669A1 (en) * 2006-02-01 2009-03-26 Encode Bio, Inc. Fluorescent Assays Using Orthogonal tRNA - Aminoacyl Synthetase Pairs
US7776535B2 (en) * 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
ES2432391T3 (es) * 2006-03-09 2013-12-03 The Scripps Research Institute Sistema para la expresión de componentes de traducción ortogonales en células huésped eubacterianas
BRPI0709603A2 (pt) 2006-03-16 2011-07-19 Scripps Research Inst expressão de proteìnas geneticamente programada contendo o aminoácido não natural fenilselenocisteìna
CA2652894A1 (en) * 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
DK2615108T3 (en) * 2006-09-08 2017-01-30 Ambrx Inc Modified human plasma polypeptide or fc scaffolds and their applications
DK2064333T3 (da) 2006-09-08 2014-05-05 Ambrx Inc Suppressor-trna-transkription i hvirveldyrceller
NZ574673A (en) * 2006-09-08 2012-02-24 Ambrx Inc Site specific incorporation of non-natural amino acids by vertebrate cells
EP2052083B1 (de) 2006-09-21 2014-01-08 The Scripps Research Institute Genetisch programmierte expression von selektiv sulfatierten proteinen in eubakterien
EP2069481A4 (de) 2006-10-18 2010-01-06 Scripps Research Inst Genetischer einbau unnatürlicher aminosäuren in proteine in säugerzellen
PL2068909T3 (pl) 2007-03-30 2012-09-28 Ambrx Inc Modyfikowane polipeptydy fgf-21 i ich zastosowanie
EP2076533B1 (de) 2007-05-02 2014-10-08 Ambrx, Inc. Modifizierte interferon-beta-polypeptide und ihre verwendungen
CA2703736A1 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
US20090148887A1 (en) * 2007-11-02 2009-06-11 The Scripps Research Institute Genetically encoded boronate amino acid
CA2704494A1 (en) * 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
US8946148B2 (en) 2007-11-20 2015-02-03 Ambrx, Inc. Modified insulin polypeptides and their uses
EP2889370A1 (de) * 2007-12-11 2015-07-01 The Scripps Research Institute Unnatürliche In-vivo-Aminosäure-Expression bei der Methylotrophen-Hefe Pichia pastoris
JP2011514322A (ja) * 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
US9938333B2 (en) 2008-02-08 2018-04-10 Ambrx, Inc. Modified leptin polypeptides and their uses
CA2720046C (en) 2008-03-31 2018-07-24 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
PL2318029T3 (pl) 2008-07-23 2018-03-30 Elanco Us Inc. Zmodyfikowane polipeptydy bydlęcego G-CSF i ich zastosowania
US8278418B2 (en) 2008-09-26 2012-10-02 Ambrx, Inc. Modified animal erythropoietin polypeptides and their uses
CA2738033C (en) 2008-09-26 2019-11-26 Ambrx, Inc. Non-natural amino acid replication-dependent microorganisms and vaccines
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
JP5959201B2 (ja) * 2008-12-10 2016-08-02 ザ スクリプス リサーチ インスティテュート 化学的に反応性の非天然アミノ酸を使用するキャリア−ペプチド接合体の製造
BR112012015461A2 (pt) 2009-12-21 2017-01-10 Ambrx Inc polipeptídeos de somatotropina boviina modificados e seus usos
US20120283172A1 (en) 2009-12-21 2012-11-08 Ambrx, Inc. Modified porcine somatotropin polypeptides and their uses
EP3572091B1 (de) 2010-08-17 2023-12-13 Ambrx, Inc. Modifizierte relaxinpolypeptide und deren verwendungen
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
AU2011326181C1 (en) * 2010-11-10 2017-06-29 Nigel L. Webb Nuclions and ribocapsids
WO2012158945A2 (en) * 2011-05-19 2012-11-22 The Scripps Research Institute Compositions and methods for treating charcot-marie-tooth diseases and related neuronal diseases
CN102925427B (zh) * 2011-08-08 2014-01-22 中国科学院生物物理研究所 丙烯酰赖氨酸翻译系统及其应用
WO2014044873A1 (en) * 2012-09-24 2014-03-27 Allozyne, Inc Amino acid derivatives
CN102925476B (zh) * 2012-11-15 2014-12-03 中国科学院上海巴斯德研究所 酵母中自组装的具有免疫原性的肠道病毒病毒样颗粒的制备方法及其应用
UY35368A (es) 2013-03-08 2014-10-31 Irm Llc Péptidos y composiciones para el tratamiento de daño articular
US10005838B2 (en) 2013-07-19 2018-06-26 The Regents Of The University Of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
EP3044591B1 (de) * 2013-09-12 2018-08-29 LanzaTech New Zealand Limited Rekombinante mikroorganismen und verfahren zur verwendung davon
US9834775B2 (en) 2013-09-27 2017-12-05 President And Fellows Of Harvard College Recombinant cells and organisms having persistent nonstandard amino acid dependence and methods of making them
US10316322B2 (en) 2014-07-02 2019-06-11 Sutro Biopharma, Inc. High growth capacity auxotrophic Escherichia coli and methods of use
CN105420203B (zh) * 2014-09-03 2018-11-16 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN107108710B (zh) 2014-10-24 2022-02-15 百时美施贵宝公司 修饰的fgf-21多肽及其用途
ES2986970T3 (es) 2014-12-04 2024-11-13 Celgene Corp Conjugados de biomoléculas
WO2017079272A2 (en) * 2015-11-03 2017-05-11 Ambrx, Inc. Anti-cd3-folate conjugates and their uses
JP2018534943A (ja) * 2015-11-30 2018-11-29 ヨーロピアン モレキュラー バイオロジー ラボラトリーＥｕｒｏｐｅａｎ Ｍｏｌｅｃｕｌａｒ Ｂｉｏｌｏｇｙ Ｌａｂｏｒａｔｏｒｙ 昆虫細胞において遺伝暗号拡張によって遺伝子操作されたタンパク質を調製するための手段および方法
EP3538546B1 (de) 2016-11-14 2025-01-08 Novartis AG Zusammensetzungen, methoden und therapeutische verwendungen des fusogenen proteins minion
CN117860868A (zh) 2016-11-14 2024-04-12 诺华股份有限公司 用于治疗软骨损伤和关节炎的方法和组合物
PE20191716A1 (es) 2017-02-08 2019-12-05 Bristol Myers Squibb Co Polipeptidos de relaxina modificada que comprenden un mejorador farmacocinetico y sus usos
CN107119000B (zh) * 2017-04-19 2019-04-12 山东大学 荧光假单胞菌突变菌株的筛选方法及其在生物防治中的应用
TW202426655A (zh) 2017-07-11 2024-07-01 美商新索思股份有限公司 非天然核苷酸之導入及其方法
MA49767A (fr) 2017-08-03 2021-05-26 Synthorx Inc Conjugués de cytokine pour le traitement de maladies auto-immunes
AU2019263303A1 (en) 2018-05-01 2020-12-24 Ambrx, Inc. A method for optimizing antibody expression
EP4582440A3 (de) * 2018-12-21 2025-10-08 The Regents of the University of California Il-10-haltige impfstoffe und verwendungen davon
CA3127689A1 (en) 2019-02-06 2020-08-13 Synthorx, Inc. Il-2 conjugates and methods of use thereof
CN110172467B (zh) * 2019-05-24 2021-03-16 浙江大学 一种利用嵌合设计方法构建正交的氨酰-tRNA合成酶/tRNA体系
IL288941B2 (en) 2019-06-14 2025-10-01 Scripps Research Inst Reagents and methods for replication, transcription, and translation in semisynthetic organisms
WO2021072129A2 (en) 2019-10-08 2021-04-15 Trustees Of Boston College Proteins containing multiple, different unnatural amino acids and methods of making and using such proteins
KR20220080101A (ko) * 2019-10-15 2022-06-14 트러스티스 오브 보스톤 칼리지 향상된 비천연 아미노산 혼입을 위한 키메라 열안정성 아미노아실-tRNA 합성효소
CN115038789A (zh) 2019-12-02 2022-09-09 塑造治疗公司 治疗性编辑
CN115867648A (zh) * 2020-02-14 2023-03-28 西北大学 将用于遗传密码重编程的化学底物扩展至包括长链碳和环状氨基酸
CN111996206B (zh) * 2020-06-19 2023-01-31 清华大学 光控无细胞蛋白质合成方法、该方法使用的质粒以及使用该方法的产品
CN113481228B (zh) * 2021-06-13 2024-07-19 中国药科大学 微生物非天然氨基酸引入体系及构建方法
JP2024525866A (ja) 2021-07-16 2024-07-12 アプター バイオ，インク． 遺伝子発現を調節するためのポリヌクレオチド組成物および方法
US20240384267A1 (en) 2021-09-20 2024-11-21 The Broad Institute, Inc. Compositions and methods for multiplex decoding of quadruplet codons
CN115261344B (zh) * 2022-08-29 2023-07-21 北京大学 基于非天然氨基酸的离子液体、其制备方法及应用
WO2024091824A1 (en) 2022-10-26 2024-05-02 Ada Forsyth Institute, Inc. Differentiation and reprogramming of chondrocyte
CN117292741A (zh) * 2023-01-18 2023-12-26 浙江大学绍兴研究院 一种利用计算辅助设计开发结合细胞膜和/或血清白蛋白脂质化类似物的方法和应用
CN120752342A (zh) * 2023-07-13 2025-10-03 深圳华大生命科学研究院 工程生物遏制系统及方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE9115660U1 (de) 1991-12-18 1992-07-30 Aventis Research & Technologies GmbH & Co KG, 65929 Frankfurt L-Phenylalanyl-tRNA-Synthetase mit erweiterter Substratselektivität aus Mikroorganismen
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
AU4218599A (en) * 1998-05-27 1999-12-13 Scripps Research Institute, The Methods and systems for predicting protein function
WO2001070955A2 (en) * 2000-03-21 2001-09-27 Elitra Pharmaceuticals, Inc. Identification of essential genes in prokaryotes
WO2002077183A2 (en) * 2001-03-21 2002-10-03 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
DK2322631T3 (en) 2001-04-19 2015-01-12 Scripps Research Inst Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA syntetasepar
JP4263598B2 (ja) * 2001-08-01 2009-05-13 独立行政法人科学技術振興機構 チロシルｔＲＮＡ合成酵素変異体
CN1379094A (zh) * 2002-02-01 2002-11-13 杭州华大基因研发中心 耐高温酪氨酰tRNA合成酶基因及其编码的多肽和制备方法
CA2500653A1 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
US7432092B2 (en) * 2002-10-16 2008-10-07 The Scripps Research Institute Site specific incorporation of keto amino acids into proteins
EP1583816A4 (de) * 2002-12-22 2007-06-13 Scripps Research Inst Proteinarrays
KR101171397B1 (ko) 2003-04-17 2012-08-07 더 스크립스 리서치 인스티튜트 진핵 유전자 코드의 확장
CA2527877A1 (en) * 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
EP1658366A4 (de) 2003-07-07 2006-08-09 Scripps Research Inst Zusammensetzungen von orthogonalen glutamyl-trna- und aminoacyl-trna-synthetasepaaren und deren verwendungen
ES2301099T3 (es) 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
DK1687401T3 (da) 2003-10-14 2013-02-04 Scripps Research Inst Sted-spicifik inkorporing af redoxaktive aminosyrer i protiener
EP1725251A4 (de) 2003-12-18 2009-07-01 Scripps Research Inst Selektive einarbeitung von 5-hydroxytryptophan in proteine in säugetierzellen
MXPA06013586A (es) * 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
ES2666693T3 (es) 2004-09-21 2018-05-07 The Scripps Research Institute Incorporación in vivo de alquinil aminoácidos a proteínas en eubacterias
US20090181858A1 (en) 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
WO2006036779A2 (en) 2004-09-22 2006-04-06 The Scripps Research Institute Site-specific labeling of proteins for nmr studies
WO2006045116A2 (en) 2004-10-20 2006-04-27 The Scripps Research Institute In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
WO2006110182A2 (en) 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US8367588B2 (en) 2005-10-12 2013-02-05 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
ES2432391T3 (es) 2006-03-09 2013-12-03 The Scripps Research Institute Sistema para la expresión de componentes de traducción ortogonales en células huésped eubacterianas
BRPI0709603A2 (pt) 2006-03-16 2011-07-19 Scripps Research Inst expressão de proteìnas geneticamente programada contendo o aminoácido não natural fenilselenocisteìna
CA2652894A1 (en) 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
EP2052083B1 (de) 2006-09-21 2014-01-08 The Scripps Research Institute Genetisch programmierte expression von selektiv sulfatierten proteinen in eubakterien
EP2069481A4 (de) 2006-10-18 2010-01-06 Scripps Research Inst Genetischer einbau unnatürlicher aminosäuren in proteine in säugerzellen
CA2703736A1 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
US20090148887A1 (en) 2007-11-02 2009-06-11 The Scripps Research Institute Genetically encoded boronate amino acid
CA2704494A1 (en) 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
WO2009064416A2 (en) 2007-11-15 2009-05-22 The Scripps Research Institute Genetic incorporation of an alpha-hydroxy acid into proteins to generate ester backbone linkages at defined sites
JP2011514322A (ja) 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
WO2009151491A2 (en) 2008-02-27 2009-12-17 The Scripps Research Institute In vivo incorporation of an unnatural amino acid comprising a 1,2-aminothiol group
US20090197339A1 (en) 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
JP5959201B2 (ja) 2008-12-10 2016-08-02 ザ スクリプス リサーチ インスティテュート 化学的に反応性の非天然アミノ酸を使用するキャリア−ペプチド接合体の製造
WO2010114615A2 (en) 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells

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