ATE544747T1 - Erkennung von transmembranpotentialen durch optische verfahren - Google Patents

Erkennung von transmembranpotentialen durch optische verfahren

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Publication number
ATE544747T1
ATE544747T1 AT00984287T AT00984287T ATE544747T1 AT E544747 T1 ATE544747 T1 AT E544747T1 AT 00984287 T AT00984287 T AT 00984287T AT 00984287 T AT00984287 T AT 00984287T AT E544747 T1 ATE544747 T1 AT E544747T1
Authority
AT
Austria
Prior art keywords
reagent
membrane
cell
changes
potential
Prior art date
Application number
AT00984287T
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English (en)
Inventor
Roger Tsien
Jesus Gonzalez
Original Assignee
Univ California
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Publication of ATE544747T1 publication Critical patent/ATE544747T1/de

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    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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AT00984287T 1999-12-13 2000-12-12 Erkennung von transmembranpotentialen durch optische verfahren ATE544747T1 (de)

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Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US6593135B2 (en) * 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7338782B2 (en) 1998-12-11 2008-03-04 Clontech Laboratories, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
US20030087492A1 (en) 2001-11-02 2003-05-08 Promos Technologies, Inc. Semiconductor device and method of manufacturing the same
US9834584B2 (en) * 1998-12-11 2017-12-05 Takara Bio Usa, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
US6537771B1 (en) 1999-10-08 2003-03-25 Caliper Technologies Corp. Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
CA2383642C (en) 1999-10-14 2014-03-25 Clontech Laboratories, Inc. Anthozoa derived chromophores/fluorophores and methods for using the same
GB0012229D0 (en) * 2000-05-19 2000-07-12 Devgen Nv Lipid uptake assays
JP4330338B2 (ja) * 2000-10-12 2009-09-16 クローンテック ラボラトリーズ インク． 大きく赤側にシフトした蛍光タンパク質
US20020183271A1 (en) * 2000-12-07 2002-12-05 Sunil Chada Methods of treatment involving human MDA-7
US6969597B2 (en) * 2001-02-21 2005-11-29 Clontech Laboratories, Inc. Nucleic acids encoding non aggregating fluorescent proteins and methods for using the same
US7015310B2 (en) 2001-03-12 2006-03-21 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation reduction sensitive green fluorescent protein variants
US20030194798A1 (en) * 2001-05-24 2003-10-16 Surber Mark W. Minicell compositions and methods
CA2448719A1 (en) * 2001-06-09 2002-12-19 Glsynthesis Inc. Lipid structures and uses thereof
EP1409982A4 (de) * 2001-06-14 2006-05-24 Anadys Pharmaceuticals Inc Verfahren zum screening auf liganden von zielmolekülen
WO2003006606A2 (en) * 2001-07-09 2003-01-23 Oklahoma Medical Research Foundation Targeted fusion proteins and methods for the characterization of cellular membrane domains
US6852504B2 (en) * 2001-08-08 2005-02-08 Molecular Devices Corporation Method for measuring cellular transmembrane potential changes
US20030082511A1 (en) * 2001-09-25 2003-05-01 Brown Steven J. Identification of modulatory molecules using inducible promoters
TW200406466A (en) * 2001-11-13 2004-05-01 Ciba Sc Holding Ag Compositions comprising at least one oxonol dye and at least one metal complex
JP2005533000A (ja) * 2002-03-05 2005-11-04 ボード　オブ　リージェンツ，　ザ　ユニバーシティ　オブ　テキサス　システム Ｍｄａ−７に関与する免疫誘導を増強する方法
US20030228703A1 (en) * 2002-04-05 2003-12-11 The Regents Of The University Of Michigan Fluorescence resonance energy transfer quantitation and stoichiometry in living cells
US20030228566A1 (en) * 2002-06-11 2003-12-11 Biotechplex Corporation Method of and apparatus for screening for drug candidates
JP4429577B2 (ja) * 2002-09-30 2010-03-10 株式会社ニコン 細胞内反応測定装置および細胞内反応測定方法
US7432036B2 (en) * 2002-10-07 2008-10-07 Fujifilm Corporation Non-resonant two-photon absorbing material, non-resonant two-photon emitting material, and method for inducing absorption or generating emission of non-resonant two photons by using the material
AU2003285844A1 (en) * 2002-11-12 2004-06-03 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluorescent proteins and chromoproteins from non-aequorea hydrozoa species and methods for using same
US7413874B2 (en) * 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
EP1603943A2 (de) * 2003-03-03 2005-12-14 Board of Regents, The University of Texas System Verfahren und zusammensetzungen mit mda-7
WO2004087884A2 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc TARGETING ENZYMES OF THE tRNA SPLICING PATHWAY FOR IDENTIFICATION OF ANTI-FUNGAL AND/OR ANTI-PROLIFERATIVE MOLECULES
WO2004087069A2 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. METHODS OF IDENTIFYING COMPOUNDS THAT TARGET tRNA SPLICING ENDONUCLEASE AND USES OF SAID COMPOUNDS AS ANTI-PROLIFERATIVE AGENTS
WO2004094597A2 (en) * 2003-04-16 2004-11-04 University Of Miami Anthozoan fluorescent protein genes
JP2004357552A (ja) * 2003-06-03 2004-12-24 Hitachi Ltd 作用物質による効果推定方法及びスクリーニング方法
FR2858177A1 (fr) * 2003-07-28 2005-02-04 Genethon Utilisation du phenomeme fret, detecte par mplsm, pour le suivi in vivo d'evenements biologiques
US20050037364A1 (en) * 2003-08-13 2005-02-17 International Business Machines Corporation Techniques for recording signals
US7125908B2 (en) * 2003-08-29 2006-10-24 Allergan, Inc. Treating pain using selective antagonists of persistent sodium current
US7060723B2 (en) 2003-08-29 2006-06-13 Allergan, Inc. Treating neurological disorders using selective antagonists of persistent sodium current
CA2548220A1 (en) * 2003-12-01 2005-09-09 Introgen Therapeutics, Inc. Use of mda-7 to inhibit infection by pathogenic organisms
GB0403616D0 (en) * 2004-02-18 2004-03-24 Univ Cambridge Tech Derivatised phospholipids
US20070281041A1 (en) * 2004-03-02 2007-12-06 Introgen Therapeutics, Inc. Compositions and Methods Involving MDA-7 for the Treatment of Cancer
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
GB0414653D0 (en) * 2004-06-30 2004-08-04 Univ Edinburgh Membrane fusion assay
US20060275310A1 (en) * 2004-09-30 2006-12-07 Nano Science Diagnostics, Inc. Method and detection and decontamination of antigens by nanoparticle-raman spectroscopy
TWI364882B (en) 2004-10-15 2012-05-21 Tyco Electronics Corp Connector system for conductive plates
CN101166539B (zh) * 2005-02-08 2012-01-04 得克萨斯大学体系董事会 用于治疗癌症的涉及mda-7的组合物和方法
US20080281104A1 (en) * 2005-03-04 2008-11-13 The University Of Tokyo Membrane-Anchoring Fluorescent Probe
US10052497B2 (en) 2005-07-22 2018-08-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US9238150B2 (en) 2005-07-22 2016-01-19 The Board Of Trustees Of The Leland Stanford Junior University Optical tissue interface method and apparatus for stimulating cells
EP1919497B1 (de) 2005-07-22 2020-02-12 The Board of Trustees of the Leland Stanford Junior University Lichtaktivierter kationenkanal und dessen verwendung
US9274099B2 (en) * 2005-07-22 2016-03-01 The Board Of Trustees Of The Leland Stanford Junior University Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
US8926959B2 (en) * 2005-07-22 2015-01-06 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
ATE555387T1 (de) * 2005-10-12 2012-05-15 Allergan Inc Tests der molekularen oder subzellulären nähe unter verwendung von depolarisierung nach resonanzenergietransfer (daret)
EP1803780B1 (de) * 2005-12-20 2009-03-25 Agfa Graphics N.V. Kontrastfarbstoffe für Tintenstrahllithographiedruckplatten
US8718758B2 (en) * 2006-06-19 2014-05-06 Highland Instruments, Inc. Interface apparatus for stimulation of biological tissue
US8892200B2 (en) 2006-06-19 2014-11-18 Highland Instruments, Inc. Systems and methods for stimulating tissue using focused energy
JP5250549B2 (ja) 2006-06-19 2013-07-31 ハイランド インストゥルメンツ， インコーポレイテッド 生体組織の刺激のための器具および方法
US9913976B2 (en) 2006-06-19 2018-03-13 Highland Instruments, Inc. Systems and methods for stimulating and monitoring biological tissue
US8155730B2 (en) * 2006-10-24 2012-04-10 The Research Foundation Of State University Of New York Composition, method, system, and kit for optical electrophysiology
WO2008086470A1 (en) 2007-01-10 2008-07-17 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
WO2008101128A1 (en) 2007-02-14 2008-08-21 The Board Of Trustees Of The Leland Stanford Junior University System, method and applications involving identification of biological circuits such as neurological characteristics
WO2008106694A2 (en) 2007-03-01 2008-09-04 The Board Of Trustees Of The Leland Stanford Junior University Systems, methods and compositions for optical stimulation of target cells
US7994786B2 (en) * 2007-06-19 2011-08-09 Mary Hitchcock Memorial Hospital System and method for use of nanoparticles in imaging and temperature measurement
CA2695216A1 (en) * 2007-07-31 2009-02-05 Purdue Pharma L.P. Dielectric spectroscopy assays for screening of ion channel ligands
JP4604187B2 (ja) * 2007-08-14 2010-12-22 独立行政法人産業技術総合研究所 糖鎖を介したルシフェラーゼと有機蛍光色素間のエネルギー移動を利用した可視−近赤外光プローブ
US10035027B2 (en) 2007-10-31 2018-07-31 The Board Of Trustees Of The Leland Stanford Junior University Device and method for ultrasonic neuromodulation via stereotactic frame based technique
US10434327B2 (en) 2007-10-31 2019-10-08 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
EP2062944A1 (de) * 2007-11-20 2009-05-27 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Wasserlösliche Rylen-Farbstoffe, Verfahren zu ihrer Herstellung und ihre Verwendung als Fluoreszenzmarker für Biomoleküle
MY169771A (en) 2008-04-23 2019-05-15 Univ Leland Stanford Junior Systems, methods and compositions for optical stimulation of target cells
WO2009148946A2 (en) 2008-05-29 2009-12-10 The Board Of Trustees Of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
JP2011525130A (ja) 2008-06-17 2011-09-15 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 光伝送素子を用いた標的細胞の光刺激のための方法、システム、および装置
EP2303405A4 (de) 2008-06-17 2017-12-27 The Board of Trustees of the Leland Stanford Junior University Gerät und verfahren zur kontrolle der zellentwicklung
WO2010006049A1 (en) 2008-07-08 2010-01-14 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
US20100029017A1 (en) * 2008-07-29 2010-02-04 Becton, Dickinson And Company Mono-chlorinated hydroxycoumarin conjugates
NZ602416A (en) 2008-11-14 2014-08-29 Univ Leland Stanford Junior Optically-based stimulation of target cells and modifications thereto
US8431416B2 (en) * 2009-04-01 2013-04-30 Becton, Dickinson And Company Reactive heterocycle-substituted 7-hydroxycoumarins and their conjugates
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
WO2011116238A2 (en) 2010-03-17 2011-09-22 The Board Of Trustees Of The Leland Stanford Junior University. Light-sensitive ion-passing molecules
DE102010012857B4 (de) * 2010-03-25 2015-02-19 Tim Liedl Vorrichtung zum Erfassen einer Weggröße
US9681820B2 (en) 2010-10-21 2017-06-20 Highland Instruments, Inc. Systems for detecting a condition
CN106376525A (zh) 2010-11-05 2017-02-08 斯坦福大学托管董事会 记忆功能的控制和表征
CN106106368A (zh) 2010-11-05 2016-11-16 斯坦福大学托管董事会 光控cns功能障碍
ES2684307T3 (es) 2010-11-05 2018-10-02 The Board Of Trustees Of The Leland Stanford Junior University Proteínas estabilizadas de tipo opsina de función escalonada y métodos de uso de las mismas
WO2012061679A2 (en) 2010-11-05 2012-05-10 The Board Of Trustees Of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
AU2011323235B2 (en) 2010-11-05 2015-10-29 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of reward-related behaviors
CN106422081B (zh) 2010-11-05 2019-06-21 斯坦福大学托管董事会 用于光遗传学方法的光的上转换
US8696722B2 (en) 2010-11-22 2014-04-15 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US8594783B2 (en) 2011-08-24 2013-11-26 Highland Instruments, Inc. Systems and methods for stimulating cellular function in tissue
CA2859364C (en) 2011-12-16 2019-05-07 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
EP3205374B1 (de) 2012-02-21 2019-03-27 The Board of Trustees of The Leland Stanford Junior University Zusammensetzungen zur behandlung neurogener erkrankungen des beckenbodens
WO2013130808A1 (en) 2012-02-29 2013-09-06 D.E. Shaw Research, Llc Methods for screening voltage gated proteins
KR20150095755A (ko) * 2012-12-11 2015-08-21 클래리언트 다이아그노스틱 서비시즈, 인크. 염료의 광활성화 화학적 표백
US9636380B2 (en) 2013-03-15 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of inputs to the ventral tegmental area
JP6594854B2 (ja) 2013-03-15 2019-10-23 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 行動状態の光遺伝学的制御方法
AU2014260101B2 (en) 2013-04-29 2018-07-26 Humboldt-Universitat Zu Berlin Devices, systems and methods for optogenetic modulation of action potentials in target cells
US8933239B1 (en) 2013-07-16 2015-01-13 Dow Global Technologies Llc Bis(aryl)acetal compounds
US8962779B2 (en) 2013-07-16 2015-02-24 Dow Global Technologies Llc Method of forming polyaryl polymers
US9063420B2 (en) 2013-07-16 2015-06-23 Rohm And Haas Electronic Materials Llc Photoresist composition, coated substrate, and method of forming electronic device
US9410016B2 (en) 2013-07-16 2016-08-09 Dow Global Technologies Llc Aromatic polyacetals and articles comprising them
EP3033427A4 (de) 2013-08-14 2017-05-31 The Board Of Trustees Of The University Of the Leland Stanford Junior University Zusammensetzungen und verfahren zur bekämpfung von schmerzen
CN104101589B (zh) * 2014-07-29 2016-04-27 重庆大学 基于tmrm的细胞静息膜电位和钠钾离子通透性比值检测方法
USD759803S1 (en) 2014-10-28 2016-06-21 Highland Instruments, Inc. Adjustable headpiece with anatomical markers
WO2016205027A1 (en) * 2015-06-15 2016-12-22 Oklahoma Medical Research Foundation Ubiquitin interacting motif peptides as therapeutics
WO2016209654A1 (en) 2015-06-22 2016-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging and/or optogenetic control of light-responsive neurons
US10705092B2 (en) 2017-01-30 2020-07-07 The Government of the United States of Americam as Represented by the Secretary of the Navy Modular, multifunctional nanoparticle-based bioconjugate for realtime visualization of cellular membrane potential
US11294165B2 (en) 2017-03-30 2022-04-05 The Board Of Trustees Of The Leland Stanford Junior University Modular, electro-optical device for increasing the imaging field of view using time-sequential capture
CN107118127B (zh) * 2017-06-28 2019-01-04 哈尔滨理工大学 氨基酸席夫碱及其合成方法与应用
AU2019368519B2 (en) * 2018-10-26 2022-11-03 Shandong University System and method for real-time screening and measurement of cellular specific photosensitive effect
US20220113313A1 (en) * 2019-02-01 2022-04-14 NanoView Biosciences, Inc. Systems and methods for vesicle cargo labeling and detection
US20220205914A1 (en) * 2020-12-31 2022-06-30 Q-State Biosciences, Inc. Plate imager
CN115420779B (zh) * 2022-09-14 2025-06-03 西南大学 Pt NPs@TPB NCs/溶解O2三元电致化学发光生物传感器、制备方法及应用
CN116272897B (zh) * 2023-02-27 2024-08-27 中国检验检疫科学研究院 富集香豆素类化合物的磁性纳米粒及其制备方法和应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE43619C (de) * 1887-06-25 1888-06-23 C. RAAB in Kaiserslautern Calorimetrischer Elektricitätszähler
US3480436A (en) * 1966-08-29 1969-11-25 Eastman Kodak Co Antihalation compositions containing pentamethine oxonol dyes in aromatic alcohols
JPS59218956A (ja) 1983-05-27 1984-12-10 Fujirebio Inc 膜融合測定方法
ES8607563A1 (es) 1983-10-13 1986-06-01 Univ Georgia Res Found Un metodo para detectar una interaccion ligante
US4861727A (en) 1986-09-08 1989-08-29 C. R. Bard, Inc. Luminescent oxygen sensor based on a lanthanide complex
US4900934A (en) 1987-07-15 1990-02-13 University Of Utah Apparatus for simultaneous visualization and measurement of fluorescence from fluorescent dye-treated cell preparations and solutions
WO1990006508A1 (en) * 1988-11-25 1990-06-14 Benner Richard L Process and apparatus for the detection of sulfur
AT393035B (de) 1989-04-25 1991-07-25 Avl Verbrennungskraft Messtech Verfahren zur quantitativen bestimmung zumindest eines chemischen parameters eines probenmediums
ATE106566T1 (de) 1989-11-21 1994-06-15 Bayer Ag Optischer biosensor.
US5244813A (en) 1991-01-25 1993-09-14 Trustees Of Tufts College Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample
DE4121426A1 (de) 1991-06-28 1993-01-14 Basf Ag Chemischer sensor
EP0552107A1 (de) 1992-01-17 1993-07-21 The University Of Maryland At Baltimore pH und pC02-Messung durch Luminiszenz-Lebensdauer und Energietransfer
CA2129105A1 (en) 1992-02-12 1993-08-19 Michael J. Conrad Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
WO1995008637A1 (en) 1993-09-21 1995-03-30 Washington State University Research Foundation Immunoassay comprising ligand-conjugated, ion channel receptor immobilized in lipid film
GB9406464D0 (en) 1994-03-31 1994-05-25 British Tech Group Assay for determining biochemical changes at phospholipid bilayer membrane surfaces
US5661035A (en) * 1995-06-07 1997-08-26 The Regents Of The University Of California Voltage sensing by fluorescence resonance energy transfer
AU5090498A (en) 1997-01-07 1998-08-03 California Institute Of Technology Optical sensors of cell signaling

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