ATE547532T1 - Verfahren zur erzeugung von hochgradig diversen bibliotheken - Google Patents

Verfahren zur erzeugung von hochgradig diversen bibliotheken

Info

Publication number
ATE547532T1
ATE547532T1 AT99932081T AT99932081T ATE547532T1 AT E547532 T1 ATE547532 T1 AT E547532T1 AT 99932081 T AT99932081 T AT 99932081T AT 99932081 T AT99932081 T AT 99932081T AT E547532 T1 ATE547532 T1 AT E547532T1
Authority
AT
Austria
Prior art keywords
nucleic acid
library
fragments
templates
strand
Prior art date
Application number
AT99932081T
Other languages
English (en)
Inventor
Richard Wagner
Martin Wright
Brent Kreider
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Application granted granted Critical
Publication of ATE547532T1 publication Critical patent/ATE547532T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT99932081T 1998-06-29 1999-06-29 Verfahren zur erzeugung von hochgradig diversen bibliotheken ATE547532T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9097098P 1998-06-29 1998-06-29
PCT/US1999/014776 WO2000000632A1 (en) 1998-06-29 1999-06-29 Methods for generating highly diverse libraries

Publications (1)

Publication Number Publication Date
ATE547532T1 true ATE547532T1 (de) 2012-03-15

Family

ID=22225171

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99932081T ATE547532T1 (de) 1998-06-29 1999-06-29 Verfahren zur erzeugung von hochgradig diversen bibliotheken

Country Status (13)

Country Link
EP (1) EP1092039B1 (de)
JP (1) JP4700805B2 (de)
KR (1) KR20010071613A (de)
CN (1) CN1308683A (de)
AT (1) ATE547532T1 (de)
AU (1) AU761570B2 (de)
CA (1) CA2331926C (de)
ES (1) ES2381824T3 (de)
IL (1) IL140100A0 (de)
NO (1) NO20006675D0 (de)
NZ (1) NZ508287A (de)
WO (1) WO2000000632A1 (de)
ZA (1) ZA200007261B (de)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
KR100618495B1 (ko) 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US7488590B2 (en) 1998-10-23 2009-02-10 Amgen Inc. Modified peptides as therapeutic agents
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
ES2341217T3 (es) 1999-01-19 2010-06-17 Maxygen, Inc. Recombinacion de acidos nucleicos mediada por oligonucleotidos.
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
AU1456101A (en) 1999-11-03 2001-05-14 Maxygen, Inc. Antibody diversity generation
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
US6980674B2 (en) 2000-09-01 2005-12-27 Large Scale Proteomics Corp. Reference database
US6539102B1 (en) 2000-09-01 2003-03-25 Large Scale Proteomics Reference database
WO2005012515A2 (en) 2003-04-29 2005-02-10 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
US6783941B2 (en) 2000-12-06 2004-08-31 Novozymes A/S Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes
AU2002220529A1 (en) * 2000-12-06 2002-06-18 Novozymes A/S Method for producing a polynucleotide library
US6689568B2 (en) 2001-02-01 2004-02-10 Agilent Technologies, Inc. Capture arrays using polypeptide capture agents
CA2436214C (en) * 2001-02-02 2012-12-04 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
AU2002307340A1 (en) 2001-04-16 2002-10-28 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
US20030032028A1 (en) * 2001-06-12 2003-02-13 Gayle Dace In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
WO2003062417A1 (fr) * 2002-01-22 2003-07-31 Mitsubishi Chemical Corporation Produit de ligation arn-adn, et utilisation correspondante
JP5319865B2 (ja) 2002-03-01 2013-10-16 コデクシス メイフラワー ホールディングス, エルエルシー 機能的生体分子を同定する方法、システム、およびソフトウェア
EP1488335A4 (de) 2002-03-09 2006-11-15 Maxygen Inc Optimierung von crossover-punkten für die gerichtete evolution
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
PT1497418E (pt) 2002-04-19 2013-01-25 Dsm Ip Assets Bv Fosfolípases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
BR0313281A (pt) 2002-08-06 2007-07-24 Verdia Inc variantes de ap1 amina oxidase
CN102618564B (zh) 2003-03-06 2014-10-29 维莱尼姆公司 淀粉酶、编码它们的核酸及其制备和应用方法
CA3007908A1 (en) 2003-03-07 2005-04-14 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
HUE030493T2 (en) 2003-04-04 2017-05-29 Basf Enzymes Llc Pectate lyases, nucleic acids and processes encoding them for their production and use
EP1639091B1 (de) 2003-06-17 2012-12-05 California University Of Technology Regio- und enantioselektive Alkanhydroxylierung mit modifiziertem Cytochrom p450
US7960148B2 (en) 2003-07-02 2011-06-14 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
CA2535526C (en) 2003-08-11 2015-09-29 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
US7435570B2 (en) 2003-08-11 2008-10-14 California Institute Of Technology Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use
JP2005065575A (ja) * 2003-08-22 2005-03-17 Japan Science & Technology Agency フレームシャッフリングによるタンパク質分子多様性集団の作製
AU2005264938B2 (en) 2004-06-16 2011-11-24 Verenium Corporation Compositions and methods for enzymatic decolorization of chlorophyll
EP2886658A1 (de) 2005-03-10 2015-06-24 BASF Enzymes LLC Lyaseenzyme, Nukleinsäuren zur Codierung davon und Verfahren zur Herstellung und Verwendung davon
CA2611859C (en) 2005-03-15 2015-03-31 Verenium Corporation Cellulases, nucleic acids encoding them and methods for making and using them
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
EP1987142A4 (de) 2006-02-02 2009-07-15 Verenium Corp Esterasen und damit zusammenhängende nukleinsäuren und verfahren
NZ595497A (en) 2006-02-10 2013-09-27 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
TR201809912T4 (tr) 2006-02-14 2018-07-23 Bp Corp North America Inc Ksilanazlar, bunları kodlayan nükleik asitler ve bunları yapmak ve kullanmak için yöntemler.
AU2007223086B2 (en) 2006-03-07 2013-09-26 Basf Enzymes Llc Aldolases, nucleic acids encoding them and methods for making and using them
MX2008011477A (es) 2006-03-07 2008-09-23 Cargill Inc Aldolasas, acidos nucleicos que las codifican y metodos para hacerlas y usarlas.
DK2069389T3 (en) 2006-08-04 2015-01-12 Bp Corp North America Inc Glucanases, nucleic acids encoding them, and processes for their preparation and use
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
EP2057274B1 (de) 2006-09-21 2013-12-11 DSM IP Assets B.V. Phospholipase ,nukleinsaüre, die diese kodieren und verfahren zur ihrer herstellung und verwendung
ES2531135T3 (es) 2006-09-21 2015-03-11 Basf Enzymes Llc Fitasas, ácidos nucleicos que las codifican y métodos para su producción y uso
EP2505651A3 (de) 2006-12-10 2013-01-09 Dyadic International, Inc. Pilzisolate mit reduzierter Proteaseaktivität
PL2479267T3 (pl) 2006-12-21 2017-06-30 Basf Enzymes Llc Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
NZ598285A (en) 2007-01-30 2013-10-25 Syngenta Participations Ag Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
BRPI0817811A2 (pt) 2007-10-03 2014-10-14 Verenium Corp Xilanases, ácidos nucleicos que codificam as mesmas e métodos para fabricação e uso das mesmas.
WO2009088753A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
CN104651381A (zh) 2008-01-03 2015-05-27 巴斯夫酶有限责任公司 转移酶和氧化还原酶、编码它们的核酸以及其制备和应用方法
WO2009143397A2 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
CN102227503B (zh) 2008-09-26 2015-10-21 托卡根公司 重组载体
NZ596459A (en) 2009-05-21 2013-11-29 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
BR112013003135A2 (pt) 2010-08-13 2017-11-07 Pioneer Hi Bred Int polinucletídeo e polipeptídeo isolado ou recombinante, construto de ácido nucleico, célula, planta, explante vegetal, semente transgênica, método para produção de célula vegetal para controle de plantas daninhas e para detecção de um polipeptídeo hppd e um polinucleotídeo.
WO2013016207A2 (en) 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
US20150184173A1 (en) 2012-05-04 2015-07-02 E I Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
JP6004423B2 (ja) * 2012-05-07 2016-10-05 国立研究開発法人産業技術総合研究所 遺伝子連結法およびそれを用いた単鎖抗体作製方法
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
BR112015023272A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int célula vegetal, planta, explante vegetal, semente transgênica, método para produzir uma célula vegetal tendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade de dicamba descarboxilase, método para controlar plantas daninhas em um campo contendo uma cultura e método para controlar plantas daninhas em um campo contendo uma cultura
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
BR112016003225B1 (pt) 2013-08-16 2022-10-25 Pioneer Hi-Bred International, Inc. Polipeptídeo pip-47, polipeptídeo pip-47 quimérico, composição, proteína de fusão, método para controlar uma população de inseto-praga, método para inibir o crescimento ou matar um inseto-praga, construto de dna, polinucleotídeo isolado, cassete de expressão, método de obtenção de uma planta transgênica e método para controlar infestação de inseto
BR122020001770B1 (pt) 2013-09-13 2022-11-29 Pioneer Hi-Bred International, Inc Construto de dna, método de obtenção de planta transgênica, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto
EP4122945A1 (de) 2013-12-23 2023-01-25 University of Rochester Verfahren und zusammensetzungen zur ribosomalen synthese von makrocyclischen peptiden
BR112016018103B1 (pt) 2014-02-07 2024-01-16 E.I. Du Pont De Nemours And Company Polipeptídeo e seu uso, polinucleotídeo, composição, proteína de fusão, método para controlar uma população, método para inibir o crescimento, método para controlar a infestação, método para obtenção de uma planta ou célula vegetal, construto
CN106536545B (zh) 2014-02-07 2026-03-03 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN113372421B (zh) 2014-10-16 2024-08-06 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN114075267B (zh) 2015-01-15 2025-03-18 先锋国际良种公司 杀昆虫蛋白及其使用方法
US10968536B2 (en) 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
CA2977026A1 (en) 2015-03-11 2016-09-15 E.I. Du Pont De Nemours And Company Insecticidal combinations of pip-72 and methods of use
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112018002535A2 (pt) 2015-08-06 2018-09-25 Du Pont polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, planta transgênica ou célula de planta, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas ou uma praga e uso do polipeptídeo
CN108575091A (zh) 2015-12-18 2018-09-25 先锋国际良种公司 杀昆虫蛋白及其使用方法
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
CA3018384A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3478052B1 (de) 2016-07-01 2021-08-25 Pioneer Hi-Bred International, Inc. Insektizidproteine aus pflanzen und verfahren zu deren verwendung
EP3535285B1 (de) 2016-11-01 2022-04-06 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
BR112019012339A2 (pt) 2016-12-14 2019-11-26 Pioneer Hi Bred Int polipeptídeo inseticida recombinante, composição, construto de dna, célula hospedeira, planta transgênica, método para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, polipeptídeo ipd093 quimérico e proteína de fusão
US11213028B2 (en) 2016-12-22 2022-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018148001A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International Inc Insecticidal combinations of plant derived insecticidal proteins and methods for their use
RU2019140646A (ru) 2017-05-11 2021-06-11 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CN110158157B (zh) * 2018-02-13 2021-02-02 浙江大学 基于模板材料合成长度固定和特定末端序列dna文库的方法
AU2019234562B2 (en) 2018-03-14 2024-08-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CA3092078A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US20220169706A1 (en) 2019-03-28 2022-06-02 Danisco Us Inc Engineered antibodies
WO2021142393A1 (en) 2020-01-10 2021-07-15 The Regents Of The University Of California Biosynthetic platform for the production of olivetolic avid and analogues of olivetolic acid
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) * 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US5447839A (en) * 1988-09-09 1995-09-05 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5580730A (en) * 1994-08-19 1996-12-03 Olympus America, Inc. Enzyme digestion method for the detection of amplified DNA
WO1997021837A1 (en) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Methods for the detection and removal of mutant sequences that arise during enzymatic amplification using mismatch repair systems
WO1998041653A1 (en) * 1997-03-18 1998-09-24 Novo Nordisk A/S An in vitro method for construction of a dna library
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers

Also Published As

Publication number Publication date
ES2381824T3 (es) 2012-05-31
EP1092039A1 (de) 2001-04-18
WO2000000632A9 (en) 2000-10-26
NO20006675L (no) 2000-12-28
AU4847099A (en) 2000-01-17
AU761570B2 (en) 2003-06-05
WO2000000632A1 (en) 2000-01-06
CN1308683A (zh) 2001-08-15
CA2331926C (en) 2013-09-10
JP4700805B2 (ja) 2011-06-15
IL140100A0 (en) 2002-02-10
CA2331926A1 (en) 2000-01-06
NZ508287A (en) 2003-08-29
NO20006675D0 (no) 2000-12-28
ZA200007261B (en) 2002-05-15
JP2002519038A (ja) 2002-07-02
KR20010071613A (ko) 2001-07-28
EP1092039B1 (de) 2012-02-29
EP1092039A4 (de) 2003-07-02

Similar Documents

Publication Publication Date Title
ATE547532T1 (de) Verfahren zur erzeugung von hochgradig diversen bibliotheken
AU672969B2 (en) Exchangeable template reaction
JP2002519038A5 (de)
Cukras et al. Chess games: a model for RNA based computation
DE69900592D1 (de) Verfahren zur unspezifischen amplifizierung einer nukleinsäure
EP1963525B1 (de) Synthese von nukleinsäuremolekülen mit minimierter fehlerzahl
DK0868530T3 (da) Kaskade-nucleinsyreamplificeringsreaktion
ATE323179T1 (de) Verfahren zur synthese von dna-fragmenten
DE3687537D1 (de) Verfahren zur amplifikation, zum nachweis und/oder zur klonierung von nukleinsaeuresequenzen.
DE3852177D1 (de) Transkriptionsbasierte nukleinsäure-verstärkungs/nachweissysteme.
ES2133709T3 (es) Transcripcion de adn bicatenario inducida por analogo de acido nucleico.
DE69016826D1 (de) Verfahren zur Synthese von Ribonukleinsäure (RNS).
ATE431407T1 (de) Verfahren zur herstellung von dna
WO2003072798A3 (en) Method for generating amplified rna
ATE307901T1 (de) Amplifikation und sequenzierung von primer-paaren und deren verwendung
ATE319861T1 (de) Verbesserte methoden zur erzeugung einer vielzahl von rna-kopien
CA2264967A1 (en) Method of dna sequencing
EP1144689A1 (de) Dna sequenzierungsmethode die verschiedene dna polymerasen verwendet und entsprechender kit
US12297490B2 (en) Methods for asymmetric DNA library generation and optionally integrated duplex sequencing
ATE223499T1 (de) Von kreisförmiger matrize abhängige kettenreaktion
Green et al. Conserved sequence and functional domains in satellite 2 from three families of salamanders.
Harrison et al. Easy gene walking
JP2025510151A5 (de)
WO2002050094A3 (en) Nucleic acid synthesis by primer extension on successive templates
WO2001038573A1 (en) Dna sequencing method which employs various nucleotide mixtures and kit used for the same