ATE551349T1 - Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden - Google Patents
Methode unter verwendung von reversibel blockierten markierungsoligonukleotidenInfo
- Publication number
- ATE551349T1 ATE551349T1 AT05814417T AT05814417T ATE551349T1 AT E551349 T1 ATE551349 T1 AT E551349T1 AT 05814417 T AT05814417 T AT 05814417T AT 05814417 T AT05814417 T AT 05814417T AT E551349 T1 ATE551349 T1 AT E551349T1
- Authority
- AT
- Austria
- Prior art keywords
- reversibly blocked
- labeling oligonucleotides
- dna molecule
- blocked labeling
- tag
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 238000002372 labelling Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 230000004544 DNA amplification Effects 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CA2005/001830 WO2007062495A1 (en) | 2005-11-30 | 2005-11-30 | Selective terminal tagging of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE551349T1 true ATE551349T1 (de) | 2012-04-15 |
Family
ID=38091822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05814417T ATE551349T1 (de) | 2005-11-30 | 2005-11-30 | Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8304183B2 (de) |
| EP (1) | EP1954706B1 (de) |
| AT (1) | ATE551349T1 (de) |
| AU (1) | AU2005338632B2 (de) |
| CA (1) | CA2639819C (de) |
| WO (1) | WO2007062495A1 (de) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2173872B1 (de) * | 2007-06-29 | 2014-04-02 | CellScript, Inc. | Copy-dna und sense-rna |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP2644710B1 (de) * | 2008-04-30 | 2016-12-21 | Integrated Dna Technologies, Inc. | Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren |
| US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| WO2011045744A2 (en) * | 2009-10-13 | 2011-04-21 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
| US20110117559A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| WO2011100569A1 (en) * | 2010-02-12 | 2011-08-18 | Genisphere, Llc | Whole transcriptome sequencing |
| CA2807552A1 (en) | 2010-08-06 | 2012-02-09 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| ES3005233T3 (en) | 2010-10-01 | 2025-03-14 | Modernatx Inc | Modified nucleosides, nucleotides, and nucleic acids, and uses thereof |
| AU2012236896A1 (en) | 2011-03-25 | 2013-05-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| JP2014511687A (ja) | 2011-03-31 | 2014-05-19 | モデルナ セラピューティクス インコーポレイテッド | 工学操作された核酸の送達および製剤 |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| EP2570487A1 (de) | 2011-09-16 | 2013-03-20 | Lexogen GmbH | Verfahren zur Nukleinsäuretranskription |
| PL2756098T3 (pl) | 2011-09-16 | 2018-12-31 | Lexogen Gmbh | Sposób wytwarzania biblioteki cząsteczek kwasów nukleinowych |
| EP2763701B1 (de) | 2011-10-03 | 2018-12-19 | Moderna Therapeutics, Inc. | Modifizierte nukleoside, nukleotide und nukleinsäuren und verwendungen davon |
| JP2015501844A (ja) | 2011-12-16 | 2015-01-19 | モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | 修飾ヌクレオシド、ヌクレオチドおよび核酸組成物 |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| US9254311B2 (en) | 2012-04-02 | 2016-02-09 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
| WO2013151663A1 (en) | 2012-04-02 | 2013-10-10 | modeRNA Therapeutics | Modified polynucleotides for the production of membrane proteins |
| US9410173B2 (en) | 2012-10-24 | 2016-08-09 | Clontech Laboratories, Inc. | Template switch-based methods for producing a product nucleic acid |
| ES2921623T3 (es) | 2012-11-26 | 2022-08-30 | Modernatx Inc | ARN modificado terminalmente |
| JP2016510601A (ja) * | 2013-03-15 | 2016-04-11 | インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド | 修飾ＲＮＡモノマーを用いたＲＮａｓｅＨ系アッセイ |
| US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
| US9255265B2 (en) | 2013-03-15 | 2016-02-09 | Illumina, Inc. | Methods for producing stranded cDNA libraries |
| WO2015048744A2 (en) | 2013-09-30 | 2015-04-02 | Moderna Therapeutics, Inc. | Polynucleotides encoding immune modulating polypeptides |
| KR20160067219A (ko) | 2013-10-03 | 2016-06-13 | 모더나 세라퓨틱스, 인코포레이티드 | 저밀도 지단백질 수용체를 암호화하는 폴리뉴클레오타이드 |
| CN114507715B (zh) | 2014-11-20 | 2025-01-07 | 杭州智灵龙生物科技有限公司 | 用于核酸扩增的组合物和方法 |
| GB2536446B (en) * | 2015-03-17 | 2020-06-03 | Salisbury Nhs Found Trust | PCR method |
| US11066704B2 (en) | 2016-06-10 | 2021-07-20 | Seegene, Inc. | Methods for preparing tagging oligonucleotides |
| CN120796437A (zh) * | 2017-04-17 | 2025-10-17 | 吉复生物科技有限公司 | 用于制备核酸文库的方法、组合物和试剂盒 |
| FR3067355A1 (fr) | 2017-06-08 | 2018-12-14 | Compagnie Generale Des Etablissements Michelin | Composition de caoutchouc |
| FR3067353B1 (fr) | 2017-06-08 | 2020-10-23 | Centre Nat Rech Scient | Copolymere fonctionnel d'un 1,3-diene et d'une olefine |
| MX2021003847A (es) | 2018-10-04 | 2021-05-27 | Bluestar Genomics Inc | Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual. |
| FR3087443B3 (fr) | 2018-10-23 | 2020-10-23 | Michelin & Cie | Composition de caoutchouc |
| US12529047B1 (en) | 2021-12-21 | 2026-01-20 | Modernatx, Inc. | mRNA quantification methods |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
| JP3337748B2 (ja) | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法 |
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| AU3644499A (en) * | 1998-04-16 | 1999-11-01 | Vanderbilt University | Methods and compositions for high yield production of eukaryotic proteins |
| ATE330031T1 (de) * | 1998-07-31 | 2006-07-15 | Gen Probe Inc | Reversibel inhibierende sonden |
| US6132997A (en) * | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
| GB0118758D0 (en) * | 2001-08-01 | 2001-09-26 | Sybesma Wilbert F H | Chimeric primed based real time rt-pcr for qualification of rna or dna templates in a crude sample |
| US20050153333A1 (en) * | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| ATE446384T1 (de) * | 2004-04-01 | 2009-11-15 | Genisphere Inc | Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates |
-
2005
- 2005-11-30 AT AT05814417T patent/ATE551349T1/de active
- 2005-11-30 AU AU2005338632A patent/AU2005338632B2/en not_active Expired
- 2005-11-30 CA CA2639819A patent/CA2639819C/en not_active Expired - Lifetime
- 2005-11-30 EP EP05814417A patent/EP1954706B1/de not_active Expired - Lifetime
- 2005-11-30 US US12/095,409 patent/US8304183B2/en active Active
- 2005-11-30 WO PCT/CA2005/001830 patent/WO2007062495A1/en not_active Ceased
-
2012
- 2012-09-26 US US13/627,616 patent/US20130029881A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8304183B2 (en) | 2012-11-06 |
| CA2639819A1 (en) | 2007-06-07 |
| AU2005338632A1 (en) | 2007-06-07 |
| US20090227009A1 (en) | 2009-09-10 |
| WO2007062495A1 (en) | 2007-06-07 |
| CA2639819C (en) | 2012-10-23 |
| EP1954706A1 (de) | 2008-08-13 |
| US20130029881A1 (en) | 2013-01-31 |
| EP1954706A4 (de) | 2009-11-18 |
| AU2005338632B2 (en) | 2010-05-20 |
| EP1954706B1 (de) | 2012-03-28 |
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