ATE469984T1 - Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon - Google Patents

Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon

Info

Publication number
ATE469984T1
ATE469984T1 AT04812460T AT04812460T ATE469984T1 AT E469984 T1 ATE469984 T1 AT E469984T1 AT 04812460 T AT04812460 T AT 04812460T AT 04812460 T AT04812460 T AT 04812460T AT E469984 T1 ATE469984 T1 AT E469984T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid molecules
molecules containing
recombination site
relates
Prior art date
Application number
AT04812460T
Other languages
English (en)
Inventor
Jon Chesnut
Louis Leong
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE469984T1 publication Critical patent/ATE469984T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT04812460T 2003-12-01 2004-12-01 Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon ATE469984T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52567203P 2003-12-01 2003-12-01
PCT/US2004/039935 WO2005054438A2 (en) 2003-12-01 2004-12-01 Nucleic acid molecules containing recombination sites and methods of using the same

Publications (1)

Publication Number Publication Date
ATE469984T1 true ATE469984T1 (de) 2010-06-15

Family

ID=34652371

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04812460T ATE469984T1 (de) 2003-12-01 2004-12-01 Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon

Country Status (6)

Country Link
US (2) US8304189B2 (de)
EP (2) EP1697534B1 (de)
JP (1) JP2007512838A (de)
AT (1) ATE469984T1 (de)
DE (1) DE602004027538D1 (de)
WO (1) WO2005054438A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
DE69836092T2 (de) * 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
DE60042969D1 (de) 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US7696335B2 (en) * 2005-10-13 2010-04-13 Bc Cancer Agency Kits for multiple non-cross reacting recombination reactions utilizing loxP sequences
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2011105429A1 (ja) * 2010-02-24 2011-09-01 学校法人日本大学 部位特異的組換え方法及びキット
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
EP2625526B1 (de) 2010-10-04 2017-03-15 Genapsys Inc. Systeme und verfahren für automatisierte und wiederverwendbare parallele biologische reaktionen
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
EP2971141B1 (de) 2013-03-15 2018-11-28 Genapsys, Inc. Systeme für biologische analysen
EP3080300B1 (de) 2013-12-11 2020-09-02 Genapsys Inc. Systeme und verfahren für biologische analysen und berechnungen
EP3132060B1 (de) 2014-04-18 2019-03-13 Genapsys Inc. Verfahren und systeme zur nukleinsäureamplifikation
EP3488017A4 (de) 2016-07-20 2020-02-26 Genapsys Inc. Systeme und verfahren zur nukleinsäuresequenzierung
EP3684951A4 (de) 2017-09-21 2021-06-16 Genapsys, Inc. Systeme und verfahren zur nukleinsäuresequenzierung
CN116064626A (zh) * 2022-09-07 2023-05-05 中国农业科学院都市农业研究所 一种可组装超大片段的高效多基因组装载体系统、其构建方法及多基因组装方法

Family Cites Families (516)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US462505A (en) 1891-11-03 cusiiman
US153100A (en) 1874-07-14 Improvement in reversible window-sashes
US4045420A (en) 1973-05-29 1977-08-30 Syva Company Non-oxo-carbonyl containing benzoyl ecgonine and cocaine compounds polypeptide and derivatives thereof
US4331808A (en) 1978-07-24 1982-05-25 Miles Laboratories, Inc. Chemiluminescent naphthalene-1,2-dicarboxylic acid hydrazide-labeled haptens
US4216245A (en) 1978-07-25 1980-08-05 Miles Laboratories, Inc. Method of making printed reagent test devices
US4293652A (en) 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
US5844072A (en) * 1992-05-26 1998-12-01 University Of California Antibiotic cryptdin peptides and methods of their use
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US5097097A (en) * 1980-07-30 1992-03-17 Abbott Laboratories Triazinylaminofluoresceins
US4420568A (en) 1980-07-30 1983-12-13 Abbott Laboratories Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4670572A (en) 1981-07-01 1987-06-02 Eastman Kodak Company Phenolic fluorescent labels
US4668640A (en) 1981-12-11 1987-05-26 Abbott Laboratories Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins
US4585862A (en) 1981-12-11 1986-04-29 Abbott Laboratories Fluorescence polarization immunoassay
JPS58136536A (ja) 1982-02-08 1983-08-13 Ichikoh Ind Ltd 電動式リモ−トコントロ−ルミラ−
US4525048A (en) 1983-03-21 1985-06-25 W. Haking Enterprises, Limited Automatic slot positioning device for camera take-up spool
US4661450A (en) 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
US4745051A (en) 1983-05-27 1988-05-17 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
US4517338A (en) 1983-06-20 1985-05-14 Chiron Corporation Multiple reactor system and method for polynucleotide synthesis
US4879239A (en) 1983-11-03 1989-11-07 American Type Culture Collection Method of culturing freeze-dried microorganisms and resultant preparation
US4626505A (en) 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
US5120653A (en) 1985-04-08 1992-06-09 Microgenics Corporation Vector comprising DNA sequence coding for enzyme-donor polypeptide
EP0178314A1 (de) 1984-04-26 1986-04-23 The Australian National University Klonierungsverfahren für doppelstrang-dns und dessen verwendung bei der herstellung viraler genklone
US4673640A (en) 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
US4859587A (en) 1984-06-04 1989-08-22 Institut Merieux Recombinant herpes simplex viruses, vaccines and methods
US4736866B1 (en) 1984-06-22 1988-04-12 Transgenic non-human mammals
DK365785A (da) 1984-09-17 1986-03-18 Hoffmann La Roche Metalcomplexer
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4743546A (en) 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
US5200333A (en) 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
US4797368A (en) * 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5422096A (en) 1985-05-08 1995-06-06 The General Hospital Corporation Hydroxy-aryl metal chelates for diagnostic NMR imaging
DE3688920D1 (de) * 1985-07-03 1993-09-30 Genencor Int Hybride Polypeptide und Verfahren zu deren Herstellung.
US6121043A (en) 1985-09-06 2000-09-19 Syntro Corporation Recombinant herpesvirus of turkeys comprising a foreign DNA inserted into a non-essential region of the herpesvirus of turkeys genome
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5125748A (en) 1986-03-26 1992-06-30 Beckman Instruments, Inc. Optical detection module for use in an automated laboratory work station
US5104621A (en) 1986-03-26 1992-04-14 Beckman Instruments, Inc. Automated multi-purpose analytical chemistry processing center and laboratory work station
US5206568A (en) 1986-03-26 1993-04-27 Beckman Instruments, Inc. Coordinated control of stepper motors
US5139744A (en) 1986-03-26 1992-08-18 Beckman Instruments, Inc. Automated laboratory work station having module identification means
US4959217A (en) 1986-05-22 1990-09-25 Syntex (U.S.A.) Inc. Delayed/sustained release of macromolecules
US5262176A (en) 1986-07-03 1993-11-16 Advanced Magnetics, Inc. Synthesis of polysaccharide covered superparamagnetic oxide colloids
US5221623A (en) 1986-07-22 1993-06-22 Boyce Thompson Institute For Plant Research, Inc. Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5173411A (en) 1987-01-14 1992-12-22 President And Fellows Of Harvard College Method for determining the nucleotide base sequence of a DNA molecule
US4942130A (en) 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
NZ225470A (en) 1987-07-21 1990-06-26 Du Pont Stable and viable recombinant viral vectors
US4960707A (en) 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
US5672344A (en) 1987-12-30 1997-09-30 The Regents Of The University Of Michigan Viral-mediated gene transfer system
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
AU611263B2 (en) 1988-02-12 1991-06-06 Commonwealth Scientific And Industrial Research Organisation Pox virus vectors
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5378618A (en) * 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
US5147608A (en) 1988-04-29 1992-09-15 Millipore Corporation Apparatus and process for performing repetitive chemical processing
US5139942A (en) 1988-05-19 1992-08-18 New England Biolabs, Inc. Method for producing the nde i restriction endonuclease and methylase
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
DE3841453A1 (de) 1988-12-09 1990-06-13 Degussa Verfahren zum konjugativen transfer von mobilisierbaren vektoren aus e.coli in gram-positive bakterien und dafuer geeignete vektoren
CA2005252C (en) 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
US5334375A (en) 1988-12-29 1994-08-02 Colgate Palmolive Company Antibacterial antiplaque oral composition
DE3901681A1 (de) 1989-01-21 1990-07-26 Behringwerke Ag Signalpeptid fuer die sekretion von peptiden in escherichia coli, verfahren zu seiner gewinnung und seine verwendung
WO1990008839A1 (en) 1989-02-06 1990-08-09 Eastman Kodak Company Thermostable polymerase dna sequencing reaction concentrate
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
CA2012983A1 (en) 1989-03-27 1990-09-27 Sydney Brenner Process for nucleic acid detection by binary amplification
US5244805A (en) 1989-05-17 1993-09-14 University Of Georgia Research Foundation, Inc. Baculovirus expression vectors
US5252466A (en) 1989-05-19 1993-10-12 Biotechnology Research And Development Corporation Fusion proteins having a site for in vivo post-translation modification and methods of making and purifying them
US5102797A (en) 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6346413B1 (en) * 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5656207A (en) 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
US5077214A (en) 1989-07-07 1991-12-31 The Texas A&M University System Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells
US5162222A (en) 1989-07-07 1992-11-10 Guarino Linda A Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells or recombinant baculoviruses
US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US5155037A (en) 1989-08-04 1992-10-13 The Texas A&M University System Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems
US5047342A (en) 1989-08-10 1991-09-10 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase
US5270179A (en) 1989-08-10 1993-12-14 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity
DE68920335T2 (de) 1989-08-22 1995-06-29 E.I. Du Pont De Nemours & Co., Wilmington, Del. In vitro-verpackungsvorrichtung um dna-fragmente von 95-kb zu klonen.
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5436146A (en) 1989-09-07 1995-07-25 The Trustees Of Princeton University Helper-free stocks of recombinant adeno-associated virus vectors
JP2994010B2 (ja) 1989-10-31 1999-12-27 財団法人相模中央化学研究所 クローニングベクタープラスミド、それから誘導されるベクタープライマー及びそれを用いたｃＤＮＡバンクの作製方法
NO904633L (no) 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
CA2071943C (en) 1989-12-22 2007-04-24 Joan Tellefsen Odell Site-specific recombination of dna in plant cells
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
EP0437071A1 (de) 1990-01-08 1991-07-17 Stratagene Klonierungs-Wirtorganismen
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5242681A (en) 1990-03-12 1993-09-07 Research Corporation Technologies, Inc. Lipophilic contrast agents for diagnostic image analysis
US5082784A (en) * 1990-03-20 1992-01-21 Life Technologies, Inc. Cloned kpni restriction-modification system
US5192675A (en) 1990-03-20 1993-03-09 Life Technologies, Inc. Cloned KpnI restriction-modification system
CA2037349C (en) 1990-03-26 2008-06-17 James G. Wetmur Branch migration of nucleotides
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5098839A (en) 1990-05-10 1992-03-24 New England Biolabs, Inc. Type ii restriction endonuclease obtainable from pseudomonas alcaligenes and a process for producing the same
US5658722A (en) 1990-05-15 1997-08-19 New York Blood Center, Inc. Process for the sterilization of biological compositions using UVA1 irradiation
US5147800A (en) 1990-06-08 1992-09-15 Life Technologies, Inc. Host expressing ngoaiii restriction endonuclease and modification methylase from neisseria
ES2139598T3 (es) 1990-07-10 2000-02-16 Medical Res Council Procedimientos para la produccion de miembros de parejas de union especifica.
GB9015559D0 (en) 1990-07-16 1990-09-05 Beckswift Ltd Heat exchange apparatus
US5179015A (en) * 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
US6074818A (en) 1990-08-24 2000-06-13 The University Of Tennessee Research Corporation Fingerprinting of nucleic acids, products and methods
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
US5169784A (en) 1990-09-17 1992-12-08 The Texas A & M University System Baculovirus dual promoter expression vector
EP0738779B1 (de) 1990-09-27 2004-05-19 Invitrogen Corporation Direkte Klonierung von PCR amplifizierten Nukleinsäuren
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
ATE197718T1 (de) 1990-09-28 2000-12-15 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
US5227288A (en) 1990-10-01 1993-07-13 Blattner Frederick R DNA sequencing vector with reversible insert
WO1992006188A2 (en) 1990-10-05 1992-04-16 Barnes Wayne M Thermostable dna polymerase
US5202248A (en) 1990-11-02 1993-04-13 New England Biolabs, Inc. Method for cloning and producing the nco i restriction endonuclease and methylase
DE69132905T2 (de) 1990-12-06 2002-08-01 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Detektion von Nukleinsäuresequenzen
FR2670502B1 (fr) 1990-12-13 1994-09-16 Eurolysine Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.
US5286632A (en) * 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
US5162209A (en) 1991-01-18 1992-11-10 Beth Israel Hospital Association Synthesis of full-length, double-stranded dna from a single-stranded linear dna template
US5348788A (en) * 1991-01-30 1994-09-20 Interpore Orthopaedics, Inc. Mesh sheet with microscopic projections and holes
DE4104186A1 (de) 1991-02-12 1992-08-13 Genentech Inc Neue, ueber endozytose in hoehere eukaryotische zellen aufnehmbare, nukleinsaeure enthaltende komplexe
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5763170A (en) 1991-04-16 1998-06-09 Amersham International Plc Method for forming an array of biological particles
US5871907A (en) * 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5962255A (en) 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
US5858657A (en) * 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
WO1992022650A1 (en) * 1991-06-17 1992-12-23 Life Technologies, Inc. Recombination-facilitated multiplex analysis of dna fragments
GB9113417D0 (en) 1991-06-21 1991-08-07 Glaverbel Fire-resistant panel
CA2114124A1 (en) 1991-07-24 1993-02-04 Keith Gregg Single step amplification and sequencing of nucleic acids
NZ244306A (en) 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation
IL103059A0 (en) 1991-09-30 1993-02-21 Boehringer Ingelheim Int Conjugates for introducing nucleic acid into higher eucaryotic cells
US6013638A (en) 1991-10-02 2000-01-11 The United States Of America As Represented By The Department Of Health And Human Services Adenovirus comprising deletions on the E1A, E1B and E3 regions for transfer of genes to the lung
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
AU681572B2 (en) 1991-10-21 1997-09-04 Med Immune, Inc. Bacterial expression vectors containing DNA encoding secretion signals of lipoproteins
US5252479A (en) 1991-11-08 1993-10-12 Research Corporation Technologies, Inc. Safe vector for gene therapy
GB9123929D0 (en) 1991-11-11 1992-01-02 Wellcome Found Novel expression system
AU2954992A (en) 1991-11-13 1993-06-15 Mta Kozponti Kemiai Kutato Intezete Process for preparing n-(arylsulphonyl)-carbamide acid derivates and intermediates useful for carrying out this process
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US5670113A (en) 1991-12-20 1997-09-23 Sibia Neurosciences, Inc. Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
EP0552108B1 (de) 1992-01-17 1999-11-10 Lakowicz, Joseph R. Phasenmodulationsenergieübertragungsfluoroimmunassay
EP0626999A4 (en) 1992-01-24 1997-04-09 Life Technologies Inc Modulation of enzyme activities in the -i(in vivo) cloning of dna.
US5667988A (en) 1992-01-27 1997-09-16 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
US5635381A (en) 1992-02-26 1997-06-03 Mogen International Nv Agrobacterium bacteria capable of site-specific recombination
JPH05236997A (ja) 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
EP0656941B1 (de) 1992-03-24 2005-06-01 Cambridge Antibody Technology Limited Verfahren zur herstellung von gliedern von spezifischen bindungspaaren
US5352803A (en) 1992-03-30 1994-10-04 Abbott Laboratories 5(6)-methyl substituted fluorescein derivatives
DE4210963A1 (de) 1992-04-02 1993-10-07 Baxter Deutschland Automatische Vorrichtung zur photometrischen Analyse von flüssigen Proben
AU682308C (en) 1992-04-03 2006-08-17 Regents Of The University Of California, The Self-assembling polynucleotide delivery system
US5231021A (en) 1992-04-10 1993-07-27 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from xanthomonas
US5285173A (en) 1992-07-13 1994-02-08 Analog Devices, Inc. Signal-controlled ring oscillator with delay cells having constant gain with change in frequency
US7176029B2 (en) * 1992-07-31 2007-02-13 Universite Libre De Bruxelles Cloning and/or sequencing vector
BE1006085A3 (fr) 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US5733733A (en) * 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) * 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
IL106760A (en) 1992-08-25 1999-12-31 Miles Inc Protein-nucleic acid hybrid construct for translocating an exogenous nucleic acid into a target cell and nucleus
US5348886A (en) 1992-09-04 1994-09-20 Monsanto Company Method of producing recombinant eukaryotic viruses in bacteria
US6225121B1 (en) 1992-09-14 2001-05-01 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
CA2145278C (en) 1992-09-25 2009-03-10 Commonwealth Scientific And Industrial Research Organisation Target binding polypeptide
JP3337748B2 (ja) 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法
US5355215A (en) 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
CZ285533B6 (cs) 1992-10-02 1999-08-11 Lonza Ag DNA-fragmenty, plasmidy a mikroorganismy obsahující tyto DNA-fragmenty a plasmidy a způsob biotechnologické výroby biotinu
KR950703640A (ko) 1992-10-09 1995-09-20 가일 케이,나우톤. 간 보존 세포(liver reserve cells)
WO1994009127A2 (en) 1992-10-16 1994-04-28 The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services Supercoiled minicircle dna as as a unitary promoter vector
US5445935A (en) 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
EP0673431A1 (de) 1992-12-03 1995-09-27 Genzyme Corporation GENTHERAPIE DER MYKOVISCIDOSES (kystic fibroses)
ATE199392T1 (de) * 1992-12-04 2001-03-15 Medical Res Council Multivalente und multispezifische bindungsproteine, deren herstellung und verwendung
US5248605A (en) 1992-12-07 1993-09-28 Life Technologies, Inc. Cloning and expressing restriction endonucleases from haemophilus
GB2273623B (en) 1992-12-15 1996-12-04 Northern Telecom Ltd Optical transmission system
US5334575A (en) 1992-12-17 1994-08-02 Eastman Kodak Company Dye-containing beads for laser-induced thermal dye transfer
US5312746A (en) 1993-01-08 1994-05-17 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from caryophanon
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
AU671820B2 (en) 1993-02-10 1996-09-12 Promega Corporation Non-radioactive DNA sequencing
US5368823A (en) 1993-02-11 1994-11-29 University Of Georgia Research Foundation, Inc. Automated synthesis of oligonucleotides
GB9302798D0 (en) 1993-02-12 1993-03-31 Medical Res Council Improvements in or relating to cloning dna
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
AU6359894A (en) 1993-03-05 1994-09-26 Crop Genetics International Corporation Agricultural-chemical-producing endosymbiotic microorganisms and method of preparing and using same
WO1994023751A1 (de) 1993-04-14 1994-10-27 Boehringer Mannheim Gmbh Nukleinsäure-transferpeptide und deren verwendung zur einschleusung von nukleinsäuren in eukaryontische zellen
WO1994024296A2 (en) 1993-04-19 1994-10-27 University Of Saskatchewan Recombinant bovine herpesvirus type 1 vaccines
US5334526A (en) 1993-05-28 1994-08-02 Life Technologies, Inc. Cloning and expression of AluI restriction endonuclease
FR2705686B1 (fr) * 1993-05-28 1995-08-18 Transgene Sa Nouveaux adénovirus défectifs et lignées de complémentation correspondantes.
US6133028A (en) 1993-05-28 2000-10-17 Transgene S.A. Defective adenoviruses and corresponding complementation lines
US6051427A (en) 1993-06-11 2000-04-18 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US5834256A (en) 1993-06-11 1998-11-10 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
DE4319468C1 (de) 1993-06-11 1994-11-17 Mueller Manfred W Verfahren zum Herstellen eines zu einem Ribonukleinsäure (RNA)-Molekül komplementären Desoxyribonukleinsäure(DNA)-Stranges (cDNA-Stranges), sowie die Verwendung des Verfahrens zur Analyse von RNA-Molekülen
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
WO1994029450A2 (en) 1993-06-15 1994-12-22 E.I. Du Pont De Nemours And Company Novel, recombinantly produced spider silk analogs
US5919676A (en) 1993-06-24 1999-07-06 Advec, Inc. Adenoviral vector system comprising Cre-loxP recombination
US6140087A (en) 1993-06-24 2000-10-31 Advec, Inc. Adenovirus vectors for gene therapy
EP0705344B8 (de) 1993-06-24 2006-05-10 Advec Inc. Adenovirus vektoren für gentherapie
US6120764A (en) 1993-06-24 2000-09-19 Advec, Inc. Adenoviruses for control of gene expression
US5350564A (en) 1993-06-28 1994-09-27 Baxter Diagnostics Inc. Automated chemical analyzer with apparatus and method for conveying and temporary storage of sample tubes
EP0632054A1 (de) 1993-06-28 1995-01-04 European Molecular Biology Laboratory Regulation stellenspezifischer Rekombination mittels stellungsspezifischer Rekombinase/Kernrezeptor-Fusionsproteine
US5843744A (en) 1993-07-08 1998-12-01 Ecogen Inc. Bacillus thuringiensis Tn5401 proteins
US5441884A (en) 1993-07-08 1995-08-15 Ecogen Inc. Bacillus thuringiensis transposon TN5401
CA2163364C (en) 1993-07-14 2009-10-27 Francis C. Szoka, Jr. Self-assembling polynucleotide delivery system comprising dendrimer polycations
EP0917235B1 (de) 1993-08-24 2003-01-22 Matsushita Electric Industrial Co., Ltd. Geschichtete dielektrische Antennenweiche
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5624803A (en) 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
CA2174324A1 (en) 1993-10-21 1995-04-27 Katsuya Mukae Pernasal composition and pernasal preparation containing the same
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5470740A (en) 1993-11-04 1995-11-28 Life Technologies, Inc. Cloned NsiI restriction-modification system
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2177367A1 (en) 1993-12-03 1995-06-08 Andrew David Griffiths Recombinant binding proteins and peptides
DE9318862U1 (de) 1993-12-10 1995-04-06 Danz, Robert, Dipl.-Ing., 71101 Schönaich Halterung für eine punktförmige, biegemomentfreie Lagerung von Mehrfach-Isolierglasscheiben
US5674908A (en) 1993-12-20 1997-10-07 Life Technologies, Inc. Highly packed polycationic ammonium, sulfonium and phosphonium lipids
US5861273A (en) 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US5470727A (en) 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells
FR2715940B1 (fr) 1994-02-10 1996-04-26 Pasteur Merieux Serums Vacc Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5492841A (en) * 1994-02-18 1996-02-20 E. I. Du Pont De Nemours And Company Quaternary ammonium immunogenic conjugates and immunoassay reagents
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995032225A1 (en) 1994-05-23 1995-11-30 The Salk Institute For Biological Studies Method for site-specific integration of nucleic acids and related products
US5714353A (en) 1994-05-24 1998-02-03 Research Corporation Technologies, Inc. Safe vectors for gene therapy
EP0784690B1 (de) 1994-06-10 2006-08-16 Genvec, Inc. Adenoviren-vektor systeme und zelllinien
US5807746A (en) 1994-06-13 1998-09-15 Vanderbilt University Method for importing biologically active molecules into cells
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5622821A (en) 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
ATE226642T1 (de) 1994-07-07 2002-11-15 Tno Modifizierte proenzyme als substrate für proteolische enzyme
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
GB9415369D0 (en) 1994-07-29 1994-09-21 Lynxvale Ltd Mutant virus
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5627024A (en) 1994-08-05 1997-05-06 The Scripps Research Institute Lambdoid bacteriophage vectors for expression and display of foreign proteins
US6800452B1 (en) 1994-08-08 2004-10-05 Science Applications International Corporation Automated methods for simultaneously performing a plurality of signal-based assays
US5589618A (en) 1994-09-01 1996-12-31 University Of Florida Materials and methods for increasing corn seed weight
US5534428A (en) 1994-09-06 1996-07-09 Life Technologies, Inc. Cloned Ssti/SacI restriction-modification system
JP4216350B2 (ja) 1994-09-19 2009-01-28 大日本住友製薬株式会社 動物細胞感染用の組換えｄｎａウイルスベクター
US5601980A (en) * 1994-09-23 1997-02-11 Hewlett-Packard Company Manufacturing method and apparatus for biological probe arrays using vision-assisted micropipetting
JP3031178B2 (ja) 1994-09-28 2000-04-10 株式会社村田製作所 複合高周波部品
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5605802A (en) * 1994-09-30 1997-02-25 The Salk Institute For Biological Studies Method for identifying agents which block infection of cells by HIV
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
DE69400567T2 (de) 1994-10-17 1997-02-06 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit veränderter Nukleotid-Bindungstelle
US5777079A (en) 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
FI972225L (fi) 1994-11-28 1997-07-16 Genetic Therapy Inc Vektorit kudosspesifistä replikaatiota varten
US5998205A (en) 1994-11-28 1999-12-07 Genetic Therapy, Inc. Vectors for tissue-specific replication
FR2727689A1 (fr) 1994-12-01 1996-06-07 Transgene Sa Nouveau procede de preparation d'un vecteur viral
US5589351A (en) 1994-12-06 1996-12-31 Nps Pharmaceuticals, Inc. Fluorescence detection apparatus
US5786139A (en) 1994-12-09 1998-07-28 Panvera Corporation Method and kit for detecting nucleic acid cleavage utilizing a covalently attached fluorescent tag
US5811274A (en) 1994-12-09 1998-09-22 The Regents Of The University Of Michigan Methods, compositions and apparatus for cell transfection
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5736388A (en) 1994-12-30 1998-04-07 Chada; Sunil Bacteriophage-mediated gene transfer systems capable of transfecting eukaryotic cells
IL112263A (en) 1995-01-05 1999-09-22 Eatech Advanced Food Technolog Edible utensils and methods for producing them
IL116816A (en) 1995-01-20 2003-05-29 Rhone Poulenc Rorer Sa Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof
US5981177A (en) 1995-01-25 1999-11-09 Demirjian; David C. Protein fusion method and constructs
JP3256973B2 (ja) 1995-02-03 2002-02-18 株式会社デンソー 燃料供給装置
FR2730504B1 (fr) 1995-02-13 1997-03-28 Rhone Poulenc Rorer Sa Procede de preparation de genomes d'adenovirus recombinants
FR2731014B1 (fr) 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
US5599695A (en) * 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5814300A (en) 1995-03-03 1998-09-29 Cephalon, Inc. Gene-targeted non-human mammals deficient in the SOD-1 gene
US5741657A (en) 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6135061A (en) 1995-04-04 2000-10-24 Srp 687 Pty Ltd. Air inlets for water heaters
US5695971A (en) 1995-04-07 1997-12-09 Amresco Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production
EP0830459B1 (de) 1995-04-11 2007-01-24 The General Hospital Corporation Reverse "two-hybrid"-systeme
FR2733762B1 (fr) 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
US5741715A (en) 1995-05-30 1998-04-21 Roche Diagnostic Systems, Inc. Quinidine immunoassay and reagents
CA2222744C (en) 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6300118B1 (en) 1995-06-07 2001-10-09 American Home Products Corporation Plasmids comprising a genetically altered feline immunodeficiency virus genome
EP0937098B1 (de) 1995-06-07 2002-08-14 Invitrogen Corporation Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5877021A (en) * 1995-07-07 1999-03-02 Ribozyme Pharmaceuticals, Inc. B7-1 targeted ribozymes
US6455254B1 (en) 1995-07-18 2002-09-24 Diversa Corporation Sequence based screening
FR2737222B1 (fr) * 1995-07-24 1997-10-17 Transgene Sa Nouveaux vecteurs viraux et lignee pour la therapie genique
US5851826A (en) 1995-07-26 1998-12-22 Children's Medical Center Corporation Helper virus-free herpesvirus vector packaging system
WO1997006265A2 (en) 1995-08-07 1997-02-20 The Perkin-Elmer Corporation Recombinant clone selection system
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
US6218128B1 (en) 1997-09-12 2001-04-17 Allergan Sales, Inc. Methods of identifying compounds having nuclear receptor negative hormone and/or antagonist activities
AUPN523995A0 (en) 1995-09-05 1995-09-28 Crc For Biopharmaceutical Research Pty Ltd Method for producing phage display vectors
DE69628026T2 (de) * 1995-09-07 2004-04-08 Novozymes A/S Nachweis der aktivität von enzymen für detergenzien durch phagen.
AU7236296A (en) 1995-09-08 1997-03-27 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
US20030027296A1 (en) * 1995-09-08 2003-02-06 Deb K. Chatterjee Cloned dna polymerases from thermotoga maritima and mutants thereof
US6013516A (en) * 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
AU716781B2 (en) 1995-10-13 2000-03-09 Purdue Research Foundation Method for the production of hybrid plants
EP0859835A2 (de) * 1995-10-17 1998-08-26 Genencor International, Inc. Enzym-array und herstellungsverfahren dafür
US5922535A (en) 1995-11-07 1999-07-13 Huo; Li Identifying sequence differences in nucleic acid populations
US5804395A (en) 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6358709B1 (en) * 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6171820B1 (en) * 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6479258B1 (en) 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6361974B1 (en) * 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
WO1997025446A1 (en) 1996-01-05 1997-07-17 Genetic Therapy, Inc. Recombinase-mediated generation of adenoviral vectors
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6228646B1 (en) 1996-03-07 2001-05-08 The Regents Of The University Of California Helper-free, totally defective adenovirus for gene therapy
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
JP3346976B2 (ja) * 1996-03-18 2002-11-18 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
US5721435A (en) * 1996-04-09 1998-02-24 Hewlett Packard Company Methods and apparatus for measuring optical properties of biological and chemical substances
WO1997040722A1 (en) 1996-04-30 1997-11-06 Jan Sehnoutek Assembling shelf system
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
EP0915903A4 (de) 1996-06-17 2002-09-04 Biodynamics Associates Methode und kits zur herstellung von multikomponent-nukleinsäure-konstrukten
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5710248A (en) * 1996-07-29 1998-01-20 University Of Iowa Research Foundation Peptide tag for immunodetection and immunopurification
NO963263D0 (no) 1996-08-05 1996-08-05 Shelter Container System As Anordning for sammenföyning av isocontainere
US5762936A (en) 1996-09-04 1998-06-09 Biotics Research Corporation Antioxidant derived from lentil and its preparation and uses
GB9618477D0 (en) 1996-09-04 1996-10-16 Univ Leeds Gene therapy
WO1998010086A1 (en) 1996-09-06 1998-03-12 Trustees Of The University Of Pennsylvania Methods using cre-lox for production of recombinant adeno-associated viruses
GB9618835D0 (en) 1996-09-10 1996-10-23 Chiroscience Ltd Process
US5846721A (en) 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
US6025192A (en) * 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
GB2322131B (en) * 1996-09-21 2000-12-20 Viromedica Pacific Ltd Improved retroviral vectors for gene therapy
US5735862A (en) 1996-09-23 1998-04-07 Jennings; Erwin Reeves Semi-automatic suturing and sewing device
US5746997A (en) 1996-10-02 1998-05-05 Epoch Pharmaceuticals, Inc. Radiohalogenation of oligonucleotides via trialkylstannylaryl conjugates
AU4984697A (en) 1996-10-11 1998-05-11 The Texas A & M University System Methods for the generation of primordial germ cells and transgenic animal species
US5955604A (en) 1996-10-15 1999-09-21 The Regents Of The University Of California Substrates for β-lactamase and uses thereof
US5962303A (en) 1996-10-15 1999-10-05 Smithkline Beecham Corporation Topoisomerase III
JP3441899B2 (ja) 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
US6066778A (en) 1996-11-06 2000-05-23 The Regents Of The University Of Michigan Transgenic mice expressing APC resistant factor V
US5942422A (en) 1996-11-14 1999-08-24 The Trustees Of Columbia University In The City Of New York Method for generating a directed, recombinant fusion nucleic acid
WO1998020967A1 (en) 1996-11-14 1998-05-22 Affymetrix, Inc. Chemical amplification for the synthesis of patterned arrays
EP0968284B1 (de) * 1996-11-20 2006-12-13 Introgen Therapeutics, Inc. Ein verbessertes verfahren zur produktion und reinigung von adenoviralen vektoren
US6255060B1 (en) 1996-11-21 2001-07-03 Trustees Of The University Of Pennsylvania Method of detecting protein by immuno RNA
US6395524B2 (en) 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
US5800999A (en) 1996-12-16 1998-09-01 Tropix, Inc. Dioxetane-precursor-labeled probes and detection assays employing the same
US6303301B1 (en) 1997-01-13 2001-10-16 Affymetrix, Inc. Expression monitoring for gene function identification
US6576752B1 (en) 1997-02-14 2003-06-10 Isis Pharmaceuticals, Inc. Aminooxy functionalized oligomers
US6410220B1 (en) 1997-02-28 2002-06-25 Nature Technology Corp Self-assembling genes, vectors and uses thereof
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6071742A (en) 1997-03-05 2000-06-06 Board Of Regents Of The University Of Nebraska Coxsackie virus as a vector for delivery of anti-inflammatory cytokines
US5981182A (en) 1997-03-13 1999-11-09 Albert Einstein College Of Medicine Of Yeshiva University Vector constructs for the selection and identification of open reading frames
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US5917012A (en) 1997-04-03 1999-06-29 Wako Pure Chemical Industries, Ltd. Peptide derivatives
US6057098A (en) 1997-04-04 2000-05-02 Biosite Diagnostics, Inc. Polyvalent display libraries
US5981275A (en) 1997-04-14 1999-11-09 Genzyme Corporation Transgene expression system for increased persistence
CA2287542A1 (en) 1997-04-22 1998-10-29 Invitrogen Corporation Methods for the production of aslv reverse transcriptases composed of multiple subunits
US6410311B1 (en) 1997-05-09 2002-06-25 Schering-Plough Veterinary Corporation Recombinant feline herpesvirus comprising a foreign DNA inserted into a region corresponding to a 3.0 kb EcoRI-SalI fragment of a feline herpesvirus genome
DE19720839B4 (de) 1997-05-17 2007-08-16 Dirk Dr. Schindelhauer Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay
JP2002504820A (ja) 1997-06-04 2002-02-12 スミスクライン・ビーチャム・コーポレイション 全長ｃＤＮＡの迅速クローニング法
CA2263716C (en) 1997-06-12 2007-09-25 Sloan-Kettering Institute For Cancer Research Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase
US5856144A (en) 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments
US6066776A (en) 1997-07-16 2000-05-23 Atrium Medical Corporation Self-forming prosthesis for repair of soft tissue defects
US6484183B1 (en) 1997-07-25 2002-11-19 Affymetrix, Inc. Method and system for providing a polymorphism database
US5989872A (en) 1997-08-12 1999-11-23 Clontech Laboratories, Inc. Methods and compositions for transferring DNA sequence information among vectors
US6140086A (en) 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
GB9717953D0 (en) * 1997-08-22 1997-10-29 Smithkline Beecham Biolog Vaccine
US6054295A (en) 1997-08-27 2000-04-25 Merck & Co., Inc. DNA molecules encoding human nuclear receptor proteins
US7135608B1 (en) 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
US5888795A (en) 1997-09-09 1999-03-30 Becton, Dickinson And Company Thermostable uracil DNA glycosylase and methods of use
JP4304348B2 (ja) 1997-09-22 2009-07-29 独立行政法人理化学研究所 Ｄｎａの単離方法
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
JP3189758B2 (ja) 1997-09-30 2001-07-16 日本電気株式会社 符号化装置
CA2305711A1 (en) 1997-10-07 1999-04-15 Merck & Co., Inc. Assays for nuclear receptor ligands using fret
US6054271A (en) 1997-10-21 2000-04-25 The Regents Of The University Of California Methods of using synthetic molecules and target sequences
US5932474A (en) 1997-10-21 1999-08-03 The Regents Of The University Of California Target sequences for synthetic molecules
US6008378A (en) 1997-10-21 1999-12-28 The Regents Of The University Of California Synthetic molecules that specifically react with target sequences
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
DE69836092T2 (de) 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
CN101125873A (zh) 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
EP0966528A1 (de) 1997-11-07 1999-12-29 Iconix Pharmaceuticals, Inc. Surrogat-genetik zur charakterisierung von zielgenen
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
AU745960C (en) 1997-11-18 2003-09-18 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign DNA into eukaryoticgenomes
US5874259A (en) * 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector
US7101681B1 (en) 1997-11-21 2006-09-05 Amgen, Inc. Nuclear hormone receptor drug screens
ES2337027T3 (es) * 1997-12-05 2010-04-20 Europaisches Laboratorium Fur Molekularbiologie (Embl) Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli.
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
ES2290979T3 (es) 1997-12-15 2008-02-16 Csl Behring Gmbh Cebador marcado para uso en la deteccion de acidos nucleicos diana.
DE69822206T2 (de) 1997-12-19 2005-02-17 Affymetrix, Inc., Santa Clara Erkenntnisse der genomforschung für die suche nach neuartigen wirkstoffen
US6265546B1 (en) 1997-12-22 2001-07-24 Genset Prostate cancer gene
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6150147A (en) 1998-02-06 2000-11-21 Affymetrix, Inc. Biological array fabrication methods with reduction of static charge
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
CN101818145A (zh) 1998-03-20 2010-09-01 联邦科学和工业研究组织 控制基因表达
US6436707B1 (en) 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6080576A (en) 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
DE69938128D1 (de) 1998-03-28 2008-03-27 Univ Utah Res Found Zielgerichtete antisense-bibliotheken
US6018877A (en) 1998-04-16 2000-02-01 Greene; Todd M. Versatile finger retained razor
US6024192A (en) * 1998-04-20 2000-02-15 Griffin; Maurice B. Ladder vise
WO1999055886A1 (en) 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery
WO1999055851A2 (en) 1998-04-28 1999-11-04 Novartis Ag Site-directed transformation of plants
US6197584B1 (en) * 1998-05-01 2001-03-06 Isis Pharmaceuticals, Inc. Antisense modulation of CD40 expression
US6088214A (en) 1998-06-01 2000-07-11 Motorola, Inc. Voltage variable capacitor array and method of manufacture thereof
US6413776B1 (en) * 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
US6248520B1 (en) 1998-07-06 2001-06-19 The Rockefeller University Nucleic acid molecules encoding nuclear hormone receptor coactivators and uses thereof
SE9802454D0 (sv) 1998-07-08 1998-07-08 Pharmacia & Upjohn Ab Production of peptides
US6031094A (en) * 1998-07-23 2000-02-29 The Regents Of The University Of California Beta-lactam substrates and uses thereof
GB9816761D0 (en) * 1998-07-31 1998-09-30 Phogen Limited Herpesvirus preparations and their uses
US6190908B1 (en) * 1998-08-12 2001-02-20 The Scripps Research Institute Modulation of polypeptide display on modified filamentous phage
US6072046A (en) 1998-08-26 2000-06-06 Epoch Pharmaceuticals, Inc. Diaziridinyl-aryl and bis-[di(chloroethyl)amino]-aryl oligonucleotide conjugates and reagents for making the same
WO2000012687A1 (en) 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6227620B1 (en) 1998-09-02 2001-05-08 James H. Page Forward mounted asphalt road mill apparatus
WO2000015779A2 (en) 1998-09-15 2000-03-23 Yale University Molecular cloning using rolling circle amplification
AU1721600A (en) 1998-11-13 2000-06-05 Life Technologies, Inc. Compositions and methods for recombinational cloning of nucleic acid molecules
US6258536B1 (en) 1998-12-01 2001-07-10 Jonathan Oliner Expression monitoring of downstream genes in the BRCA1 pathway
US6452612B1 (en) 1998-12-18 2002-09-17 Parkervision, Inc. Real time video production system and method
US6576463B1 (en) 1999-01-15 2003-06-10 The Regents Of The University Of California Hybrid vectors for gene therapy
IL128111A (en) 1999-01-18 2007-03-08 Yissum Res Dev Co A method of silencing expression of a target sequence in a plant genome
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
WO2000049035A1 (en) 1999-02-19 2000-08-24 The General Hospital Corporation Gene silencing
DE60042969D1 (de) 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
WO2000052141A1 (en) 1999-03-02 2000-09-08 Invitrogen Corporation Cells resistant to toxic genes and uses thereof
AU3391900A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Encryption of traits using split gene sequences
EP1165768A1 (de) 1999-03-19 2002-01-02 Invitrogen Corporation Methode zur herstellung nucleinsäuresequenzen voller länge mittels topoisomerase iii aus e. coli und ihren homologen
US6630333B1 (en) 1999-03-23 2003-10-07 Invitrogen Corporation Substantially pure reverse transriptases and methods of production thereof
WO2000056914A1 (en) 1999-03-24 2000-09-28 Gene Therapy Systems, Inc. Method for generating transcriptionally active dna fragments
FI105584B (fi) * 1999-03-30 2000-09-15 Valmet Corp Menetelmä paperi- tai kartonkikoneen telan valmistamiseksi ja paperi- tai kartonkikoneen tela
AU4205500A (en) 1999-04-06 2000-10-23 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
ES2316363T3 (es) 1999-04-20 2009-04-16 Bayer Bioscience N.V. Metodos para administracion de rna inhibidor a las plantas y aplicaciones de los mismos.
JP2002542821A (ja) 1999-04-30 2002-12-17 ハイブリジェニックス・ソシエテ・アノニム ツーハイブリッドシステムのための原核ｄｎａコレクション、ヘリコバクター・ピロリのタンパク質−タンパク質相互作用およびその用途
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
US6410317B1 (en) 1999-07-14 2002-06-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
DE60044142D1 (de) 1999-07-23 2010-05-20 Univ California Dna-rekombination in eukaryotischen zellen mittels des rekombinationszentrums vom bakteriophag phic31
WO2001011058A1 (en) 1999-08-09 2001-02-15 Monsanto Technology Llc Novel cloning methods and vectors
US6090590A (en) 1999-08-10 2000-07-18 The Regents Of The University Of California Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts
US6476209B1 (en) 2000-11-28 2002-11-05 Genesis Research & Development Corporation Ltd. Polynucleotides, materials incorporating them, and methods for using them
JP2001136045A (ja) 1999-08-23 2001-05-18 Murata Mfg Co Ltd 積層型複合電子部品
DE19941186A1 (de) * 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
WO2001020015A1 (en) 1999-09-17 2001-03-22 Whitehead Institute For Biomedical Research Reverse transfection method
GB9923558D0 (en) 1999-10-05 1999-12-08 Oxford Biomedica Ltd Producer cell
US6656082B1 (en) 1999-10-12 2003-12-02 Toyota Jidosha Kabushiki Kaisha Hybrid vehicle and method of controlling the same
JP4487347B2 (ja) 1999-10-21 2010-06-23 株式会社三洋物産 遊技機
AU784522B2 (en) 1999-10-25 2006-04-27 Invitrogen Corporation Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
US6361958B1 (en) 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
US6277710B1 (en) 1999-11-15 2001-08-21 Chartered Semiconductor Manufacturing Ltd. Method of forming shallow trench isolation
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
ATE391789T1 (de) 1999-12-10 2008-04-15 Invitrogen Corp Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
US6831160B1 (en) 2000-01-24 2004-12-14 The Regents Of The University Of California Method of affinity purifying proteins using modified bis-arsenical fluorescein
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
WO2001062892A2 (en) 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
US7078501B2 (en) 2000-02-25 2006-07-18 Invitrogen Corporation Topoisomerase linker-mediated amplification methods
JP2003526367A (ja) 2000-03-16 2003-09-09 ジェネティカ インコーポレイテッド Ｒｎａ干渉の方法とｒｎａ干渉組成物
US6316608B1 (en) 2000-03-20 2001-11-13 Incyte Genomics, Inc. Combined polynucleotide sequence as discrete assay endpoints
US20020025561A1 (en) * 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
AU2001257078A1 (en) 2000-04-18 2001-10-30 Iconix Pharmaceuticals, Inc. Vector and method for targeted replacement and disruption of an integrated dna sequence
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20020068290A1 (en) 2000-05-31 2002-06-06 Timur Yarovinsky Topoisomerase activated oligonucleotide adaptors and uses therefor
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
GB0016747D0 (en) 2000-07-08 2000-08-30 Johnson Matthey Plc Ink
CA2417130A1 (en) 2000-07-25 2002-01-31 National Research Council Of Canada Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases
US20020146741A1 (en) 2000-07-28 2002-10-10 Halbleib Cale M. Processes for receptpor screening
US6916632B2 (en) 2000-08-21 2005-07-12 Invitrogen Corporation Methods and reagents for molecular cloning
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP2004537712A (ja) * 2000-10-18 2004-12-16 バーチャル・アレイズ・インコーポレーテッド 多重細胞分析システム
WO2002042447A2 (en) 2000-11-03 2002-05-30 Regents Of The University Of Michigan Surface transfection and expression procedure
US6902933B2 (en) 2000-11-03 2005-06-07 Regents Of The University Of Michigan Surface transfection and expression procedure
US6544782B1 (en) 2000-11-13 2003-04-08 Synthegen Systems pREM: a positive selection vector system for direct PCR cloning
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
EP2105496B1 (de) 2000-12-08 2013-02-20 Life Technologies Corporation Verfahren und Zusammensetzungen zur Synthese von Nukleinsäuremolekülen unter Verwendung multipler Erkennungsstellen
NZ526194A (en) 2000-12-08 2004-10-29 Invitrogen Corp Methods of covalently linking, in one or both strands, two or more double stranded nucleotide sequences using one or more topoisomerases
US20060008817A1 (en) * 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
US20070196838A1 (en) 2000-12-08 2007-08-23 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002053576A1 (en) 2001-01-05 2002-07-11 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic dna inserts
AU2002311756A1 (en) 2001-01-18 2002-10-28 Clontech Laboratories, Inc. Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same
JP2004530421A (ja) 2001-02-07 2004-10-07 インヴィトロジェン コーポレーション Ｔｅｒ部位およびｔｅｒ結合タンパク質
US6566067B2 (en) 2001-02-14 2003-05-20 Synthegen Systems, Inc. High fidelity PCR cloning
US20030077804A1 (en) 2001-04-19 2003-04-24 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
ES2338767T3 (es) 2001-04-20 2010-05-12 Novozymes A/S Variantes de lipoxigenasa y su uso.
US7774408B2 (en) 2001-04-23 2010-08-10 Foundationip, Llc Methods, systems, and emails to link emails to matters and organizations
AU2002257243A1 (en) 2001-05-04 2002-11-18 Cornell Research Foundation, Inc. Rapidly cleavable sumo fusion protein expression system for difficult to express proteins
JP2002331431A (ja) 2001-05-08 2002-11-19 Pascal Corp ワークパレット
CA2448505A1 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
WO2003000932A1 (en) 2001-06-20 2003-01-03 Dahlia Minc-Golomb Method for identifying genes that are upstream regulators of other genes of interest
AU2002365110A1 (en) * 2001-07-10 2003-07-15 Massachusetts Institute Of Technology Small molecule microarrays
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US10590418B2 (en) 2001-07-23 2020-03-17 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
US6838285B2 (en) * 2001-09-18 2005-01-04 Becton Dickinson Site specific recombinase based method for producing adenoviral vectors
US6670129B2 (en) 2001-09-24 2003-12-30 Corning Incorporated Cell transfection apparatus and methods for making and using the cell transfection apparatus
US20030135888A1 (en) 2001-09-26 2003-07-17 Tong Zhu Genes that are modulated by posttranscriptional gene silencing
US20030219800A1 (en) 2001-10-18 2003-11-27 Beske Oren E. Multiplexed cell transfection using coded carriers
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
AU2002343792A1 (en) * 2001-11-28 2003-06-10 Center For Advanced Science And Technology Incubation, Ltd. siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
US20030102346A1 (en) 2001-12-04 2003-06-05 Mei-Lien Chen Pneumatic tool system operation and carrier belt
SE523240C2 (sv) 2001-12-12 2004-04-06 Akzo Nobel Nv Användning av hydroxyetylsubstituerad amin som korrosionsinhibitor i saltvattenhaltig miljö i oljefältsapplikationer
US7294504B1 (en) 2001-12-27 2007-11-13 Allele Biotechnology & Pharmaceuticals, Inc. Methods and compositions for DNA mediated gene silencing
AU2003209054A1 (en) 2002-02-07 2003-09-02 Discovery Genomics, Inc. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation and methods of use thereof
US6751110B2 (en) 2002-03-08 2004-06-15 Micron Technology, Inc. Static content addressable memory cell
US7175806B2 (en) * 2002-03-15 2007-02-13 Deal Jeffery L C-band disinfector
AU2003273995A1 (en) 2002-06-05 2003-12-22 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20040132133A1 (en) 2002-07-08 2004-07-08 Invitrogen Corporation Methods and compositions for the production, identification and purification of fusion proteins
US20040219516A1 (en) 2002-07-18 2004-11-04 Invitrogen Corporation Viral vectors containing recombination sites
AU2003257109A1 (en) 2002-08-05 2004-02-23 Invitrogen Corporation Compositions and methods for molecular biology
US20040253620A1 (en) 2003-04-30 2004-12-16 Invitrogen Corporation Use of site specific recombination to prepare molecular markers
WO2004108897A2 (en) 2003-06-02 2004-12-16 Cytokinetics, Inc. Sirna libraries
WO2005028615A2 (en) 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
JP2005044844A (ja) 2003-07-23 2005-02-17 Toshiba Corp 不揮発性半導体記憶装置及びその製造方法
US20050054847A1 (en) 2003-08-01 2005-03-10 Invitrogen Corporation Compositions and methods for preparing short RNA molecules and other nucleic acids
US20050069929A1 (en) 2003-08-08 2005-03-31 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US20050104027A1 (en) 2003-10-17 2005-05-19 Lazarev Pavel I. Three-dimensional integrated circuit with integrated heat sinks
WO2005054427A2 (en) 2003-10-22 2005-06-16 Invitrogen Corporation Target sequences for synthetic molecules
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US20060204979A1 (en) 2004-12-01 2006-09-14 Invitrogen Corporation Reverse two-hybrid system for identification of interaction domains
US9516099B1 (en) 2014-09-23 2016-12-06 Amazon Technologies, Inc. Controlling access to digital items based on weather conditions
KR102423818B1 (ko) 2015-12-18 2022-07-21 삼성전자주식회사 정전척 어셈블리 및 그를 포함하는 반도체 제조장치, 그리고 정전척 온도 측정방법

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