ATE469984T1 - Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon - Google Patents

Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon

Info

Publication number
ATE469984T1
ATE469984T1 AT04812460T AT04812460T ATE469984T1 AT E469984 T1 ATE469984 T1 AT E469984T1 AT 04812460 T AT04812460 T AT 04812460T AT 04812460 T AT04812460 T AT 04812460T AT E469984 T1 ATE469984 T1 AT E469984T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid molecules
molecules containing
recombination site
relates
Prior art date
Application number
AT04812460T
Other languages
English (en)
Inventor
Jon Chesnut
Louis Leong
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE469984T1 publication Critical patent/ATE469984T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT04812460T 2003-12-01 2004-12-01 Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon ATE469984T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52567203P 2003-12-01 2003-12-01
PCT/US2004/039935 WO2005054438A2 (en) 2003-12-01 2004-12-01 Nucleic acid molecules containing recombination sites and methods of using the same

Publications (1)

Publication Number Publication Date
ATE469984T1 true ATE469984T1 (de) 2010-06-15

Family

ID=34652371

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04812460T ATE469984T1 (de) 2003-12-01 2004-12-01 Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon

Country Status (6)

Country Link
US (2) US8304189B2 (de)
EP (2) EP1697534B1 (de)
JP (1) JP2007512838A (de)
AT (1) ATE469984T1 (de)
DE (1) DE602004027538D1 (de)
WO (1) WO2005054438A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
JP4303418B2 (ja) 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
EP2119788A1 (de) 1999-03-02 2009-11-18 Life Technologies Corporation Zusammensetzungen und Verfahren zur Verwendung beim Rekombinationsklonen von Nukleinsäuren
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
JP2009524406A (ja) * 2005-10-13 2009-07-02 ビーシー キャンサー エージェンシー 合成生物学および代謝工学のためのモジュラー型ゲノム
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2011105429A1 (ja) * 2010-02-24 2011-09-01 学校法人日本大学 部位特異的組換え方法及びキット
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
JP6193252B2 (ja) 2011-12-01 2017-09-06 ジナプシス インコーポレイテッド 高効率電子配列決定及び検出のためのシステム並びに方法
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
EP3792921A1 (de) 2013-12-11 2021-03-17 Genapsys, Inc. Systeme und verfahren für biologische analysen und berechnungen
WO2015161054A2 (en) 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CN116064626A (zh) * 2022-09-07 2023-05-05 中国农业科学院都市农业研究所 一种可组装超大片段的高效多基因组装载体系统、其构建方法及多基因组装方法

Family Cites Families (516)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US153100A (en) 1874-07-14 Improvement in reversible window-sashes
US462505A (en) 1891-11-03 cusiiman
US4045420A (en) 1973-05-29 1977-08-30 Syva Company Non-oxo-carbonyl containing benzoyl ecgonine and cocaine compounds polypeptide and derivatives thereof
US4331808A (en) 1978-07-24 1982-05-25 Miles Laboratories, Inc. Chemiluminescent naphthalene-1,2-dicarboxylic acid hydrazide-labeled haptens
US4216245A (en) 1978-07-25 1980-08-05 Miles Laboratories, Inc. Method of making printed reagent test devices
US4293652A (en) 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
US5844072A (en) * 1992-05-26 1998-12-01 University Of California Antibiotic cryptdin peptides and methods of their use
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4420568A (en) 1980-07-30 1983-12-13 Abbott Laboratories Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins
US5097097A (en) * 1980-07-30 1992-03-17 Abbott Laboratories Triazinylaminofluoresceins
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4670572A (en) 1981-07-01 1987-06-02 Eastman Kodak Company Phenolic fluorescent labels
US4585862A (en) 1981-12-11 1986-04-29 Abbott Laboratories Fluorescence polarization immunoassay
US4668640A (en) 1981-12-11 1987-05-26 Abbott Laboratories Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins
JPS58136536A (ja) 1982-02-08 1983-08-13 Ichikoh Ind Ltd 電動式リモ−トコントロ−ルミラ−
US4525048A (en) 1983-03-21 1985-06-25 W. Haking Enterprises, Limited Automatic slot positioning device for camera take-up spool
US4661450A (en) 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
US4745051A (en) 1983-05-27 1988-05-17 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
US4517338A (en) 1983-06-20 1985-05-14 Chiron Corporation Multiple reactor system and method for polynucleotide synthesis
US4879239A (en) 1983-11-03 1989-11-07 American Type Culture Collection Method of culturing freeze-dried microorganisms and resultant preparation
US4626505A (en) 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
US5120653A (en) 1985-04-08 1992-06-09 Microgenics Corporation Vector comprising DNA sequence coding for enzyme-donor polypeptide
WO1985004898A1 (en) 1984-04-26 1985-11-07 The Australian National University An improved method of cloning double-stranded rna, and its use in the production of viral gene clones
US4673640A (en) 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
US4859587A (en) 1984-06-04 1989-08-22 Institut Merieux Recombinant herpes simplex viruses, vaccines and methods
US4736866B1 (en) 1984-06-22 1988-04-12 Transgenic non-human mammals
DK365785A (da) 1984-09-17 1986-03-18 Hoffmann La Roche Metalcomplexer
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4743546A (en) 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
US5200333A (en) 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
US4797368A (en) * 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5422096A (en) 1985-05-08 1995-06-06 The General Hospital Corporation Hydroxy-aryl metal chelates for diagnostic NMR imaging
ATE93541T1 (de) * 1985-07-03 1993-09-15 Genencor Int Hybride polypeptide und verfahren zu deren herstellung.
US6121043A (en) 1985-09-06 2000-09-19 Syntro Corporation Recombinant herpesvirus of turkeys comprising a foreign DNA inserted into a non-essential region of the herpesvirus of turkeys genome
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5139744A (en) 1986-03-26 1992-08-18 Beckman Instruments, Inc. Automated laboratory work station having module identification means
US5206568A (en) 1986-03-26 1993-04-27 Beckman Instruments, Inc. Coordinated control of stepper motors
US5104621A (en) 1986-03-26 1992-04-14 Beckman Instruments, Inc. Automated multi-purpose analytical chemistry processing center and laboratory work station
US5125748A (en) 1986-03-26 1992-06-30 Beckman Instruments, Inc. Optical detection module for use in an automated laboratory work station
US4959217A (en) 1986-05-22 1990-09-25 Syntex (U.S.A.) Inc. Delayed/sustained release of macromolecules
US5262176A (en) 1986-07-03 1993-11-16 Advanced Magnetics, Inc. Synthesis of polysaccharide covered superparamagnetic oxide colloids
US5221623A (en) 1986-07-22 1993-06-22 Boyce Thompson Institute For Plant Research, Inc. Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5173411A (en) 1987-01-14 1992-12-22 President And Fellows Of Harvard College Method for determining the nucleotide base sequence of a DNA molecule
US4942130A (en) 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
EP0300422B1 (de) 1987-07-21 1992-12-02 The Du Pont Merck Pharmaceutical Company Verfahren für die Herstellung von in tierischen Zellen stabilen und lebensfähigen rekombinanten viralen Vektoren
US4960707A (en) 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
US5672344A (en) 1987-12-30 1997-09-30 The Regents Of The University Of Michigan Viral-mediated gene transfer system
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
ATE115187T1 (de) 1988-02-12 1994-12-15 Commw Scient Ind Res Org Poxvirus-vektoren.
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5378618A (en) * 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
US5147608A (en) 1988-04-29 1992-09-15 Millipore Corporation Apparatus and process for performing repetitive chemical processing
US5139942A (en) 1988-05-19 1992-08-18 New England Biolabs, Inc. Method for producing the nde i restriction endonuclease and methylase
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
DE3841453A1 (de) 1988-12-09 1990-06-13 Degussa Verfahren zum konjugativen transfer von mobilisierbaren vektoren aus e.coli in gram-positive bakterien und dafuer geeignete vektoren
CA2005252C (en) 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
US5334375A (en) 1988-12-29 1994-08-02 Colgate Palmolive Company Antibacterial antiplaque oral composition
DE3901681A1 (de) 1989-01-21 1990-07-26 Behringwerke Ag Signalpeptid fuer die sekretion von peptiden in escherichia coli, verfahren zu seiner gewinnung und seine verwendung
JPH0630640B2 (ja) 1989-02-06 1994-04-27 イーストマン コダック カンパニー 熱安定性ポリメラーゼｄｎａシーケエンシング反応濃厚物
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
CA2012983A1 (en) 1989-03-27 1990-09-27 Sydney Brenner Process for nucleic acid detection by binary amplification
US5244805A (en) 1989-05-17 1993-09-14 University Of Georgia Research Foundation, Inc. Baculovirus expression vectors
US5252466A (en) 1989-05-19 1993-10-12 Biotechnology Research And Development Corporation Fusion proteins having a site for in vivo post-translation modification and methods of making and purifying them
US5102797A (en) 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
US6346413B1 (en) * 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5656207A (en) 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
US5162222A (en) 1989-07-07 1992-11-10 Guarino Linda A Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells or recombinant baculoviruses
US5077214A (en) 1989-07-07 1991-12-31 The Texas A&M University System Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells
US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US5155037A (en) 1989-08-04 1992-10-13 The Texas A&M University System Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems
US5047342A (en) 1989-08-10 1991-09-10 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase
US5270179A (en) 1989-08-10 1993-12-14 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
JPH05503626A (ja) 1989-08-22 1993-06-17 イー・アイ・デュポン・ドゥ・ヌムール・アンド・カンパニー ９５ｋｂの大きさのｄｎａ断片をクローニングするための生体外ヘッドフルパッケージング系
US5436146A (en) 1989-09-07 1995-07-25 The Trustees Of Princeton University Helper-free stocks of recombinant adeno-associated virus vectors
JP2994010B2 (ja) 1989-10-31 1999-12-27 財団法人相模中央化学研究所 クローニングベクタープラスミド、それから誘導されるベクタープライマー及びそれを用いたｃＤＮＡバンクの作製方法
NO904633L (no) 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
WO1991009957A1 (en) 1989-12-22 1991-07-11 E.I. Du Pont De Nemours And Company Site-specific recombination of dna in plant cells
ATE201047T1 (de) 1990-01-08 2001-05-15 Stratagene Inc Neue wirtsorganismen zur klonierung
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5242681A (en) 1990-03-12 1993-09-07 Research Corporation Technologies, Inc. Lipophilic contrast agents for diagnostic image analysis
US5192675A (en) 1990-03-20 1993-03-09 Life Technologies, Inc. Cloned KpnI restriction-modification system
US5082784A (en) 1990-03-20 1992-01-21 Life Technologies, Inc. Cloned kpni restriction-modification system
CA2037349C (en) 1990-03-26 2008-06-17 James G. Wetmur Branch migration of nucleotides
AU7791991A (en) 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5098839A (en) * 1990-05-10 1992-03-24 New England Biolabs, Inc. Type ii restriction endonuclease obtainable from pseudomonas alcaligenes and a process for producing the same
US5658722A (en) 1990-05-15 1997-08-19 New York Blood Center, Inc. Process for the sterilization of biological compositions using UVA1 irradiation
US5147800A (en) 1990-06-08 1992-09-15 Life Technologies, Inc. Host expressing ngoaiii restriction endonuclease and modification methylase from neisseria
CA2109602C (en) 1990-07-10 2002-10-01 Gregory P. Winter Methods for producing members of specific binding pairs
GB9015559D0 (en) 1990-07-16 1990-09-05 Beckswift Ltd Heat exchange apparatus
US5179015A (en) * 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
US6074818A (en) 1990-08-24 2000-06-13 The University Of Tennessee Research Corporation Fingerprinting of nucleic acids, products and methods
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
US5169784A (en) 1990-09-17 1992-12-08 The Texas A & M University System Baculovirus dual promoter expression vector
DE69133559T2 (de) 1990-09-27 2007-11-22 Invitrogen Corp., Carlsbad Direkte Klonierung von PCR amplifizierten Nucleinsäuren
EP0894860B1 (de) 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostabile DNA Polymerasen und deren Deletionsmutanten
AU658378B2 (en) 1990-09-28 1995-04-13 F. Hoffmann-La Roche Ag Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus
US5227288A (en) 1990-10-01 1993-07-13 Blattner Frederick R DNA sequencing vector with reversible insert
AU8906091A (en) 1990-10-05 1992-04-28 Wayne M. Barnes Thermostable dna polymerase
US5202248A (en) 1990-11-02 1993-04-13 New England Biolabs, Inc. Method for cloning and producing the nco i restriction endonuclease and methylase
WO1992010588A1 (en) 1990-12-06 1992-06-25 Affymax Technologies N.V. Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
FR2670502B1 (fr) 1990-12-13 1994-09-16 Eurolysine Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.
US5286632A (en) * 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
US5162209A (en) 1991-01-18 1992-11-10 Beth Israel Hospital Association Synthesis of full-length, double-stranded dna from a single-stranded linear dna template
US5348788A (en) * 1991-01-30 1994-09-20 Interpore Orthopaedics, Inc. Mesh sheet with microscopic projections and holes
DE4104186A1 (de) 1991-02-12 1992-08-13 Genentech Inc Neue, ueber endozytose in hoehere eukaryotische zellen aufnehmbare, nukleinsaeure enthaltende komplexe
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5763170A (en) 1991-04-16 1998-06-09 Amersham International Plc Method for forming an array of biological particles
US5962255A (en) 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
US5871907A (en) * 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5858657A (en) * 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
WO1992022650A1 (en) * 1991-06-17 1992-12-23 Life Technologies, Inc. Recombination-facilitated multiplex analysis of dna fragments
GB9113417D0 (en) 1991-06-21 1991-08-07 Glaverbel Fire-resistant panel
CA2114124A1 (en) 1991-07-24 1993-02-04 Keith Gregg Single step amplification and sequencing of nucleic acids
IL103059A0 (en) 1991-09-30 1993-02-21 Boehringer Ingelheim Int Conjugates for introducing nucleic acid into higher eucaryotic cells
NZ244306A (en) 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation
US6013638A (en) 1991-10-02 2000-01-11 The United States Of America As Represented By The Department Of Health And Human Services Adenovirus comprising deletions on the E1A, E1B and E3 regions for transfer of genes to the lung
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
WO1993007897A1 (en) 1991-10-21 1993-04-29 Medimmune, Inc. Bacterial expression vectors containing dna encoding secretion signals of lipoproteins
US5252479A (en) 1991-11-08 1993-10-12 Research Corporation Technologies, Inc. Safe vector for gene therapy
GB9123929D0 (en) 1991-11-11 1992-01-02 Wellcome Found Novel expression system
AU2954992A (en) 1991-11-13 1993-06-15 Mta Kozponti Kemiai Kutato Intezete Process for preparing n-(arylsulphonyl)-carbamide acid derivates and intermediates useful for carrying out this process
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US5670113A (en) 1991-12-20 1997-09-23 Sibia Neurosciences, Inc. Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
CA2087413A1 (en) 1992-01-17 1993-07-18 Joseph R. Lakowicz Fluorescent energy transfer immunoassay
EP0626999A4 (en) 1992-01-24 1997-04-09 Life Technologies Inc Modulation of enzyme activities in the -i(in vivo) cloning of dna.
US5667988A (en) 1992-01-27 1997-09-16 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
ATE201236T1 (de) 1992-02-26 2001-06-15 Zeneca Mogen B V Zur ortsspezifischen rekomination fähige agrobakterium stämme
JPH05236997A (ja) 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
JP3507073B2 (ja) 1992-03-24 2004-03-15 ケンブリッジ アンティボディー テクノロジー リミティド 特異的結合対の成員の製造方法
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5352803A (en) 1992-03-30 1994-10-04 Abbott Laboratories 5(6)-methyl substituted fluorescein derivatives
DE4210963A1 (de) 1992-04-02 1993-10-07 Baxter Deutschland Automatische Vorrichtung zur photometrischen Analyse von flüssigen Proben
DE69333434T2 (de) 1992-04-03 2005-03-03 The Regents Of The University Of California, Oakland Selbstorganisierendes system zur verabreichung von polynukleotiden enthaltend ein amphiphatisches peptid
US5231021A (en) 1992-04-10 1993-07-27 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from xanthomonas
US5285173A (en) 1992-07-13 1994-02-08 Analog Devices, Inc. Signal-controlled ring oscillator with delay cells having constant gain with change in frequency
US7176029B2 (en) * 1992-07-31 2007-02-13 Universite Libre De Bruxelles Cloning and/or sequencing vector
BE1006085A3 (fr) 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US5614389A (en) * 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) * 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
IL106760A (en) 1992-08-25 1999-12-31 Miles Inc Protein-nucleic acid hybrid construct for translocating an exogenous nucleic acid into a target cell and nucleus
US5348886A (en) 1992-09-04 1994-09-20 Monsanto Company Method of producing recombinant eukaryotic viruses in bacteria
US6225121B1 (en) 1992-09-14 2001-05-01 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
EP0672068A4 (de) 1992-09-25 1997-02-26 Commw Scient Ind Res Org Zielmoleküle-bindende polypeptide.
JP3337748B2 (ja) 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法
US5355215A (en) 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
EP0798384A1 (de) 1992-10-02 1997-10-01 Lonza A.G. Biotechnologisches Verfahren zur Herstellung von Biotin
PT671923E (pt) 1992-10-09 2001-10-30 Advanced Tissue Sciences Inc Celulas de reserva do figado
AU5405994A (en) 1992-10-16 1994-05-09 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Supercoiled minicircle dna as as a unitary promoter vector
US5445935A (en) 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
JPH08503855A (ja) 1992-12-03 1996-04-30 ジェンザイム・コーポレイション 嚢胞性線維症に対する遺伝子治療
JP3720353B2 (ja) * 1992-12-04 2005-11-24 メディカル リサーチ カウンシル 多価および多重特異性の結合タンパク質、それらの製造および使用
US5248605A (en) 1992-12-07 1993-09-28 Life Technologies, Inc. Cloning and expressing restriction endonucleases from haemophilus
GB2273623B (en) 1992-12-15 1996-12-04 Northern Telecom Ltd Optical transmission system
US5334575A (en) 1992-12-17 1994-08-02 Eastman Kodak Company Dye-containing beads for laser-induced thermal dye transfer
US5312746A (en) 1993-01-08 1994-05-17 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from caryophanon
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
AU671820B2 (en) 1993-02-10 1996-09-12 Promega Corporation Non-radioactive DNA sequencing
US5368823A (en) 1993-02-11 1994-11-29 University Of Georgia Research Foundation, Inc. Automated synthesis of oligonucleotides
GB9302798D0 (en) 1993-02-12 1993-03-31 Medical Res Council Improvements in or relating to cloning dna
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
AU6359894A (en) 1993-03-05 1994-09-26 Crop Genetics International Corporation Agricultural-chemical-producing endosymbiotic microorganisms and method of preparing and using same
WO1994023751A1 (de) 1993-04-14 1994-10-27 Boehringer Mannheim Gmbh Nukleinsäure-transferpeptide und deren verwendung zur einschleusung von nukleinsäuren in eukaryontische zellen
WO1994024296A2 (en) 1993-04-19 1994-10-27 University Of Saskatchewan Recombinant bovine herpesvirus type 1 vaccines
FR2705686B1 (fr) * 1993-05-28 1995-08-18 Transgene Sa Nouveaux adénovirus défectifs et lignées de complémentation correspondantes.
US6133028A (en) 1993-05-28 2000-10-17 Transgene S.A. Defective adenoviruses and corresponding complementation lines
US5334526A (en) 1993-05-28 1994-08-02 Life Technologies, Inc. Cloning and expression of AluI restriction endonuclease
US6051427A (en) 1993-06-11 2000-04-18 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US5834256A (en) 1993-06-11 1998-11-10 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
DE4319468C1 (de) 1993-06-11 1994-11-17 Mueller Manfred W Verfahren zum Herstellen eines zu einem Ribonukleinsäure (RNA)-Molekül komplementären Desoxyribonukleinsäure(DNA)-Stranges (cDNA-Stranges), sowie die Verwendung des Verfahrens zur Analyse von RNA-Molekülen
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
ATE253635T1 (de) 1993-06-15 2003-11-15 Du Pont Rekombinante spinnerseide analoge
US6140087A (en) 1993-06-24 2000-10-31 Advec, Inc. Adenovirus vectors for gene therapy
US6120764A (en) 1993-06-24 2000-09-19 Advec, Inc. Adenoviruses for control of gene expression
US5919676A (en) 1993-06-24 1999-07-06 Advec, Inc. Adenoviral vector system comprising Cre-loxP recombination
ATE304604T1 (de) 1993-06-24 2005-09-15 Frank L Graham Adenovirus vektoren für gentherapie
US5350564A (en) 1993-06-28 1994-09-27 Baxter Diagnostics Inc. Automated chemical analyzer with apparatus and method for conveying and temporary storage of sample tubes
EP0632054A1 (de) * 1993-06-28 1995-01-04 European Molecular Biology Laboratory Regulation stellenspezifischer Rekombination mittels stellungsspezifischer Rekombinase/Kernrezeptor-Fusionsproteine
US5843744A (en) 1993-07-08 1998-12-01 Ecogen Inc. Bacillus thuringiensis Tn5401 proteins
US5441884A (en) 1993-07-08 1995-08-15 Ecogen Inc. Bacillus thuringiensis transposon TN5401
AU681735C (en) 1993-07-14 2002-02-21 Regents Of The University Of California, The Self-assembling polynucleotide delivery system comprising dendrimer polycations
DE69432058T2 (de) 1993-08-24 2004-01-22 Matsushita Electric Industrial Co., Ltd., Kadoma Geschichtetes dielektrisches Filter
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5624803A (en) 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
DK0724885T3 (da) 1993-10-21 2002-05-21 Hisamitsu Pharmaceutical Co Pernasal sammensætning og pernasalt præparat indeholdende dette
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5470740A (en) 1993-11-04 1995-11-28 Life Technologies, Inc. Cloned NsiI restriction-modification system
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
WO1995015388A1 (en) 1993-12-03 1995-06-08 Medical Research Council Recombinant binding proteins and peptides
DE4342097A1 (de) 1993-12-10 1995-06-14 Danz Robert Halterung für die biegemomentfreie Lagerung von Glasplatten
US5674908A (en) 1993-12-20 1997-10-07 Life Technologies, Inc. Highly packed polycationic ammonium, sulfonium and phosphonium lipids
US5861273A (en) 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US5470727A (en) 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells
FR2715940B1 (fr) 1994-02-10 1996-04-26 Pasteur Merieux Serums Vacc Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5492841A (en) * 1994-02-18 1996-02-20 E. I. Du Pont De Nemours And Company Quaternary ammonium immunogenic conjugates and immunoassay reagents
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995032225A1 (en) 1994-05-23 1995-11-30 The Salk Institute For Biological Studies Method for site-specific integration of nucleic acids and related products
US5714353A (en) 1994-05-24 1998-02-03 Research Corporation Technologies, Inc. Safe vectors for gene therapy
CA2192442C (en) 1994-06-10 2007-09-25 Imre Kovesdi Complementary adenoviral vector systems and cell lines
US5807746A (en) 1994-06-13 1998-09-15 Vanderbilt University Method for importing biologically active molecules into cells
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5622821A (en) 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
ATE226642T1 (de) 1994-07-07 2002-11-15 Tno Modifizierte proenzyme als substrate für proteolische enzyme
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
GB9415369D0 (en) 1994-07-29 1994-09-21 Lynxvale Ltd Mutant virus
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5627024A (en) 1994-08-05 1997-05-06 The Scripps Research Institute Lambdoid bacteriophage vectors for expression and display of foreign proteins
US6800452B1 (en) 1994-08-08 2004-10-05 Science Applications International Corporation Automated methods for simultaneously performing a plurality of signal-based assays
US5589618A (en) 1994-09-01 1996-12-31 University Of Florida Materials and methods for increasing corn seed weight
US5534428A (en) 1994-09-06 1996-07-09 Life Technologies, Inc. Cloned Ssti/SacI restriction-modification system
JP4216350B2 (ja) 1994-09-19 2009-01-28 大日本住友製薬株式会社 動物細胞感染用の組換えｄｎａウイルスベクター
US5601980A (en) * 1994-09-23 1997-02-11 Hewlett-Packard Company Manufacturing method and apparatus for biological probe arrays using vision-assisted micropipetting
JP3031178B2 (ja) 1994-09-28 2000-04-10 株式会社村田製作所 複合高周波部品
US5605802A (en) * 1994-09-30 1997-02-25 The Salk Institute For Biological Studies Method for identifying agents which block infection of cells by HIV
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
EP0655506B1 (de) 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5777079A (en) 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
EP0791050B1 (de) 1994-11-28 2006-02-15 Cell Genesys Inc. Vektoren zur gewebsspezifischen replikation
US5998205A (en) 1994-11-28 1999-12-07 Genetic Therapy, Inc. Vectors for tissue-specific replication
FR2727689A1 (fr) 1994-12-01 1996-06-07 Transgene Sa Nouveau procede de preparation d'un vecteur viral
US5589351A (en) 1994-12-06 1996-12-31 Nps Pharmaceuticals, Inc. Fluorescence detection apparatus
US5786139A (en) 1994-12-09 1998-07-28 Panvera Corporation Method and kit for detecting nucleic acid cleavage utilizing a covalently attached fluorescent tag
US5811274A (en) 1994-12-09 1998-09-22 The Regents Of The University Of Michigan Methods, compositions and apparatus for cell transfection
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5736388A (en) 1994-12-30 1998-04-07 Chada; Sunil Bacteriophage-mediated gene transfer systems capable of transfecting eukaryotic cells
IL112263A (en) 1995-01-05 1999-09-22 Eatech Advanced Food Technolog Edible utensils and methods for producing them
IL116816A (en) 1995-01-20 2003-05-29 Rhone Poulenc Rorer Sa Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof
US5981177A (en) 1995-01-25 1999-11-09 Demirjian; David C. Protein fusion method and constructs
JP3256972B2 (ja) 1995-02-03 2002-02-18 株式会社デンソー 燃料供給装置
FR2730504B1 (fr) 1995-02-13 1997-03-28 Rhone Poulenc Rorer Sa Procede de preparation de genomes d'adenovirus recombinants
FR2731014B1 (fr) 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
US5599695A (en) * 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5814300A (en) 1995-03-03 1998-09-29 Cephalon, Inc. Gene-targeted non-human mammals deficient in the SOD-1 gene
US5741657A (en) 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6135061A (en) 1995-04-04 2000-10-24 Srp 687 Pty Ltd. Air inlets for water heaters
US5695971A (en) 1995-04-07 1997-12-09 Amresco Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production
NZ502795A (en) 1995-04-11 2001-09-28 Gen Hospital Corp Method of identifying molecular interactions employing counterselection and at least two hybrid molecules or two hybrid systems
FR2733762B1 (fr) 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
US5741715A (en) 1995-05-30 1998-04-21 Roche Diagnostic Systems, Inc. Quinidine immunoassay and reagents
CA2222744C (en) 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
NZ500843A (en) 1995-06-07 2002-03-28 Invitrogen Corp A method of DNA recombination which is selectable to select for cells containing a product and against cells only harbouring the insert donor
US6300118B1 (en) 1995-06-07 2001-10-09 American Home Products Corporation Plasmids comprising a genetically altered feline immunodeficiency virus genome
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5877021A (en) * 1995-07-07 1999-03-02 Ribozyme Pharmaceuticals, Inc. B7-1 targeted ribozymes
US6455254B1 (en) 1995-07-18 2002-09-24 Diversa Corporation Sequence based screening
FR2737222B1 (fr) * 1995-07-24 1997-10-17 Transgene Sa Nouveaux vecteurs viraux et lignee pour la therapie genique
US5851826A (en) 1995-07-26 1998-12-22 Children's Medical Center Corporation Helper virus-free herpesvirus vector packaging system
US5750346A (en) 1995-08-07 1998-05-12 The Perkin-Elmer Corporation Host organism capture
US6218128B1 (en) 1997-09-12 2001-04-17 Allergan Sales, Inc. Methods of identifying compounds having nuclear receptor negative hormone and/or antagonist activities
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
AUPN523995A0 (en) 1995-09-05 1995-09-28 Crc For Biopharmaceutical Research Pty Ltd Method for producing phage display vectors
EP0854933B1 (de) * 1995-09-07 2003-05-07 Novozymes A/S Nachweis der aktivität von enzymen für detergenzien durch phagen.
US5948614A (en) 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US20030027296A1 (en) * 1995-09-08 2003-02-06 Deb K. Chatterjee Cloned dna polymerases from thermotoga maritima and mutants thereof
US6013516A (en) * 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
NZ322673A (en) 1995-10-13 1999-09-29 Purdue Research Foundation Method for the production of hybrid plants
AU726108B2 (en) * 1995-10-17 2000-11-02 Genencor International, Inc. Enzymatic array and process of making same
US5922535A (en) 1995-11-07 1999-07-13 Huo; Li Identifying sequence differences in nucleic acid populations
US5804395A (en) 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6171820B1 (en) * 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6361974B1 (en) * 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6479258B1 (en) 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
AU1695197A (en) 1996-01-05 1997-08-01 Genetic Therapy, Inc. Recombinase-mediated generation of adenoviral vectors
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6228646B1 (en) 1996-03-07 2001-05-08 The Regents Of The University Of California Helper-free, totally defective adenovirus for gene therapy
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
JP3346976B2 (ja) * 1996-03-18 2002-11-18 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
US5721435A (en) * 1996-04-09 1998-02-24 Hewlett Packard Company Methods and apparatus for measuring optical properties of biological and chemical substances
DE19781748T1 (de) 1996-04-30 1999-04-08 Jan Sehnoutek Zusammensetzbares Regalsystem
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
IL127624A0 (en) 1996-06-17 1999-10-28 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5710248A (en) * 1996-07-29 1998-01-20 University Of Iowa Research Foundation Peptide tag for immunodetection and immunopurification
NO963263D0 (no) 1996-08-05 1996-08-05 Shelter Container System As Anordning for sammenföyning av isocontainere
GB9618477D0 (en) 1996-09-04 1996-10-16 Univ Leeds Gene therapy
US5762936A (en) 1996-09-04 1998-06-09 Biotics Research Corporation Antioxidant derived from lentil and its preparation and uses
AU722375B2 (en) 1996-09-06 2000-08-03 Trustees Of The University Of Pennsylvania, The Methods using cre-lox for production of recombinant adeno-associated viruses
GB9618835D0 (en) 1996-09-10 1996-10-23 Chiroscience Ltd Process
US5846721A (en) 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
US6025192A (en) * 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
KR100246096B1 (ko) * 1996-09-21 2000-03-15 이선경 유전자 요법을 위한 개선된 레트로바이러스 벡터
US5735862A (en) 1996-09-23 1998-04-07 Jennings; Erwin Reeves Semi-automatic suturing and sewing device
US5746997A (en) 1996-10-02 1998-05-05 Epoch Pharmaceuticals, Inc. Radiohalogenation of oligonucleotides via trialkylstannylaryl conjugates
WO1998016630A1 (en) 1996-10-11 1998-04-23 The Texas A & M University System Methods for the generation of primordial germ cells and transgenic animal species
US5962303A (en) 1996-10-15 1999-10-05 Smithkline Beecham Corporation Topoisomerase III
US5955604A (en) 1996-10-15 1999-09-21 The Regents Of The University Of California Substrates for β-lactamase and uses thereof
JP3441899B2 (ja) 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
US6066778A (en) 1996-11-06 2000-05-23 The Regents Of The University Of Michigan Transgenic mice expressing APC resistant factor V
US5942422A (en) 1996-11-14 1999-08-24 The Trustees Of Columbia University In The City Of New York Method for generating a directed, recombinant fusion nucleic acid
WO1998020967A1 (en) 1996-11-14 1998-05-22 Affymetrix, Inc. Chemical amplification for the synthesis of patterned arrays
WO1998022588A2 (en) * 1996-11-20 1998-05-28 Introgen Therapeutics, Inc. An improved method for the production and purification of adenoviral vectors
US6255060B1 (en) 1996-11-21 2001-07-03 Trustees Of The University Of Pennsylvania Method of detecting protein by immuno RNA
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US5800999A (en) 1996-12-16 1998-09-01 Tropix, Inc. Dioxetane-precursor-labeled probes and detection assays employing the same
US6303301B1 (en) 1997-01-13 2001-10-16 Affymetrix, Inc. Expression monitoring for gene function identification
US6576752B1 (en) 1997-02-14 2003-06-10 Isis Pharmaceuticals, Inc. Aminooxy functionalized oligomers
WO1998038326A1 (en) 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6071742A (en) 1997-03-05 2000-06-06 Board Of Regents Of The University Of Nebraska Coxsackie virus as a vector for delivery of anti-inflammatory cytokines
US5981182A (en) 1997-03-13 1999-11-09 Albert Einstein College Of Medicine Of Yeshiva University Vector constructs for the selection and identification of open reading frames
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US5917012A (en) 1997-04-03 1999-06-29 Wako Pure Chemical Industries, Ltd. Peptide derivatives
US6057098A (en) 1997-04-04 2000-05-02 Biosite Diagnostics, Inc. Polyvalent display libraries
US5981275A (en) 1997-04-14 1999-11-09 Genzyme Corporation Transgene expression system for increased persistence
EP1005481B1 (de) 1997-04-22 2009-10-14 Life Technologies Corporation Verfahren zur herstellung von aslv reverser transkriptase zusammengestellt aus mehreren untereinheiten
US6410311B1 (en) 1997-05-09 2002-06-25 Schering-Plough Veterinary Corporation Recombinant feline herpesvirus comprising a foreign DNA inserted into a region corresponding to a 3.0 kb EcoRI-SalI fragment of a feline herpesvirus genome
DE19720839B4 (de) 1997-05-17 2007-08-16 Dirk Dr. Schindelhauer Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay
CA2288988A1 (en) 1997-06-04 1998-12-10 Smithkline Beecham Corporation Methods for rapid cloning of full length cdnas
US6653106B1 (en) 1997-06-12 2003-11-25 Sloan-Kettering Institute For Cancer Research Topoisomerase-based ligation and cloning methods
US5856144A (en) 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments
US6066776A (en) 1997-07-16 2000-05-23 Atrium Medical Corporation Self-forming prosthesis for repair of soft tissue defects
WO1999005574A1 (en) 1997-07-25 1999-02-04 Affymetrix, Inc. Method and system for providing a probe array chip design database
US5989872A (en) 1997-08-12 1999-11-23 Clontech Laboratories, Inc. Methods and compositions for transferring DNA sequence information among vectors
US6140086A (en) 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
GB9717953D0 (en) * 1997-08-22 1997-10-29 Smithkline Beecham Biolog Vaccine
US6054295A (en) 1997-08-27 2000-04-25 Merck & Co., Inc. DNA molecules encoding human nuclear receptor proteins
US7135608B1 (en) 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
US5888795A (en) * 1997-09-09 1999-03-30 Becton, Dickinson And Company Thermostable uracil DNA glycosylase and methods of use
JP4304348B2 (ja) 1997-09-22 2009-07-29 独立行政法人理化学研究所 Ｄｎａの単離方法
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
JP3189758B2 (ja) 1997-09-30 2001-07-16 日本電気株式会社 符号化装置
WO1999018124A1 (en) 1997-10-07 1999-04-15 Merck & Co., Inc. Assays for nuclear receptor ligands using fret
US6054271A (en) 1997-10-21 2000-04-25 The Regents Of The University Of California Methods of using synthetic molecules and target sequences
US6008378A (en) 1997-10-21 1999-12-28 The Regents Of The University Of California Synthetic molecules that specifically react with target sequences
US5932474A (en) 1997-10-21 1999-08-03 The Regents Of The University Of California Target sequences for synthetic molecules
JP4303418B2 (ja) 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
CN101125873A (zh) 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
JP2001507581A (ja) 1997-11-07 2001-06-12 アイコニックス・ファーマシューティカルズ・インコーポレーテッド 代用遺伝学的標的解析法
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
WO1999025840A1 (en) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign dna into eukaryoticgenomes
US7101681B1 (en) 1997-11-21 2006-09-05 Amgen, Inc. Nuclear hormone receptor drug screens
US5874259A (en) * 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector
DE69841340D1 (de) * 1997-12-05 2010-01-07 Europ Lab Molekularbiolog Neue Methode zur Klonierung DNS unter Anwendung des E. coli RecE/RecT Rekombinationssystems
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
DE69838210T2 (de) 1997-12-15 2008-05-15 Csl Behring Gmbh Markierter Primer, geeignet für die Detektion von Nukleinsäuren
ATE260989T1 (de) 1997-12-19 2004-03-15 Affymetrix Inc Erkenntnisse der genomforschung für die suche nach neuartigen wirkstoffen
US6265546B1 (en) 1997-12-22 2001-07-24 Genset Prostate cancer gene
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6150147A (en) 1998-02-06 2000-11-21 Affymetrix, Inc. Biological array fabrication methods with reduction of static charge
BRPI9908967B1 (pt) 1998-03-20 2017-05-30 Benitec Australia Ltd processos para reprimir, retardar ou de outro modo reduzir a expressão de um gene alvo em uma célula de planta
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
US6436707B1 (en) 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6080576A (en) 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6586180B1 (en) 1998-03-28 2003-07-01 University Of Utah Directed antisense libraries
US6018877A (en) 1998-04-16 2000-02-01 Greene; Todd M. Versatile finger retained razor
US6024192A (en) * 1998-04-20 2000-02-15 Griffin; Maurice B. Ladder vise
WO1999055886A1 (en) 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery
AU3928899A (en) 1998-04-28 1999-11-16 Novartis Ag Site-directed transformation of plants
US6197584B1 (en) * 1998-05-01 2001-03-06 Isis Pharmaceuticals, Inc. Antisense modulation of CD40 expression
US6088214A (en) 1998-06-01 2000-07-11 Motorola, Inc. Voltage variable capacitor array and method of manufacture thereof
US6413776B1 (en) * 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
US6248520B1 (en) 1998-07-06 2001-06-19 The Rockefeller University Nucleic acid molecules encoding nuclear hormone receptor coactivators and uses thereof
SE9802454D0 (sv) 1998-07-08 1998-07-08 Pharmacia & Upjohn Ab Production of peptides
US6031094A (en) * 1998-07-23 2000-02-29 The Regents Of The University Of California Beta-lactam substrates and uses thereof
GB9816761D0 (en) * 1998-07-31 1998-09-30 Phogen Limited Herpesvirus preparations and their uses
US6190908B1 (en) * 1998-08-12 2001-02-20 The Scripps Research Institute Modulation of polypeptide display on modified filamentous phage
US6072046A (en) 1998-08-26 2000-06-06 Epoch Pharmaceuticals, Inc. Diaziridinyl-aryl and bis-[di(chloroethyl)amino]-aryl oligonucleotide conjugates and reagents for making the same
EP1114148A4 (de) 1998-08-28 2004-12-22 Invitrogen Corp System zur schnellen manipulation von nukleinsäuresequenzen.
US6227620B1 (en) 1998-09-02 2001-05-08 James H. Page Forward mounted asphalt road mill apparatus
CA2342838A1 (en) 1998-09-15 2000-03-23 Yale University Molecular cloning using rolling circle amplification
WO2000029000A1 (en) 1998-11-13 2000-05-25 Life Technologies, Inc. Compositions and methods for recombinational cloning of nucleic acid molecules
US6258536B1 (en) 1998-12-01 2001-07-10 Jonathan Oliner Expression monitoring of downstream genes in the BRCA1 pathway
US6452612B1 (en) 1998-12-18 2002-09-17 Parkervision, Inc. Real time video production system and method
US6576463B1 (en) 1999-01-15 2003-06-10 The Regents Of The University Of California Hybrid vectors for gene therapy
IL128111A (en) 1999-01-18 2007-03-08 Yissum Res Dev Co A method of silencing expression of a target sequence in a plant genome
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
WO2000049035A1 (en) 1999-02-19 2000-08-24 The General Hospital Corporation Gene silencing
EP2119788A1 (de) 1999-03-02 2009-11-18 Life Technologies Corporation Zusammensetzungen und Verfahren zur Verwendung beim Rekombinationsklonen von Nukleinsäuren
EP1159402A4 (de) 1999-03-02 2003-01-29 Invitrogen Corp ZELLEN, WELCHE RESISTENT GEGENüBER TOXISCHEN GENEN SIND UND DEREN ANWENDUNGEN
CA2362737A1 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
EP1165768A1 (de) 1999-03-19 2002-01-02 Invitrogen Corporation Methode zur herstellung nucleinsäuresequenzen voller länge mittels topoisomerase iii aus e. coli und ihren homologen
US6630333B1 (en) 1999-03-23 2003-10-07 Invitrogen Corporation Substantially pure reverse transriptases and methods of production thereof
US6280977B1 (en) 1999-03-24 2001-08-28 Gene Therapy Systems Method for generating transcriptionally active DNA fragments
FI105584B (fi) * 1999-03-30 2000-09-15 Valmet Corp Menetelmä paperi- tai kartonkikoneen telan valmistamiseksi ja paperi- tai kartonkikoneen tela
CA2367396A1 (en) 1999-04-06 2000-10-12 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
AU775188B2 (en) 1999-04-20 2004-07-22 Bayer Cropscience Nv Methods and means for delivering inhibitory RNA to plants and applications thereof
EP1173560A1 (de) 1999-04-30 2002-01-23 Hybrigenics S.A. Ansammlung von prokaryotischen dna für two-hybrid systeme helicobacter pylori protein-protein-wechselwirkungen und verwendung davon
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
CA2373690A1 (en) * 1999-07-14 2001-01-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
AU6364800A (en) 1999-07-23 2001-02-13 Regents Of The University Of California, The Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system
AU6529900A (en) 1999-08-09 2001-03-05 Monsanto Technology Llc Novel cloning methods and vectors
US6090590A (en) 1999-08-10 2000-07-18 The Regents Of The University Of California Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts
US6476209B1 (en) 2000-11-28 2002-11-05 Genesis Research & Development Corporation Ltd. Polynucleotides, materials incorporating them, and methods for using them
JP2001136045A (ja) 1999-08-23 2001-05-18 Murata Mfg Co Ltd 積層型複合電子部品
DE19941186A1 (de) * 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
US20020006664A1 (en) 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
DE60031997D1 (de) 1999-09-17 2007-01-04 Whitehead Biomedical Inst Umkehrtransfektionsverfahren
GB9923558D0 (en) 1999-10-05 1999-12-08 Oxford Biomedica Ltd Producer cell
US6656082B1 (en) 1999-10-12 2003-12-02 Toyota Jidosha Kabushiki Kaisha Hybrid vehicle and method of controlling the same
JP4487347B2 (ja) 1999-10-21 2010-06-23 株式会社三洋物産 遊技機
NZ529476A (en) 1999-10-25 2005-03-24 Invitrogen Corp Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
US6361958B1 (en) 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
US6277710B1 (en) 1999-11-15 2001-08-21 Chartered Semiconductor Manufacturing Ltd. Method of forming shallow trench isolation
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
CN1757724B (zh) 1999-12-10 2014-06-11 茵维特罗根公司 具有独特特异性的多个重组位点在重组克隆中的用途
US6831160B1 (en) 2000-01-24 2004-12-14 The Regents Of The University Of California Method of affinity purifying proteins using modified bis-arsenical fluorescein
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
US7078501B2 (en) 2000-02-25 2006-07-18 Invitrogen Corporation Topoisomerase linker-mediated amplification methods
CA2401632A1 (en) 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
WO2001068836A2 (en) 2000-03-16 2001-09-20 Genetica, Inc. Methods and compositions for rna interference
US6316608B1 (en) 2000-03-20 2001-11-13 Incyte Genomics, Inc. Combined polynucleotide sequence as discrete assay endpoints
US20020025561A1 (en) * 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
WO2001079512A2 (en) 2000-04-18 2001-10-25 Iconix Pharmaceuticals, Inc. Vector and method for targeted replacement and disruption of an integrated dna sequence
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20020068290A1 (en) 2000-05-31 2002-06-06 Timur Yarovinsky Topoisomerase activated oligonucleotide adaptors and uses therefor
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
GB0016747D0 (en) 2000-07-08 2000-08-30 Johnson Matthey Plc Ink
US20040043449A1 (en) 2000-07-25 2004-03-04 Jitao Zou Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases
US20020146741A1 (en) 2000-07-28 2002-10-10 Halbleib Cale M. Processes for receptpor screening
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
ATE315086T1 (de) 2000-08-21 2006-02-15 Invitrogen Corp Methoden und reagenzien für molekulares klonieren
WO2003103600A2 (en) 2002-06-05 2003-12-18 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP2004537712A (ja) * 2000-10-18 2004-12-16 バーチャル・アレイズ・インコーポレーテッド 多重細胞分析システム
EP1330516B1 (de) 2000-11-03 2007-01-17 The Regents Of The University Of Michigan Oberflächentransfektion und expressionsverfahren
US6902933B2 (en) 2000-11-03 2005-06-07 Regents Of The University Of Michigan Surface transfection and expression procedure
US6544782B1 (en) 2000-11-13 2003-04-08 Synthegen Systems pREM: a positive selection vector system for direct PCR cloning
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
US20060008817A1 (en) * 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
JP4394878B2 (ja) 2000-12-08 2010-01-06 ライフ テクノロジーズ コーポレーション 複数の認識部位を用いた核酸分子合成のための方法および組成物
US20070196838A1 (en) 2000-12-08 2007-08-23 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP2004520047A (ja) 2000-12-08 2004-07-08 インヴィトロジェン コーポレーション 組換え型核酸分子を迅速に作製するための組成物と方法
US7214515B2 (en) 2001-01-05 2007-05-08 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic DNA inserts
AU2002311756A1 (en) 2001-01-18 2002-10-28 Clontech Laboratories, Inc. Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same
EP1363935B1 (de) 2001-02-07 2012-11-14 Life Technologies Corporation Ter-stellen und ter-bindende proteine
US6566067B2 (en) 2001-02-14 2003-05-20 Synthegen Systems, Inc. High fidelity PCR cloning
CA2444195A1 (en) 2001-04-19 2002-10-31 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
CA2444735C (en) 2001-04-20 2012-10-23 Novozymes A/S Lipoxygenase
US7774408B2 (en) 2001-04-23 2010-08-10 Foundationip, Llc Methods, systems, and emails to link emails to matters and organizations
DE60238532D1 (de) 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
JP2002331431A (ja) 2001-05-08 2002-11-19 Pascal Corp ワークパレット
JP2004532636A (ja) 2001-05-21 2004-10-28 インヴィトロジェン コーポレーション 核酸分子の単離に用いるための組成物および方法
US7125664B2 (en) 2001-06-20 2006-10-24 Dahlia Minc-Golomb Method for identifying genes that are upstream regulators of other genes of interest
US20030032203A1 (en) * 2001-07-10 2003-02-13 Sabatini David M. Small molecule microarrays
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US10590418B2 (en) 2001-07-23 2020-03-17 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
EP1427816A4 (de) * 2001-09-18 2004-12-01 Clontech Lab Inc Auf stellenspezifischer rekombinase beruhendes verfahren zur herstellung von adenovirusvektoren
US6670129B2 (en) 2001-09-24 2003-12-30 Corning Incorporated Cell transfection apparatus and methods for making and using the cell transfection apparatus
US20030135888A1 (en) 2001-09-26 2003-07-17 Tong Zhu Genes that are modulated by posttranscriptional gene silencing
US20030219800A1 (en) 2001-10-18 2003-11-27 Beske Oren E. Multiplexed cell transfection using coded carriers
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
WO2003046173A1 (en) * 2001-11-28 2003-06-05 Center For Advanced Science And Technology Incubation, Ltd. siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
US20030102346A1 (en) 2001-12-04 2003-06-05 Mei-Lien Chen Pneumatic tool system operation and carrier belt
SE523240C2 (sv) 2001-12-12 2004-04-06 Akzo Nobel Nv Användning av hydroxyetylsubstituerad amin som korrosionsinhibitor i saltvattenhaltig miljö i oljefältsapplikationer
US7294504B1 (en) 2001-12-27 2007-11-13 Allele Biotechnology & Pharmaceuticals, Inc. Methods and compositions for DNA mediated gene silencing
US20030220249A1 (en) 2002-02-07 2003-11-27 Hackett Perry B. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation, and methods of use thereof
US6751110B2 (en) 2002-03-08 2004-06-15 Micron Technology, Inc. Static content addressable memory cell
US7175806B2 (en) * 2002-03-15 2007-02-13 Deal Jeffery L C-band disinfector
US20040132133A1 (en) 2002-07-08 2004-07-08 Invitrogen Corporation Methods and compositions for the production, identification and purification of fusion proteins
US20040219516A1 (en) 2002-07-18 2004-11-04 Invitrogen Corporation Viral vectors containing recombination sites
AU2003257109A1 (en) 2002-08-05 2004-02-23 Invitrogen Corporation Compositions and methods for molecular biology
US20040253620A1 (en) 2003-04-30 2004-12-16 Invitrogen Corporation Use of site specific recombination to prepare molecular markers
WO2004108897A2 (en) 2003-06-02 2004-12-16 Cytokinetics, Inc. Sirna libraries
US20050095615A1 (en) 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
JP2005044844A (ja) 2003-07-23 2005-02-17 Toshiba Corp 不揮発性半導体記憶装置及びその製造方法
WO2005012487A2 (en) 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
US20050069929A1 (en) 2003-08-08 2005-03-31 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US20050104027A1 (en) 2003-10-17 2005-05-19 Lazarev Pavel I. Three-dimensional integrated circuit with integrated heat sinks
US20050176065A1 (en) 2003-10-22 2005-08-11 Invitrogen Corporation Target sequences for synthetic molecules
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2006060595A2 (en) 2004-12-01 2006-06-08 Invitrogen Corporation Reverse two hybrid system for identification of interaction domains
US9516099B1 (en) 2014-09-23 2016-12-06 Amazon Technologies, Inc. Controlling access to digital items based on weather conditions
KR102423818B1 (ko) 2015-12-18 2022-07-21 삼성전자주식회사 정전척 어셈블리 및 그를 포함하는 반도체 제조장치, 그리고 정전척 온도 측정방법

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