ATE66959T1 - Expressionsvektoren zur erhoehten produktion von polypeptiden, diese vektoren enthaltende plasmide, diese plasmide enthaltenden wirte, durch die vektoren hergestellte produkte und entsprechende verfahren. - Google Patents

Expressionsvektoren zur erhoehten produktion von polypeptiden, diese vektoren enthaltende plasmide, diese plasmide enthaltenden wirte, durch die vektoren hergestellte produkte und entsprechende verfahren.

Info

Publication number
ATE66959T1
ATE66959T1 AT84107717T AT84107717T ATE66959T1 AT E66959 T1 ATE66959 T1 AT E66959T1 AT 84107717 T AT84107717 T AT 84107717T AT 84107717 T AT84107717 T AT 84107717T AT E66959 T1 ATE66959 T1 AT E66959T1
Authority
AT
Austria
Prior art keywords
vectors
plasmids
polypeptides
products made
enhanced production
Prior art date
Application number
AT84107717T
Other languages
English (en)
Inventor
Haim Aviv
Marian Gorecki
Avigdor Levanon
Amos Oppenheim
Tikva Vogel
Elisha Zeelon
Menachem Zeevi
Original Assignee
Bio Technology General Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Technology General Corp filed Critical Bio Technology General Corp
Application granted granted Critical
Publication of ATE66959T1 publication Critical patent/ATE66959T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/12—Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Medicines Containing Plant Substances (AREA)
AT84107717T 1983-07-15 1984-07-03 Expressionsvektoren zur erhoehten produktion von polypeptiden, diese vektoren enthaltende plasmide, diese plasmide enthaltenden wirte, durch die vektoren hergestellte produkte und entsprechende verfahren. ATE66959T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51418883A 1983-07-15 1983-07-15
EP84107717A EP0131843B1 (de) 1983-07-15 1984-07-03 Expressionsvektoren zur erhöhten Produktion von Polypeptiden, diese Vektoren enthaltende Plasmide, diese Plasmide enthaltenden Wirte, durch die Vektoren hergestellte Produkte und entsprechende Verfahren

Publications (1)

Publication Number Publication Date
ATE66959T1 true ATE66959T1 (de) 1991-09-15

Family

ID=24046152

Family Applications (2)

Application Number Title Priority Date Filing Date
AT84107717T ATE66959T1 (de) 1983-07-15 1984-07-03 Expressionsvektoren zur erhoehten produktion von polypeptiden, diese vektoren enthaltende plasmide, diese plasmide enthaltenden wirte, durch die vektoren hergestellte produkte und entsprechende verfahren.
AT88121246T ATE136934T1 (de) 1983-07-15 1984-07-03 Wachstumshormon-analoga, pharmazeutische und veterinäre zusammensetzungen, die diese enthalten, sowie methoden für ihre herstellung

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT88121246T ATE136934T1 (de) 1983-07-15 1984-07-03 Wachstumshormon-analoga, pharmazeutische und veterinäre zusammensetzungen, die diese enthalten, sowie methoden für ihre herstellung

Country Status (22)

Country Link
US (3) US4871835A (de)
EP (3) EP0319049B1 (de)
JP (3) JPH0687780B2 (de)
AT (2) ATE66959T1 (de)
AU (2) AU577298B2 (de)
BG (2) BG49718A3 (de)
BR (1) BR8403523A (de)
CA (1) CA1254527A (de)
CZ (1) CZ282867B6 (de)
DD (2) DD229427A5 (de)
DE (2) DE3486425T2 (de)
DK (1) DK347184A (de)
ES (2) ES534321A0 (de)
FI (1) FI90564C (de)
HK (1) HK128095A (de)
HU (1) HU209878B (de)
IE (2) IE64174B1 (de)
IL (8) IL72396A (de)
NZ (2) NZ227962A (de)
RO (1) RO91332B (de)
SK (1) SK278576B6 (de)
ZA (1) ZA845345B (de)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
US5236828A (en) * 1983-07-06 1993-08-17 Papas Takis S Plasmid pJL6
US5670371A (en) * 1983-07-15 1997-09-23 Bio-Technology General Corp. Bacterial expression of superoxide dismutase
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US5332805A (en) * 1984-02-03 1994-07-26 Celltech Limited Process for the recovery of recombinantly produced chymosin from insoluble aggregate
DE3585170D1 (de) * 1984-03-06 1992-02-27 Lilly Co Eli Expressionsvektoren fuer rinderwachstumshormonderivate.
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods
US5162217A (en) * 1984-08-27 1992-11-10 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US5112744A (en) * 1984-08-27 1992-05-12 Bio-Technology General Corp. Plasmids for the production of human Cu-Zn superoxide dismutase, bacterial hosts containing the plasmids and methods for production and recovery of such superoxide dismutase
US5143836A (en) * 1984-08-27 1992-09-01 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
US5759816A (en) * 1984-08-27 1998-06-02 Bio-Technology General Corp. Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
US6030611A (en) * 1984-08-27 2000-02-29 Bio-Technology General Corp. Therapeutic SOD compositions and uses thereof
US4743546A (en) * 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
US4861868A (en) * 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
FR2579223B1 (fr) * 1985-03-25 1988-12-09 Genetica Procede de preparation microbiologique de la serum-albumine humaine
ATE76435T1 (de) 1985-06-26 1992-06-15 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US4788144A (en) * 1985-06-28 1988-11-29 International Minerals & Chemical Corp. Fermentation process for the high level production of swine growth
US4762784A (en) * 1985-07-15 1988-08-09 International Minerals & Chemical Corp. Fermemtation process for the high level production of bovine growth hormone
US4874703A (en) * 1985-08-26 1989-10-17 Eli Lilly And Company Expression vectors for use in E. coli
US5631227A (en) * 1985-09-18 1997-05-20 The Upjohn Company Somatotropin analogs
DE3675382D1 (de) * 1985-09-18 1990-12-06 Upjohn Co Erhoehte biowirkung von saeugetier-somatotropin durch selektive deamidierung.
JPH07110232B2 (ja) * 1985-10-14 1995-11-29 株式会社日立製作所 遺伝子組換え大腸菌の遺伝子生産物を採取する方法および装置
IE59498B1 (en) * 1985-11-22 1994-03-09 Bio Technology General Corp Human manganese superoxide dismutase analog, plasmid for its expression and method of recovering it in enzymatically active form
US6610520B1 (en) 1985-11-22 2003-08-26 Bio-Technology General Corp. Gene encoding human manganese superoxide dismutase and recombinant polypeptide encoded thereby
ES2019874B3 (es) * 1986-10-08 1991-07-16 Upjohn Co Bioactividad mejorada de somatotropina de mamiferos mediante desamidacion selectiva.
US5089406A (en) * 1987-01-07 1992-02-18 Allied-Signal Inc. Method of producing a gene cassette coding for polypeptides with repeating amino acid sequences
GB8701848D0 (en) * 1987-01-28 1987-03-04 Celltech Ltd Polypeptides
US5240837A (en) * 1987-02-19 1993-08-31 The Upjohn Company CDNAS encoding somatotropin, expression vectors and hosts
WO1988006186A2 (en) * 1987-02-19 1988-08-25 The Upjohn Company cDNAs ENCODING SOMATOTROPIN, EXPRESSION VECTORS AND HOSTS
US5104796A (en) * 1987-04-06 1992-04-14 International Minerals & Chemical Corp. High titer production of human somatomedin c
US5047504A (en) * 1987-04-28 1991-09-10 Amgen, Inc. Method for purifying granulocyte-macrophage colony stimulating factor
US4966841A (en) * 1987-05-22 1990-10-30 The Board Of Regents Of The University Of Washington Enhanced vector production and expression of recombinant DNA products
US5030563A (en) * 1987-07-07 1991-07-09 Genetics Institute, Inc. Bacterial hypersecretion using mutant repressor sequence
US5079345A (en) * 1988-08-19 1992-01-07 Eli Lilly And Company Proteins having growth hormone anabolic properties with reduced effect on carbohydrate metabolism
ES2064412T3 (es) * 1988-10-26 1995-02-01 American Cyanamid Co Procedimiento para reducir el contenido de aglomerado de materiales tipo hormona del crecimiento.
EP0397834B1 (de) 1988-10-28 2000-02-02 Genentech, Inc. Verfahren zum nachweis von aktiven domänen und aminosäureresten in polypeptiden und hormonvarianten
US6780613B1 (en) * 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
WO1990005185A1 (fr) * 1988-11-07 1990-05-17 L'universite De L'etat A Liege Hormone de croissance humaine modifiee
US4975529A (en) * 1989-08-18 1990-12-04 Monsanto Company Method of folding somatotropins
US5958879A (en) * 1989-10-12 1999-09-28 Ohio University/Edison Biotechnology Institute Growth hormone receptor antagonists and methods of reducing growth hormone activity in a mammal
US6583115B1 (en) 1989-10-12 2003-06-24 Ohio University/Edison Biotechnology Institute Methods for treating acromegaly and giantism with growth hormone antagonists
IE912345A1 (en) * 1990-08-03 1992-02-12 Pharmacia Ab Treatment of human lactation failure
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5844097A (en) * 1990-11-30 1998-12-01 Monoclonetics International, Inc. Methods for the diagnosis of peripheral nerve damage
EP0559795B1 (de) * 1990-11-30 1995-12-27 Monoclonetics International, Inc. Verfahren zur diagnose chronischer niedriger rückenschmerzen und nackenschmerzen
US5231178A (en) * 1991-01-16 1993-07-27 The Salk Institute Biotechnology/Industrial Associates, Inc. Method for the purification of intact, correctly-folded insulin-like growth factor-1
DE69231467T2 (de) * 1991-05-10 2001-01-25 Genentech, Inc. Auswählen von agonisten und antagonisten von liganden
US5395761A (en) * 1991-11-27 1995-03-07 Eli Lilly And Company Plasmid pHKY334, an expression vector for EK-BGH and host cells transformed therewith
ZA94169B (en) * 1993-01-26 1994-09-07 Lilly Co Eli Non-denaturing potency assay for bovine somatotropin
AU693478B2 (en) * 1994-11-10 1998-07-02 Metabolic Pharmaceuticals Limited Treatment of obesity
US20020142965A1 (en) * 1994-11-15 2002-10-03 Metabolic Pharmaceuticals, Ltd. Treatment of obesity
US6335319B1 (en) 1994-11-15 2002-01-01 Metabolic Pharmaceuticals, Inc. Treatment of obesity
WO1996040203A1 (en) * 1995-06-07 1996-12-19 Ohio University/Edison Biotechnology Institute Methods of treatment using growth hormone antagonists
KR970006498A (ko) * 1995-07-31 1997-02-21 유충식 활성형 인성장호르몬의 정제 방법
AU718439B2 (en) * 1995-09-21 2000-04-13 Genentech Inc. Human growth hormone variants
US5849293A (en) * 1996-01-11 1998-12-15 Cornell Research Foundation, Inc. Use of human transferrin in controlling insulin levels
US6238915B1 (en) 1996-02-13 2001-05-29 Jcr Pharmaceuticals Co., Ltd. Mutant human growth hormones and their uses
NZ502993A (en) 1997-09-08 2002-12-20 Metabolic Pharm Ltd Treatment of obesity using hGH 177-191analogues
DE19951765A1 (de) * 1999-10-27 2001-05-03 Thomas Schweder Wirts-Vektor-Systeme zur Überproduktion von thermolabilen Enzymen psychrophiler Organismen
KR100400638B1 (ko) * 2000-12-06 2003-10-08 주식회사 엘지생명과학 재조합 인간 성장호르몬 유사체의 분리방법
WO2002072847A2 (en) 2001-03-09 2002-09-19 Genentech, Inc. Process for production of polypeptides
CA2498319A1 (en) * 2002-09-09 2004-03-18 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
EP1871795A4 (de) 2005-04-08 2010-03-31 Biogenerix Ag Zusammensetzungen und verfahren zur herstellung von glycosylierungsmutanten eines proteaseresistenten menschlichen wachstumshormons
US20080260820A1 (en) * 2007-04-19 2008-10-23 Gilles Borrelly Oral dosage formulations of protease-resistant polypeptides
EP2446898A1 (de) 2010-09-30 2012-05-02 Laboratorios Del. Dr. Esteve, S.A. Verwendung von Wachstumshormonen zur Verbesserung der Immunantwort bei immunsupprimierten Patienten

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4426323A (en) * 1980-01-10 1984-01-17 Ionics, Incorporated Selected recovery of proteins from fermentation broths
US4443539A (en) * 1980-02-05 1984-04-17 The Upjohn Company Process for preparing bovine growth hormone
IL59690A (en) * 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
FI811009L (fi) * 1980-06-06 1981-12-07 Biogen Nv Foerbaettrade vektorer foerfaranden foer framstaellning av dessa vektorer och foer expression av klonade gener
US4352443A (en) * 1980-06-24 1982-10-05 U.S. Cap & Closure, Inc. Dispenser having a trigger-bulb pump
JPS6045848B2 (ja) * 1980-07-31 1985-10-12 株式会社林原生物化学研究所 ヒト成長ホルモンの製造方法
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
DE3231658A1 (de) 1981-01-21 1983-11-17
EP0061250A3 (de) * 1981-03-23 1983-09-28 Biogen N.V. Verfahren zur Rückgewinnung von Produkten erzeugt durch Wirtorganismen
NZ201918A (en) * 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
US4436815A (en) * 1981-11-27 1984-03-13 Eli Lilly And Company Method for stabilizing and selecting recombinant DNA containing host cells
US4665160A (en) * 1982-03-22 1987-05-12 Genentech, Inc. Novel human growth hormone like protein HGH-V encoded in the human genome
WO1983004418A1 (en) * 1982-06-07 1983-12-22 Celltech Limited A process for the preparation of chymosin
EP0103395A3 (de) * 1982-08-17 1985-05-22 Biogen N.V. DNS-Sequenzen, rekombinante DNS-Moleküle und Verfahren zur Herstellung von rinderwachstumshormonähnlichen Polypeptiden, mit grosser Ausbeute
EP0111814A3 (de) * 1982-12-16 1985-08-14 Mgi Pharma, Inc. Klonierung und Expression von nucleotiden Sequenzen die für Rinderwachstumshormon kodieren
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
JPS60500358A (ja) * 1982-12-23 1985-03-22 アメリカ合衆国 マウスの細胞内で組み換えｄｎａによって生産されたヒト成長ホルモン
US4728609A (en) * 1983-01-10 1988-03-01 Hoffmann-La-Roche Inc. Recombinant growth hormone releasing factor
JPS59145876A (ja) * 1983-02-09 1984-08-21 株式会社リコー 開閉体用のヒンジ
DK162130C (da) * 1983-03-07 1992-03-02 Hoffmann La Roche Grf-analoge peptider, fremgangsmaade til fremstilling deraf, grf-analoge peptider som terapeutiske midler, anvendelse af grf-analoge peptider, fremgangsmaade til fremstilling af farmaceutiske praeparater indeholdende grf-analoge peptider samt parenterale farmaceutiske praeparater indeholdende grf-analoge peptider
ATE107356T1 (de) * 1983-03-25 1994-07-15 Celltech Ltd Verfahren zur herstellung eines proteins.
US4755465A (en) * 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
AU587176B2 (en) * 1984-02-14 1989-08-10 Mycogen Plant Science, Inc. Transfer vector
US4767709A (en) * 1984-06-28 1988-08-30 The Johns Hopkins University Growth-related hormones
DE3426532A1 (de) * 1984-07-18 1986-01-30 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verwendung einer dna-sequenz zur expression sowie dna-struktur und expressionsvektor mit der dna-sequenz
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods
US4612367A (en) * 1985-04-08 1986-09-16 Eli Lilly And Company Process for purifying growth hormone-like materials
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery

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JP2568376B2 (ja) 1997-01-08
IL112178A0 (en) 1995-03-15
EP0319049B1 (de) 1996-04-17
IE64174B1 (en) 1995-07-12
HU209878B (en) 1994-11-28
SK524684A3 (en) 1997-10-08
IE841818L (en) 1985-01-15
EP0691406A1 (de) 1996-01-10
IL108108A0 (en) 1994-04-12
JP3146161B2 (ja) 2001-03-12
IL101629A0 (en) 1992-12-30
EP0319049A3 (en) 1990-01-03
FI90564C (fi) 1994-02-25
ATE136934T1 (de) 1996-05-15
CZ282867B6 (cs) 1997-11-12
US4831120A (en) 1989-05-16
DK347184A (da) 1985-01-16
CZ524684A3 (en) 1997-07-16
DD229427A5 (de) 1985-11-06
EP0131843A1 (de) 1985-01-23
FI842842A7 (fi) 1985-01-16
FI842842A0 (fi) 1984-07-13
BG49721A3 (bg) 1992-01-15
BR8403523A (pt) 1985-06-25
EP0319049A2 (de) 1989-06-07
IL91826A0 (en) 1990-06-10
DE3486425D1 (de) 1996-05-23
ES544976A0 (es) 1986-01-16
RO91332B (ro) 1987-05-01
HUT34543A (en) 1985-03-28
AU3034584A (en) 1985-01-17
CA1254527A (en) 1989-05-23
AU1977788A (en) 1988-12-22
RO91332A (ro) 1987-04-30
US4871835A (en) 1989-10-03
JPH0687780B2 (ja) 1994-11-09
FI90564B (fi) 1993-11-15
ZA845345B (en) 1985-03-27
ES8604252A1 (es) 1986-01-16
NZ208897A (en) 1990-06-26
DE3485003D1 (de) 1991-10-10
ES8602947A1 (es) 1985-12-01
IL91828A (en) 1992-05-25
JPH09131190A (ja) 1997-05-20
JPS6091986A (ja) 1985-05-23
BG49718A3 (bg) 1992-01-15
IL72396A0 (en) 1984-11-30
IL72396A (en) 1992-05-25
JPH0799990A (ja) 1995-04-18
HK128095A (en) 1995-08-18
EP0131843B1 (de) 1991-09-04
DD240217A5 (de) 1986-10-22
SK278576B6 (en) 1997-10-08
DK347184D0 (da) 1984-07-13
IE922469A1 (en) 1985-01-15
NZ227962A (en) 1990-06-26
DE3486425T2 (de) 1996-10-17
AU577298B2 (en) 1988-09-22
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IL91828A0 (en) 1990-06-10
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US4997916A (en) 1991-03-05
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