ATE93896T1 - Nachweis und bestimmung der menge an rns und dns. - Google Patents
Nachweis und bestimmung der menge an rns und dns.Info
- Publication number
- ATE93896T1 ATE93896T1 AT90900191T AT90900191T ATE93896T1 AT E93896 T1 ATE93896 T1 AT E93896T1 AT 90900191 T AT90900191 T AT 90900191T AT 90900191 T AT90900191 T AT 90900191T AT E93896 T1 ATE93896 T1 AT E93896T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- rna
- magnetic particles
- single stranded
- analyte
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 7
- 239000012491 analyte Substances 0.000 abstract 3
- 239000006249 magnetic particle Substances 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020003215 DNA Probes Proteins 0.000 abstract 1
- 239000003298 DNA probe Substances 0.000 abstract 1
- 102100034343 Integrase Human genes 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 230000004931 aggregating effect Effects 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000005546 dideoxynucleotide Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB888827160A GB8827160D0 (en) | 1988-11-21 | 1988-11-21 | Detection & quantitative determination of rna & dna |
| PCT/EP1989/001416 WO1990006042A2 (en) | 1988-11-21 | 1989-11-21 | Detection and quantitative determination of rna and dna |
| EP90900191A EP0444120B1 (de) | 1988-11-21 | 1989-11-21 | Nachweis und bestimmung der menge an rns und dns |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE93896T1 true ATE93896T1 (de) | 1993-09-15 |
Family
ID=10647188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT90900191T ATE93896T1 (de) | 1988-11-21 | 1989-11-21 | Nachweis und bestimmung der menge an rns und dns. |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0444120B1 (de) |
| JP (1) | JPH04501956A (de) |
| AT (1) | ATE93896T1 (de) |
| AU (1) | AU634993B2 (de) |
| CA (1) | CA2003501A1 (de) |
| DE (1) | DE68908900T2 (de) |
| GB (1) | GB8827160D0 (de) |
| WO (1) | WO1990006042A2 (de) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE66572B1 (en) * | 1989-02-13 | 1996-01-24 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| US5856092A (en) * | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| DK0463144T3 (da) * | 1989-12-14 | 1997-03-10 | Dade Int Inc | På magnetisme reagerende fluorscerende polymerpartikler og anvendelse deraf |
| GB9003253D0 (en) * | 1990-02-13 | 1990-04-11 | Amersham Int Plc | Precipitating polymers |
| US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
| US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
| WO1993004199A2 (en) * | 1991-08-20 | 1993-03-04 | Scientific Generics Limited | Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids |
| GB9127415D0 (en) * | 1991-12-24 | 1992-02-19 | Swordfish Int Ltd | Solid support bound detection and diagnostic system |
| CA2126748A1 (en) * | 1991-12-24 | 1993-06-25 | Tepnel Medical Limited | Manipulating nucleic acid sequences |
| MX9300492A (es) * | 1992-01-29 | 1994-07-29 | Hitachi Chemical Co Ltd | Soporte inmovilizado de nucleotido y metodo para su produccion. |
| US5580971A (en) * | 1992-07-28 | 1996-12-03 | Hitachi Chemical Company, Ltd. | Fungal detection system based on rRNA probes |
| JPH07509361A (ja) * | 1992-07-28 | 1995-10-19 | 日立化成工業株式会社 | 遺伝子検出システム |
| US5591841A (en) * | 1993-01-14 | 1997-01-07 | Ji; Huamin | Rapid purification of circular DNA by triplex-mediated affinity capture |
| ES2055661B1 (es) * | 1993-01-20 | 1995-03-01 | Univ Malaga | Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre. |
| US5591580A (en) * | 1994-03-31 | 1997-01-07 | Johnson & Johnson Clinical Diagnostics, Inc. | Method, test element and test kit for semi-quantitative detection of target nucleic acid |
| GB9408607D0 (en) * | 1994-04-29 | 1994-06-22 | Dynal As | Assay |
| AU707391B2 (en) * | 1994-05-28 | 1999-07-08 | Tepnel Medical Limited | Producing copies of nucleic acids |
| US6060288A (en) * | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
| US6090592A (en) * | 1994-08-03 | 2000-07-18 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid on supports |
| US5641658A (en) * | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| US5656429A (en) * | 1994-10-03 | 1997-08-12 | Adelman; Lonnie W. | Polynucleotide and protein analysis method using magnetizable moieties |
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| DE19526431A1 (de) * | 1995-07-21 | 1997-01-23 | Boehringer Mannheim Gmbh | Verfahren und Reagenz zur spezifischen Bestimmung von mRNA |
| US6261797B1 (en) * | 1996-01-29 | 2001-07-17 | Stratagene | Primer-mediated polynucleotide synthesis and manipulation techniques |
| DE19608687A1 (de) | 1996-03-06 | 1997-09-11 | Retro Tech Gmbh | Verfahren und Test-Kit für den nichtradioaktiven, enzymatischen Nachweis von Reverser Transkriptase |
| AU2320597A (en) | 1996-03-19 | 1997-10-10 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
| US6203978B1 (en) * | 1996-05-31 | 2001-03-20 | Du Vergier Ltd. | Capture of single stranded nucleic acids |
| US6060246A (en) * | 1996-11-15 | 2000-05-09 | Avi Biopharma, Inc. | Reagent and method for isolation and detection of selected nucleic acid sequences |
| GB9624165D0 (en) | 1996-11-19 | 1997-01-08 | Amdex A S | Use of nucleic acids bound to carrier macromolecules |
| EP2327797B1 (de) | 1997-04-01 | 2015-11-25 | Illumina Cambridge Limited | Verfahren zur Vervielfältigung von Nukleinsäuren |
| US6534273B2 (en) | 1997-05-02 | 2003-03-18 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
| DK0975807T3 (da) | 1997-05-02 | 2007-01-29 | Gen Probe Inc | To-trins hybridisering og indfangning af et polynukleotid |
| US6265163B1 (en) | 1998-01-09 | 2001-07-24 | Lynx Therapeutics, Inc. | Solid phase selection of differentially expressed genes |
| US6180348B1 (en) * | 1998-04-20 | 2001-01-30 | Weihua Li | Method of isolating target specific oligonucleotide ligands |
| AU2012232988B2 (en) * | 1998-05-01 | 2014-09-25 | Gen-Probe Incorporated | System and method for incubating the contents of a reaction receptacle |
| DE69940647D1 (de) | 1998-05-01 | 2009-05-07 | Gen Probe Inc | Automatische diagnostische Analysevorrichtung |
| US5973138A (en) | 1998-10-30 | 1999-10-26 | Becton Dickinson And Company | Method for purification and manipulation of nucleic acids using paramagnetic particles |
| GB9904991D0 (en) * | 1999-03-05 | 1999-04-28 | Univ Nottingham | Genetic screening |
| WO2001011364A1 (en) * | 1999-08-09 | 2001-02-15 | Precision System Science Co., Ltd. | Method for automatic labeling using dispenser, method for automatically separating target substance, method for determining base sequence, and automatic dispensing system |
| JP2001147230A (ja) | 1999-11-19 | 2001-05-29 | Hitachi Software Eng Co Ltd | バイオチップ読取装置及び標識試薬 |
| AU7125401A (en) | 2000-05-19 | 2001-12-03 | David J Marshall | Materials and methods for detection of nucleic acids |
| JP4887530B2 (ja) * | 2000-08-21 | 2012-02-29 | 独立行政法人産業技術総合研究所 | 磁性微粒子、およびその製造方法 |
| DE60137649D1 (de) * | 2000-10-14 | 2009-03-26 | Eragen Biosciences Inc | Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen |
| AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| US7514212B2 (en) | 2003-01-17 | 2009-04-07 | Eragen Biosciences, Inc. | Nucleic acid amplification using non-standard bases |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| WO2004090153A2 (en) | 2003-04-01 | 2004-10-21 | Eragen Biosciences,Inc. | Polymerase inhibitor and method of using same |
| JP4735926B2 (ja) * | 2004-05-27 | 2011-07-27 | アイシン精機株式会社 | 核酸の高効率回収方法 |
| WO2006085668A1 (en) * | 2005-02-08 | 2006-08-17 | Fujifilm Corporation | Magnetic composite body, production method thereof, method for removing substance with mannose on its surface, and method for concentrating substance with mannose on its surface |
| JP4903449B2 (ja) * | 2005-02-08 | 2012-03-28 | 富士フイルム株式会社 | 磁性複合体およびその製造方法、マンノースを表面に有する物質の除去方法及びマンノースを表面に有する物質の濃縮方法 |
| US8084275B2 (en) * | 2005-02-08 | 2011-12-27 | Fujifilm Corporation | Magnetic composite body, production method thereof, method for removing substance with mannose on its surface, and method for concentrating substance with mannose on its surface |
| WO2006101913A2 (en) | 2005-03-18 | 2006-09-28 | Eragen Biosciences, Inc. | Methods for detecting multiple species and subspecies of neiserria |
| CA2919210C (en) | 2005-06-07 | 2019-03-05 | Luminex Corporation | Methods for detection and typing of nucleic acids |
| AT502549B1 (de) | 2005-10-07 | 2007-06-15 | Anagnostics Bioanalysis Gmbh | Vorrichtung zur analyse von flüssigen proben |
| EP2121983A2 (de) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Verfahren zur kennzeichnung von proben und sequenzierung mehrerer nukleotidmatrizen |
| US8728764B2 (en) | 2008-10-02 | 2014-05-20 | Illumina Cambridge Limited | Nucleic acid sample enrichment for sequencing applications |
| US8182994B2 (en) | 2009-09-15 | 2012-05-22 | Illumina Cambridge Limited | Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing |
| CA2778449C (en) | 2009-10-23 | 2017-06-20 | Luminex Corporation | Amplification primers with non-standard bases for increased reaction specificity |
| KR20130029128A (ko) | 2010-07-23 | 2013-03-21 | 베크만 컬터, 인코포레이티드 | 실시간 pcr용 용기 |
| JP6013335B2 (ja) | 2011-08-11 | 2016-10-25 | オリンパス株式会社 | 標的粒子の計数方法 |
| EP2752655A4 (de) | 2011-08-30 | 2015-06-17 | Olympus Corp | Verfahren zum nachweis von zielpartikeln |
| US8973736B2 (en) | 2011-11-07 | 2015-03-10 | Beckman Coulter, Inc. | Magnetic damping for specimen transport system |
| WO2013070755A2 (en) | 2011-11-07 | 2013-05-16 | Beckman Coulter, Inc. | Centrifuge system and workflow |
| US9910054B2 (en) | 2011-11-07 | 2018-03-06 | Beckman Coulter, Inc. | System and method for processing samples |
| EP3373015A1 (de) | 2011-11-07 | 2018-09-12 | Beckman Coulter Inc. | Aliquotierungssystem und arbeitsablauf |
| KR20140091033A (ko) | 2011-11-07 | 2014-07-18 | 베크만 컬터, 인코포레이티드 | 검체 컨테이너 검출 |
| CN104040352B (zh) | 2011-11-07 | 2016-09-07 | 贝克曼考尔特公司 | 机械臂 |
| JP5940644B2 (ja) | 2012-02-22 | 2016-06-29 | オリンパス株式会社 | 標的粒子の検出方法 |
| EP2829614A4 (de) | 2012-03-21 | 2016-03-16 | Olympus Corp | Verfahren zur detektion eines zielnucleinsäuremoleküls |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554088A (en) * | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| ZA877772B (en) * | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
| AU619170B2 (en) * | 1987-01-09 | 1992-01-23 | Abbott Laboratories | Diagnostic assays using nucleic acid probes |
| AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| CA1317535C (en) * | 1987-06-30 | 1993-05-11 | Nanibhushan Dattagupta | Assay of sequences using amplified genes |
| DE3721851A1 (de) * | 1987-07-02 | 1989-01-12 | Bayer Ag | Herstellung von diazo- und azoverbindungen |
| CA2004326A1 (en) * | 1988-12-23 | 1990-06-23 | Nanibushan Dattagupta | Assay of sequences using amplified genes |
-
1988
- 1988-11-21 GB GB888827160A patent/GB8827160D0/en active Pending
-
1989
- 1989-11-21 EP EP90900191A patent/EP0444120B1/de not_active Expired - Lifetime
- 1989-11-21 DE DE90900191T patent/DE68908900T2/de not_active Expired - Fee Related
- 1989-11-21 JP JP2501002A patent/JPH04501956A/ja active Pending
- 1989-11-21 AT AT90900191T patent/ATE93896T1/de not_active IP Right Cessation
- 1989-11-21 CA CA002003501A patent/CA2003501A1/en not_active Abandoned
- 1989-11-21 AU AU47596/90A patent/AU634993B2/en not_active Ceased
- 1989-11-21 WO PCT/EP1989/001416 patent/WO1990006042A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE68908900T2 (de) | 1994-01-20 |
| WO1990006042A3 (en) | 1990-10-18 |
| GB8827160D0 (en) | 1988-12-29 |
| EP0444120B1 (de) | 1993-09-01 |
| CA2003501A1 (en) | 1990-05-21 |
| EP0444120A1 (de) | 1991-09-04 |
| AU4759690A (en) | 1990-06-26 |
| AU634993B2 (en) | 1993-03-11 |
| WO1990006042A2 (en) | 1990-06-14 |
| JPH04501956A (ja) | 1992-04-09 |
| DE68908900D1 (de) | 1993-10-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |