AU2001255331A1 - Detection and amplification or RNA using target-mediated ligation of DNA by RNA ligase - Google Patents

Detection and amplification or RNA using target-mediated ligation of DNA by RNA ligase

Info

Publication number
AU2001255331A1
AU2001255331A1 AU2001255331A AU5533101A AU2001255331A1 AU 2001255331 A1 AU2001255331 A1 AU 2001255331A1 AU 2001255331 A AU2001255331 A AU 2001255331A AU 5533101 A AU5533101 A AU 5533101A AU 2001255331 A1 AU2001255331 A1 AU 2001255331A1
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AU
Australia
Prior art keywords
probe
target
probes
rna
ligation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2001255331A
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English (en)
Inventor
A. Fawad Faruqi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Staging Inc
Original Assignee
Molecular Staging Inc
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Filing date
Publication date
Application filed by Molecular Staging Inc filed Critical Molecular Staging Inc
Publication of AU2001255331A1 publication Critical patent/AU2001255331A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001255331A 2000-04-12 2001-04-12 Detection and amplification or RNA using target-mediated ligation of DNA by RNA ligase Abandoned AU2001255331A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09547757 2000-04-12
US09/547,757 US6368801B1 (en) 2000-04-12 2000-04-12 Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
PCT/US2001/011947 WO2001079420A2 (en) 2000-04-12 2001-04-12 Detection and amplification or rna using target-mediated ligation of dna by rna ligase

Publications (1)

Publication Number Publication Date
AU2001255331A1 true AU2001255331A1 (en) 2001-10-30

Family

ID=24186008

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001255331A Abandoned AU2001255331A1 (en) 2000-04-12 2001-04-12 Detection and amplification or RNA using target-mediated ligation of DNA by RNA ligase

Country Status (8)

Country Link
US (1) US6368801B1 (de)
EP (1) EP1311703B1 (de)
JP (1) JP2003534782A (de)
AT (1) ATE396280T1 (de)
AU (1) AU2001255331A1 (de)
CA (1) CA2405456A1 (de)
DE (1) DE60134146D1 (de)
WO (1) WO2001079420A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7955795B2 (en) 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production

Families Citing this family (209)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
US8137911B2 (en) * 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US20040058330A1 (en) * 2002-09-20 2004-03-25 Prokaria, Ltd. Methods of use for thermostable RNA ligases
US7413857B2 (en) 2002-11-21 2008-08-19 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7192703B2 (en) 2003-02-14 2007-03-20 Intel Corporation, Inc. Biomolecule analysis by rolling circle amplification and SERS detection
US20040259128A1 (en) * 2003-03-24 2004-12-23 Glenn Kawasaki Methods and compositions for detecting the presence of target nucleic acids in a sample
US8043834B2 (en) * 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
US7446202B2 (en) 2003-12-05 2008-11-04 Molecular Probes, Inc. Cyanine dye compounds
GB0422730D0 (en) * 2004-10-13 2004-11-17 Lingvitae As Method
US20070020644A1 (en) * 2005-01-26 2007-01-25 Kolykhalov Alexander A Method for detection and characterization of short nucleic acids
WO2006091813A2 (en) * 2005-02-25 2006-08-31 Stowers Institute For Medical Research Novel thermophilic proteins and the nucleic acids encoding them
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
JP5306811B2 (ja) 2005-05-11 2013-10-02 ライフ テクノロジーズ コーポレーション 2本鎖dnaへの高い選択性を有する蛍光化学物質及びそれらの使用
US20080008997A1 (en) * 2005-08-30 2008-01-10 Board Of Regents, The University Of Texas System Proximity ligation assays with peptide conjugate 'burrs' and aptamers for the sensitive detection of spores and cancer cells
US20080293051A1 (en) * 2005-08-30 2008-11-27 Board Of Regents, The University Of Texas System proximity ligation assay
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
LT2336315T (lt) * 2005-12-01 2017-11-10 Nuevolution A/S Fermentiniai kodavimo būdai, skirti efektyviai didelių bibliotekų sintezei
US8383370B2 (en) * 2007-01-30 2013-02-26 The Rockefeller University Modified RNA ligase for efficient 3′ modification of RNA
CN101329250B (zh) * 2007-06-20 2011-11-30 上海翼和应用生物技术有限公司 一种荧光标记探针的pcr-ldr基因多态性检测测序方法
SI2215272T1 (sl) * 2007-10-31 2011-08-31 Debiopharm Sa Test skoraj popolnega genoma na odpornost proti hcv zdravilom
US20100034445A1 (en) * 2008-08-06 2010-02-11 Genome Corporation Continuous imaging of nucleic acids
US20100035325A1 (en) * 2008-08-06 2010-02-11 Genome Corporation Microcapsules and methods of use for amplification and sequencing
US20100035323A1 (en) * 2008-08-06 2010-02-11 Genome Corporation Methods of using a multiple sheath flow device for the production of microcapsules
US20100032295A1 (en) * 2008-08-06 2010-02-11 Genome Corporation Continuous film electrophoresis
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
WO2012021749A1 (en) * 2010-08-11 2012-02-16 Celula, Inc. Genotyping dna
ES3005233T3 (en) 2010-10-01 2025-03-14 Modernatx Inc Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
ES2632768T3 (es) 2011-01-17 2017-09-15 Life Technologies Corporation Flujo de trabajo para la detección de ligandos empleando ácidos nucleicos
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP2570487A1 (de) 2011-09-16 2013-03-20 Lexogen GmbH Verfahren zur Nukleinsäuretranskription
PL2756098T3 (pl) 2011-09-16 2018-12-31 Lexogen Gmbh Sposób wytwarzania biblioteki cząsteczek kwasów nukleinowych
EP2763701B1 (de) 2011-10-03 2018-12-19 Moderna Therapeutics, Inc. Modifizierte nukleoside, nukleotide und nukleinsäuren und verwendungen davon
US20130157259A1 (en) * 2011-12-15 2013-06-20 Samsung Electronics Co., Ltd. Method of amplifying dna from rna in sample and use thereof
JP2015501844A (ja) 2011-12-16 2015-01-19 モデルナ セラピューティクス インコーポレイテッドModerna Therapeutics,Inc. 修飾ヌクレオシド、ヌクレオチドおよび核酸組成物
WO2013096839A1 (en) * 2011-12-22 2013-06-27 Somagenics, Inc. Methods of constructing small rna libraries and their use for expression profiling of target rnas
US9121061B2 (en) 2012-03-15 2015-09-01 New England Biolabs, Inc. Methods and compositions for discrimination between cytosine and modifications thereof and for methylome analysis
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
WO2013151663A1 (en) 2012-04-02 2013-10-10 modeRNA Therapeutics Modified polynucleotides for the production of membrane proteins
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2653559A1 (de) * 2012-04-20 2013-10-23 Samsung Electronics Co., Ltd Polynucleotide und Verwendung davon
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
ES2921623T3 (es) 2012-11-26 2022-08-30 Modernatx Inc ARN modificado terminalmente
US10597650B2 (en) * 2012-12-21 2020-03-24 New England Biolabs, Inc. Ligase activity
US10597710B2 (en) 2012-12-21 2020-03-24 New England Biolabs, Inc. Ligase activity
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
US9618510B2 (en) 2013-03-13 2017-04-11 Meso Scale Technologies, Llc. Assay methods
BR112015023775B1 (pt) 2013-03-15 2022-08-16 Lubrizol Advanced Materials, Inc Composições de cpvc isentas de metal pesado
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
CN103160594B (zh) * 2013-04-03 2015-03-04 中国科学院苏州纳米技术与纳米仿生研究所 基于疏水芯片的多重pcr方法
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
US20160194625A1 (en) 2013-09-03 2016-07-07 Moderna Therapeutics, Inc. Chimeric polynucleotides
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
KR20160067219A (ko) 2013-10-03 2016-06-13 모더나 세라퓨틱스, 인코포레이티드 저밀도 지단백질 수용체를 암호화하는 폴리뉴클레오타이드
US10408823B2 (en) 2014-05-15 2019-09-10 Meso Scale Technologies, Llc. Assay methods
EP3169693B1 (de) 2014-07-16 2022-03-09 ModernaTX, Inc. Chimäre polynukleotide
US10590472B2 (en) 2014-09-05 2020-03-17 Zhi Zheng Methods of detecting nucleic acids and applications thereof
US10683534B2 (en) 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US11091810B2 (en) * 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US9856521B2 (en) * 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US20160108458A1 (en) * 2014-10-06 2016-04-21 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed detection and quantification of nucleic acids in single-cells
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
EP3298170B1 (de) 2015-05-21 2021-12-29 Cofactor Genomics, Inc. Verfahren zur erzeugung von zirkulärer dna aus zirkulärer rna
EP3394292B1 (de) 2015-12-21 2021-04-28 RealSeq Biosciences, Inc. Verfahren zur bibliothekskonstruktion zur sequenzierung von polynukleotiden
EP3420110B1 (de) 2016-02-26 2021-09-22 The Board of Trustees of the Leland Stanford Junior University Multiplexierte einzelmolekül-rna-visualisierung mit einem zwei-sonden-proximitätsligationssystem
EP3793715A2 (de) 2018-05-17 2021-03-24 Meso Scale Technologies, LLC Verfahren zur isolierung von oberflächenmarkerindikatoren
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020086751A1 (en) 2018-10-23 2020-04-30 Meso Scale Technologies, Llc. Methods for isolating surface marker displaying agents
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US20220290217A1 (en) 2018-12-10 2022-09-15 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
KR20260021093A (ko) 2019-03-01 2026-02-12 메소 스케일 테크놀러지즈, 엘엘시 면역검정 방법에서 사용을 위한 전기화학발광성 표지화된 프로브, 이의 사용 방법 및 이를 포함하는 키트
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
US20230109070A1 (en) 2019-10-18 2023-04-06 The Board of Trustees og the Leland Standford Junior University Clinical- and industrial-scale intact-tissue sequencing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4055185A1 (de) 2019-11-08 2022-09-14 10X Genomics, Inc. Räumlich markierte analytfänger für analyt-multiplexing
EP4081777B1 (de) 2019-12-23 2026-02-18 10X Genomics, Inc. Reversible fixierreagenzien und verfahren zu deren verwendung
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
EP4484571A3 (de) 2020-02-21 2025-03-19 10X Genomics, Inc. Verfahren und zusammensetzungen für integrierten räumlichen in-situ-assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
US20230125232A1 (en) * 2020-03-31 2023-04-27 Arcticzymes As Atp-dependent dna ligase
CZ308956B6 (cs) * 2020-04-14 2021-10-06 Ústav molekulární genetiky AV ČR, v.v.i. Způsob detekce RNA se zvýšenou účinností a citlivostí vhodný pro kvantitativní stanovení mRNA a reakční pufr pro použití při tomto způsobu
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US20240018571A1 (en) 2020-05-19 2024-01-18 Meso Scale Technologies, Llc. Methods, compositions, and kits for nucleic acid detection
CN116134308B (zh) 2020-05-19 2025-05-27 10X基因组学有限公司 电泳盒和仪器
EP4414459B1 (de) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4158054B1 (de) 2020-06-02 2025-04-16 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
EP4446430A3 (de) 2020-06-10 2024-12-18 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022051485A2 (en) 2020-09-02 2022-03-10 Meso Scale Technologies,Llc. Kits for detecting one or more target analytes in a sample and methods of making and using the same
EP4208720A2 (de) 2020-09-04 2023-07-12 Meso Scale Technologies, LLC Verfahren zur isolierung von markern für die oberfläche des zentralen nervensystems
WO2022061150A2 (en) 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4121555B1 (de) 2020-12-21 2026-02-25 10X Genomics, Inc. Verfahren zur erfassung von sonden und/oder barcodes
US20220195498A1 (en) 2020-12-23 2022-06-23 10X Genomics, Inc. Methods and compositions for analyte detection
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (de) 2021-01-26 2024-10-23 10X Genomics, Inc. Nukleinsäureanalogsonden zur in-situ-analyse
EP4421491A3 (de) 2021-02-19 2024-11-27 10X Genomics, Inc. Verfahren zur verwendung einer modularen testträgervorrichtung
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4301873A1 (de) 2021-03-03 2024-01-10 10X Genomics, Inc. In-situ-analytnachweis mit nukleinsäureorigami
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
EP4428246B1 (de) 2021-04-14 2025-12-24 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
CA3216872A1 (en) 2021-04-16 2022-10-20 Meso Scale Technologies, Llc. Methods, compositions, and kits for detecting hydrolase enzyme activity
US20240210399A1 (en) 2021-04-26 2024-06-27 Meso Scale Technologies, Llc. Methods for isolating and analyzing a target analyte encapsulated by a surface marker displaying agent
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
WO2022241227A1 (en) 2021-05-14 2022-11-17 Akoya Biosciences, Inc. Amplification of rna detection signals in biological samples
EP4347877A1 (de) 2021-06-01 2024-04-10 10X Genomics, Inc. Verfahren und zusammensetzungen zum nachweis von analyten und zur sondenauflösung
EP4347880B1 (de) 2021-06-02 2026-02-04 10X Genomics, Inc. Probenanalyse mit asymmetrischen zirkularisierbaren sonden
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023283442A1 (en) 2021-07-09 2023-01-12 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
WO2023023484A1 (en) 2021-08-16 2023-02-23 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
EP4196605B1 (de) 2021-09-01 2024-12-04 10X Genomics, Inc. Verfahren zur blockierung einer erfassungssonde auf einer räumlichen anordnung
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4445377A2 (de) 2021-12-10 2024-10-16 10X Genomics, Inc. In-situ-decodierung mit mehreren auflösungen
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
WO2023141588A1 (en) 2022-01-21 2023-07-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
AU2023223467A1 (en) 2022-02-23 2024-08-08 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
WO2023172915A1 (en) 2022-03-08 2023-09-14 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
WO2023192302A1 (en) 2022-03-29 2023-10-05 10X Genomics, Inc. Spectral unmixing combined with decoding for super-multiplexed in situ analysis
EP4504969A1 (de) 2022-04-06 2025-02-12 10X Genomics, Inc. Verfahren zur multiplex-zellanalyse
EP4519674A1 (de) 2022-05-06 2025-03-12 10X Genomics, Inc. Analyse von antigen- und antigenrezeptorinteraktionen
WO2023220300A1 (en) 2022-05-11 2023-11-16 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (de) 2022-06-17 2025-04-23 10X Genomics, Inc. Katalytische entvernetzung von proben zur in-situ-analyse
CN120129825A (zh) 2022-10-14 2025-06-10 10X基因组学有限公司 用于评估生物样品质量的方法、组合物和系统
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
EP4573210B1 (de) 2022-11-16 2026-05-06 10X Genomics, Inc. Verfahren zur analyse der genexprimierung in zellen in einem pellet
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
WO2024229260A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
EP4720676A1 (de) 2023-05-24 2026-04-08 Meso Scale Technologies, LLC Verfahren zur beurteilung von kolorektalkarzinom mit extrazellulären vesikeln
WO2025014758A1 (en) 2023-07-07 2025-01-16 10X Genomics, Inc. Systems and methods for tissue bounds detection
US12417646B2 (en) 2023-08-16 2025-09-16 10X Genomics, Inc. Systems and methods for image segmentation
WO2025076421A1 (en) 2023-10-06 2025-04-10 10X Genomics, Inc. Feature pyramiding for in situ data visualizations
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
WO2025122890A1 (en) 2023-12-07 2025-06-12 10X Genomics, Inc. Graphical user interface and method of estimating an instrument run completion time
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025160489A1 (en) 2024-01-25 2025-07-31 10X Genomics, Inc. Systems and methods for image segmentation using multiple stain indicators
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging
US20250285229A1 (en) 2024-03-08 2025-09-11 10X Genomics, Inc. Multi-focus image fusion with background removal
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2025264702A1 (en) 2024-06-18 2025-12-26 10X Genomics, Inc. 3d hydrogels for nucleic acid sequencing
WO2026055487A1 (en) 2024-09-06 2026-03-12 10X Genomics, Inc. Modified nucleotides with oligomer spacers
WO2026076066A1 (en) 2024-10-01 2026-04-09 10X Genomics, Inc. Systems and methods for illumination of a sample
WO2026097020A1 (en) 2024-11-04 2026-05-07 10X Genomics, Inc. Polymerase for sequencing by synthesis

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
DE3929144A1 (de) 1989-09-02 1991-03-07 Behringwerke Ag Nachweis von influenza-a-virus durch polymerase-kettenreaktion (pcr) nach reverser transkription eines bereichs des viralen haemagglutinin-gens
US5516663A (en) 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
CA2046713A1 (en) 1990-10-16 1992-04-17 Richard M. Martinelli Amplification of midivariant dna templates
EP0682715B1 (de) 1993-01-15 2006-04-05 The Public Health Research Institute of the City of New York, Inc. Diagnostischer nachweis von rna und reagentiensätze unter verwendung binärer rna proben und einer rna-abhängigen rna ligase
US5871914A (en) 1993-06-03 1999-02-16 Intelligene Ltd. Method for detecting a nucleic acid involving the production of a triggering RNA and transcription amplification
US5429807A (en) 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
DE69506393T2 (de) 1994-04-04 1999-07-01 Ciba Corning Diagnostics Corp., Medfield, Mass. Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
CA2195562A1 (en) 1994-08-19 1996-02-29 Pe Corporation (Ny) Coupled amplification and ligation method
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
JP2809601B2 (ja) 1995-07-13 1998-10-15 株式会社分子バイオホトニクス研究所 塩基配列増幅方法
US5888731A (en) 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ATE238434T1 (de) 1995-12-05 2003-05-15 Jorn Erland Koch Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5998175A (en) 1998-07-24 1999-12-07 Lumigen, Inc. Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US6297016B1 (en) * 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7955795B2 (en) 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production

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