AU2004227366B2 - Methods for neural differentiation of embryonic stem cells using protease passaging techniques - Google Patents

Methods for neural differentiation of embryonic stem cells using protease passaging techniques Download PDF

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Publication number
AU2004227366B2
AU2004227366B2 AU2004227366A AU2004227366A AU2004227366B2 AU 2004227366 B2 AU2004227366 B2 AU 2004227366B2 AU 2004227366 A AU2004227366 A AU 2004227366A AU 2004227366 A AU2004227366 A AU 2004227366A AU 2004227366 B2 AU2004227366 B2 AU 2004227366B2
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Australia
Prior art keywords
cells
cell
neural
culture
differentiation
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Ceased
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AU2004227366A
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AU2004227366A1 (en
Inventor
Brian Condie
Allan Robins
Thomas C. Schulz
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University of Georgia Research Foundation Inc
Viacyte Georgia Inc
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University of Georgia
University of Georgia Research Foundation Inc
Viacyte Georgia Inc
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Priority to AU2009201414A priority Critical patent/AU2009201414A1/en
Assigned to VIACYTE GEORGIA, INC., UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. reassignment VIACYTE GEORGIA, INC. Request to Amend Deed and Register Assignors: BRESAGEN, INC., UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2004227366A 2002-08-08 2004-03-31 Methods for neural differentiation of embryonic stem cells using protease passaging techniques Ceased AU2004227366B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2009201414A AU2009201414A1 (en) 2002-08-08 2009-04-09 Methods for neural differentiation of embryonic stem cells using protease passaging techniques

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40196802P 2002-08-08 2002-08-08
US45909003P 2003-03-31 2003-03-31
US60/459,090 2003-03-31
PCT/US2004/010121 WO2004090096A2 (fr) 2002-08-08 2004-03-31 Procedes de differenciation neuronale de cellules souches embryonnaires au moyen de techniques de passage de protease

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2009201414A Division AU2009201414A1 (en) 2002-08-08 2009-04-09 Methods for neural differentiation of embryonic stem cells using protease passaging techniques

Publications (2)

Publication Number Publication Date
AU2004227366A1 AU2004227366A1 (en) 2004-10-21
AU2004227366B2 true AU2004227366B2 (en) 2009-01-08

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
AU2004227366A Ceased AU2004227366B2 (en) 2002-08-08 2004-03-31 Methods for neural differentiation of embryonic stem cells using protease passaging techniques
AU2009201414A Abandoned AU2009201414A1 (en) 2002-08-08 2009-04-09 Methods for neural differentiation of embryonic stem cells using protease passaging techniques

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2009201414A Abandoned AU2009201414A1 (en) 2002-08-08 2009-04-09 Methods for neural differentiation of embryonic stem cells using protease passaging techniques

Country Status (5)

Country Link
US (1) US20060121607A1 (fr)
EP (2) EP1534068A4 (fr)
AU (2) AU2004227366B2 (fr)
CA (1) CA2521039A1 (fr)
WO (2) WO2004015077A2 (fr)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7927587B2 (en) 1999-08-05 2011-04-19 Regents Of The University Of Minnesota MAPC administration for the treatment of lysosomal storage disorders
US7588937B2 (en) 2001-10-03 2009-09-15 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US8153424B2 (en) 2001-10-03 2012-04-10 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
BRPI0408733A (pt) 2003-03-12 2006-03-07 Reliance Life Sciences Pvt Ltd derivação de neurÈnios dopaminérgicos terminalmente diferenciados de células tronco embriÈnicas humanas
US7820439B2 (en) 2003-09-03 2010-10-26 Reliance Life Sciences Pvt Ltd. In vitro generation of GABAergic neurons from pluripotent stem cells
JP4676442B2 (ja) * 2003-12-02 2011-04-27 セラヴィー バイオサイエンシズ エルエルシー 神経前駆細胞を増殖させるための組成物および方法
EP1694345A1 (fr) * 2003-12-04 2006-08-30 Regents Of The University Of Minnesota Compositions et methodes de traitement des maladies lysosomales
CA2563087A1 (fr) * 2004-04-09 2005-12-15 Ronald S. Goldstein Methodes de production de cellules neuronales a partir de cellules souches embryonnaires humaines et leurs applications
WO2005123902A1 (fr) * 2004-06-18 2005-12-29 Riken Méthode pour induire la différenciation de cellules souches embryonnaires en nerf à l'aide d'une culture en suspension exempte de sérum
WO2006084314A1 (fr) * 2005-02-09 2006-08-17 Australian Stem Cell Centre Limited Populations de cellules souches et systeme de classification
SG170021A1 (en) 2006-02-23 2011-04-29 Novocell Inc Compositions and methods useful for culturing differentiable cells
US20100135970A1 (en) * 2006-10-27 2010-06-03 Caritas St. Elizabeth Medical Center Of Boston, In Methods for Reprogramming Adult Somatic Cells and Uses Thereof
WO2008063675A2 (fr) 2006-11-24 2008-05-29 Regents Of The University Of Minnesota Cellules progénitrices endothermiques
WO2008075377A2 (fr) * 2006-12-19 2008-06-26 Stempeutics Research Private Limited Cellules nourricières dérivées de lignées de germes et méthodes associées
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
DK2694644T3 (en) 2011-03-30 2018-04-16 Cellular Dynamics Int Inc Priming of pluripotent stem cells for neural differentiation
KR101793722B1 (ko) * 2014-08-25 2017-11-03 한국생명공학연구원 성상세포의 생산방법
WO2016032151A1 (fr) * 2014-08-25 2016-03-03 한국생명공학연구원 Procédé de production de cellules neuronales cholinergiques
US11566221B2 (en) 2016-10-24 2023-01-31 Life Technologies Corporation Methods of differentiation to neuronal cells and kits therefor
US20190167724A1 (en) * 2017-12-04 2019-06-06 Caire Medical-Biotechnology International Co. Neuroprotective composition, preparation process thereof and medical uses thereof
KR102062291B1 (ko) 2018-04-23 2020-01-03 주식회사 엔지켐생명과학 글리세롤 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 면역조절제
CN111484977B (zh) * 2019-01-25 2023-05-16 中国科学院脑科学与智能技术卓越创新中心 重编程产生功能性去甲肾上腺素能神经元的方法
CN114657119B (zh) * 2022-03-16 2023-10-20 广东省农业科学院动物科学研究所 一种初生仔猪肠神经胶质细胞的分离和原代培养方法
WO2025100546A1 (fr) * 2023-11-10 2025-05-15 株式会社幹細胞＆デバイス研究所 Procédé de production de cellules progénitrices de schwann et procédé de production de cellules de schwann
KR20250165009A (ko) * 2024-05-17 2025-11-25 대봉엘에스 주식회사 엔도카나비노이드 유사 세라마이드 화합물, 및 그의 용도

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6280718B1 (en) * 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
US20020016794A1 (en) * 2000-03-09 2002-02-07 The Web Access, Inc. Method and apparatus for accessing data within an electronic system by an external system
US20020160510A1 (en) * 2001-02-14 2002-10-31 Hariri Robert J. Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells
US20030166272A1 (en) * 1995-02-02 2003-09-04 Abuljadayel Ilham Saleh Method of preparing an undifferentiated cell

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851832A (en) * 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny
US5958829A (en) * 1992-02-14 1999-09-28 Degussa-Huls Aktiengesellschaft Coating dispersion for exhaust gas catalysts
US5589376A (en) * 1992-07-27 1996-12-31 California Institute Of Technology Mammalian neural crest stem cells
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5766948A (en) * 1993-01-06 1998-06-16 The Regents Of The University Of California Method for production of neuroblasts
US5753506A (en) * 1996-05-23 1998-05-19 Cns Stem Cell Technology, Inc. Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals
US6331406B1 (en) * 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
DE19756864C5 (de) * 1997-12-19 2014-07-10 Oliver Brüstle Neurale Vorläuferzellen, Verfahren zu ihrer Herstellung und ihre Verwendung zur Therapie von neuralen Defekten
WO2001083715A2 (fr) * 2000-05-01 2001-11-08 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the Secretary, Derivation de neurones dopaminergiques du mesencephale de cellules souches embryonnaires
JP2004504834A (ja) * 2000-08-01 2004-02-19 イスム　リサーチ　ディベロップメント　カンパニー 胚性細胞の定方向分化
WO2002026941A2 (fr) * 2000-09-29 2002-04-04 Kooy Derek V D Cellules nerveuses embryonnaires primitives et procede de differenciation de cellules embryonnaires par rapport a des cellules nerveuses
WO2003078585A2 (fr) * 2002-03-13 2003-09-25 University Of Georgia Research Foundation, Inc. Compositions et procedes de production de cellules neurales de primate
AUPS225402A0 (en) * 2002-05-10 2002-06-13 Bresagen Limited Modified cell production

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6200806B1 (en) * 1995-01-20 2001-03-13 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US20030166272A1 (en) * 1995-02-02 2003-09-04 Abuljadayel Ilham Saleh Method of preparing an undifferentiated cell
US6280718B1 (en) * 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
US20020016794A1 (en) * 2000-03-09 2002-02-07 The Web Access, Inc. Method and apparatus for accessing data within an electronic system by an external system
US20020160510A1 (en) * 2001-02-14 2002-10-31 Hariri Robert J. Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells

Also Published As

Publication number Publication date
WO2004015077A2 (fr) 2004-02-19
WO2004015077A9 (fr) 2004-06-17
WO2004090096A2 (fr) 2004-10-21
WO2004015077A3 (fr) 2004-05-13
EP1615997A2 (fr) 2006-01-18
CA2521039A1 (fr) 2004-10-21
WO2004090096A3 (fr) 2005-03-03
EP1534068A4 (fr) 2006-08-23
EP1534068A2 (fr) 2005-06-01
US20060121607A1 (en) 2006-06-08
AU2009201414A1 (en) 2009-05-07
EP1615997A4 (fr) 2007-08-29
AU2004227366A1 (en) 2004-10-21

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