AU2013276084B2 - Method for detecting and identifying enterohemorrhagic Escherichia coli - Google Patents

Method for detecting and identifying enterohemorrhagic Escherichia coli Download PDF

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AU2013276084B2
AU2013276084B2 AU2013276084A AU2013276084A AU2013276084B2 AU 2013276084 B2 AU2013276084 B2 AU 2013276084B2 AU 2013276084 A AU2013276084 A AU 2013276084A AU 2013276084 A AU2013276084 A AU 2013276084A AU 2013276084 B2 AU2013276084 B2 AU 2013276084B2
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ehec
primers
seq
crispr
strains
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AU2013276084A8 (en
AU2013276084A1 (en
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Lothar Beutin
Sabine Delannoy
Patrick Fach
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Agence National de Securite Sanitaire de l'Alimentation de l'Environnement Et du Travail
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2013276084A 2012-06-14 2013-06-14 Method for detecting and identifying enterohemorrhagic Escherichia coli Active AU2013276084B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12171941.3A EP2674501A1 (en) 2012-06-14 2012-06-14 Method for detecting and identifying enterohemorrhagic Escherichia coli
EP12171941.3 2012-06-14
PCT/IB2013/054888 WO2013186754A2 (en) 2012-06-14 2013-06-14 Method for detecting and identifying enterohemorrhagic escherichia coli

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AU2013276084A1 AU2013276084A1 (en) 2015-01-29
AU2013276084A8 AU2013276084A8 (en) 2015-02-12
AU2013276084B2 true AU2013276084B2 (en) 2019-04-04

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US (1) US10190175B2 (2)
EP (2) EP2674501A1 (2)
JP (1) JP6166365B2 (2)
KR (1) KR102065053B1 (2)
CN (1) CN104619860B (2)
AU (1) AU2013276084B2 (2)
BR (1) BR112014031370B1 (2)
CA (1) CA2875919C (2)
CL (1) CL2014003377A1 (2)
ES (1) ES2659469T3 (2)
MX (1) MX357697B (2)
WO (1) WO2013186754A2 (2)

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CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
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KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
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Also Published As

Publication number Publication date
CA2875919A1 (en) 2013-12-19
KR102065053B1 (ko) 2020-01-10
JP6166365B2 (ja) 2017-07-19
CA2875919C (en) 2021-08-31
WO2013186754A3 (en) 2014-02-20
MX357697B (es) 2018-07-19
WO2013186754A2 (en) 2013-12-19
CN104619860B (zh) 2018-01-30
BR112014031370A2 (pt) 2017-07-25
US10190175B2 (en) 2019-01-29
CL2014003377A1 (es) 2015-08-07
EP2674501A1 (en) 2013-12-18
MX2014015130A (es) 2015-08-06
ES2659469T3 (es) 2018-03-15
AU2013276084A8 (en) 2015-02-12
EP2861762A2 (en) 2015-04-22
CN104619860A (zh) 2015-05-13
KR20150030783A (ko) 2015-03-20
US20150176064A1 (en) 2015-06-25
JP2015519075A (ja) 2015-07-09
EP2861762B1 (en) 2017-12-13
AU2013276084A1 (en) 2015-01-29
BR112014031370B1 (pt) 2022-06-07

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