ES2659469T3 - Método para detectar e identificar Escherichia Coli enterohemorrágica - Google Patents
Método para detectar e identificar Escherichia Coli enterohemorrágica Download PDFInfo
- Publication number
- ES2659469T3 ES2659469T3 ES13747516.6T ES13747516T ES2659469T3 ES 2659469 T3 ES2659469 T3 ES 2659469T3 ES 13747516 T ES13747516 T ES 13747516T ES 2659469 T3 ES2659469 T3 ES 2659469T3
- Authority
- ES
- Spain
- Prior art keywords
- eceh
- primers
- seq
- crispr
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000588724 Escherichia coli Species 0.000 title claims abstract description 125
- 238000000034 method Methods 0.000 title claims abstract description 41
- 108091033409 CRISPR Proteins 0.000 claims abstract description 136
- 238000010354 CRISPR gene editing Methods 0.000 claims abstract description 135
- 108020004414 DNA Proteins 0.000 claims abstract description 19
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims abstract description 7
- 101150082166 espK gene Proteins 0.000 claims description 137
- 239000000523 sample Substances 0.000 claims description 94
- 230000003321 amplification Effects 0.000 claims description 85
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 85
- 238000001514 detection method Methods 0.000 claims description 74
- 101100021923 Helicobacter pylori (strain ATCC 700392 / 26695) lspA gene Proteins 0.000 claims description 48
- 101150070667 ureD gene Proteins 0.000 claims description 48
- 238000002944 PCR assay Methods 0.000 claims description 41
- 108090000623 proteins and genes Proteins 0.000 claims description 30
- 239000013615 primer Substances 0.000 description 111
- 230000002068 genetic effect Effects 0.000 description 59
- 230000001717 pathogenic effect Effects 0.000 description 26
- 108010079723 Shiga Toxin Proteins 0.000 description 24
- 101150107911 eae gene Proteins 0.000 description 21
- 230000035945 sensitivity Effects 0.000 description 19
- 101100500479 Hafnia alvei eaeA gene Proteins 0.000 description 18
- 238000003753 real-time PCR Methods 0.000 description 16
- 238000009826 distribution Methods 0.000 description 14
- 230000001018 virulence Effects 0.000 description 12
- -1 phages or plasmids Chemical class 0.000 description 11
- 230000037029 cross reaction Effects 0.000 description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 description 9
- 235000013305 food Nutrition 0.000 description 9
- 241000282412 Homo Species 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 208000015181 infectious disease Diseases 0.000 description 8
- 239000003550 marker Substances 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000036541 health Effects 0.000 description 6
- 239000002987 primer (paints) Substances 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 238000003556 assay Methods 0.000 description 5
- 208000032759 Hemolytic-Uremic Syndrome Diseases 0.000 description 4
- 125000006850 spacer group Chemical group 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 101100001161 Escherichia coli aggR gene Proteins 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000012636 effector Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000369 enteropathogenic effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 206010014896 Enterocolitis haemorrhagic Diseases 0.000 description 2
- 238000007397 LAMP assay Methods 0.000 description 2
- 101150000110 Nle gene Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 108010017898 Shiga Toxins Proteins 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 description 1
- COCMHKNAGZHBDZ-UHFFFAOYSA-N 4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate Chemical compound C=12C=CC(=[N+](C)C)C=C2OC2=CC(N(C)C)=CC=C2C=1C1=CC(C([O-])=O)=CC=C1C(O)=O COCMHKNAGZHBDZ-UHFFFAOYSA-N 0.000 description 1
- WQZIDRAQTRIQDX-UHFFFAOYSA-N 6-carboxy-x-rhodamine Chemical compound OC(=O)C1=CC=C(C([O-])=O)C=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 WQZIDRAQTRIQDX-UHFFFAOYSA-N 0.000 description 1
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 244000135860 Capparis spinosa subsp spinosa Species 0.000 description 1
- 101100516697 Citrobacter rodentium nleB gene Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000059459 Escherichia coli O104 Species 0.000 description 1
- 241001036088 Escherichia coli O104:H4 Species 0.000 description 1
- 101100240631 Escherichia coli O127:H6 (strain E2348/69 / EPEC) nleB1 gene Proteins 0.000 description 1
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 1
- 241000010756 Escherichia coli O157:H7 str. EDL933 Species 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000002547 anomalous effect Effects 0.000 description 1
- 101150117498 arcA gene Proteins 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 108010056602 colicin immunity proteins Proteins 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 101150080727 stx gene Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12171941.3A EP2674501A1 (en) | 2012-06-14 | 2012-06-14 | Method for detecting and identifying enterohemorrhagic Escherichia coli |
| EP12171941 | 2012-06-14 | ||
| PCT/IB2013/054888 WO2013186754A2 (en) | 2012-06-14 | 2013-06-14 | Method for detecting and identifying enterohemorrhagic escherichia coli |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2659469T3 true ES2659469T3 (es) | 2018-03-15 |
Family
ID=48949190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13747516.6T Active ES2659469T3 (es) | 2012-06-14 | 2013-06-14 | Método para detectar e identificar Escherichia Coli enterohemorrágica |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10190175B2 (2) |
| EP (2) | EP2674501A1 (2) |
| JP (1) | JP6166365B2 (2) |
| KR (1) | KR102065053B1 (2) |
| CN (1) | CN104619860B (2) |
| AU (1) | AU2013276084B2 (2) |
| BR (1) | BR112014031370B1 (2) |
| CA (1) | CA2875919C (2) |
| CL (1) | CL2014003377A1 (2) |
| ES (1) | ES2659469T3 (2) |
| MX (1) | MX357697B (2) |
| WO (1) | WO2013186754A2 (2) |
Families Citing this family (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| PL3360964T3 (pl) | 2012-12-06 | 2020-03-31 | Sigma-Aldrich Co. Llc | Modyfikacja i regulacja genomu oparta na crispr |
| RU2662932C2 (ru) | 2013-03-14 | 2018-07-31 | Карибо Биосайенсиз, Инк. | Композиции и способы с участием нуклеиновых кислот, нацеленных на нуклеиновые кислоты |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
| CN112301024A (zh) | 2013-03-15 | 2021-02-02 | 通用医疗公司 | 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性 |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| CN106459995B (zh) | 2013-11-07 | 2020-02-21 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| US10920285B2 (en) | 2014-12-03 | 2021-02-16 | Qiagen Gmbh | Highly specific and sensitive methods for detecting enterohemorrhagic Escherichia coli serotypes O157:H7 and/or O145:H28 |
| WO2016115441A1 (en) * | 2015-01-16 | 2016-07-21 | Kansas State University Research Foundation | Co-detection and association of multiple genes from the same genome in a sample |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| JP6799586B2 (ja) | 2015-08-28 | 2020-12-16 | ザ ジェネラル ホスピタル コーポレイション | 遺伝子操作CRISPR−Cas9ヌクレアーゼ |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| CN105779584A (zh) * | 2015-12-18 | 2016-07-20 | 珠海出入境检验检疫局检验检疫技术中心 | 大肠杆菌o111检测用的引物和探针组、试剂盒及pcr检测方法 |
| CN105821124A (zh) * | 2016-04-06 | 2016-08-03 | 山东省滨州畜牧兽医研究院 | 一种大肠杆菌 o26、o45、o121血清型三重pcr检测试剂盒及其引物组 |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11591589B2 (en) | 2017-04-21 | 2023-02-28 | The General Hospital Corporation | Variants of Cpf1 (Cas12a) with altered PAM specificity |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| EP3630849A4 (en) | 2017-05-25 | 2021-01-13 | The General Hospital Corporation | BIPARTITE BASIC EDITOR (BBE) AND TYPE II-C-CAS9 ZINC FINGER EDITOR ARCHITECTURES |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3731959B1 (en) * | 2017-12-29 | 2025-10-08 | Clear Labs, Inc. | Automated priming and library loading device |
| US10597714B2 (en) | 2017-12-29 | 2020-03-24 | Clear Labs, Inc. | Automated priming and library loading device |
| US10246704B1 (en) | 2017-12-29 | 2019-04-02 | Clear Labs, Inc. | Detection of microorganisms in food samples and food processing facilities |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| CN108950033B (zh) * | 2018-07-26 | 2022-01-11 | 江苏省农业科学院 | 同时检测肠出血性大肠杆菌o45和o145的纳米pcr试剂盒及其应用 |
| EP3847277B1 (en) * | 2018-09-06 | 2024-12-11 | Hygiena, LLC | Sequences and their use for detection and characterization of escherichia coli serotype o157:h7 |
| CN111100862B (zh) * | 2018-10-26 | 2023-09-29 | 厦门大学 | 一种检测细菌血清型的方法 |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| US11946040B2 (en) | 2019-02-04 | 2024-04-02 | The General Hospital Corporation | Adenine DNA base editor variants with reduced off-target RNA editing |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| US20230279442A1 (en) | 2021-12-15 | 2023-09-07 | Versitech Limited | Engineered cas9-nucleases and method of use thereof |
| CN116903710B (zh) * | 2023-09-12 | 2023-11-14 | 山东省农业科学院畜牧兽医研究所 | 噬菌体靶向蛋白分子及其在鉴定o103抗原血清型的产志贺毒素大肠杆菌中的应用 |
| WO2026006542A2 (en) | 2024-06-26 | 2026-01-02 | Yale University | Compositions and methods for crispr/cas9 based reactivation of human angelman syndrome |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002355074A (ja) * | 2001-01-24 | 2002-12-10 | Univ Tsukuba | 腸管出血性病原性大腸菌o157:h7に特異的な核酸分子およびポリペプチド並びにこれらの使用方法 |
| US20060051751A1 (en) * | 2002-01-23 | 2006-03-09 | Gehua Wang | Major virulence factor detection and verocytontoxin type 2 subtype from clinical e. coli isolates using a one-step multiplex pcr |
| EP2292799A1 (en) * | 2009-08-11 | 2011-03-09 | Agence Française de Securité Sanitaire des Aliments | An assay for determining a molecular risk assessment of stec isolates |
-
2012
- 2012-06-14 EP EP12171941.3A patent/EP2674501A1/en not_active Withdrawn
-
2013
- 2013-06-14 JP JP2015516741A patent/JP6166365B2/ja active Active
- 2013-06-14 CN CN201380030965.9A patent/CN104619860B/zh active Active
- 2013-06-14 US US14/407,884 patent/US10190175B2/en active Active
- 2013-06-14 EP EP13747516.6A patent/EP2861762B1/en active Active
- 2013-06-14 WO PCT/IB2013/054888 patent/WO2013186754A2/en not_active Ceased
- 2013-06-14 BR BR112014031370-9A patent/BR112014031370B1/pt active IP Right Grant
- 2013-06-14 AU AU2013276084A patent/AU2013276084B2/en active Active
- 2013-06-14 ES ES13747516.6T patent/ES2659469T3/es active Active
- 2013-06-14 MX MX2014015130A patent/MX357697B/es active IP Right Grant
- 2013-06-14 CA CA2875919A patent/CA2875919C/en active Active
- 2013-06-14 KR KR1020157000638A patent/KR102065053B1/ko active Active
-
2014
- 2014-12-12 CL CL2014003377A patent/CL2014003377A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2875919A1 (en) | 2013-12-19 |
| KR102065053B1 (ko) | 2020-01-10 |
| JP6166365B2 (ja) | 2017-07-19 |
| CA2875919C (en) | 2021-08-31 |
| WO2013186754A3 (en) | 2014-02-20 |
| MX357697B (es) | 2018-07-19 |
| WO2013186754A2 (en) | 2013-12-19 |
| CN104619860B (zh) | 2018-01-30 |
| BR112014031370A2 (pt) | 2017-07-25 |
| US10190175B2 (en) | 2019-01-29 |
| CL2014003377A1 (es) | 2015-08-07 |
| EP2674501A1 (en) | 2013-12-18 |
| MX2014015130A (es) | 2015-08-06 |
| AU2013276084A8 (en) | 2015-02-12 |
| EP2861762A2 (en) | 2015-04-22 |
| CN104619860A (zh) | 2015-05-13 |
| AU2013276084B2 (en) | 2019-04-04 |
| KR20150030783A (ko) | 2015-03-20 |
| US20150176064A1 (en) | 2015-06-25 |
| JP2015519075A (ja) | 2015-07-09 |
| EP2861762B1 (en) | 2017-12-13 |
| AU2013276084A1 (en) | 2015-01-29 |
| BR112014031370B1 (pt) | 2022-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2659469T3 (es) | Método para detectar e identificar Escherichia Coli enterohemorrágica | |
| US10724106B2 (en) | Method for determining the presence of diarrhoea causing pathogens | |
| You et al. | A novel DNA microarray for rapid diagnosis of enteropathogenic bacteria in stool specimens of patients with diarrhea | |
| ES2614811T3 (es) | Un ensayo para determinar una evaluación del riesgo molecular de una muestra polimicrobiana compleja sospechosa de contener un EHEC | |
| Michel et al. | Genetic profiles of Shiga toxin and intimin genes found in stool broth cultures: a 2-year reference laboratory study | |
| Moore et al. | Molecular characterisation of the rec A locus in clinical isolates of verocytotoxigenic E. coli O157: H7 | |
| Verstraete | Detection, isolation and typing of Shiga toxin producing (STEC) O26, O103, O111, O145, and O157 in cattle faeces, food and humans |