AU2014278537B2 - Methods, kits, and systems for multiplexed detection of target molecules and uses thereof - Google Patents
Methods, kits, and systems for multiplexed detection of target molecules and uses thereof Download PDFInfo
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- AU2014278537B2 AU2014278537B2 AU2014278537A AU2014278537A AU2014278537B2 AU 2014278537 B2 AU2014278537 B2 AU 2014278537B2 AU 2014278537 A AU2014278537 A AU 2014278537A AU 2014278537 A AU2014278537 A AU 2014278537A AU 2014278537 B2 AU2014278537 B2 AU 2014278537B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Kinetics & Catalysis (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2018204228A AU2018204228B2 (en) | 2013-06-12 | 2018-06-14 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
| AU2020204460A AU2020204460B2 (en) | 2013-06-12 | 2020-07-03 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361834111P | 2013-06-12 | 2013-06-12 | |
| US61/834,111 | 2013-06-12 | ||
| US201361912054P | 2013-12-05 | 2013-12-05 | |
| US61/912,054 | 2013-12-05 | ||
| US201461972940P | 2014-03-31 | 2014-03-31 | |
| US61/972,940 | 2014-03-31 | ||
| PCT/US2014/040731 WO2014200767A1 (en) | 2013-06-12 | 2014-06-03 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2018204228A Division AU2018204228B2 (en) | 2013-06-12 | 2018-06-14 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2014278537A1 AU2014278537A1 (en) | 2016-01-07 |
| AU2014278537B2 true AU2014278537B2 (en) | 2018-04-19 |
Family
ID=52022666
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2014278537A Active AU2014278537B2 (en) | 2013-06-12 | 2014-06-03 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
| AU2018204228A Active AU2018204228B2 (en) | 2013-06-12 | 2018-06-14 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
| AU2020204460A Active AU2020204460B2 (en) | 2013-06-12 | 2020-07-03 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2018204228A Active AU2018204228B2 (en) | 2013-06-12 | 2018-06-14 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
| AU2020204460A Active AU2020204460B2 (en) | 2013-06-12 | 2020-07-03 | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10266874B2 (2) |
| EP (2) | EP3587585B1 (2) |
| JP (2) | JP6600302B2 (2) |
| AU (3) | AU2014278537B2 (2) |
| CA (1) | CA2915033C (2) |
| ES (2) | ES2754177T3 (2) |
| WO (1) | WO2014200767A1 (2) |
Families Citing this family (146)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| WO2011127099A1 (en) | 2010-04-05 | 2011-10-13 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP4592400A3 (en) | 2012-10-17 | 2025-10-29 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| ES2966631T3 (es) | 2013-06-19 | 2024-04-23 | Univ California | Estructuras químicas para el suministro localizado de agentes terapéuticos |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| CN106659796B (zh) | 2014-03-14 | 2021-04-30 | 加利福尼亚大学董事会 | Tco缀合物和治疗剂递送方法 |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| JP6769969B2 (ja) | 2015-01-12 | 2020-10-14 | 10エックス ジェノミクス, インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| SG10202107053QA (en) * | 2015-07-17 | 2021-08-30 | Nanostring Technologies Inc | Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue |
| WO2017015097A1 (en) * | 2015-07-17 | 2017-01-26 | Nanostring Technologies, Inc. | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
| WO2017044983A1 (en) | 2015-09-10 | 2017-03-16 | Shasqi, Inc. | Bioorthogonal compositions |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ, インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| JP6954899B2 (ja) | 2015-12-04 | 2021-10-27 | 10エックス ゲノミクス,インコーポレイテッド | 核酸の解析のための方法及び組成物 |
| US20200408687A1 (en) * | 2016-03-25 | 2020-12-31 | The General Hospital Corporation | Polarization-Based Fluorescent Nucleic Acid Detection |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CA3029178C (en) * | 2016-06-30 | 2022-07-19 | Biois Co.,Ltd | Double-stranded nucleic acid signal probe and method for detecting target molecule using same |
| WO2018017469A1 (en) * | 2016-07-18 | 2018-01-25 | Yale University | Multiplex detection of intracellular or surface molecular targets in single cells |
| AU2017302300B2 (en) * | 2016-07-27 | 2023-08-17 | The Board Of Trustees Of The Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| CN109791157B (zh) * | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| IL266197B2 (en) | 2016-10-24 | 2024-03-01 | Geneinfosec Inc | Hiding information contained within nucleic acids |
| EP3554556B1 (en) * | 2016-12-19 | 2022-03-09 | Ventana Medical Systems, Inc. | Peptide nucleic acid conjugates |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| CN110520541B (zh) | 2017-02-13 | 2024-03-26 | 耶鲁大学 | 高通量单细胞多组学 |
| AU2018250312C1 (en) | 2017-04-07 | 2024-08-22 | Tambo, Inc. | Bioorthogonal compositions |
| JP6990456B2 (ja) * | 2017-05-02 | 2022-01-12 | 国立大学法人 東京大学 | 細胞またはその由来物の動的変化のモニタリング方法およびそれを用いた細胞分類方法 |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| US11084037B2 (en) * | 2017-09-25 | 2021-08-10 | Plexium, Inc. | Oligonucleotide encoded chemical libraries |
| US20200256862A1 (en) * | 2017-10-23 | 2020-08-13 | Massachusetts Institute Of Technology | Functionalized solid support |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| EP3724658A1 (en) * | 2017-12-12 | 2020-10-21 | 10X Genomics, Inc. | Systems and methods for single cell processing |
| JP7727385B2 (ja) | 2017-12-18 | 2025-08-21 | ヴェンタナ メディカル システムズ, インク. | ペプチド核酸コンジュゲート |
| JP7158642B2 (ja) * | 2018-01-16 | 2022-10-24 | 国立大学法人山梨大学 | 質量分析装置及び質量分析システム |
| KR101911859B1 (ko) * | 2018-02-02 | 2018-12-28 | 주식회사 바이오이즈 | 이중가닥 핵산 신호 프로브 및 이를 이용한 표적분자의 검출방법 |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| US20190249248A1 (en) | 2018-02-12 | 2019-08-15 | Nanostring Technologies, Inc. | Biomolecular probes and methods of detecting gene and protein expression |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
| CN110456034B (zh) * | 2018-05-07 | 2022-07-22 | 上海市第十人民医院 | 一种循环肿瘤细胞的检测方法 |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US12559787B2 (en) * | 2018-05-21 | 2026-02-24 | The Regents Of The University Of California | Single cell mapping and transcriptome analysis |
| US20200020419A1 (en) * | 2018-07-16 | 2020-01-16 | Flagship Pioneering Innovations Vi, Llc. | Methods of analyzing cells |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| CN113454238B (zh) | 2019-01-25 | 2025-02-11 | 哈佛大学校长及研究员协会 | 用于合成核酸的组合物和方法 |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| CN114174531A (zh) | 2019-02-28 | 2022-03-11 | 10X基因组学有限公司 | 用空间条码化寡核苷酸阵列对生物分析物进行概况分析 |
| EP3938538A1 (en) | 2019-03-15 | 2022-01-19 | 10X Genomics, Inc. | Methods for using spatial arrays for single cell sequencing |
| EP3887542A1 (en) | 2019-03-22 | 2021-10-06 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| JP7183899B2 (ja) * | 2019-03-25 | 2022-12-06 | 株式会社Jvcケンウッド | 分析装置及び分析方法 |
| CA3134973A1 (en) * | 2019-03-27 | 2020-10-01 | Fluidigm Canada Inc. | Lyophilized antibody panel |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| KR102406463B1 (ko) * | 2019-09-03 | 2022-06-10 | 재단법인 오송첨단의료산업진흥재단 | 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법 |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用 |
| WO2021091611A1 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
| ES3024383T3 (en) | 2019-11-13 | 2025-06-04 | 10X Genomics Inc | Generating capture probes for spatial analysis |
| EP4073246A4 (en) | 2019-12-12 | 2023-12-06 | President And Fellows Of Harvard College | COMPOSITIONS AND METHODS FOR LIGHT-DIRECTED BIOMOLECULAR BARCODING |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| US12577702B2 (en) | 2020-01-28 | 2026-03-17 | Plexium, Inc. | Oligonucleotide encoded chemical libraries, related systems, devices, and methods for detecting, analyzing, quantifying, and testing biologics/genetics |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| EP4105340A4 (en) * | 2020-02-14 | 2023-08-09 | Sony Group Corporation | ANALYTICAL METHOD, ANALYTICAL SYSTEM AND INTERFACE FOR ANALYSIS |
| US12399123B1 (en) | 2020-02-14 | 2025-08-26 | 10X Genomics, Inc. | Spatial targeting of analytes |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US20240018607A1 (en) * | 2020-03-31 | 2024-01-18 | Logicink Corporation | Molecular beacons |
| CN115997030A (zh) | 2020-04-30 | 2023-04-21 | 德迈逊科技有限公司 | 用于大分子操作的装置和方法 |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| WO2021243285A1 (en) * | 2020-05-29 | 2021-12-02 | Seer, Inc. | Systems and methods for rapid identification of proteins |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| CN116056765A (zh) | 2020-08-07 | 2023-05-02 | 坦伯公司 | 反式环辛烯生物正交剂及在癌症和免疫疗法中的用途 |
| US12553898B1 (en) | 2020-08-10 | 2026-02-17 | 10X Genomics, Inc. | Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| WO2022118862A1 (ja) * | 2020-12-01 | 2022-06-09 | 国立大学法人九州大学 | 生体分子構造検出用プローブ、生体分子構造検出用キット、及び生体分子構造の検出方法 |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| WO2022147296A1 (en) | 2020-12-30 | 2022-07-07 | 10X Genomics, Inc. | Cleavage of capture probes for spatial analysis |
| AU2022238446A1 (en) | 2021-03-18 | 2023-09-07 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
| EP4341847A1 (en) * | 2021-05-19 | 2024-03-27 | Leica Microsystems CMS GmbH | Marker, method and device for analyzing a biological sample |
| US20240255425A1 (en) * | 2021-05-19 | 2024-08-01 | Leica Microsystems Cms Gmbh | Marker, method and device for analyzing a biological sample |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| WO2023034789A1 (en) * | 2021-08-31 | 2023-03-09 | Becton, Dickinson And Company | Multiplexed proteomics analysis using oligonucleotide-conjugated antibodies |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| US20250144110A1 (en) * | 2022-01-28 | 2025-05-08 | Washington University | Compositions of autophagy modulating agents and uses thereof |
| WO2023250077A1 (en) | 2022-06-22 | 2023-12-28 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| EP4343326B1 (en) * | 2022-09-20 | 2025-03-12 | Miltenyi Biotec B.V. & Co. KG | Biological conjugates having an photogenerated acidic or basic releasable detection moiety on top of biological tissues |
| US20260098358A1 (en) * | 2022-09-22 | 2026-04-09 | Astrin Biosciences, Inc. | Tumor cell profiling-based therapeutic targeting of cancer |
| WO2024073524A2 (en) * | 2022-09-28 | 2024-04-04 | Massachusetts Institute Of Technology | High throughput stochastic bio-molecular sensor |
| EP4624590A3 (en) | 2022-12-30 | 2025-12-24 | 10X Genomics, Inc. | Methods, compositions, and kits for multiple barcoding and/or high-density spatial barcoding |
| KR102869006B1 (ko) * | 2023-03-14 | 2025-10-14 | 재단법인 아산사회복지재단 | 다중면역형광염색을 이용한 세포 시료에서 표적 단백질을 검출하는 방법 |
| WO2025049491A1 (en) * | 2023-08-29 | 2025-03-06 | Genopheno, Llc. | Novel sampling and genetic comparison and analysis of cannabis components for commercial differentiation |
| WO2025188805A1 (en) * | 2024-03-04 | 2025-09-12 | University Of Denver | Blood based rapid diagnostic system for brain hemorrhage detection and associated methods |
| US20250329467A1 (en) * | 2024-04-23 | 2025-10-23 | Delfi Diagnostics, Inc. | Workflows for discovery and deployment of diagnostic assays combining proteomic and genomic information |
| CN120254253A (zh) * | 2025-03-28 | 2025-07-04 | 无锡市人民医院 | 一种单细胞检测方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011143583A1 (en) * | 2010-05-13 | 2011-11-17 | Illumina, Inc. | Binding assays for markers |
Family Cites Families (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US5093268A (en) | 1988-04-28 | 1992-03-03 | Igen, Inc. | Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena |
| DE3920358A1 (de) | 1989-06-22 | 1991-01-17 | Behringwerke Ag | Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung |
| ES2259800T3 (es) | 1990-06-11 | 2006-10-16 | Gilead Sciences, Inc. | Procedimientos de uso de ligandos de acido nucleico. |
| ATE207080T1 (de) | 1991-11-25 | 2001-11-15 | Enzon Inc | Multivalente antigen-bindende proteine |
| US6136540A (en) | 1994-10-03 | 2000-10-24 | Ikonisys Inc. | Automated fluorescence in situ hybridization detection of genetic abnormalities |
| US5430175A (en) | 1993-11-30 | 1995-07-04 | Cornell Research Foundation, Inc. | Caged carboxyl compounds and use thereof |
| US5888829A (en) | 1994-11-08 | 1999-03-30 | Molecular Probes, Inc. | Photolabile caged ionophores and method of using in a membrane separation process |
| US5635608A (en) | 1994-11-08 | 1997-06-03 | Molecular Probes, Inc. | α-carboxy caged compounds |
| JPH11502617A (ja) | 1995-03-10 | 1999-03-02 | メソ スケール テクノロジーズ,エルエルシー | 多重アレイの多重特異的な電気化学発光試験 |
| US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| US5872243A (en) | 1995-06-30 | 1999-02-16 | Molecular Probes, Inc. | Caged nucleotides |
| US6043065A (en) | 1997-12-23 | 2000-03-28 | University Of Maryland Biotechnology Institute | Photosensitive organic compounds that release 2,5,-di(tert-butyl)hydroquinone upon illumination |
| US6472541B2 (en) | 1998-11-20 | 2002-10-29 | The Regents Of The University Of California | Protecting groups with increased photosensitivities |
| US20070009931A1 (en) * | 2000-08-04 | 2007-01-11 | Kirsch Wolff M | Negative correlation between IRP-2 and Transferrin receptor expression as a diagnostic of Alzheimer's disease |
| WO2002079514A1 (en) | 2001-01-10 | 2002-10-10 | The Trustees Of Boston College | Dna-bridged carbon nanotube arrays |
| EP1384022A4 (en) | 2001-04-06 | 2004-08-04 | California Inst Of Techn | NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES |
| WO2003048295A1 (en) | 2001-11-30 | 2003-06-12 | Fluidigm Corporation | Microfluidic device and methods of using same |
| US20030194711A1 (en) | 2002-04-10 | 2003-10-16 | Matthew Zapala | System and method for analyzing gene expression data |
| AU2003286739A1 (en) | 2002-10-28 | 2004-05-25 | University Of Washington | Wavelength tunable surface plasmon resonance sensor |
| EP1660858A4 (en) | 2003-07-21 | 2007-10-24 | Amplified Proteomics Inc | MULTIPLEX analyte |
| US20080318340A1 (en) | 2004-11-01 | 2008-12-25 | Tokyo Metropolitan Organization For Medical Resear | Method of Detecting Target Substances |
| WO2007076128A2 (en) | 2005-12-23 | 2007-07-05 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| JP5700911B2 (ja) | 2005-12-23 | 2015-04-15 | ナノストリング テクノロジーズ,インコーポレーテッド | 配向され、固定化された巨大分子を含む組成物とその製造法 |
| CA2653095C (en) | 2006-05-22 | 2013-07-16 | Nanostring Technologies, Inc. | Systems and methods for analyzing nanoreporters |
| CA2687292C (en) | 2007-04-10 | 2017-07-04 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
| US8865401B2 (en) * | 2007-12-14 | 2014-10-21 | The Johns Hopkins University | Purification and concentration of proteins and DNA from a complex sample using isotachophoresis and a device to perform the purification |
| US7923562B2 (en) * | 2008-06-16 | 2011-04-12 | The Board Of Trustees Of The Leland Stanford Junior University | Photocleavable linker methods and compositions |
| US9000142B2 (en) | 2008-07-07 | 2015-04-07 | Syntrix Biosystems, Inc. | Photocleavable sense-antisense complex |
| US8519115B2 (en) | 2008-08-14 | 2013-08-27 | Nanostring Technologies, Inc. | Stable nanoreporters |
| CA2749601C (en) | 2009-01-14 | 2020-07-21 | Stephen M. Hewitt | Ratio based biomarkers and methods of use thereof |
| WO2011047087A2 (en) | 2009-10-13 | 2011-04-21 | Nanostring Technologies, Inc. | Protein detection via nanoreporters |
| CA2789038A1 (en) * | 2010-02-08 | 2011-08-11 | Isis Pharmaceuticals, Inc. | Selective reduction of allelic variants |
| EP2516681B1 (en) | 2010-02-11 | 2017-10-18 | Nanostring Technologies, Inc | Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation |
| US20110229888A1 (en) | 2010-03-16 | 2011-09-22 | Hengen Paul N | Compositions and Methods for the Detection of Genomic Features |
| US9110079B2 (en) * | 2010-09-29 | 2015-08-18 | Biomerieux | Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS |
| US9758834B2 (en) | 2011-03-28 | 2017-09-12 | Nanostring Technologies, Inc. | Compositions and methods for diagnosing cancer |
| JP2013027387A (ja) | 2011-06-21 | 2013-02-07 | Tohoku Univ | 膵臓癌バイオマーカー |
| CA2839705A1 (en) | 2011-06-24 | 2012-12-27 | Nanostring Technologies, Inc. | Multivariate diagnostic assays and methods for using same |
| US20130095483A1 (en) * | 2011-10-17 | 2013-04-18 | Nuclea Biotechnologies, Inc. | Predictive biomarkers for breast cancer |
| WO2013078332A1 (en) | 2011-11-23 | 2013-05-30 | The General Hospital Corporation | Analyte detection using magnetic hall effect |
| US20140037620A1 (en) | 2012-06-29 | 2014-02-06 | British Columbia Cancer Agency Branch | Methods of Treating Breast Cancer with Gemcitabine Therapy |
| WO2014144325A1 (en) * | 2013-03-15 | 2014-09-18 | President And Fellows Of Harvard College | Methods and compositions for improving detection and/or capture of a target entity |
-
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- 2014-06-03 ES ES14810839T patent/ES2754177T3/es active Active
- 2014-06-03 ES ES19183482T patent/ES2887726T3/es active Active
- 2014-06-03 CA CA2915033A patent/CA2915033C/en active Active
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- 2020-07-03 AU AU2020204460A patent/AU2020204460B2/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011143583A1 (en) * | 2010-05-13 | 2011-11-17 | Illumina, Inc. | Binding assays for markers |
Non-Patent Citations (1)
| Title |
|---|
| AGASTI ET AL., "Photocleavable DNA Barcode-Antibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, (20121023), vol. 134, pages 18499 - 18502 * |
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