BG106105A - Метод за растителна трансформация - Google Patents

Метод за растителна трансформация Download PDF

Info

Publication number: BG106105A
Authority: BG; Bulgaria
Prior art keywords: plant; agrobacterium; tissue; target; transformation
Prior art date: 1999-04-19

Application number

BG106105A

Other languages

Bulgarian (bg)

English (en)

Inventor

Thierry Risacher

Melanie Craze

Original Assignee

Rhobio

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-04-19

Filing date

2001-11-13

Publication date

2002-06-28

2001-11-13 Application filed by Rhobio filed Critical Rhobio

2002-06-28 Publication of BG106105A publication Critical patent/BG106105A/bg

Links

238000011426 transformation method Methods 0.000 title claims abstract description 18
241000589158 Agrobacterium Species 0.000 claims abstract description 81
238000011081 inoculation Methods 0.000 claims abstract description 53
230000009261 transgenic effect Effects 0.000 claims abstract description 26
230000008929 regeneration Effects 0.000 claims abstract description 22
238000011069 regeneration method Methods 0.000 claims abstract description 22
241000196324 Embryophyta Species 0.000 claims description 151
238000000034 method Methods 0.000 claims description 71
230000009466 transformation Effects 0.000 claims description 51
206010020649 Hyperkeratosis Diseases 0.000 claims description 36
239000000463 material Substances 0.000 claims description 24
210000001161 mammalian embryo Anatomy 0.000 claims description 22
230000032459 dedifferentiation Effects 0.000 claims description 10
230000015572 biosynthetic process Effects 0.000 claims description 9
244000068988 Glycine max Species 0.000 claims description 8
239000003795 chemical substances by application Substances 0.000 claims description 8
230000006378 damage Effects 0.000 claims description 8
241000894007 species Species 0.000 claims description 8
235000010469 Glycine max Nutrition 0.000 claims description 7
240000002791 Brassica napus Species 0.000 claims description 6
244000020551 Helianthus annuus Species 0.000 claims description 6
235000003222 Helianthus annuus Nutrition 0.000 claims description 6
208000027418 Wounds and injury Diseases 0.000 claims description 6
238000000338 in vitro Methods 0.000 claims description 6
208000014674 injury Diseases 0.000 claims description 6
238000012258 culturing Methods 0.000 claims description 5
230000001902 propagating effect Effects 0.000 claims description 5
210000001672 ovary Anatomy 0.000 claims description 4
235000000832 Ayote Nutrition 0.000 claims description 2
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 claims description 2
235000004977 Brassica sinapistrum Nutrition 0.000 claims description 2
235000002566 Capsicum Nutrition 0.000 claims description 2
235000009854 Cucurbita moschata Nutrition 0.000 claims description 2
235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims description 2
241000209504 Poaceae Species 0.000 claims description 2
235000021536 Sugar beet Nutrition 0.000 claims description 2
235000015136 pumpkin Nutrition 0.000 claims description 2
240000004244 Cucurbita moschata Species 0.000 claims 1
239000006002 Pepper Substances 0.000 claims 1
235000016761 Piper aduncum Nutrition 0.000 claims 1
235000017804 Piper guineense Nutrition 0.000 claims 1
244000203593 Piper nigrum Species 0.000 claims 1
235000008184 Piper nigrum Nutrition 0.000 claims 1
230000004069 differentiation Effects 0.000 abstract description 4
210000001519 tissue Anatomy 0.000 description 70
108090000623 proteins and genes Proteins 0.000 description 51
210000002257 embryonic structure Anatomy 0.000 description 44
210000004027 cell Anatomy 0.000 description 34
230000014509 gene expression Effects 0.000 description 26
240000008042 Zea mays Species 0.000 description 24
OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 22
108020004707 nucleic acids Proteins 0.000 description 22
102000039446 nucleic acids Human genes 0.000 description 22
150000007523 nucleic acids Chemical class 0.000 description 22
235000002017 Zea mays subsp mays Nutrition 0.000 description 19
239000002609 medium Substances 0.000 description 19
238000010186 staining Methods 0.000 description 18
235000021307 Triticum Nutrition 0.000 description 17
241000209140 Triticum Species 0.000 description 17
235000013339 cereals Nutrition 0.000 description 17
238000002360 preparation method Methods 0.000 description 16
238000002474 experimental method Methods 0.000 description 15
238000002955 isolation Methods 0.000 description 15
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 14
235000009973 maize Nutrition 0.000 description 14
238000004458 analytical method Methods 0.000 description 12
238000012546 transfer Methods 0.000 description 12
239000008103 glucose Substances 0.000 description 11
238000004519 manufacturing process Methods 0.000 description 11
241000894006 Bacteria Species 0.000 description 10
239000012634 fragment Substances 0.000 description 10
230000001744 histochemical effect Effects 0.000 description 10
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
239000006870 ms-medium Substances 0.000 description 9
239000013612 plasmid Substances 0.000 description 9
108090000765 processed proteins & peptides Proteins 0.000 description 9
239000000725 suspension Substances 0.000 description 9
108700008625 Reporter Genes Proteins 0.000 description 8
238000011161 development Methods 0.000 description 8
230000018109 developmental process Effects 0.000 description 8
101150054900 gus gene Proteins 0.000 description 8
239000003550 marker Substances 0.000 description 7
102000004169 proteins and genes Human genes 0.000 description 7
210000001685 thyroid gland Anatomy 0.000 description 7
241000589155 Agrobacterium tumefaciens Species 0.000 description 6
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
239000007987 MES buffer Substances 0.000 description 6
230000001580 bacterial effect Effects 0.000 description 6
230000000408 embryogenic effect Effects 0.000 description 6
230000001939 inductive effect Effects 0.000 description 6
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 6
102000004196 processed proteins & peptides Human genes 0.000 description 6
230000001172 regenerating effect Effects 0.000 description 6
239000000243 solution Substances 0.000 description 6
239000013598 vector Substances 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
240000007594 Oryza sativa Species 0.000 description 5
235000007164 Oryza sativa Nutrition 0.000 description 5
241000700605 Viruses Species 0.000 description 5
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
235000005822 corn Nutrition 0.000 description 5
238000005286 illumination Methods 0.000 description 5
230000006698 induction Effects 0.000 description 5
229960000367 inositol Drugs 0.000 description 5
230000001105 regulatory effect Effects 0.000 description 5
235000009566 rice Nutrition 0.000 description 5
239000000523 sample Substances 0.000 description 5
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 5
241000588724 Escherichia coli Species 0.000 description 4
108091092195 Intron Proteins 0.000 description 4
238000010222 PCR analysis Methods 0.000 description 4
229930006000 Sucrose Natural products 0.000 description 4
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
108700019146 Transgenes Proteins 0.000 description 4
239000002253 acid Substances 0.000 description 4
230000005540 biological transmission Effects 0.000 description 4
239000012499 inoculation medium Substances 0.000 description 4
230000010354 integration Effects 0.000 description 4
230000000670 limiting effect Effects 0.000 description 4
230000001404 mediated effect Effects 0.000 description 4
230000008569 process Effects 0.000 description 4
239000005720 sucrose Substances 0.000 description 4
230000010474 transient expression Effects 0.000 description 4
235000011293 Brassica napus Nutrition 0.000 description 3
108020004414 DNA Proteins 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
241000209510 Liliopsida Species 0.000 description 3
229920002472 Starch Polymers 0.000 description 3
108020005202 Viral DNA Proteins 0.000 description 3
230000003115 biocidal effect Effects 0.000 description 3
229960004261 cefotaxime Drugs 0.000 description 3
GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 3
230000018044 dehydration Effects 0.000 description 3
238000006297 dehydration reaction Methods 0.000 description 3
238000004520 electroporation Methods 0.000 description 3
238000010195 expression analysis Methods 0.000 description 3
230000002363 herbicidal effect Effects 0.000 description 3
239000004009 herbicide Substances 0.000 description 3
238000001727 in vivo Methods 0.000 description 3
239000000411 inducer Substances 0.000 description 3
210000000056 organ Anatomy 0.000 description 3
230000037361 pathway Effects 0.000 description 3
229920003023 plastic Polymers 0.000 description 3
229920001983 poloxamer Polymers 0.000 description 3
239000007787 solid Substances 0.000 description 3
239000008107 starch Substances 0.000 description 3
235000019698 starch Nutrition 0.000 description 3
239000000126 substance Substances 0.000 description 3
101150043409 sul gene Proteins 0.000 description 3
108010053835 Catalase Proteins 0.000 description 2
108091026890 Coding region Proteins 0.000 description 2
208000035240 Disease Resistance Diseases 0.000 description 2
239000005562 Glyphosate Substances 0.000 description 2
240000005979 Hordeum vulgare Species 0.000 description 2
235000007340 Hordeum vulgare Nutrition 0.000 description 2
108091028043 Nucleic acid sequence Proteins 0.000 description 2
108700001094 Plant Genes Proteins 0.000 description 2
244000061456 Solanum tuberosum Species 0.000 description 2
235000002595 Solanum tuberosum Nutrition 0.000 description 2
244000062793 Sorghum vulgare Species 0.000 description 2
238000002105 Southern blotting Methods 0.000 description 2
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
229940088710 antibiotic agent Drugs 0.000 description 2
230000008901 benefit Effects 0.000 description 2
-1 biolafos Chemical compound 0.000 description 2
YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
108010031100 chloroplast transit peptides Proteins 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
235000013399 edible fruits Nutrition 0.000 description 2
230000000694 effects Effects 0.000 description 2
239000003623 enhancer Substances 0.000 description 2
230000002068 genetic effect Effects 0.000 description 2
XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
229940097068 glyphosate Drugs 0.000 description 2
230000012010 growth Effects 0.000 description 2
239000003906 humectant Substances 0.000 description 2
208000015181 infectious disease Diseases 0.000 description 2
239000002207 metabolite Substances 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
235000016709 nutrition Nutrition 0.000 description 2
230000008488 polyadenylation Effects 0.000 description 2
230000001954 sterilising effect Effects 0.000 description 2
238000004659 sterilization and disinfection Methods 0.000 description 2
238000003860 storage Methods 0.000 description 2
235000020238 sunflower seed Nutrition 0.000 description 2
JMSVCTWVEWCHDZ-UHFFFAOYSA-N syringic acid Chemical compound COC1=CC(C(O)=O)=CC(OC)=C1O JMSVCTWVEWCHDZ-UHFFFAOYSA-N 0.000 description 2
230000002123 temporal effect Effects 0.000 description 2
230000001131 transforming effect Effects 0.000 description 2
238000005406 washing Methods 0.000 description 2
KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 1
CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical class O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
HZWWPUTXBJEENE-UHFFFAOYSA-N 5-amino-2-[[1-[5-amino-2-[[1-[2-amino-3-(4-hydroxyphenyl)propanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoic acid Chemical compound C1CCC(C(=O)NC(CCC(N)=O)C(=O)N2C(CCC2)C(=O)NC(CCC(N)=O)C(O)=O)N1C(=O)C(N)CC1=CC=C(O)C=C1 HZWWPUTXBJEENE-UHFFFAOYSA-N 0.000 description 1
102000007469 Actins Human genes 0.000 description 1
108010085238 Actins Proteins 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
235000001674 Agaricus brunnescens Nutrition 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
241000589156 Agrobacterium rhizogenes Species 0.000 description 1
244000291564 Allium cepa Species 0.000 description 1
235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
244000003416 Asparagus officinalis Species 0.000 description 1
235000005340 Asparagus officinalis Nutrition 0.000 description 1
235000007319 Avena orientalis Nutrition 0.000 description 1
244000075850 Avena orientalis Species 0.000 description 1
108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 1
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
240000007124 Brassica oleracea Species 0.000 description 1
235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
239000005489 Bromoxynil Substances 0.000 description 1
229910000906 Bronze Inorganic materials 0.000 description 1
101100494773 Caenorhabditis elegans ctl-2 gene Proteins 0.000 description 1
102000012286 Chitinases Human genes 0.000 description 1
108010022172 Chitinases Proteins 0.000 description 1
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
241000219122 Cucurbita Species 0.000 description 1
101710088194 Dehydrogenase Proteins 0.000 description 1
SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
235000001950 Elaeis guineensis Nutrition 0.000 description 1
244000127993 Elaeis melanococca Species 0.000 description 1
101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 1
101001091269 Escherichia coli Hygromycin-B 4-O-kinase Proteins 0.000 description 1
244000004281 Eucalyptus maculata Species 0.000 description 1
101100112369 Fasciola hepatica Cat-1 gene Proteins 0.000 description 1
241000282326 Felis catus Species 0.000 description 1
241000206672 Gelidium Species 0.000 description 1
229920002148 Gellan gum Polymers 0.000 description 1
229930182566 Gentamicin Natural products 0.000 description 1
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
108010061711 Gliadin Proteins 0.000 description 1
229920001503 Glucan Polymers 0.000 description 1
239000005561 Glufosinate Substances 0.000 description 1
241000208818 Helianthus Species 0.000 description 1
241000238631 Hexapoda Species 0.000 description 1
108010033040 Histones Proteins 0.000 description 1
206010021929 Infertility male Diseases 0.000 description 1
206010069803 Injury associated with device Diseases 0.000 description 1
102000004195 Isomerases Human genes 0.000 description 1
108090000769 Isomerases Proteins 0.000 description 1
108010025815 Kanamycin Kinase Proteins 0.000 description 1
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
108060001084 Luciferase Proteins 0.000 description 1
239000005089 Luciferase Substances 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
208000007466 Male Infertility Diseases 0.000 description 1
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
240000005561 Musa balbisiana Species 0.000 description 1
235000018290 Musa x paradisiaca Nutrition 0.000 description 1
229930193140 Neomycin Natural products 0.000 description 1
101100005271 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cat-1 gene Proteins 0.000 description 1
244000061176 Nicotiana tabacum Species 0.000 description 1
235000002637 Nicotiana tabacum Nutrition 0.000 description 1
102000043276 Oncogene Human genes 0.000 description 1
108700020796 Oncogene Proteins 0.000 description 1
239000008896 Opium Substances 0.000 description 1
235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
244000046052 Phaseolus vulgaris Species 0.000 description 1
241000254064 Photinus pyralis Species 0.000 description 1
241000758706 Piperaceae Species 0.000 description 1
240000004713 Pisum sativum Species 0.000 description 1
235000010582 Pisum sativum Nutrition 0.000 description 1
101100121891 Pisum sativum SBEI gene Proteins 0.000 description 1
101100504555 Pisum sativum SBEII gene Proteins 0.000 description 1
229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
229920002531 Rubberwood Polymers 0.000 description 1
244000007853 Sarothamnus scoparius Species 0.000 description 1
235000007238 Secale cereale Nutrition 0.000 description 1
244000082988 Secale cereale Species 0.000 description 1
235000011684 Sorghum saccharatum Nutrition 0.000 description 1
108010039811 Starch synthase Proteins 0.000 description 1
101001091268 Streptomyces hygroscopicus Hygromycin-B 7''-O-kinase Proteins 0.000 description 1
229940100389 Sulfonylurea Drugs 0.000 description 1
108700005078 Synthetic Genes Proteins 0.000 description 1
102000004243 Tubulin Human genes 0.000 description 1
108090000704 Tubulin Proteins 0.000 description 1
241000607479 Yersinia pestis Species 0.000 description 1
235000007244 Zea mays Nutrition 0.000 description 1
229920002494 Zein Polymers 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000002411 adverse Effects 0.000 description 1
235000010419 agar Nutrition 0.000 description 1
238000000137 annealing Methods 0.000 description 1
238000010719 annulation reaction Methods 0.000 description 1
238000003556 assay Methods 0.000 description 1
244000052616 bacterial pathogen Species 0.000 description 1
230000004888 barrier function Effects 0.000 description 1
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
108091008324 binding proteins Proteins 0.000 description 1
229960000074 biopharmaceutical Drugs 0.000 description 1
239000010974 bronze Substances 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
230000023715 cellular developmental process Effects 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000008859 change Effects 0.000 description 1
238000002038 chemiluminescence detection Methods 0.000 description 1
229930002868 chlorophyll a Natural products 0.000 description 1
ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
229930002869 chlorophyll b Natural products 0.000 description 1
NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
210000003763 chloroplast Anatomy 0.000 description 1
238000010367 cloning Methods 0.000 description 1
239000012881 co-culture medium Substances 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
238000004040 coloring Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
235000009508 confectionery Nutrition 0.000 description 1
238000010276 construction Methods 0.000 description 1
KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
244000038559 crop plants Species 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000013461 design Methods 0.000 description 1
230000029087 digestion Effects 0.000 description 1
QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
125000005594 diketone group Chemical group 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000001035 drying Methods 0.000 description 1
239000002158 endotoxin Substances 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000007515 enzymatic degradation Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000001976 enzyme digestion Methods 0.000 description 1
210000002615 epidermis Anatomy 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000004720 fertilization Effects 0.000 description 1
KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
229940114124 ferulic acid Drugs 0.000 description 1
KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
235000001785 ferulic acid Nutrition 0.000 description 1
235000013376 functional food Nutrition 0.000 description 1
230000002538 fungal effect Effects 0.000 description 1
244000053095 fungal pathogen Species 0.000 description 1
230000000855 fungicidal effect Effects 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
229960002518 gentamicin Drugs 0.000 description 1
239000012869 germination medium Substances 0.000 description 1
108010050792 glutenin Proteins 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
235000008216 herbs Nutrition 0.000 description 1
239000010903 husk Substances 0.000 description 1
238000009396 hybridization Methods 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
150000002545 isoxazoles Chemical class 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
229930027917 kanamycin Natural products 0.000 description 1
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
229930182823 kanamycin A Natural products 0.000 description 1
OOYGSFOGFJDDHP-KMCOLRRFSA-N kanamycin A sulfate Chemical compound OS(O)(=O)=O.O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N OOYGSFOGFJDDHP-KMCOLRRFSA-N 0.000 description 1
229960002064 kanamycin sulfate Drugs 0.000 description 1
239000007788 liquid Substances 0.000 description 1
230000002101 lytic effect Effects 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
238000013507 mapping Methods 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
229960000485 methotrexate Drugs 0.000 description 1
235000019713 millet Nutrition 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
229960001027 opium Drugs 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
230000036961 partial effect Effects 0.000 description 1
239000002245 particle Substances 0.000 description 1
239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
230000008121 plant development Effects 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
239000000047 product Substances 0.000 description 1
238000011002 quantification Methods 0.000 description 1
230000006798 recombination Effects 0.000 description 1
238000005215 recombination Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
230000001850 reproductive effect Effects 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
238000010187 selection method Methods 0.000 description 1
210000000582 semen Anatomy 0.000 description 1
229960000268 spectinomycin Drugs 0.000 description 1
UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
210000000130 stem cell Anatomy 0.000 description 1
239000008223 sterile water Substances 0.000 description 1
229940124530 sulfonamide Drugs 0.000 description 1
150000003456 sulfonamides Chemical class 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
208000024891 symptom Diseases 0.000 description 1
YIBXWXOYFGZLRU-UHFFFAOYSA-N syringic aldehyde Natural products CC12CCC(C3(CCC(=O)C(C)(C)C3CC=3)C)C=3C1(C)CCC2C1COC(C)(C)C(O)C(O)C1 YIBXWXOYFGZLRU-UHFFFAOYSA-N 0.000 description 1
230000009885 systemic effect Effects 0.000 description 1
230000008685 targeting Effects 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
229940027257 timentin Drugs 0.000 description 1
239000003104 tissue culture media Substances 0.000 description 1
230000000451 tissue damage Effects 0.000 description 1
231100000827 tissue damage Toxicity 0.000 description 1
208000037816 tissue injury Diseases 0.000 description 1
QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
238000013518 transcription Methods 0.000 description 1
230000035897 transcription Effects 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
238000001890 transfection Methods 0.000 description 1
238000000844 transformation Methods 0.000 description 1
230000001052 transient effect Effects 0.000 description 1
238000003828 vacuum filtration Methods 0.000 description 1
MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
235000012141 vanillin Nutrition 0.000 description 1
235000013311 vegetables Nutrition 0.000 description 1
229940088594 vitamin Drugs 0.000 description 1
239000011782 vitamin Substances 0.000 description 1
235000013343 vitamin Nutrition 0.000 description 1
229930003231 vitamin Natural products 0.000 description 1
239000000080 wetting agent Substances 0.000 description 1
239000005019 zein Substances 0.000 description 1
229940093612 zein Drugs 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Cell Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

BG106105A 1999-04-19 2001-11-13 Метод за растителна трансформация BG106105A (bg)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP99420097		1999-04-19
PCT/EP2000/004177 WO2000063398A1 (en)	1999-04-19	2000-04-19	Plant transformation method

Publications (1)

Publication Number	Publication Date
BG106105A true BG106105A (bg)	2002-06-28

Family

ID=8242287

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
BG106105A BG106105A (bg)	1999-04-19	2001-11-13	Метод за растителна трансформация

Country Status (18)

Country	Link
US (3)	US7285705B1 (de)
EP (1)	EP1171621B1 (de)
JP (1)	JP2002541853A (de)
CN (1)	CN1347457A (de)
AR (1)	AR023576A1 (de)
AT (1)	ATE312183T1 (de)
AU (1)	AU775949B2 (de)
BG (1)	BG106105A (de)
BR (1)	BR0011140A (de)
CA (1)	CA2369428C (de)
CZ (1)	CZ301610B6 (de)
DE (1)	DE60024607T2 (de)
DK (1)	DK1171621T3 (de)
ES (1)	ES2252008T3 (de)
HU (1)	HU228627B1 (de)
IL (1)	IL145686A0 (de)
PL (1)	PL202067B1 (de)
WO (1)	WO2000063398A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6586661B1 (en)	1997-06-12	2003-07-01	North Carolina State University	Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
EP1724355A3 (de)	2000-08-30	2007-05-23	North Carolina State University	Transgene Pflanzen, die molekulare Decoys enthalten, die den Proteininhalt ändern
JP2004533807A (ja)	2000-11-07	2004-11-11	ノース・キャロライナ・ステイト・ユニヴァーシティ	プトレッシン−ｎ−メチルトランスフェラーゼプロモーター
CA2449920A1 (en)	2001-06-08	2002-12-19	Vector Tobacco Ltd.	Modifying nicotine and nitrosamine levels in tobacco
WO2006013072A2 (en)	2004-08-02	2006-02-09	Basf Plant Science Gmbh	Method for isolation of transcription termination sequences
EP2166100B1 (de)	2005-03-08	2012-07-18	BASF Plant Science GmbH	Expressionsverstärkende Intronsequenzen
CN100360677C (zh) *	2005-05-13	2008-01-09	吉林省农业科学院	一种植物转基因方法
AU2006260924B2 (en)	2005-06-23	2011-03-03	Basf Plant Science Gmbh	Improved methods for the production of stably transformed, fertile zea mays plants
EP2100962A1 (de)	2008-03-12	2009-09-16	Biogemma	Pflanzen mit verbesserter Widerstandsfähigkeit gegen Pathogene
WO2010144775A1 (en)	2009-06-11	2010-12-16	Syngenta Participations Ag	A method for the transient expression of nucleic acids in plants
CA2768955C (en)	2009-07-29	2018-07-17	Japan Tobacco Inc.	A method of gene introduction into triticum plant using agrobacterium, and a method of producing transformed triticum plant
EP2354232A1 (de)	2010-02-08	2011-08-10	Genoplante-Valor	Verbesserung der Kornfüllung von Getreide durch Modulation der Aktivität der NADH-Glutamat-Synthase
CA2804401A1 (en)	2010-07-09	2012-01-12	Genoplante-Valor	Preformed defense in plants
EP2619221A1 (de)	2010-09-23	2013-07-31	Genoplante-Valor	Gegen pilzkrankheitserreger resistente pflanzen und verfahren zu deren produktion
WO2013097940A1 (en)	2011-12-30	2013-07-04	Genoplante-Valor	Plants having a modulated content in seed proteins and method for production thereof
EP2687605A1 (de)	2012-07-19	2014-01-22	Biogemma	Verfahren zur Durchführung einer homologen Rekombination
EP2722391A1 (de)	2012-10-22	2014-04-23	Biogemma	Verfahren zur Anlagenverbesserung
EP3075741A1 (de)	2015-03-31	2016-10-05	Biogemma	Aspartate kinase - homoserine dehydrogenase für pflanzenverbesserung
EP3130675A1 (de)	2015-08-10	2017-02-15	Genoplante-Valor	Verfahren zur pflanzenoptimierung
CN106755083B (zh) *	2016-12-22	2019-06-14	中国科学院华南植物园	一种兜兰农杆菌子房注射法转基因方法
AR114025A1 (es)	2017-10-31	2020-07-15	Limagrain Europe	Trigo que comprende alelos restauradores de la fertilidad masculina
JP7611151B2 (ja)	2019-02-06	2025-01-09	ヴィルモラン・エ・シエ	細胞質雄性不稔を司る新たな遺伝子
EP4419693A1 (de)	2021-10-19	2024-08-28	Limagrain Europe	Gezielte genintegration in pflanzen
CN114223426A (zh) *	2021-12-27	2022-03-25	中国热带农业科学院热带生物技术研究所	一种单子叶植物叶片液体注射方法
EP4311430A1 (de)	2022-07-28	2024-01-31	Limagrain Europe	Chlortolurontoleranzgen und verfahren zur verwendung davon

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5102796A (en) *	1983-04-15	1992-04-07	Lubrizol Genetics, Inc.	Plant structural gene expression
US4658082A (en) *	1984-07-25	1987-04-14	Atlantic Richfield Company	Method for producing intact plants containing foreign DNA
US6664109B2 (en) *	1985-01-17	2003-12-16	Calgene Llc	Transformation system with Ti or Ri plasmid
US5024944A (en) *	1986-08-04	1991-06-18	Lubrizol Genetics, Inc.	Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
EP0672752B1 (de) *	1993-09-03	2004-05-26	Japan Tobacco Inc.	Verfahren zur transformation von monocotyledonen mittels des schildes von unreifen embryonen
EP0856060A2 (de) *	1996-06-21	1998-08-05	Monsanto Company	Verfahren zur herstellung stabil-transformiertes fertiles weizen, durch agrobakterium vermittelte transformation und davon abgeleiteten zusammensetzungen
ES2151338B1 (es) *	1997-03-05	2001-07-01	Inia	Procedimiento de transformacion genetica de plantas adultas de especies leñosas.
AR015873A1 (es) *	1997-06-13	2001-05-30	Shell Int Research	Un proceso para producir material vegetal modificado geneticamente que comprende una o mas secuencias de adn de interes establemente incorporadas
FI104907B (fi) *	1997-09-18	2000-04-28	Univ Helsinki Licensing	Rypsin Agrobacterium-välitteinen transformaatio
US5994624A (en) *	1997-10-20	1999-11-30	Cotton Incorporated	In planta method for the production of transgenic plants

2000
- 2000-04-19 HU HU0200926A patent/HU228627B1/hu not_active IP Right Cessation
- 2000-04-19 WO PCT/EP2000/004177 patent/WO2000063398A1/en not_active Ceased
- 2000-04-19 PL PL351895A patent/PL202067B1/pl unknown
- 2000-04-19 EP EP00935000A patent/EP1171621B1/de not_active Expired - Lifetime
- 2000-04-19 CZ CZ20013744A patent/CZ301610B6/cs not_active IP Right Cessation
- 2000-04-19 AU AU50650/00A patent/AU775949B2/en not_active Ceased
- 2000-04-19 BR BR0011140-6A patent/BR0011140A/pt not_active IP Right Cessation
- 2000-04-19 ES ES00935000T patent/ES2252008T3/es not_active Expired - Lifetime
- 2000-04-19 AR ARP000101883A patent/AR023576A1/es active IP Right Grant
- 2000-04-19 US US09/959,137 patent/US7285705B1/en not_active Expired - Lifetime
- 2000-04-19 JP JP2000612477A patent/JP2002541853A/ja not_active Withdrawn
- 2000-04-19 CN CN00806271A patent/CN1347457A/zh active Pending
- 2000-04-19 DE DE60024607T patent/DE60024607T2/de not_active Expired - Lifetime
- 2000-04-19 DK DK00935000T patent/DK1171621T3/da active
- 2000-04-19 AT AT00935000T patent/ATE312183T1/de active
- 2000-04-19 IL IL14568600A patent/IL145686A0/xx unknown
- 2000-04-19 CA CA2369428A patent/CA2369428C/en not_active Expired - Fee Related
2001
- 2001-11-13 BG BG106105A patent/BG106105A/bg unknown
2007
- 2007-08-10 US US11/837,143 patent/US7803988B2/en not_active Expired - Fee Related
2010
- 2010-09-27 US US12/891,056 patent/US8115061B2/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
DE60024607D1 (de)	2006-01-12
PL202067B1 (pl)	2009-05-29
BR0011140A (pt)	2002-02-26
US20080047036A1 (en)	2008-02-21
CZ20013744A3 (cs)	2002-01-16
PL351895A1 (en)	2003-06-30
CA2369428C (en)	2011-06-28
ATE312183T1 (de)	2005-12-15
IL145686A0 (en)	2002-06-30
EP1171621B1 (de)	2005-12-07
US7803988B2 (en)	2010-09-28
US20110078827A1 (en)	2011-03-31
AR023576A1 (es)	2002-09-04
DE60024607T2 (de)	2006-08-10
WO2000063398A1 (en)	2000-10-26
CZ301610B6 (cs)	2010-04-28
HUP0200926A2 (en)	2002-07-29
ES2252008T3 (es)	2006-05-16
CN1347457A (zh)	2002-05-01
JP2002541853A (ja)	2002-12-10
US7285705B1 (en)	2007-10-23
AU5065000A (en)	2000-11-02
HU228627B1 (hu)	2013-04-29
EP1171621A1 (de)	2002-01-16
AU775949B2 (en)	2004-08-19
CA2369428A1 (en)	2000-10-26
US8115061B2 (en)	2012-02-14
DK1171621T3 (da)	2006-03-13

Publication	Publication Date	Title
US8115061B2 (en)	2012-02-14	Plant transformation method
AU738153B2 (en)	2001-09-13	Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
US6420630B1 (en)	2002-07-16	Methods for tissue culturing and transforming elite inbreds of Zea mays L.
US20120192318A1 (en)	2012-07-26	Transformation system for Camelina sativa
US20080229447A1 (en)	2008-09-18	Transformation of immature soybean seeds through organogenesis
AU2007204597B2 (en)	2013-01-24	Method of producing transgenic graminaceous cells and plants
JP2018503392A (ja)	2018-02-08	遺伝子一過性発現により完全な植物において部位特異的改変を行うための方法
Celikkol Akcay et al.	2009	Agrobacterium tumefaciens-mediated genetic transformation of a recalcitrant grain legume, lentil (Lens culinaris Medik)
Tyagi et al.	2007	Regeneration and Agrobacterium-mediated transformation of a popular indica rice variety, ADT39
El-Siddig et al.	2011	Agrobacterium-mediated transformation of tomato plants expressing defensin gene
Begum et al.	2025	INHERITED ALTERATION UTILIZING THE COTYLEDONARY NODE OF PEANUTS (ARACHIS HYPOGAEA L.) AS A TRANSPLANT AND A PROMOTER-LESS GUS:: EPSPS FUSION GENE-DERIVED VECTOR.
Song et al.	2012	Use of petal explants for successful transformation of Dendranthema× grandiflorum Kitamura'Orlando'mediated by Agrobacterium tumefaciens
Firoozabady et al.	1995	Agrobacterium-mediated transformation
Firsov et al.	1996	Agrobacterial transformation of Actinidia kolomicta
KR100363122B1 (ko)	2002-12-05	유전자 조작을 통한 마늘 식물체의 형질전환 방법 및 형질전환 마늘
Otani et al.	2001	Transgenic Sweet Potato (Ipomoea batatas L. Lam.)