BR0014864A - Métodos de produzir células progenitoras diferenciadas e células tronco embriÈnicas defeituosas em linhagem - Google Patents
Métodos de produzir células progenitoras diferenciadas e células tronco embriÈnicas defeituosas em linhagemInfo
- Publication number
- BR0014864A BR0014864A BR0014864-4A BR0014864A BR0014864A BR 0014864 A BR0014864 A BR 0014864A BR 0014864 A BR0014864 A BR 0014864A BR 0014864 A BR0014864 A BR 0014864A
- Authority
- BR
- Brazil
- Prior art keywords
- cells
- progenitor cells
- differentiated progenitor
- methods
- embryonic stem
- Prior art date
Links
- 210000000130 stem cell Anatomy 0.000 title abstract 7
- 230000002950 deficient Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 210000001671 embryonic stem cell Anatomy 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 4
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 230000004075 alteration Effects 0.000 abstract 1
- 210000002459 blastocyst Anatomy 0.000 abstract 1
- 230000024245 cell differentiation Effects 0.000 abstract 1
- 238000002659 cell therapy Methods 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 210000001161 mammalian embryo Anatomy 0.000 abstract 1
- 210000000056 organ Anatomy 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A—HUMAN NECESSITIES
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- A61P13/00—Drugs for disorders of the urinary system
- A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
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- A—HUMAN NECESSITIES
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- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
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- A—HUMAN NECESSITIES
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
- A61P25/16—Anti-Parkinson drugs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
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- A—HUMAN NECESSITIES
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- A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/02—Non-specific cardiovascular stimulants, e.g. drugs for syncope, antihypotensives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Neurology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Neurosurgery (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Diabetes (AREA)
- Immunology (AREA)
- Cardiology (AREA)
- Physical Education & Sports Medicine (AREA)
- Virology (AREA)
- Psychology (AREA)
- Obesity (AREA)
- Transplantation (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
Abstract
"MéTODOS DE PRODUZIR CéLULAS PROGENITORAS DIFERENCIADAS E CéLULAS TRONCO EMBRIÈNICAS DEFEITUOSAS EM LINHAGEM". Um método aperfeiçoado de produzir células progenitoras aperfeiçoadas compreendendo obtendo células de massa de célula interna de um blastócisto e induzindo diferenciação das células de massa de célula interna para produzir células progenitoras diferenciadas. As células progenitoras diferenciadas podem ser transfectadas tais que exista além disso, anulação ou alteração de um gene desejado. As células progenitoras diferenciadas são úteis em terapia de célula e como uma fonte I de células para a produção de tecidos e orgãos para transplantação. Também fornecido é um método para produzir uma célula de tronco embriónica humana defeituosa em linhagem.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15955099P | 1999-10-15 | 1999-10-15 | |
| PCT/US2000/028285 WO2001029206A1 (en) | 1999-10-15 | 2000-10-13 | Methods of producing differentiated progenitor cells and lineage-defective embryonic stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0014864A true BR0014864A (pt) | 2002-11-19 |
Family
ID=22573023
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0014864-4A BR0014864A (pt) | 1999-10-15 | 2000-10-13 | Métodos de produzir células progenitoras diferenciadas e células tronco embriÈnicas defeituosas em linhagem |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20050265976A1 (pt) |
| EP (1) | EP1226239A4 (pt) |
| JP (1) | JP2003512052A (pt) |
| CN (1) | CN1413250A (pt) |
| AU (1) | AU782385B2 (pt) |
| BR (1) | BR0014864A (pt) |
| CA (1) | CA2388497A1 (pt) |
| IL (2) | IL149043A0 (pt) |
| MX (1) | MXPA02003733A (pt) |
| NZ (1) | NZ518191A (pt) |
| WO (1) | WO2001029206A1 (pt) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7015037B1 (en) | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
| US10638734B2 (en) | 2004-01-05 | 2020-05-05 | Abt Holding Company | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof |
| US8252280B1 (en) | 1999-08-05 | 2012-08-28 | Regents Of The University Of Minnesota | MAPC generation of muscle |
| IL148418A0 (en) * | 1999-09-07 | 2002-09-12 | Advanced Cell Tech Inc | Method for generating immune-compatible cells and tissues using nuclear transfer techniques |
| US20020142397A1 (en) | 2000-12-22 | 2002-10-03 | Philippe Collas | Methods for altering cell fate |
| US7491534B2 (en) | 2000-12-22 | 2009-02-17 | Kirin Holdings Kabushiki Kaisha | Methods for altering cell fate to generate T-cells specific for an antigen of interest |
| KR20030088023A (ko) | 2001-01-02 | 2003-11-15 | 스템론 인크. | 미리 선별된 면역형 및(또는) 유전자형을 갖는 동형접합성간세포 군집의 제조 방법, 그로부터 유래된 이식에 적합한세포, 및 이들을 사용하는 재료 및 방법 |
| CA2438501C (en) | 2001-02-14 | 2014-09-16 | Leo T. Furcht | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof |
| IL159578A0 (en) * | 2001-07-06 | 2004-06-01 | Geron Corp | Messenchymal cells and osteoblasts from human embryonic stem cell |
| WO2003075648A1 (es) * | 2002-03-13 | 2003-09-18 | Fundacion Ivi Para El Estudio De La Reproduccion Humana (Fivier) | Metodos de produccion de lineas celulares |
| CA2515552C (en) | 2002-12-27 | 2012-09-18 | Mitsubishi Heavy Industries, Ltd. | Method and apparatus of cultivating multipotent stem cell |
| RU2265446C2 (ru) * | 2003-05-14 | 2005-12-10 | Григорьянц Рубен Исаакович | Имплантат для лечения болезни паркинсона |
| EP1812554A4 (en) * | 2004-11-04 | 2008-10-08 | Advanced Cell Tech Inc | DERIVATION OF EMBRYONAL STEM CELLS |
| US7893315B2 (en) | 2004-11-04 | 2011-02-22 | Advanced Cell Technology, Inc. | Derivation of embryonic stem cells and embryo-derived cells |
| RU2283122C1 (ru) * | 2005-03-16 | 2006-09-10 | Государственное учреждение Научный центр акушерства, гинекологии и перинатологии Российской академии медицинских наук | Способ лечения императивного недержания мочи |
| EP1878789A4 (en) * | 2005-04-14 | 2008-08-27 | Japan As Represented By Genera | STEM CELL DERIVED FROM FAT AND USE THEREOF |
| ES2933478T3 (es) | 2005-08-03 | 2023-02-09 | Astellas Inst For Regenerative Medicine | Métodos mejorados de reprogramación de células somáticas animales |
| AU2007238660C1 (en) | 2006-04-14 | 2013-11-07 | Astellas Institute For Regenerative Medicine | Hemangio-colony forming cells |
| EP2029724A4 (en) | 2006-05-03 | 2010-03-24 | Advanced Cell Tech Inc | DERIVATION FROM EMBRYONAL STEM CELLS AND EMBRYO-DERIVED CELLS |
| WO2008063675A2 (en) | 2006-11-24 | 2008-05-29 | Regents Of The University Of Minnesota | Endodermal progenitor cells |
| KR20100014504A (ko) * | 2007-02-23 | 2010-02-10 | 어드밴스드 셀 테크놀로지, 인코포레이티드 | 분화된 세포를 리프로그래밍하고, 리프로그래밍된 세포로부터 동물 및 배아 줄기세포를 생성시키기 위한 고도로 효율적인 방법 |
| US20110086424A1 (en) | 2008-05-06 | 2011-04-14 | Advanced Cell Technology, Inc. | Methods for producing enucleated erythroid cells derived from pluripotent stem cells |
| BRPI0912201A2 (pt) | 2008-05-06 | 2019-09-24 | Advanced Cell Tech Inc | células formadoras de colônias de hemangios e células de hemangio não enxertantes |
| US10386360B2 (en) | 2009-03-13 | 2019-08-20 | University Of Central Florida Research Foundation, Inc. | Bio-microelectromechanical system transducer and associated methods |
| US8815584B1 (en) | 2009-04-23 | 2014-08-26 | University Of Central Florida Research Foundation, Inc. | Method of co-culturing mammalian muscle cells and motoneurons |
| US9404140B1 (en) | 2009-11-03 | 2016-08-02 | The University Of Central Florida Research Foundation, Inc. | Patterned cardiomyocyte culture on microelectrode array |
| CA2798777C (en) | 2010-04-23 | 2016-09-06 | University Of Central Florida Research Foundation, Inc. | Formation of neuromuscular junctions in a defined system |
| JP2010193910A (ja) * | 2010-06-14 | 2010-09-09 | Mitsubishi Heavy Ind Ltd | 多能性幹細胞の培養方法、多能性幹細胞の培養システム、及び多能性幹細胞培養装置 |
| WO2012022786A1 (de) | 2010-08-20 | 2012-02-23 | Hirschmann Automation And Control Gmbh | Erweiterung für das simple network management protocol (snmp) um informationen über den status von set-pdus zu ermitteln |
| EP3636286A1 (en) * | 2011-05-18 | 2020-04-15 | The Regents of The University of California | Compositions and methods for treating retinal diseases |
| US10865383B2 (en) | 2011-07-12 | 2020-12-15 | Lineage Cell Therapeutics, Inc. | Methods and formulations for orthopedic cell therapy |
| US8961956B2 (en) | 2011-11-30 | 2015-02-24 | Ocata Therapeutics, Inc. | Mesenchymal stromal cells and uses related thereto |
| PL2785359T3 (pl) | 2011-11-30 | 2018-12-31 | Astellas Institute For Regenerative Medicine | Mezenchymalne komórki zrębowe i związane z nimi zastosowania |
| CN104471059B (zh) | 2012-07-12 | 2018-04-17 | 珠海横琴爱姆斯坦生物科技有限公司 | 人胚胎干细胞衍生的间充质样干细胞、方法及其应用 |
| JP2015524674A (ja) | 2012-08-17 | 2015-08-27 | ユニバーシティ オブ セントラル フロリダ リサーチ ファウンデーション,インコーポレイテッド | 哺乳動物系の機能的なインビトロ細胞モデルについての方法、システム、および組成物 |
| CA2896053A1 (en) | 2012-12-21 | 2014-06-26 | Ocata Therapeutics, Inc. | Methods for production of platelets from pluripotent stem cells and compositions thereof |
| CA2899445C (en) | 2013-01-30 | 2023-04-04 | University Of Central Florida Research Foundation, Inc. | Devices and systems for mimicking heart function |
| US10935541B2 (en) | 2014-08-07 | 2021-03-02 | University Of Central Florida Research Foundation, Inc. | Devices and methods comprising neuromuscular junctions |
| EP3350313B1 (en) * | 2015-09-15 | 2025-11-26 | Agency For Science, Technology And Research (A*star) | Derivation of liver organoids from human pluripotent stem cells |
| KR20240037175A (ko) | 2016-05-16 | 2024-03-21 | 인타르시아 세라퓨틱스 인코포레이티드 | 글루카곤-수용체 선택적 폴리펩티드 및 이들의 이용 방법 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE483795T1 (de) | 1992-10-28 | 2010-10-15 | Neurospheres Holdings Ltd | Biologische faktoren und neuronale stammzellen |
| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| US5532156A (en) * | 1993-10-08 | 1996-07-02 | The United States Of America As Represented By The Secretary Of Agriculture | Hepatocyte cell line derived from the epiblast of pig blastocysts |
| US5843780A (en) * | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| BR9711204A (pt) * | 1996-08-19 | 1999-08-17 | Univ Massachusetts | Linhagens de c-lulas embriÄnicas ou similares s indiferenciadas produzidas por transplante nuclear de esp-cies hibridas |
| US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| IL133786A0 (en) * | 1997-07-03 | 2001-04-30 | Univ Massachusetts | Cloning using donor nuclei from non-serum starved, differentiated cells |
| JP2001518290A (ja) * | 1997-09-29 | 2001-10-16 | ポイント セラピューティクス, インコーポレイテッド | インビトロでの造血細胞の刺激 |
| CN1299408A (zh) * | 1998-03-02 | 2001-06-13 | 马萨诸塞大学 | 通过异种间的核移植产生的胚细胞或干细胞样细胞系 |
| NZ517609A (en) * | 1999-09-14 | 2004-02-27 | Univ Massachusetts | Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions |
| US6576464B2 (en) * | 2000-11-27 | 2003-06-10 | Geron Corporation | Methods for providing differentiated stem cells |
| WO2004050826A2 (en) * | 2002-11-29 | 2004-06-17 | Technion Research & Development Foundation Ltd. | Method of dynamically culturing embryonic stem cells |
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2000
- 2000-10-13 JP JP2001532190A patent/JP2003512052A/ja active Pending
- 2000-10-13 EP EP00973497A patent/EP1226239A4/en not_active Ceased
- 2000-10-13 WO PCT/US2000/028285 patent/WO2001029206A1/en not_active Ceased
- 2000-10-13 CN CN00817477A patent/CN1413250A/zh active Pending
- 2000-10-13 NZ NZ518191A patent/NZ518191A/en not_active IP Right Cessation
- 2000-10-13 BR BR0014864-4A patent/BR0014864A/pt not_active Application Discontinuation
- 2000-10-13 AU AU12000/01A patent/AU782385B2/en not_active Ceased
- 2000-10-13 MX MXPA02003733A patent/MXPA02003733A/es unknown
- 2000-10-13 IL IL14904300A patent/IL149043A0/xx unknown
- 2000-10-13 CA CA002388497A patent/CA2388497A1/en not_active Abandoned
-
2002
- 2002-04-09 IL IL149043A patent/IL149043A/en unknown
-
2004
- 2004-12-29 US US11/025,893 patent/US20050265976A1/en not_active Abandoned
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2008
- 2008-05-16 US US12/152,779 patent/US8753884B2/en not_active Expired - Fee Related
-
2014
- 2014-05-07 US US14/272,365 patent/US20140248700A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| NZ518191A (en) | 2004-01-30 |
| JP2003512052A (ja) | 2003-04-02 |
| EP1226239A4 (en) | 2003-02-12 |
| US20090104697A1 (en) | 2009-04-23 |
| AU1200001A (en) | 2001-04-30 |
| IL149043A (en) | 2010-11-30 |
| WO2001029206A1 (en) | 2001-04-26 |
| IL149043A0 (en) | 2002-11-10 |
| MXPA02003733A (es) | 2003-10-14 |
| US20140248700A1 (en) | 2014-09-04 |
| EP1226239A1 (en) | 2002-07-31 |
| US8753884B2 (en) | 2014-06-17 |
| AU782385B2 (en) | 2005-07-21 |
| US20050265976A1 (en) | 2005-12-01 |
| CA2388497A1 (en) | 2001-04-26 |
| CN1413250A (zh) | 2003-04-23 |
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