BR0015382A - Método para sintetização do ácido nucléico - Google Patents
Método para sintetização do ácido nucléicoInfo
- Publication number
- BR0015382A BR0015382A BR0015382-6A BR0015382A BR0015382A BR 0015382 A BR0015382 A BR 0015382A BR 0015382 A BR0015382 A BR 0015382A BR 0015382 A BR0015382 A BR 0015382A
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- primer
- present
- synthesis method
- oligonucleotide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Patente de Invenção: "MéTODO PARA SINTETIZAçãO DO áCIDO NUCLéICO". A presente invenção refere-se a um oligonucleotídeo tendo uma nova estrutura e um método de sintetização de ácido nucléico empregando-se o mesmo como um iniciador. Este oligonucleotídeo é fornecido no lado 5' do iniciador com uma seq³ência de nucleotídeo substancialmente a mesma como a região sintetizada com este iniciador como a origem de síntese. A presente invenção realiza a síntese de ácido nucléico com base em uma reação isotérmica com uma constituição simples de reagentes. Além disso, a presente invenção fornece um método de sintetização de ácido nucléico altamente específico nas bases deste método de sintetização de ácido nucléico.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JPPCT/JP99/06213 | 1999-11-08 | ||
| PCT/JP1999/006213 WO2000028082A1 (fr) | 1998-11-09 | 1999-11-08 | Procede de synthese d'acide nucleique |
| PCT/JP2000/001919 WO2001034790A1 (en) | 1999-11-08 | 2000-03-28 | Method for synthesizing nucleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR0015382A true BR0015382A (pt) | 2002-07-02 |
| BR0015382B1 BR0015382B1 (pt) | 2014-04-29 |
Family
ID=99552039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0015382-6A BR0015382B1 (pt) | 1999-11-08 | 2000-03-28 | Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia |
Country Status (3)
| Country | Link |
|---|---|
| BR (1) | BR0015382B1 (pt) |
| CA (1) | CA2390309C (pt) |
| NO (1) | NO331732B1 (pt) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
| CA2903874C (en) | 2013-03-15 | 2024-02-20 | Theranos, Inc. | Nucleic acid amplification |
| JP2016521120A (ja) | 2013-03-15 | 2016-07-21 | セラノス， インコーポレイテッド | 核酸増幅 |
-
2000
- 2000-03-28 CA CA2390309A patent/CA2390309C/en not_active Expired - Lifetime
- 2000-03-28 BR BRPI0015382-6A patent/BR0015382B1/pt active IP Right Grant
-
2002
- 2002-05-07 NO NO20022171A patent/NO331732B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BR0015382B1 (pt) | 2014-04-29 |
| CA2390309C (en) | 2012-09-25 |
| CA2390309A1 (en) | 2001-05-17 |
| NO331732B1 (no) | 2012-03-12 |
| NO20022171L (no) | 2002-07-04 |
| NO20022171D0 (no) | 2002-05-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25G | Requested change of headquarter approved |
Owner name: EIKEN KAGAKU KABUSHIKI KAISHA (JP) Free format text: SEDE ALTERADA CONFORME SOLICITADO NA PETICAO NO 020080153598/RJ DE 12/12/2008. |
|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] |
Free format text: ANULACAO DA PUBLICACAO POR TER SIDO INDEVIDA REF, A RPI 2228 DE 17/09/2013 |
|
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 29/04/2014, OBSERVADAS AS CONDICOES LEGAIS. |