BR0015382A - Método para sintetização do ácido nucléico - Google Patents

Método para sintetização do ácido nucléico

Info

Publication number
BR0015382A
BR0015382A BR0015382-6A BR0015382A BR0015382A BR 0015382 A BR0015382 A BR 0015382A BR 0015382 A BR0015382 A BR 0015382A BR 0015382 A BR0015382 A BR 0015382A
Authority
BR
Brazil
Prior art keywords
nucleic acid
primer
present
synthesis method
oligonucleotide
Prior art date
Application number
BR0015382-6A
Other languages
English (en)
Other versions
BR0015382B1 (pt
Inventor
Tsugunori Notomi
Tetsu Hase
Original Assignee
Eiken Chemical
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=14237231&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR0015382(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/JP1999/006213 external-priority patent/WO2000028082A1/ja
Application filed by Eiken Chemical filed Critical Eiken Chemical
Publication of BR0015382A publication Critical patent/BR0015382A/pt
Publication of BR0015382B1 publication Critical patent/BR0015382B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Patente de Invenção: "MéTODO PARA SINTETIZAçãO DO áCIDO NUCLéICO". A presente invenção refere-se a um oligonucleotídeo tendo uma nova estrutura e um método de sintetização de ácido nucléico empregando-se o mesmo como um iniciador. Este oligonucleotídeo é fornecido no lado 5' do iniciador com uma seq³ência de nucleotídeo substancialmente a mesma como a região sintetizada com este iniciador como a origem de síntese. A presente invenção realiza a síntese de ácido nucléico com base em uma reação isotérmica com uma constituição simples de reagentes. Além disso, a presente invenção fornece um método de sintetização de ácido nucléico altamente específico nas bases deste método de sintetização de ácido nucléico.
BRPI0015382-6A 1999-11-08 2000-03-28 Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia BR0015382B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPPCT/JP99/06213 1999-11-08
PCT/JP1999/006213 WO2000028082A1 (fr) 1998-11-09 1999-11-08 Procede de synthese d'acide nucleique
PCT/JP2000/001919 WO2001034790A1 (en) 1999-11-08 2000-03-28 Method for synthesizing nucleic acid

Publications (2)

Publication Number Publication Date
BR0015382A true BR0015382A (pt) 2002-07-02
BR0015382B1 BR0015382B1 (pt) 2014-04-29

Family

ID=14237231

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0015382-6A BR0015382B1 (pt) 1999-11-08 2000-03-28 Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia

Country Status (9)

Country Link
KR (1) KR100612551B1 (pt)
CN (2) CN100393875C (pt)
BR (1) BR0015382B1 (pt)
CA (1) CA2390309C (pt)
IL (1) IL149446A0 (pt)
NO (1) NO331732B1 (pt)
RU (1) RU2252964C2 (pt)
WO (1) WO2001034790A1 (pt)
ZA (1) ZA200203293B (pt)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
ES2546450T3 (es) * 2000-04-07 2015-09-23 Eiken Kagaku Kabushiki Kaisha Método para amplificar y detectar un ácido nucleico usando ácido nucleico bicatenario como molde
ATE437225T1 (de) 2000-09-19 2009-08-15 Eiken Chemical Verfahren zur polynukleotidsynthese
KR100806680B1 (ko) * 2003-11-21 2008-02-26 한국표준과학연구원 Ｐｃｒ용 열순환 반응기의 성능시험용 다중 ｐｃｒ 조성물
JP5818018B2 (ja) * 2009-03-26 2015-11-18 厦門艾徳生物医薬科技有限公司 核酸増幅を用いる環状プライマー及びその応用
CN101671674B (zh) * 2009-03-27 2010-09-22 郑立谋 一种用于核酸扩增的环形引物及其应用
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US10457936B2 (en) 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
TWI600766B (zh) * 2012-08-09 2017-10-01 財團法人工業技術研究院 用於偵測一目標核苷酸序列中之一特定區域的一突變及／或多形性的套組
WO2014142850A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
JP2016521120A (ja) * 2013-03-15 2016-07-21 セラノス， インコーポレイテッド 核酸増幅
AU2014233152A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
CN106414764B (zh) * 2013-11-22 2020-10-30 欧雷恩诊断公司 通过基于链侵入的扩增的核酸检测
CN106414765A (zh) 2013-12-20 2017-02-15 Illumina公司 在片段化的基因组dna样品中保留基因组连接信息
CN106661600B (zh) 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
CN114369596B (zh) 2014-12-15 2025-07-08 塞弗德公司 底数大于2的指数核酸扩增
WO2018132939A1 (zh) * 2017-01-17 2018-07-26 中国科学院过程工程研究所 一种恒温条件下合成核酸的方法
WO2019051732A1 (zh) * 2017-09-14 2019-03-21 中科芯瑞(苏州)生物科技有限公司 一种恒温条件下合成核酸的方法及试剂盒
CA3118000A1 (en) 2018-10-29 2020-05-07 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
US12325883B2 (en) 2019-01-15 2025-06-10 Neogen Food Safety Us Holdco Corporation Loop-mediated isothermal amplification primers for Shiga toxin-producing E. coli (STEC) detection
US12559804B2 (en) 2020-02-17 2026-02-24 Neogen Food Safety Us Holdco Corporation Loop-mediated isothermal amplification primers for vibrio parahaemolyticus detection and uses thereof
CA3186580A1 (en) 2020-06-12 2021-12-16 Sherlock Biosciences, Inc. Crispr-based sars-cov-2 detection
CN116075596A (zh) 2020-08-07 2023-05-05 牛津纳米孔科技公开有限公司 鉴定核酸条形码的方法
CN115109840B (zh) * 2021-03-18 2025-08-12 厦门大学 一种分析目标核酸中突变的存在及其类型的方法
EP4105327A4 (en) * 2021-04-29 2023-01-18 Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences PROCESS FOR THE PREPARATION OF CONSTANT TEMPERATURE NUCLEIC ACIDS AND KIT AND USE THEREOF
CN113201583B (zh) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
KR102908488B1 (ko) 2022-11-10 2026-01-07 (주)레보스케치 황색포도상구균 감염 진단을 위한 rt-lamp용 조성물 및 이의 용도

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2005589C (en) * 1988-12-16 2001-02-06 Akzo Nobel Nv Self-sustained, sequence replication system
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
CA2080305A1 (en) * 1991-10-11 1993-04-12 Linda M. Western Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
WO1993017127A1 (en) * 1992-02-20 1993-09-02 The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Boomerand dna amplification
FR2721945B1 (fr) * 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
EP1231281B1 (en) * 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism

Also Published As

Publication number Publication date
BR0015382B1 (pt) 2014-04-29
CN1876843B (zh) 2012-09-05
NO20022171D0 (no) 2002-05-07
KR100612551B1 (ko) 2006-08-11
IL149446A0 (en) 2002-11-10
RU2252964C2 (ru) 2005-05-27
NO20022171L (no) 2002-07-04
CA2390309C (en) 2012-09-25
CN1420928A (zh) 2003-05-28
CN100393875C (zh) 2008-06-11
NO331732B1 (no) 2012-03-12
WO2001034790A1 (en) 2001-05-17
KR20020064896A (ko) 2002-08-10
RU2002115268A (ru) 2004-01-27
ZA200203293B (en) 2003-03-26
CN1876843A (zh) 2006-12-13
CA2390309A1 (en) 2001-05-17

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Legal Events

Date Code Title Description
B25G Requested change of headquarter approved

Owner name: EIKEN KAGAKU KABUSHIKI KAISHA (JP)

Free format text: SEDE ALTERADA CONFORME SOLICITADO NA PETICAO NO 020080153598/RJ DE 12/12/2008.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULACAO DA PUBLICACAO POR TER SIDO INDEVIDA REF, A RPI 2228 DE 17/09/2013

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 29/04/2014, OBSERVADAS AS CONDICOES LEGAIS.